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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060082.seq
         (675 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g75210.1 68414.m08737 5' nucleotidase family protein contains...    85   5e-17
At2g23890.1 68415.m02853 5' nucleotidase family protein contains...    83   2e-16
At5g48960.1 68418.m06057 5' nucleotidase family protein low simi...    62   2e-10
At3g13270.1 68416.m01670 hypothetical protein contains similarit...    28   5.0  
At1g02110.1 68414.m00137 proline-rich family protein contains pr...    28   6.6  
At3g42500.1 68416.m04407 hypothetical protein includes At2g10370...    27   8.7  
At1g44860.1 68414.m05139 hypothetical protein includes At2g10370...    27   8.7  

>At1g75210.1 68414.m08737 5' nucleotidase family protein contains
           Pfam profile: PF05761 5' nucleotidase family
          Length = 642

 Score = 84.6 bits (200), Expect = 5e-17
 Identities = 42/92 (45%), Positives = 62/92 (67%), Gaps = 1/92 (1%)
 Frame = +3

Query: 237 QNFR*QSLHLENVKFYGFDMDYTLAEYKSPQYETLGFNLTKERLVVK-GYPREILEFEYD 413
           Q F  +SL+++N+   GFDMDYTLA+YKS  +E+L ++ T  +LV   GYP E+LE+ +D
Sbjct: 148 QIFCNRSLNMKNIIAVGFDMDYTLAQYKSETFESLAYDGTVRKLVYDLGYPNELLEWTFD 207

Query: 414 PSFPVRGLWFDTLYGNLLKVDAYGNILVCVHG 509
            ++ VRGL  D   GN+LK+D +  + V  HG
Sbjct: 208 WNYMVRGLVLDKKRGNILKMDRHKYVKVAYHG 239



 Score = 32.7 bits (71), Expect = 0.23
 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
 Frame = +2

Query: 509 FEFLKHSQVYELYPNKFL--TLDESRVYVLNTLFNLPETYLIACLIDFFTN 655
           F  L      E+Y +  +  + DE    +++TLF+L E YL A L+DF  N
Sbjct: 240 FRELSKEDKVEIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDN 290


>At2g23890.1 68415.m02853 5' nucleotidase family protein contains
           Pfam PF05761: 5' nucleotidase family; similar to
           Cytosolic purine 5'-nucleotidase (EC 3.1.3.5)
           (5'-nucleotidase cytosolic II) (Swiss-Prot:P49902) [Homo
           sapiens]
          Length = 553

 Score = 83.0 bits (196), Expect = 2e-16
 Identities = 38/81 (46%), Positives = 59/81 (72%), Gaps = 1/81 (1%)
 Frame = +3

Query: 252 QSLHLENVKFYGFDMDYTLAEYKSPQYETLGFNLTKERLVVK-GYPREILEFEYDPSFPV 428
           ++L L+N++ YGFD DYTLA Y S   ++L ++L K+ +V +  YP    +FEYDP+FP+
Sbjct: 103 KNLRLDNIQVYGFDYDYTLAHYSS-HLQSLIYDLAKKHMVNEFRYPDVCTQFEYDPTFPI 161

Query: 429 RGLWFDTLYGNLLKVDAYGNI 491
           RGL++D L G L+K+D +G+I
Sbjct: 162 RGLYYDKLKGCLMKLDFFGSI 182


>At5g48960.1 68418.m06057 5' nucleotidase family protein low
           similarity to SP|P49902 Cytosolic purine 5'-nucleotidase
           (EC 3.1.3.5) (5'-nucleotidase cytosolic II) {Homo
           sapiens}; contains Pfam profile PF05761: 5' nucleotidase
           family
          Length = 642

 Score = 62.5 bits (145), Expect = 2e-10
 Identities = 31/86 (36%), Positives = 47/86 (54%)
 Frame = +3

Query: 252 QSLHLENVKFYGFDMDYTLAEYKSPQYETLGFNLTKERLVVKGYPREILEFEYDPSFPVR 431
           ++L+L ++   G+DMDYTL  Y    +E   ++   E L   G+P + L F  DP   +R
Sbjct: 169 RTLNLRSISAIGYDMDYTLMHYNVMAWEGKAYDYCMENLKSMGFPVDGLAF--DPELVIR 226

Query: 432 GLWFDTLYGNLLKVDAYGNILVCVHG 509
           GL  D   GNL+K D +G +   +HG
Sbjct: 227 GLMIDKEKGNLVKADRFGYVKRAMHG 252


>At3g13270.1 68416.m01670 hypothetical protein contains similarity
           to replication protein A1
          Length = 570

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = -3

Query: 664 WGEIREEIYEARNQIGFRQVKQSVQNVNPRFI*SQEFI 551
           WG   +++Y   N+ G R V   ++N++  F  SQ F+
Sbjct: 177 WGTFAQQVYRGCNEAGARPVVCVLRNLSNSFDASQVFV 214


>At1g02110.1 68414.m00137 proline-rich family protein contains
           proline-rich domain, INTERPRO:IPR000694
          Length = 679

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 13/25 (52%), Positives = 17/25 (68%)
 Frame = -1

Query: 114 IVYFQKIYSTSEHALFLLSTVPLAN 40
           I+ FQ+ +  S HA F LS VPL+N
Sbjct: 486 IIKFQREFICSLHAWFKLSLVPLSN 510


>At3g42500.1 68416.m04407 hypothetical protein includes At2g10370,
           At5g36050, At1g35090, At1g44860, At4g19300, At1g42400,
           At3g43040, At3g42500
          Length = 140

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 7/21 (33%), Positives = 13/21 (61%)
 Frame = +2

Query: 488 YFSVCTWFEFLKHSQVYELYP 550
           Y+  C WF+ + H+ +  +YP
Sbjct: 105 YYPGCNWFKIVSHAPISTMYP 125


>At1g44860.1 68414.m05139 hypothetical protein includes At2g10370,
           At5g36050, At1g35090, At1g44860, At4g19300, At1g42400,
           At3g43040, At3g42500
          Length = 244

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 7/21 (33%), Positives = 13/21 (61%)
 Frame = +2

Query: 488 YFSVCTWFEFLKHSQVYELYP 550
           Y+  C WF+ + H+ +  +YP
Sbjct: 209 YYPGCNWFKIVSHAPISTMYP 229


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,053,893
Number of Sequences: 28952
Number of extensions: 285638
Number of successful extensions: 739
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 736
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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