Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00003
(711 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g23900.1 68418.m02807 60S ribosomal protein L13 (RPL13D) 93 2e-19
At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) / br... 90 1e-18
At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) / br... 90 1e-18
At3g48960.1 68416.m05348 60S ribosomal protein L13 (RPL13C) 60S ... 82 4e-16
At2g02060.1 68415.m00141 calcium-dependent protein kinase-relate... 29 2.3
At5g65110.1 68418.m08191 acyl-CoA oxidase (ACX2) identical to ac... 27 9.3
At5g12280.1 68418.m01444 hypothetical protein 27 9.3
At2g22690.1 68415.m02689 expressed protein 27 9.3
>At5g23900.1 68418.m02807 60S ribosomal protein L13 (RPL13D)
Length = 206
Score = 92.7 bits (220), Expect = 2e-19
Identities = 62/199 (31%), Positives = 92/199 (46%), Gaps = 2/199 (1%)
Frame = +2
Query: 20 KGNNMIPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPT 199
K NN+IP+ HF K WQ +VKTWFNQPAR+ RR+ R LRP+V T
Sbjct: 2 KHNNVIPSSHFRKHWQNYVKTWFNQPARKTRRRVARQKKAVKIFPRPTSGPLRPVVHGQT 61
Query: 200 VRYHTKVRAGRGFTLREIR-PQIEPSICPNDWNCCRSP*TQQVC*IIANQCSKNKGIQSA 376
++Y+ KVRAG+GFTL E++ I + P + + + + K ++
Sbjct: 62 LKYNMKVRAGKGFTLEELKVAGIPKKLAPTIGISVDHRRKNRSLEGLQSNVQRLKTYKAK 121
Query: 377 SYTVPERQKGAEG*GQ*RRT*AGYT-ATWSINASSTACS*ISCKTITEDEKNFKAYQYLR 553
P R + + A T ++ + + +T D K FKAY +R
Sbjct: 122 LVVFPRRSRQVKAGDSTPEELANATQVQGDYMPIASVKAAMELVKLTADLKAFKAYDKIR 181
Query: 554 GARSIAKLVGIRAKRLKDA 610
R+ A+ G RAKR +A
Sbjct: 182 LERTNARHAGARAKRAAEA 200
Score = 72.9 bits (171), Expect = 2e-13
Identities = 34/67 (50%), Positives = 51/67 (76%), Gaps = 1/67 (1%)
Frame = +3
Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
A TIGI+VD RR+N+S+E LQ NVQR+K Y+A+L++FP + ++V G++ EE ATQ+
Sbjct: 89 APTIGISVDHRRKNRSLEGLQSNVQRLKTYKAKLVVFPRRSRQVKAGDSTPEELANATQV 148
Query: 456 RGPLMPV 476
+G MP+
Sbjct: 149 QGDYMPI 155
>At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 90.2 bits (214), Expect = 1e-18
Identities = 40/79 (50%), Positives = 51/79 (64%)
Frame = +2
Query: 20 KGNNMIPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPT 199
K NN+IPNGHF K WQ +VKTWFNQPAR+ RR+ R LRP+V T
Sbjct: 2 KHNNVIPNGHFKKHWQNYVKTWFNQPARKTRRRIARQKKAVKIFPRPTSGPLRPVVHGQT 61
Query: 200 VRYHTKVRAGRGFTLREIR 256
++Y+ KVR G+GFTL E++
Sbjct: 62 LKYNMKVRTGKGFTLEELK 80
Score = 75.4 bits (177), Expect = 4e-14
Identities = 35/72 (48%), Positives = 52/72 (72%), Gaps = 1/72 (1%)
Frame = +3
Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
A TIGIAVD RR+N+S+E LQ NVQR+K Y+ +L++FP + +KV G++ EE ATQ+
Sbjct: 89 APTIGIAVDHRRKNRSLEGLQTNVQRLKTYKTKLVIFPRRARKVKAGDSTPEELANATQV 148
Query: 456 RGPLMPVQQPAP 491
+G +P+ + P
Sbjct: 149 QGDYLPIVREKP 160
Score = 28.3 bits (60), Expect = 5.3
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 509 ITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDA 610
+T + K+FKA+ +R R+ + G RAKR +A
Sbjct: 167 LTSEMKSFKAFDKIRLERTNKRHAGARAKRAAEA 200
>At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 90.2 bits (214), Expect = 1e-18
Identities = 40/79 (50%), Positives = 51/79 (64%)
Frame = +2
Query: 20 KGNNMIPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPT 199
K NN+IPNGHF K WQ +VKTWFNQPAR+ RR+ R LRP+V T
Sbjct: 2 KHNNVIPNGHFKKHWQNYVKTWFNQPARKTRRRIARQKKAVKIFPRPTSGPLRPVVHGQT 61
Query: 200 VRYHTKVRAGRGFTLREIR 256
