Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0002
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 274 3e-74
At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 272 1e-73
At1g27660.1 68414.m03381 ethylene-responsive protein -related co... 28 6.8
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 28 6.8
At5g39390.1 68418.m04772 leucine-rich repeat family protein / pr... 27 9.0
At4g18240.1 68417.m02709 starch synthase-related protein contain... 27 9.0
At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein ... 27 9.0
At1g48840.1 68414.m05468 expressed protein contains Pfam profile... 27 9.0
At1g40129.1 68414.m04766 hypothetical protein 27 9.0
>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Arabidopsis thaliana}; strong similarity to SP|P13086
Succinyl-CoA ligase [GDP-forming] alpha-chain,
mitochondrial precursor {Rattus norvegicus}; contains
Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
domain
Length = 347
Score = 274 bits (673), Expect = 3e-74
Identities = 125/190 (65%), Positives = 147/190 (77%)
Frame = +2
Query: 131 LILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKA 310
+ + T+V+ QG TGK GTFH++QA++YGTK+V GV+PKK GTEHLG PVF +V EAKA
Sbjct: 52 VFVDKNTRVLCQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNSVAEAKA 111
Query: 311 GTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSRLVG 490
T A ASVIYVP P + LIVCITEG+PQHDMVRVKHAL Q+K+RL+G
Sbjct: 112 DTKANASVIYVPAPFAAAAIMEGIEAELDLIVCITEGIPQHDMVRVKHALNSQSKTRLIG 171
Query: 491 PNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGD 670
PNCPGII P +CKIGIMP +HK G IG+VSRSGTLTYEA QTT GLGQ+ CVGIGGD
Sbjct: 172 PNCPGIIKPGECKIGIMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGD 231
Query: 671 PFNGTDFIDC 700
PFNGT+F+DC
Sbjct: 232 PFNGTNFVDC 241
>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial, putative / succinyl-CoA
synthetase, alpha chain, putative / SCS-alpha, putative
similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
alpha-chain, mitochondrial precursor (EC 6.2.1.4)
(Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
{Dictyostelium discoideum}; contains Pfam profiles
PF00549: CoA-ligase, PF02629: CoA binding domain
Length = 341
Score = 272 bits (668), Expect = 1e-73
Identities = 126/190 (66%), Positives = 146/190 (76%)
Frame = +2
Query: 131 LILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKA 310
+ + T+VI QG TGK GTFH++QA++YGTK+V GV+PKK GTEHLG PVF TV EAKA
Sbjct: 47 VFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVAGVTPKKGGTEHLGLPVFNTVAEAKA 106
Query: 311 GTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCITEGVPQHDMVRVKHALLRQNKSRLVG 490
T A ASVIYVP P + LIVCITEG+PQHDMVRVK AL Q+K+RL+G
Sbjct: 107 ETKANASVIYVPAPFAAAAIMEGLAAELDLIVCITEGIPQHDMVRVKAALNSQSKTRLIG 166
Query: 491 PNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGD 670
PNCPGII P +CKIGIMP +HK G IG+VSRSGTLTYEA QTT GLGQ+ CVGIGGD
Sbjct: 167 PNCPGIIKPGECKIGIMPGYIHKPGKIGIVSRSGTLTYEAVFQTTAVGLGQSTCVGIGGD 226
Query: 671 PFNGTDFIDC 700
PFNGT+F+DC
Sbjct: 227 PFNGTNFVDC 236
>At1g27660.1 68414.m03381 ethylene-responsive protein -related
contains similarity to ethylene-inducible ER33 protein
[Lycopersicon esculentum] gi|5669656|gb|AAD46413
Length = 453
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = -1
Query: 337 NNRGSCSCACLCLFDCTKHRLTKMFCTSLLW*HSSNNFSTIVKGLLAVEGTLLSSETLNN 158
NN + + C+ L H L + L W H +++ +GLL ++ LSS T+++
Sbjct: 70 NNNNNNTSECMSLSSIHNHSLIQQQDFPLQWPHDQSSYQH-HEGLLKIKEE-LSSSTISD 127
Query: 157 H 155
H
Sbjct: 128 H 128
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +2
Query: 527 KIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGT 685
+ ++PA +H+R V + + + QTT+T + VG D +N T
Sbjct: 27 RFAVLPAFLHRRYATSVKLTAISAALKTVEQTTLTEDNRFSTVGSDSDEYNPT 79
>At5g39390.1 68418.m04772 leucine-rich repeat family protein /
protein kinase family protein contains Pfam PF00560:
Leucine Rich Repeat and PF00069: Protein kinase domain
Length = 502
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -1
Query: 235 SNNFSTIVKGLLAVEGTLLSSETLN 161
S NFS + KGLL +E L++ + LN
Sbjct: 217 SGNFSDVFKGLLGLEEKLVAVKVLN 241
>At4g18240.1 68417.m02709 starch synthase-related protein contains
similarity to starch synthase GI:4582783 from [Vigna
unguiculata]
Length = 1040
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +2
Query: 395 PLIVCITEGVPQHDMVRVKHALLR 466
PL+ CIT VPQ + ++HA+ R
Sbjct: 842 PLVGCITRLVPQKGVHLIRHAIYR 865
>At2g33640.1 68415.m04124 zinc finger (DHHC type) family protein
contains Pfam profile PF01529: DHHC zinc finger domain
Length = 565
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 370 NGCCSSRGRYINNRGSCSCACLCLFDCTK 284
+GCCS+ GR+I C CL + DC +
Sbjct: 109 SGCCSAIGRFI-------CGCLVIQDCRR 130
>At1g48840.1 68414.m05468 expressed protein contains Pfam profile:
PF04842 plant protein of unknown function (DUF639)
Length = 691
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = +2
Query: 143 SETKVIVQGFTGKQGTFHSQQALDYGTK---VVGGVSPKKAGTEHLGKPVFGTV 295
S ++V+ + K+GT HS A D ++ V G SPK + +G+ + G V
Sbjct: 402 SSSRVLDRTAKAKEGTLHSISASDMVSQLGLVFGATSPKSRSSLVVGEVMVGDV 455
>At1g40129.1 68414.m04766 hypothetical protein
Length = 567
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
Frame = -2
Query: 273 PRCSVPAFFGDTPPTTLVP-*SRACWLWK 190
PR FF D PP +P +R C LW+
Sbjct: 329 PRAECNIFFKDKPPDATLPFKTRGCTLWR 357
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,549,644
Number of Sequences: 28952
Number of extensions: 329839
Number of successful extensions: 800
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 800
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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