Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060057.seq
(691 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16340.1 68416.m02066 ABC transporter family protein similar ... 34 0.077
At3g11720.1 68416.m01437 expressed protein 30 1.7
At2g22795.1 68415.m02704 expressed protein 29 2.9
At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,... 29 2.9
At5g62370.1 68418.m07828 pentatricopeptide (PPR) repeat-containi... 29 3.8
At5g50260.1 68418.m06224 cysteine proteinase, putative similar t... 29 3.8
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 29 3.8
At4g16280.2 68417.m02470 flowering time control protein / FCA ga... 29 3.8
At4g16280.1 68417.m02469 flowering time control protein / FCA ga... 29 3.8
At3g63070.1 68416.m07084 PWWP domain-containing protein putative... 29 3.8
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 5.1
At2g31830.1 68415.m03887 endonuclease/exonuclease/phosphatase fa... 28 6.7
At1g45191.2 68414.m05184 glycosyl hydrolase family 1 protein Sin... 28 6.7
At1g36390.2 68414.m04521 co-chaperone grpE family protein simila... 28 6.7
At1g36390.1 68414.m04520 co-chaperone grpE family protein simila... 28 6.7
At3g58260.1 68416.m06495 meprin and TRAF homology domain-contain... 27 8.9
At3g22160.1 68416.m02797 VQ motif-containing protein contains PF... 27 8.9
At1g74690.1 68414.m08650 calmodulin-binding family protein low s... 27 8.9
>At3g16340.1 68416.m02066 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]; contains Pfam profile: PF00005 ABC
transporter
Length = 1416
Score = 34.3 bits (75), Expect = 0.077
Identities = 18/57 (31%), Positives = 29/57 (50%)
Frame = +3
Query: 309 KWMKQAKVIXGKKITTTDTAIHFKKLKSVKLGIDDYQKFLDDLAKNKKVELDEIKKK 479
KW K+ ++ TT H + KLG+DD QKF+D + K + + ++ KK
Sbjct: 41 KWAALEKLPTFARLRTTIIHPHEDLVDVTKLGVDDRQKFIDSIFKVTEEDNEKFLKK 97
>At3g11720.1 68416.m01437 expressed protein
Length = 542
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = +1
Query: 124 VEQVTQE--VKDVKLENGNAPGASNGTSSKSEDNALSFKEAF 243
V ++ +E V D+K EN ++P +S+ +SS SED + K F
Sbjct: 348 VPEIEEEECVDDLKEENKSSPSSSSSSSSSSEDEDKNGKTRF 389
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/38 (34%), Positives = 23/38 (60%)
Frame = +1
Query: 97 TEAQNTDAAVEQVTQEVKDVKLENGNAPGASNGTSSKS 210
T+ Q+ ++ + QEVKDV+ + P + NG S++S
Sbjct: 693 TQEQSDSSSDTNLPQEVKDVRTDLETLPDSGNGGSNES 730
>At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase SP:P45582 from
[Asparagus officinalis]
Length = 815
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +2
Query: 56 HHTNTLNTYKEKCLLKHRIRMPLWSK 133
H N+L ++ CL K R +P+WSK
Sbjct: 769 HSPNSLAVVQKACLKKSRCSVPVWSK 794
>At5g62370.1 68418.m07828 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 982
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 5/38 (13%)
Frame = +3
Query: 432 DLAKNKKVELDE-----IKKKLTTCGQPGITHTLQNHR 530
D A + +ELD + +KLT GQPG+ T N R
Sbjct: 84 DFAVDNGIELDSSCYGALIRKLTEMGQPGVAETFYNQR 121
>At5g50260.1 68418.m06224 cysteine proteinase, putative similar to
