Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120056.Seq
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g50920.1 68414.m05725 GTP-binding protein-related similar to ... 128 5e-30
At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 118 5e-27
At1g80770.1 68414.m09476 expressed protein 61 8e-10
At1g51390.1 68414.m05782 nitrogen fixation NifU-like family prot... 32 0.34
At5g15440.1 68418.m01807 circadian clock coupling factor-related... 31 0.59
At3g56530.1 68416.m06286 no apical meristem (NAM) protein-relate... 31 0.59
At1g67770.1 68414.m07733 RNA-binding protein, putative similar t... 31 0.59
At4g00790.1 68417.m00109 expressed protein 30 1.4
At5g48340.1 68418.m05973 expressed protein 30 1.8
At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase... 29 2.4
At1g48740.1 68414.m05454 expressed protein 29 2.4
At1g49920.1 68414.m05598 zinc finger protein-related weak simila... 29 3.2
At1g11520.1 68414.m01323 pliceosome associated protein-related c... 29 3.2
At1g67230.1 68414.m07652 expressed protein 26 3.5
At5g21080.1 68418.m02510 expressed protein predicted proteins - ... 28 5.5
At5g53970.1 68418.m06714 aminotransferase, putative similar to n... 27 9.6
>At1g50920.1 68414.m05725 GTP-binding protein-related similar to
GTP-binding protein SP:Q99ME9 from [Mus musculus]
Length = 671
Score = 128 bits (308), Expect = 5e-30
Identities = 56/83 (67%), Positives = 70/83 (84%)
Frame = +2
Query: 2 GHTDYKYLRWQVIDTPGILDHPLEERNVIEMQAVTALAHLRAAILYFIDPSEQCGHSIED 181
GHTDYKYLR+QVIDTPGILD P E+RN+IEM ++TALAHLRAA+L+F+D S CG++I
Sbjct: 208 GHTDYKYLRYQVIDTPGILDRPFEDRNIIEMCSITALAHLRAAVLFFLDISGSCGYTIAQ 267
Query: 182 QISLFESIKPLFTSKPLIVVLNK 250
Q +LF SIK LF +KPL++V NK
Sbjct: 268 QAALFHSIKSLFMNKPLVIVCNK 290
Score = 53.2 bits (122), Expect = 2e-07
Identities = 24/43 (55%), Positives = 33/43 (76%)
Frame = +1
Query: 376 VMRMSTVTEEGVQEVKIEACERLLGHRVTEKMRTKKVDGILNR 504
+++MST+T+EGV VK ACERLL RV KM++KK++ LNR
Sbjct: 327 LLKMSTLTDEGVMSVKNAACERLLDQRVEAKMKSKKINDHLNR 369
Score = 45.6 bits (103), Expect = 3e-05
Identities = 30/80 (37%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = +3
Query: 492 HLEXXXXXXXXXRDDKPRPPVLPPSVLAKKEKQAEKENRKRKLERDIEVELGDD--YVLD 665
HL RD R P +P VL K K+A KRK E+D+E E G Y
Sbjct: 366 HLNRFHVAIPKPRDSIERLPCIPQVVLEAKAKEAAAME-KRKTEKDLEEENGGAGVYSAS 424
Query: 666 LKKNYTEIPEDERYDVIPEI 725
LKKNY ++ + D++PEI
Sbjct: 425 LKKNYILQHDEWKEDIMPEI 444
>At1g10300.1 68414.m01160 GTP-binding protein-related contains
similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
from [Homo sapiens];
Length = 687
Score = 118 bits (283), Expect = 5e-27
Identities = 50/83 (60%), Positives = 69/83 (83%)
Frame = +2
Query: 2 GHTDYKYLRWQVIDTPGILDHPLEERNVIEMQAVTALAHLRAAILYFIDPSEQCGHSIED 181
GHTDYK LR+QVIDTPG+LD +E+RN+IE+ ++TALAH+RAA+L+F+D S CG++I
Sbjct: 237 GHTDYKCLRYQVIDTPGLLDREIEDRNIIELCSITALAHIRAAVLFFLDISGSCGYTIAQ 296
Query: 182 QISLFESIKPLFTSKPLIVVLNK 250
Q SLF +IK +F +KPL++V NK
Sbjct: 297 QASLFHNIKSVFKNKPLVIVCNK 319
Score = 45.6 bits (103), Expect = 3e-05
Identities = 32/85 (37%), Positives = 40/85 (47%), Gaps = 2/85 (2%)
Frame = +3
Query: 477 KEGGRHLEXXXXXXXXXRDDKPRPPVLPPSVLAKKEKQAEKENRKRKLERDIEVELGDD- 653
K+ G HL RDD+ R P +P Q E KRK E+D+E E G
Sbjct: 383 KKIGDHLNRFHVAMPKSRDDQERLPCIP---------QVAGEKEKRKTEKDLEDENGGAG 433
Query: 654 -YVLDLKKNYTEIPEDERYDVIPEI 725
Y LKKNY E+ + D+IPEI
