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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_A08
         (574 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25240.1 68415.m03020 serpin, putative / serine protease inhi...    78   4e-15
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi...    75   4e-14
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi...    70   1e-12
At2g35580.1 68415.m04357 serpin family protein / serine protease...    67   9e-12
At1g64030.1 68414.m07252 serpin family protein / serine protease...    66   2e-11
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi...    64   5e-11
At1g62170.1 68414.m07013 serpin family protein / serine protease...    54   5e-08
At2g14540.1 68415.m01628 serpin family protein / serine protease...    48   4e-06
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi...    42   3e-04
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi...    40   0.002
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi...    36   0.019
At5g19600.1 68418.m02333 sulfate transporter, putative similar t...    31   0.72 
At4g00510.1 68417.m00070 cyclic nucleotide-binding domain-contai...    29   2.2  
At3g11910.1 68416.m01460 ubiquitin-specific protease, putative s...    29   2.2  
At5g50780.1 68418.m06291 ATP-binding region, ATPase-like domain-...    28   5.1  
At2g19710.1 68415.m02303 expressed protein   contains Pfam profi...    27   8.9  

>At2g25240.1 68415.m03020 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 324

 Score = 78.2 bits (184), Expect = 4e-15
 Identities = 53/180 (29%), Positives = 89/180 (49%), Gaps = 6/180 (3%)
 Frame = +3

Query: 3   DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNA 173
           DF S   +  D +N WA   T G IK  +S D+ID   ++   L N ++F+G W   F+A
Sbjct: 61  DFASKPSEVIDEVNTWAEVHTNGLIKQILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDA 120

Query: 174 SETKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDK 347
           + TK+ DFH+   T VK P M+  +  +         +++ LPY E    F M + LP+ 
Sbjct: 121 NMTKKNDFHLLDGTSVKVPFMTNYEDQYLRSYDGF--KVLRLPYIEDQRQFSMYIYLPND 178

Query: 348 LDGLSSVLDKAG-EKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIFN 524
            +GL+ +L+K G E    ++   L   +      +PKF+   + +   ++   G++  FN
Sbjct: 179 KEGLAPLLEKIGSEPSFFDNHIPLHCISVGA-FRIPKFKFSFEFNASEVLKDMGLTSPFN 237


>At3g45220.1 68416.m04880 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 393

 Score = 74.5 bits (175), Expect = 4e-14
 Identities = 53/174 (30%), Positives = 86/174 (49%), Gaps = 7/174 (4%)
 Frame = +3

Query: 21  KAADIINQ---WANEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETK 185
           K A++IN+   WA   T G IK  +SDD+I     +   L N ++F+G W   F+A  TK
Sbjct: 130 KPAEVINEVNAWAEVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTK 189

Query: 186 EKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDKLDGL 359
             DFH+   T+VK P M+  +   Y E  +   +++ LPY E    F M + LP+  DGL
Sbjct: 190 SYDFHLLDGTMVKVPFMTNYKKQ-YLEYYD-GFKVLRLPYVEDQRQFAMYIYLPNDRDGL 247

Query: 360 SSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
            ++L++   K    D    R        ++PKF+   +     ++ + G++  F
Sbjct: 248 PTLLEEISSKPRFLDNHIPRQRILTEAFKIPKFKFSFEFKASDVLKEMGLTLPF 301


>At2g26390.1 68415.m03167 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 389

 Score = 69.7 bits (163), Expect = 1e-12
 Identities = 51/172 (29%), Positives = 79/172 (45%), Gaps = 8/172 (4%)
 Frame = +3

Query: 30  DIINQWANEKTRGHIKSPISDDTIDP-----NAAAALFNVIFFQGHWHVPFNASETKEKD 194
           D +N WA+  T G IK  +S D  D      N+   L N ++F+  W   F+A  TK+ D
Sbjct: 133 DEVNIWADVHTNGLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDND 192

Query: 195 FHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDKLDGLSSV 368
           FH+     VK P M   +  +         Q++ LPY E    F M + LP+  DGL+++
Sbjct: 193 FHLLDGNTVKVPFMMSYKDQYLRGYDGF--QVLRLPYVEDKRHFSMYIYLPNDKDGLAAL 250

Query: 369 LDK-AGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
           L+K + E G L+    L  T     L +PK     +     ++   G++  F
Sbjct: 251 LEKISTEPGFLDSHIPLHRTPVDA-LRIPKLNFSFEFKASEVLKDMGLTSPF 301


>At2g35580.1 68415.m04357 serpin family protein / serine protease
           inhibitor family protein similar to protein zx [Hordeum
           vulgare subsp. vulgare] GI:19071, serpin [Triticum
           aestivum] GI:1885350; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 374