cysteine endopeptidase precursor CysEP GI:2944446 from
[Ricinus communis]
Length = 361
Score = 28.7 bits (61), Expect = 3.8
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +2
Query: 257 IWRSQVRWKSHHALAKRQMDETSQSYXWKENNNNGH 364
+W RW+SHH +A+ ++ + +K N + H
Sbjct: 34 LWELYERWRSHHTVARSLEEKAKRFNVFKHNVKHIH 69
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1
Query: 97 TEAQNTDAAVEQVTQEVKDVKL 162
TE N D +E V Q++KD KL
Sbjct: 265 TEESNVDEVIESVKQQIKDAKL 286
>At4g16280.2 68417.m02470 flowering time control protein / FCA gamma
(FCA) identical to SP|O04425 Flowering time control
protein FCA {Arabidopsis thaliana}; four alternative
splice variants, one splicing isoform contains a
non-consensus CA donor splice site, based on cDNA:
gi:2204090
Length = 747
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/36 (30%), Positives = 22/36 (61%)
Frame = +1
Query: 106 QNTDAAVEQVTQEVKDVKLENGNAPGASNGTSSKSE 213
Q++ A+ Q+ Q+V+ ++ N N P + NG + K +
Sbjct: 523 QSSQQAISQLQQQVQSMQQPNQNLPLSQNGRAGKQQ 558
>At4g16280.1 68417.m02469 flowering time control protein / FCA gamma
(FCA) identical to SP|O04425 Flowering time control
protein FCA {Arabidopsis thaliana}; four alternative
splice variants, one splicing isoform contains a
non-consensus CA donor splice site, based on cDNA:
gi:2204090
Length = 505
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/36 (30%), Positives = 22/36 (61%)
Frame = +1
Query: 106 QNTDAAVEQVTQEVKDVKLENGNAPGASNGTSSKSE 213
Q++ A+ Q+ Q+V+ ++ N N P + NG + K +
Sbjct: 281 QSSQQAISQLQQQVQSMQQPNQNLPLSQNGRAGKQQ 316
>At3g63070.1 68416.m07084 PWWP domain-containing protein putative
transcription factor HUA2, Arabidopsis thaliana,
EMBL:AF116556
Length = 1347
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/64 (29%), Positives = 26/64 (40%)
Frame = +1
Query: 100 EAQNTDAAVEQVTQEVKDVKLENGNAPGASNGTSSKSEDNALSFKEAFKAFSNLEIPSPM 279
E + D + Q V ENG G+ + TS+ E N+ +PS M
Sbjct: 436 ERSSKDTLLSAALQTSPSVNHENGI--GSGHDTSAAEEFNSFELSAKLSGVMVDVVPSHM 493
Query: 280 EKPS 291
EKPS
Sbjct: 494 EKPS 497
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 28.3 bits (60), Expect = 5.1
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = +1
Query: 64 KYIKHL*RKMSTEAQNTDAAVEQVTQEVKDVKL 162
K IK + TE N D E V Q+VKD KL
Sbjct: 254 KEIKAVLTSQVTEEINVDEVTEMVKQQVKDAKL 286
>At2g31830.1 68415.m03887 endonuclease/exonuclease/phosphatase
family protein belongs to Pfam:PF03372:
Endonuclease/Exonuclease/phosphatase family; contains 4
WD-40 repeats (PF00400);similar to SP:Q01968 Inositol
polyphosphate 5-phosphatase OCRL-1 (EC 3.1.3.-)(Lowe's
oculocerebrorenal syndrome protein) (SP:Q01968) [Homo
sapiens]
Length = 1144
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = +2
Query: 188 PMERPVKARITPYLSRKPSRRFQIWRSQVRWKSHHALAKRQMDETSQS 331
P ER A + P P R+ + Q K HH + K +DE S+S
Sbjct: 8 PDEREALASLVP-AHPLPPRKTHSYVEQCEQKPHHPIRKYSLDEGSRS 54
>At1g45191.2 68414.m05184 glycosyl hydrolase family 1 protein Since