Sbjct: 434 VYSASLKKNYILAKEEWKDDIIPEI 458
Score = 35.5 bits (78), Expect = 0.036
Identities = 20/43 (46%), Positives = 26/43 (60%)
Frame = +1
Query: 376 VMRMSTVTEEGVQEVKIEACERLLGHRVTEKMRTKKVDGILNR 504
++ MST+TEEGV RLL RV KM++KK+ LNR
Sbjct: 356 ILEMSTLTEEGVMS-------RLLDQRVAAKMKSKKIGDHLNR 391
>At1g80770.1 68414.m09476 expressed protein
Length = 451
Score = 60.9 bits (141), Expect = 8e-10
Identities = 29/72 (40%), Positives = 41/72 (56%)
Frame = +2
Query: 2 GHTDYKYLRWQVIDTPGILDHPLEERNVIEMQAVTALAHLRAAILYFIDPSEQCGHSIED 181
GH Y R+QV DTPG+L E+RN +E + L HL A+LY D + +CG S D
Sbjct: 293 GHIVLNYQRFQVTDTPGLLRRCDEDRNNLEKLTLAVLTHLPTAVLYVHDLTGECGTSPSD 352
Query: 182 QISLFESIKPLF 217
Q +++ +K F
Sbjct: 353 QFQIYKEMKERF 364
>At1g51390.1 68414.m05782 nitrogen fixation NifU-like family protein
simikar to HIRA-interacting protein 5 (NIFU FAMILY)
(SP:Q9QZ23) {Mus musculus}; contains Pfam profile:
PF01106 NifU-like domain
Length = 275
Score = 32.3 bits (70), Expect = 0.34
Identities = 22/60 (36%), Positives = 35/60 (58%), Gaps = 3/60 (5%)
Frame = -3
Query: 461 VTRCPSSLSQASILTSCTPSSVTVDIRITGTTDRSEFESALTRFPL---EHVSVRSSSNC 291
+TR +SLS+ + +C+PSS ++ I + RS F SA T FP+ E ++ +S NC
Sbjct: 4 LTRLLNSLSRHTASVTCSPSSSSLLI-----SRRSLFISAATHFPVNFSESITTNASRNC 58
>At5g15440.1 68418.m01807 circadian clock coupling factor-related
similar to circadian clock coupling factor ZGT
[Nicotiana tabacum] GI:14210079
Length = 293
Score = 31.5 bits (68), Expect = 0.59
Identities = 21/79 (26%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = +2
Query: 122 RAAILYFIDPSEQCGHSIEDQISLFESI-KPLFTSKPLIVVLNKMV-WSNLKSCL*VRNS 295
R +LY DP E E + LF I K TSK V++NK V + + C +
Sbjct: 136 RTDVLYVCDPCEHLDQGEEGDVGLFRGIFKSFPTSKVRKVIINKAVPFHPSEVCPYCKAK 195
Query: 296 CWTI*PKRALKEILSMRIQ 352
W++ + + + +R++
Sbjct: 196 LWSMLQAKIIPQSACIRLE 214
>At3g56530.1 68416.m06286 no apical meristem (NAM) protein-related
contains Pfam PF02365 : No apical meristem (NAM)
protein; similar to NAC domain protein NAC2
(GI:21554255) {Arabidopsis thaliana}
Length = 319
Score = 31.5 bits (68), Expect = 0.59
Identities = 19/51 (37%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +3
Query: 573 AKKEKQAEKENRKRKLERDIE-VELGDDYVLDLKKNYTEIPEDERYDVIPE 722
AKK+K E EN K K E D+E ++L L L++ + +I +Y ++PE
Sbjct: 205 AKKKKAEEAENEKLKKEEDVEQLDLNQPDQLQLQQPH-DIVYQPQYCLLPE 254
>At1g67770.1 68414.m07733 RNA-binding protein, putative similar to
terminal ear1 gb|AAC39463.1
Length = 527
Score = 31.5 bits (68), Expect = 0.59
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +3
Query: 546 PPVLPPSVLAKKEKQAEKENRKRKLERDIEVELGDDYVL 662
PP PPS + K + K+ +K+K ++ ++ LGD Y +
Sbjct: 283 PPPPPPSRMVKSDILMYKQQQKKKKKKYVKKNLGDPYFM 321
>At4g00790.1 68417.m00109 expressed protein
Length = 435
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = -1
Query: 175 NTMATLLRRIYEV*YGSSEMSQSCDRLHFYNIAFFQWMIQDSRGVYHLPSQI 20
N + LL+ + E + + +SQ C++ HFY + + +I D R V L S +
Sbjct: 136 NQLLNLLKAVPETDWDADYVSQLCEKAHFYQVCGYIHII-DRRYVAALDSYV 186
>At5g48340.1 68418.m05973 expressed protein
Length = 510
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/37 (43%), Positives = 25/37 (67%), Gaps = 2/37 (5%)
Frame = +3
Query: 564 SVLAKKEKQAEKENRKRKLER--DIEVELGDDYVLDL 668
S ++KK + EK+ RK+K +R D + + GDD +LDL
Sbjct: 430 SRVSKKRHKREKKKRKKKKKRAFDDDDDRGDDELLDL 466
>At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase,
putative / 1-deoxyxylulose-5-phosphate synthase,
