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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20010
         (420 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd...    54   5e-08
At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd...    53   7e-08
At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd...    52   2e-07
At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd...    49   1e-06
At5g46570.1 68418.m05734 protein kinase family protein contains ...    32   0.18 
At1g64330.1 68414.m07290 myosin heavy chain-related similar to m...    28   3.0  

>At3g12290.1 68416.m01534 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 299

 Score = 53.6 bits (123), Expect = 5e-08
 Identities = 27/75 (36%), Positives = 46/75 (61%)
 Frame = +3

Query: 33  AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 212
           A+II G  +A +I +++ ++V  L  K  G  P LA+V VG R+DS  Y+  K KA  ++
Sbjct: 9   AKIIDGKAIAHTIRSEIAEEVRGLSEK-HGKVPGLAVVIVGSRKDSQTYVNTKRKACAEV 67

Query: 213 GIAAEHIRLPRDITK 257
           GI +  + LP ++++
Sbjct: 68  GIKSFDVGLPEEVSE 82



 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 24/52 (46%), Positives = 36/52 (69%)
 Frame = +2

Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
           +L++K+  LN +P VHGI+VQ+PL   H  + H +  A+S DKDVDG + +N
Sbjct: 84  DLISKVHELNSNPDVHGILVQLPLPK-HINEEH-ILGAISIDKDVDGFHPLN 133


>At4g00620.1 68417.m00086 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 360

 Score = 53.2 bits (122), Expect = 7e-08
 Identities = 24/52 (46%), Positives = 36/52 (69%)
 Frame = +2

Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
           E+L  ++  N+ PSVHGI+VQ+PL S   +D   + +AVS +KDVDG + +N
Sbjct: 144 EVLKSVSGFNDDPSVHGILVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLN 193



 Score = 52.4 bits (120), Expect = 1e-07
 Identities = 27/72 (37%), Positives = 43/72 (59%)
 Frame = +3

Query: 33  AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 212
           A +I G  VA  I +++  +V+R++    G  P LA++ VG R+DS  Y+R K KA + +
Sbjct: 69  AIVIDGKAVAKKIRDEITIEVSRMKES-IGVIPGLAVILVGDRKDSATYVRNKKKACDSV 127

Query: 213 GIAAEHIRLPRD 248
           GI +  +RL  D
Sbjct: 128 GIKSFEVRLAED 139


>At4g00600.1 68417.m00084 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 310

 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 23/52 (44%), Positives = 36/52 (69%)
 Frame = +2

Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
           E+L  ++  N+ PSVHG++VQ+PL S   +D   + +AVS +KDVDG + +N
Sbjct: 94  EVLKYVSGFNDDPSVHGVLVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLN 143


>At2g38660.1 68415.m04748 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
           precursor (C1-THF synthase) [Includes:
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
           Formyltetrahydrofolate synthetase (EC 6.3.4.3)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           NAD(P)-binding domain, PF00763: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, catalytic domain
          Length = 352

 Score = 49.2 bits (112), Expect = 1e-06
 Identities = 27/73 (36%), Positives = 41/73 (56%)
 Frame = +3

Query: 39  IISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGI 218
           +I G  +A  I   +  +V +++ K  G  P LA+V VG + DS  Y+R K+KA E+ GI
Sbjct: 65  VIDGNVIAEEIRTKIISEVGKMK-KAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGI 123

Query: 219 AAEHIRLPRDITK 257
            +    LP D T+
Sbjct: 124 KSVLAELPEDCTE 136



 Score = 41.1 bits (92), Expect = 3e-04
 Identities = 17/52 (32%), Positives = 33/52 (63%)
 Frame = +2

Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415
           ++++ +   NE  S+HGI+VQ+PL     ++  ++ + V  +KDVDG + +N
Sbjct: 138 QIISVLRKFNEDTSIHGILVQLPLPQH--LNESKILNMVRLEKDVDGFHPLN 187


>At5g46570.1 68418.m05734 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 489

 Score = 31.9 bits (69), Expect = 0.18
 Identities = 19/59 (32%), Positives = 29/59 (49%)
 Frame = +3

Query: 42  ISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGI 218
           ++G +V   I+N    ++  LR   +GF P   IV  GG +  NV  R KL+    + I
Sbjct: 26  VNGDQVDQEIQNFKEFELNELRKATNGFSPS-CIVSEGGEKAPNVVYRGKLEGNHLVAI 83


>At1g64330.1 68414.m07290 myosin heavy chain-related similar to
           myosin heavy chain (GI:1850913) [Entamoeba histolytica];
           similar to Intracellular protein transport protein USO1
           (Swiss-Prot:P25386) [Saccharomyces cerevisiae]
          Length = 555

 Score = 27.9 bits (59), Expect = 3.0
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +3

Query: 51  IEVAGSIENDLRQQVTRLRSKWSGFEPRLA 140
           +EVAG  E+DL Q++  ++ +  G E  LA
Sbjct: 190 LEVAGETESDLNQKLEDVKKERDGLEAELA 219


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,137,647
Number of Sequences: 28952
Number of extensions: 142737
Number of successful extensions: 358
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 347
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 354
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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