this genomic sequence region is unfinished, the
annotated gene may be missing a stop codon or start
codon
Length = 487
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = +3
Query: 339 GKKITTTDTAIHFKKLKSVKLGIDDYQKFLDDL 437
G+K + DT +H +K+ + + D Y K+ +D+
Sbjct: 57 GRKPSVWDTFLHCRKMDNGDIACDGYHKYKEDV 89
>At1g36390.2 68414.m04521 co-chaperone grpE family protein similar
to co-chaperone CGE1 precursor isoform b [Chlamydomonas
reinhardtii] GI:15384279; contains Pfam profile PF01025:
co-chaperone GrpE
Length = 279
Score = 27.9 bits (59), Expect = 6.7
Identities = 18/58 (31%), Positives = 31/58 (53%)
Frame = +2
Query: 215 ITPYLSRKPSRRFQIWRSQVRWKSHHALAKRQMDETSQSYXWKENNNNGHGYSLQKTQ 388
I+P +S KPSR+F I+ + S A +K+Q D + +K+ NG S+ + +
Sbjct: 42 ISPLIS-KPSRKFPIFSAHQTNNSEEANSKQQADVKTLIRSYKQALLNGDETSVTEIE 98
>At1g36390.1 68414.m04520 co-chaperone grpE family protein similar
to co-chaperone CGE1 precursor isoform b [Chlamydomonas
reinhardtii] GI:15384279; contains Pfam profile PF01025:
co-chaperone GrpE
Length = 279
Score = 27.9 bits (59), Expect = 6.7
Identities = 18/58 (31%), Positives = 31/58 (53%)
Frame = +2
Query: 215 ITPYLSRKPSRRFQIWRSQVRWKSHHALAKRQMDETSQSYXWKENNNNGHGYSLQKTQ 388
I+P +S KPSR+F I+ + S A +K+Q D + +K+ NG S+ + +
Sbjct: 42 ISPLIS-KPSRKFPIFSAHQTNNSEEANSKQQADVKTLIRSYKQALLNGDETSVTEIE 98
>At3g58260.1 68416.m06495 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 321
Score = 27.5 bits (58), Expect = 8.9
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +3
Query: 384 LKSVKLGIDDYQKFLDDLAKNKKVELDEIK 473
+KSV +D +K LD+L + KK E D+I+
Sbjct: 226 MKSVGFKLDWLEKKLDELFEKKKEEADKIR 255
>At3g22160.1 68416.m02797 VQ motif-containing protein contains
PF05678: VQ motif
Length = 192
Score = 27.5 bits (58), Expect = 8.9
Identities = 20/68 (29%), Positives = 36/68 (52%)
Frame = +1
Query: 91 MSTEAQNTDAAVEQVTQEVKDVKLENGNAPGASNGTSSKSEDNALSFKEAFKAFSNLEIP 270
++T+ N A V+Q T + +GN A + TSS S+ +A S ++A + N +
Sbjct: 69 LNTDTSNFRAMVQQYTGGPSAMAFGSGNTTSAFSLTSS-SDPSAGSSQQAPWQY-NFQPH 126
Query: 271 SPMEKPSR 294
+P++ P R
Sbjct: 127 APLQPPQR 134
>At1g74690.1 68414.m08650 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 587
Score = 27.5 bits (58), Expect = 8.9
Identities = 17/93 (18%), Positives = 41/93 (44%), Gaps = 2/93 (2%)
Frame = +3
Query: 270 KSDGKAITLSQSDKWMKQAKVIXGKKI--TTTDTAIHFKKLKSVKLGIDDYQKFLDDLAK 443
K + + ++S K K + + ++ T+ F+K K + Q +
Sbjct: 268 KPQNRLLVEAESAKPKKSVRKVPASNFESSSVQTSFEFEKPKRSFRKVSS-QSIEPPAVE 326
Query: 444 NKKVELDEIKKKLTTCGQPGITHTLQNHRQPQR 542
+ ++EL+++K+ L P + ++Q R P++
Sbjct: 327 DPQIELEKVKRSLRKVHNPVVESSIQPQRSPRK 359
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,873,412
Number of Sequences: 28952
Number of extensions: 274644
Number of successful extensions: 975
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 946
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 974
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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