putative / DXP-synthase, putative (DEF) (CLA1) identical
to SP|Q38854 Probable 1-deoxy-D-xylulose 5-phosphate
synthase, chloroplast precursor (EC 4.1.3.37)
(1-deoxyxylulose-5-phosphate synthase) (DXP synthase)
(DXPS). [Mouse-ear cress] {Arabidopsis thaliana}, DEF
(deficient in photosynthesis) protein [Arabidopsis
thaliana] GI:1399261
Length = 717
Score = 29.5 bits (63), Expect = 2.4
Identities = 11/35 (31%), Positives = 24/35 (68%)
Frame = +2
Query: 134 LYFIDPSEQCGHSIEDQISLFESIKPLFTSKPLIV 238
LY+I P + GH+I+D +++ + +K T+ P+++
Sbjct: 326 LYYIGPVD--GHNIDDLVAILKEVKSTRTTGPVLI 358
>At1g48740.1 68414.m05454 expressed protein
Length = 393
Score = 29.5 bits (63), Expect = 2.4
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -3
Query: 554 HGRARLVVARGRHRHGSRFKMPS 486
H + ++ RGRHRHG R PS
Sbjct: 311 HSPGQAILHRGRHRHGPRANTPS 333
>At1g49920.1 68414.m05598 zinc finger protein-related weak
similarity to mudrA [Zea mays] GI:540581, MURAZC [Zea
mays] GI:1857256; contains Pfam profiles PF03108: MuDR
family transposase, PF04434: SWIM zinc finger
Length = 785
Score = 29.1 bits (62), Expect = 3.2
Identities = 19/56 (33%), Positives = 31/56 (55%), Gaps = 4/56 (7%)
Frame = +3
Query: 546 PPVL---PPSVLAK-KEKQAEKENRKRKLERDIEVELGDDYVLDLKKNYTEIPEDE 701
PPV+ PP V K KEK E ++ + + D + E DD V D +++ ++ +DE
Sbjct: 726 PPVIEPPPPKVSGKGKEKDTEDDHLEEDEDGDDDEEEEDDDVDDDEEDDDDVDDDE 781
>At1g11520.1 68414.m01323 pliceosome associated protein-related
contains similarity to spliceosome associated protein
SAP 145 GI:1173904 from [Homo sapiens]
Length = 196
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +3
Query: 564 SVLAKKEKQAEKENRKRKLERDIEVELGDDYVLDLKKNYTEIP 692
SVL KK ++ ++ R+RK ++ + D +D+ K+ + P
Sbjct: 55 SVLPKKSREIDRRRRRRKRKKKNKASQADVDAMDVSKSLSSTP 97
>At1g67230.1 68414.m07652 expressed protein
Length = 1132
Score = 25.8 bits (54), Expect(2) = 3.5
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 576 KKEKQAEKENRKRKLE 623
KKEKQA EN +R+LE
Sbjct: 561 KKEKQAANENMERELE 576
Score = 21.4 bits (43), Expect(2) = 3.5
Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = +3
Query: 600 ENRKRKLERDIEVELGD-DYVLDLKKNYTE 686
E RKRKLE D++ L + + L KK E
Sbjct: 612 EMRKRKLESDMQTILEEKERELQAKKKLFE 641
>At5g21080.1 68418.m02510 expressed protein predicted proteins -
Arabidopsis thaliana; expression supported by MPSS
Length = 980
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -3
Query: 473 RIFSVTRCPSSLSQASILTSCTPSSV 396
RIFSV PSS+S +S+L S P+ +
Sbjct: 481 RIFSVVLVPSSVSPSSVLNSRRPADM 506
>At5g53970.1 68418.m06714 aminotransferase, putative similar to
nicotianamine aminotransferase from Hordeum vulgare
[GI:6498122, GI:6469087]; contains Pfam profile PF00155
aminotransferase, classes I and II
Length = 414
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 2/45 (4%)
Frame = +2
Query: 86 IEMQAVTALAHLRAAILYFIDPSEQCG--HSIEDQISLFESIKPL 214
I++ AV ALA L I+P CG +S + + + ES K L
Sbjct: 159 IDLDAVEALADENTVALVVINPGNPCGNVYSYQHLMKIAESAKKL 203
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,816,664
Number of Sequences: 28952
Number of extensions: 252486
Number of successful extensions: 1006
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 948
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 988
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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