SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP01_F_D03
         (348 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g33330.1 68417.m04740 glycogenin glucosyltransferase (glycoge...    28   2.0  
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro...    27   2.6  
At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family prote...    27   3.4  
At3g11800.1 68416.m01446 expressed protein                             27   4.5  
At4g18330.2 68417.m02719 eukaryotic translation initiation facto...    26   6.0  
At4g18330.1 68417.m02718 eukaryotic translation initiation facto...    26   6.0  
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S...    26   6.0  
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S...    26   6.0  
At2g34220.1 68415.m04187 hypothetical protein contains Pfam prof...    26   6.0  
At4g35920.3 68417.m05107 expressed protein contains Pfam profile...    26   7.9  
At4g35920.2 68417.m05106 expressed protein contains Pfam profile...    26   7.9  
At4g35920.1 68417.m05105 expressed protein contains Pfam profile...    26   7.9  

>At4g33330.1 68417.m04740 glycogenin glucosyltransferase
           (glycogenin)-related similar to glycogenin
           glucosyltransferase (glycogenin-1) (EC 2.4.1.186) from
           Homo sapiens [SP|P46976], Mus musculus [SP|Q9R062],
           Oryctolagus cuniculus [GI:165513]; contains Pfam profile
           PF01501: Glycosyl transferase family 8
          Length = 593

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 21/72 (29%), Positives = 38/72 (52%)
 Frame = +1

Query: 109 TKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLPKTMTFKYP 288
           TK +  +++E+I    +   +TKI M   ++  +  +    + V + F  + K   FK+ 
Sbjct: 87  TKLEAPSFMEEILTRGLG--KTKIGMVNMEECDLTNWKRYGETVHIHFERVSKL--FKWQ 142

Query: 289 DLFEKSINEEEE 324
           DLF + I+EEEE
Sbjct: 143 DLFPEWIDEEEE 154


>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
           protein GI:1279562 from [Medicago sativa]
          Length = 557

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 25/69 (36%), Positives = 35/69 (50%)
 Frame = +1

Query: 133 LEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLPKTMTFKYPDLFEKSIN 312
           L+K    P  D+ TK+++ K KKDV+   V KE  VK     +PK +          S +
Sbjct: 20  LKKGKREPEDDIDTKVSLKKQKKDVIAA-VQKEKAVK----KVPKKVESSDD---SDSES 71

Query: 313 EEEEHAKSL 339
           EEEE AK +
Sbjct: 72  EEEEKAKKV 80


>At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family protein
           low similarity to hydrolases from Rhodococcus sp. EtbD2
           GI:3273241, EtbD1 GI:3273239; contains Pfam profile
           PF00561: hydrolase, alpha/beta fold family
          Length = 314

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 12/49 (24%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
 Frame = +1

Query: 31  NFWMKLVRPHPKQLPNIVHFH---CSMEMTKYDIKNYLEKIYEVPVVDV 168
           NFW+   +P  K  PN++  H    +     YD+   L + + + + D+
Sbjct: 38  NFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRYFNLYIPDL 86


>At3g11800.1 68416.m01446 expressed protein
          Length = 246

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 15/60 (25%), Positives = 29/60 (48%)
 Frame = +1

Query: 85  HFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLP 264
           H H S+     ++  +  K+ E+ ++ + T  +   F+ D  KGY++     K A  +LP
Sbjct: 71  HRHLSLNGNS-EVAVFRPKVDEITLLTINTS-SSSSFRPDASKGYMVAFAGAKYAARSLP 128


>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
           subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
           putative similar to SP|Q09130 Eukaryotic translation
           initiation factor 2 gamma subunit (eIF-2- gamma)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF00009: Elongation factor Tu GTP binding domain;
           isoform predicted to contain a TG non-consensus acceptor
           splice site.
          Length = 471

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
 Frame = +1

Query: 148 EVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVA-FVTLPKTMTFKYPDL 294
           + P+V V  ++   K+  DVV  Y++K+  + V  FV+ PK +  +  D+
Sbjct: 217 DAPIVPVSAQL---KYNIDVVSEYIVKKIPIPVRDFVSPPKMIVIRSFDV 263


>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
           subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
           putative similar to SP|Q09130 Eukaryotic translation
           initiation factor 2 gamma subunit (eIF-2- gamma)
           {Schizosaccharomyces pombe}; contains Pfam profile
           PF00009: Elongation factor Tu GTP binding domain;
           isoform predicted to contain a TG non-consensus acceptor
           splice site.
          Length = 284

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
 Frame = +1

Query: 148 EVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVA-FVTLPKTMTFKYPDL 294
           + P+V V  ++   K+  DVV  Y++K+  + V  FV+ PK +  +  D+
Sbjct: 217 DAPIVPVSAQL---KYNIDVVSEYIVKKIPIPVRDFVSPPKMIVIRSFDV 263


>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
           family protein similar to SP|P07814 Bifunctional
           aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
           synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
           Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
           ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
           tRNA synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain
          Length = 530

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 18/81 (22%), Positives = 34/81 (41%)
 Frame = +1

Query: 46  LVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVI 225
           ++RP    +  I+      E+ K  +KN    ++  P V  + K ++  F  +V      
Sbjct: 81  ILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKS 140

Query: 226 KEDDVKVAFVTLPKTMTFKYP 288
            + D++V     P + T  YP
Sbjct: 141 GKSDLEVPIAIRPTSETVMYP 161


>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
           family protein similar to SP|P07814 Bifunctional
           aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
           synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
           Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
           ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
           tRNA synthetase class II core domain (G, H, P, S and T),
           PF03129: Anticodon binding domain
          Length = 530

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 18/81 (22%), Positives = 34/81 (41%)
 Frame = +1

Query: 46  LVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVI 225
           ++RP    +  I+      E+ K  +KN    ++  P V  + K ++  F  +V      
Sbjct: 81  ILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKS 140

Query: 226 KEDDVKVAFVTLPKTMTFKYP 288
            + D++V     P + T  YP
Sbjct: 141 GKSDLEVPIAIRPTSETVMYP 161


>At2g34220.1 68415.m04187 hypothetical protein contains Pfam
           profiles PF04780: Protein of unknown function (DUF629),
           PF04781: Protein of unknown function (DUF627)
          Length = 718

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = +1

Query: 223 IKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAK 333
           +  DDVK + +TL K++  K+P +  K +  +  + K
Sbjct: 627 VPNDDVKKSLLTLRKSLKEKFPLIDSKILQNKSTYEK 663


>At4g35920.3 68417.m05107 expressed protein contains Pfam profile
           PF04749: Protein of unknown function, DUF614; isoform
           contains AT-acceptor splice site at intron 8
          Length = 421

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +1

Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
           V+  ++K++  + A   L KT++  YP+L F +++  E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198


>At4g35920.2 68417.m05106 expressed protein contains Pfam profile
           PF04749: Protein of unknown function, DUF614; isoform
           contains AT-acceptor splice site at intron 8
          Length = 421

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +1

Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
           V+  ++K++  + A   L KT++  YP+L F +++  E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198


>At4g35920.1 68417.m05105 expressed protein contains Pfam profile
           PF04749: Protein of unknown function, DUF614; isoform
           contains AT-acceptor splice site at intron 8
          Length = 421

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
 Frame = +1

Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
           V+  ++K++  + A   L KT++  YP+L F +++  E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,222,342
Number of Sequences: 28952
Number of extensions: 100889
Number of successful extensions: 228
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 429398688
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -