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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0034
         (682 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote...    44   9e-05
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H...    43   2e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS...    41   7e-04
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17...    39   0.003
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS...    37   0.011
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote...    37   0.014
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS...    34   0.076
At1g78910.1 68414.m09199 pseudouridine synthase family protein l...    29   2.8  
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote...    29   3.8  
At3g49540.1 68416.m05414 expressed protein                             28   5.0  
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi...    27   8.7  
At1g34180.1 68414.m04239 no apical meristem (NAM) family protein...    27   8.7  

>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
           (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
           heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
           (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
           17 (19), 7995 (1989))
          Length = 157

 Score = 44.0 bits (99), Expect = 9e-05
 Identities = 32/93 (34%), Positives = 50/93 (53%), Gaps = 8/93 (8%)
 Frame = +2

Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391
           D+     EE+ V+  DG I+ + G+    +EEK D+   + R   +FTRR+ LPE    E
Sbjct: 66  DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125

Query: 392 SVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 490
            +++ +  +GVLSV  P KVP      + I I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156


>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
           (HSP22.0-ER) identical to endomembrane-localized small
           heat shock protein GI:511795 from [Arabidopsis thaliana]
          Length = 195

 Score = 43.2 bits (97), Expect = 2e-04
 Identities = 31/104 (29%), Positives = 56/104 (53%), Gaps = 8/104 (7%)
 Frame = +2

Query: 203 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 358
           K   +  ++ LD+     +E+ ++  + G + V G   + EEKK DQ   + R   +F R
Sbjct: 76  KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135

Query: 359 RYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 490
           ++ LP+    ESV+++L  +GVL++   +  P  V+G R + IA
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178


>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
           (HSP17.4-CI) identical to 17.4 kDa class I heat shock
           protein SP:P19036 from [Arabidopsis thaliana]
          Length = 156

 Score = 41.1 bits (92), Expect = 7e-04
 Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
 Frame = +2

Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391
           DV     EE+ V+  DG I+ + G+    +EEK D    + R   +F RR+ LPE    E
Sbjct: 65  DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124

Query: 392 SVESRLSSDGVLSVIAPR 445
            V++ +  +GVLSV  P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141


>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
           (HSP17.7-CII) identical to heat shock protein 17.6A
           GI:3256075 from [Arabidopsis thaliana]
          Length = 156

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 34/126 (26%), Positives = 58/126 (46%), Gaps = 9/126 (7%)
 Frame = +2

Query: 137 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHE 313
           SR Y R  + +AA   D    +    D +   +D+     +EI V+   +  +VV GK +
Sbjct: 33  SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88

Query: 314 -EKKDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEG 469
            + K+  G     + R+   F R++ LP+    E + S   +DGVL V  P+  PP  + 
Sbjct: 89  RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147

Query: 470 ERKIPI 487
            + I +
Sbjct: 148 PKTIQV 153


>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
           (HSP18.1-CI) identical to 18.2 kDa class I heat shock
           protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
           contains Pfam profile: PF00011 Hsp20/alpha crystallin
           family
          Length = 161

 Score = 37.1 bits (82), Expect = 0.011
 Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
 Frame = +2

Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391
           D+     EE+ V+  D  ++ + G+    +EEK D+   + R   +F RR+ LPE    E
Sbjct: 68  DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127

Query: 392 SVESRLSSDGVLSVIAPR 445
            V++ +  +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144


>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
           (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
           crystallin family; identified in Scharf, K-D., et al,
           Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 153

 Score = 36.7 bits (81), Expect = 0.014
 Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
 Frame = +2

Query: 236 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 391
           D+     EE+ V+   D  + + G +H EK+D++    R      QFTRR+ LPE    +
Sbjct: 62  DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121

Query: 392 SVESRLSSDGVLSVIAPR 445
            V++ +  +GVL+V  P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138


>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
           (HSP17.8-CI) similar to 17.5 kDa class I heat shock
           protein SP:P04793 from [Glycine max]; contains Pfam
           PF00011: Hsp20/alpha crystallin family
          Length = 157

 Score = 34.3 bits (75), Expect = 0.076
 Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
 Frame = +2

Query: 236 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 391
           D+     EE+ V+  D  ++ + G+     EEK+D    + R   QF+R++ LPE    +
Sbjct: 64  DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123

Query: 392 SVESRLSSDGVLSVIAPR 445
            V++ +  +GVL+V  P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140


>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
           similarity to SP|P23851 Ribosomal large subunit
           pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 478

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
 Frame = -3

Query: 401 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 231
           T  I+ YS  A      I    N A L  P+    PR   S PPF    LPG+NAG  G
Sbjct: 14  TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72


>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
           contains Pfam profile: PF04615 Utp14 protein
          Length = 822

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 17/54 (31%), Positives = 23/54 (42%)
 Frame = +2

Query: 257 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 418
           +EI     D Y   EG  EE+  ++    R     + L E C  E+VES    D
Sbjct: 48  DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101


>At3g49540.1 68416.m05414 expressed protein 
          Length = 166

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 17/49 (34%), Positives = 28/49 (57%)
 Frame = +2

Query: 374 EGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIAQTGPVRKEVK 520
           E    E+ E+++ +   ++V AP KV  AVE E+K   A+  PV+ E +
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAEAE 142


>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
           protein low similarity to fertility restorer [Petunia x
           hybrida] GI:22128587, post-transcriptional control of
           chloroplast gene expression CRP1 [Zea mays] GI:3289002;
           contains Pfam profile PF01535: PPR repeat
          Length = 991

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 15/48 (31%), Positives = 20/48 (41%)
 Frame = -3

Query: 422 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 279
           HHL  +   ++ P S P   S      +  H   SS      PRCS+P
Sbjct: 26  HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73


>At1g34180.1 68414.m04239 no apical meristem (NAM) family protein
           contains Pfam PF02365: No apical meristem (NAM) domain;
           similar to NAM-like protein GI:8809651 from (Arabidopsis
           thaliana)
          Length = 564

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 16/50 (32%), Positives = 24/50 (48%)
 Frame = +2

Query: 296 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 445
           +E    E  D  G   RQF     +P+G +AE ++S L ++     I PR
Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,012,760
Number of Sequences: 28952
Number of extensions: 339929
Number of successful extensions: 948
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 912
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 948
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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