Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV120053.Seq
(771 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 68 6e-12
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 65 4e-11
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 64 1e-10
At3g17205.1 68416.m02196 HECT-domain-containing protein / ubiqui... 56 3e-08
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 50 2e-06
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 43 2e-04
At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) ... 30 1.5
At2g38240.1 68415.m04696 oxidoreductase, 2OG-Fe(II) oxygenase fa... 30 1.9
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 29 2.6
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 68.1 bits (159), Expect = 6e-12
Identities = 35/83 (42%), Positives = 53/83 (63%), Gaps = 4/83 (4%)
Frame = +1
Query: 16 EYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI--KIRIQPV-- 183
EY NG+ ETD+QI WFW+ ++ E+++ L F T + VP++G R + K+ I +
Sbjct: 770 EY-NGFKETDRQIDWFWKILKKMTEEEQRSILFFWTSNKFVPVEGFRGLSSKLYIYRLYE 828
Query: 184 ADDRFFPVAHTCFNLLDLPRYKT 252
A+DR P++HTCF L +PRY T
Sbjct: 829 ANDR-LPLSHTCFYRLCIPRYPT 850
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 65.3 bits (152), Expect = 4e-11
Identities = 34/90 (37%), Positives = 50/90 (55%), Gaps = 8/90 (8%)
Frame = +1
Query: 10 NCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI-------KI 168
N EY + Y+ IRWFWE ED +FL F+TG+ +VP++G + + ++
Sbjct: 3549 NTEYTS-YTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRL 3607
Query: 169 RI-QPVADDRFFPVAHTCFNLLDLPRYKTK 255
+I + P AHTCFN LDLP Y++K
Sbjct: 3608 QIHKAYGSPERLPSAHTCFNQLDLPEYQSK 3637
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 64.1 bits (149), Expect = 1e-10
Identities = 35/91 (38%), Positives = 51/91 (56%), Gaps = 9/91 (9%)
Frame = +1
Query: 10 NCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDI-------KI 168
N EY + Y+ I WFWE ED +FL F+TG+ +VP++G + + ++
Sbjct: 3782 NTEYTS-YTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRL 3840
Query: 169 RIQPV--ADDRFFPVAHTCFNLLDLPRYKTK 255
+I A +R P AHTCFN LDLP Y++K
Sbjct: 3841 QIHKAYGAPER-LPSAHTCFNQLDLPEYQSK 3870
>At3g17205.1 68416.m02196 HECT-domain-containing protein /
ubiquitin-transferase family protein weak similarity to
ubiquitin-protein ligase 2 [Arabidopsis thaliana]
GI:7108523; contains Pfam profile PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 56.0 bits (129), Expect = 3e-08
Identities = 35/93 (37%), Positives = 46/93 (49%), Gaps = 9/93 (9%)
Frame = +1
Query: 4 QNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIK--IRIQ 177
+NN Y GY I FWE E++KKFL F+TG R P+ G + ++ IQ
Sbjct: 762 RNNTNYAGGYHAGHYVIDMFWEVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQ 821
Query: 178 PVAD-------DRFFPVAHTCFNLLDLPRYKTK 255
A DR P + TC NLL LP Y++K
Sbjct: 822 RAAGSASNESVDR-LPTSATCMNLLKLPPYQSK 853
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 49.6 bits (113), Expect = 2e-06
Identities = 25/89 (28%), Positives = 45/89 (50%), Gaps = 6/89 (6%)
Frame = +1
Query: 7 NNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIKIRIQPV- 183
++ ++ +GY+ + + + + HE E ++ FL F+TGS R+P G+ + ++ V
Sbjct: 1393 DHIKFDHGYTSSSPPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVR 1452
Query: 184 -----ADDRFFPVAHTCFNLLDLPRYKTK 255
+ D P TC N L LP Y +K
Sbjct: 1453 KHGSDSSDTDLPSVMTCANYLKLPPYSSK 1481
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains Pfam
profiles PF00632: HECT-domain (ubiquitin-transferase),
PF00612: IQ calmodulin-binding motif
Length = 1142
Score = 43.2 bits (97), Expect = 2e-04
Identities = 28/96 (29%), Positives = 43/96 (44%), Gaps = 14/96 (14%)
Frame = +1
Query: 4 QNNCEYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIK--IRIQ 177
+ N +Y GYS++ + I+ FWE ++ L F+T R P+ G + ++ I
Sbjct: 1025 RRNTKYTGGYSDSSRTIKIFWEVMKGFEPSERCLLLKFVTSCSRAPLLGFKYLQPTFIIH 1084
Query: 178 PVA------------DDRFFPVAHTCFNLLDLPRYK 249
V+ D P A TC+N L LP YK
Sbjct: 1085 KVSCDTSLWAAIGGQDVERLPSASTCYNTLKLPTYK 1120
>At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21)
identical to ubiquitin-specific protease 21 GI:11993482
[Arabidopsis thaliana]
Length = 732
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -3
Query: 493 KPPVYSKRQGTFEVDPARLIKKESDIDYRFDRPM 392
KP + KRQGTF++ A L K+ + + RP+
Sbjct: 643 KPNSHQKRQGTFQIQRAHLQTKKQEESRKTKRPL 676
>At2g38240.1 68415.m04696 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to flavonol synthase [Citrus
unshiu][gi:4126403], leucoanthocyanidin dioxygenase
[Daucus carota][gi:5924383]; contains PF03171 2OG-Fe(II)
oxygenase superfamily domain
Length = 353
Score = 29.9 bits (64), Expect = 1.9
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = +1
Query: 46 QQIRWFWETFHELPLEDKKKF 108
+++R W F ELPLE+K+K+
Sbjct: 89 ERVRGAWREFFELPLEEKRKY 109
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 29.5 bits (63), Expect = 2.6
Identities = 27/99 (27%), Positives = 41/99 (41%), Gaps = 19/99 (19%)
Frame = +1
Query: 16 EYKNGYSETDQQIRWFWETFHELPLEDKKKFLLFLTGSDRVPIQGMRDIKIRIQPV---- 183
++ +GY+ I E EL + ++ F F+TG+ R+P G+ + ++ V
Sbjct: 1770 KFDHGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHS 1829
Query: 184 ---------------ADDRFFPVAHTCFNLLDLPRYKTK 255
ADD P TC N L LP Y TK
Sbjct: 1830 STSSAAANGAGASETADDD-LPSVMTCANYLKLPPYSTK 1867
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,600,381
Number of Sequences: 28952
Number of extensions: 315124
Number of successful extensions: 662
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 657
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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