++Y+ KVR G+GFTL E++
Sbjct: 62 LKYNMKVRTGKGFTLEELK 80
Score = 75.4 bits (177), Expect = 4e-14
Identities = 35/72 (48%), Positives = 52/72 (72%), Gaps = 1/72 (1%)
Frame = +3
Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
A TIGIAVD RR+N+S+E LQ NVQR+K Y+ +L++FP + +KV G++ EE ATQ+
Sbjct: 89 APTIGIAVDHRRKNRSLEGLQTNVQRLKTYKTKLVIFPRRARKVKAGDSTPEELANATQV 148
Query: 456 RGPLMPVQQPAP 491
+G +P+ + P
Sbjct: 149 QGDYLPIVREKP 160
Score = 28.3 bits (60), Expect = 5.3
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 509 ITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDA 610
+T + K+FKA+ +R R+ + G RAKR +A
Sbjct: 167 LTSEMKSFKAFDKIRLERTNKRHAGARAKRAAEA 200
>At3g48960.1 68416.m05348 60S ribosomal protein L13 (RPL13C) 60S
ribosomal protein L13 (BBC1), Arabidopsis thaliana,
gb:X75162
Length = 206
Score = 81.8 bits (193), Expect = 4e-16
Identities = 37/79 (46%), Positives = 49/79 (62%)
Frame = +2
Query: 20 KGNNMIPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPT 199
K NN+IPNGHF K W+ +VKT FNQPA + RR+ R +RP+V T
Sbjct: 2 KHNNVIPNGHFKKKWENYVKTSFNQPAMKTRRRIARQNKAVKIFPRPTAGPIRPVVHAQT 61
Query: 200 VRYHTKVRAGRGFTLREIR 256
+ Y+ KVRAG+GFTL E++
Sbjct: 62 LTYNMKVRAGKGFTLEELK 80
Score = 66.9 bits (156), Expect = 1e-11
Identities = 35/95 (36%), Positives = 60/95 (63%), Gaps = 1/95 (1%)
Frame = +3
Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFPKGKKVLK-GEANEEERKLATQL 455
A TIGI+VD R+N+S+E Q NVQR+K Y+A+L++FP+ + +K G++ ++E ATQ+
Sbjct: 89 APTIGISVDHHRKNRSLEGFQTNVQRLKTYKAKLVIFPRCARTVKVGDSAQQELANATQV 148
Query: 456 RGPLMPVQQPAPKSVARPSLKMKRTSKLINT*EEL 560
+ MP+ + P +K+ KL N +++
Sbjct: 149 QVDHMPIVREMP---TMELVKLTSDMKLFNAYDKI 180
>At2g02060.1 68415.m00141 calcium-dependent protein kinase-related /
CDPK-related contains TIGRFAM TIGR01557: myb-like
DNA-binding domain, SHAQKYF class; contains Pfam
PF00249: Myb-like DNA-binding domain; similar to CDPK
substrate protein 1; CSP1 (GI:6942190) [Mesembryanthemum
crystallinum].
Length = 626
Score = 29.5 bits (63), Expect = 2.3
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = -1
Query: 150 ATALAFFILFCFLRYRRAGWLNQV 79
A L FFI FCF YR +G+L V
Sbjct: 266 AVLLLFFICFCFSLYRTSGYLRIV 289
>At5g65110.1 68418.m08191 acyl-CoA oxidase (ACX2) identical to
acyl-CoA oxidase [Arabidopsis thaliana] GI:3044212
Length = 692
Score = 27.5 bits (58), Expect = 9.3
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +2
Query: 590 AKRLKDAAENPDDVTKAPTA 649
AK L DA E PD VT+AP A
Sbjct: 658 AKELVDAFELPDHVTRAPIA 677
>At5g12280.1 68418.m01444 hypothetical protein
Length = 419
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +3
Query: 324 SVESLQINVQRIKEYRARLILFPKGKKVLKGEA 422
+V+SL NV +KE + I FP K+ L+G+A
Sbjct: 362 TVQSLSENVASLKEKISGEIQFPTNKQKLRGKA 394
>At2g22690.1 68415.m02689 expressed protein
Length = 381
Score = 27.5 bits (58), Expect = 9.3
Identities = 12/45 (26%), Positives = 26/45 (57%)
Frame = +3
Query: 237 SLFVKLGRRLNPVFARTIGIAVDPRRRNKSVESLQINVQRIKEYR 371
S+ V G ++ P F + + + + R+ +++E L + V+ +EYR
Sbjct: 273 SVTVAAGSQVLPTFLKYLNVLPEKRKEWQTMEQLLVPVELSEEYR 317
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,035,169
Number of Sequences: 28952
Number of extensions: 283475
Number of successful extensions: 729
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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