 Score = 66.9 bits (156), Expect = 9e-12
 Identities = 47/164 (28%), Positives = 76/164 (46%), Gaps = 6/164 (3%)
 Frame = +3

Query: 36  INQWANEKTRGHIKSPISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETKEKDFHVDST 212
           +N W  ++T G I + +  +         +F N +FF G W   F+ S TK+ DFH+   
Sbjct: 138 VNSWVEKQTNGLITNLLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDG 197

Query: 213 TIVKKPTM---SLLQPLFYTENKELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDK-A 380
           T V+ P M   S      Y   K +  Q          F M + LPD+ DGL S+L++ A
Sbjct: 198 TKVRVPFMTGASCRYTHVYEGFKVINLQYRRGREDSRSFSMQIYLPDEKDGLPSMLERLA 257

Query: 381 GEKGLLEDVFRLRPTNRKVK-LEMPKFEIKSKLDLKPLMPKFGV 509
             +G L+D   L   +  +K L++P+F+     +    +  FG+
Sbjct: 258 STRGFLKDNEVLPSHSAVIKELKIPRFKFDFAFEASEALKGFGL 301


>At1g64030.1 68414.m07252 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311, serpin [Triticum
           aestivum] GI:871551; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 385

 Score = 65.7 bits (153), Expect = 2e-11
 Identities = 49/179 (27%), Positives = 80/179 (44%), Gaps = 10/179 (5%)
 Frame = +3

Query: 3   DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASE 179
           DF S+ ++    +N W    T   IK  + D ++         N + F+G W  PF    
Sbjct: 127 DFRSEAEEVRKEVNSWVEHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYY 186

Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKESG------FRMLVLLP 341
           T++ DF++ + T V  P MS  +  +         +++ LPY+         F M   LP
Sbjct: 187 TRDNDFYLVNGTSVSVPFMSSYENQYVRAYDGF--KVLRLPYQRGSDDTNRKFSMYFYLP 244

Query: 342 DKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRK--VKLEMPKFEIKSKLDLKPLMPKFGV 509
           DK DGL  +L+K A   G L+      PT R    K  +PKF+I+    +  ++ + G+
Sbjct: 245 DKKDGLDDLLEKMASTPGFLDSHI---PTYRDELEKFRIPKFKIEFGFSVTSVLDRLGL 300


>At1g47710.1 68414.m05302 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 391

 Score = 64.5 bits (150), Expect = 5e-11
 Identities = 43/168 (25%), Positives = 79/168 (47%), Gaps = 5/168 (2%)
 Frame = +3

Query: 36  INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
           +N WA ++T G I   + + + D        N ++F+G W+  F+ S T+E +FH+    
Sbjct: 138 VNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGN 197

Query: 216 IVKKPTMSLLQPLFYTENKELEAQLVELPYKES----GFRMLVLLPDKLDGLSSVLDK-A 380
            V  P M+  +  + +       +++ LPY +      F M   LPD  +GLS +LDK  
Sbjct: 198 KVTAPFMTSKKKQYVSAYDGF--KVLGLPYLQGQDKRQFSMYFYLPDANNGLSDLLDKIV 255

Query: 381 GEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIFN 524
              G L++    R    + + ++PKF+     D   ++   G++  F+
Sbjct: 256 STPGFLDNHIPRRQVKVR-EFKIPKFKFSFGFDASNVLKGLGLTSPFS 302


>At1g62170.1 68414.m07013 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           GI:9937311 from [Cucurbita maxima]; contains Pfam
           profile PF00079: Serpin (serine protease inhibitor)
          Length = 433

 Score = 54.4 bits (125), Expect = 5e-08
 Identities = 45/165 (27%), Positives = 76/165 (46%), Gaps = 10/165 (6%)
 Frame = +3

Query: 3   DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASE 179
           DF S  ++    +N WA+  T G IK  +   ++         + ++F+G W   ++ S 
Sbjct: 191 DFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKSM 250

Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES------GFRMLVLLP 341
           TK K F++ + T V  P MS  +  +         +++ LPY++        F M + LP
Sbjct: 251 TKCKPFYLLNGTSVSVPFMSSFEKQYIAAYDGF--KVLRLPYRQGRDNTNRNFAMYIYLP 308

Query: 342 DKLDGLSSVLDK-AGEKGLLEDVFRLRPTNR-KV-KLEMPKFEIK 467
           DK   L  +L++     G L+      P  R KV K  +PKF+I+
Sbjct: 309 DKKGELDDLLERMTSTPGFLDS---HNPERRVKVGKFRIPKFKIE 350


>At2g14540.1 68415.m01628 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 407

 Score = 48.0 bits (109), Expect = 4e-06
 Identities = 31/120 (25%), Positives = 55/120 (45%), Gaps = 6/120 (5%)
 Frame = +3

Query: 36  INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
           +N WA+  T   IK  +   ++         N ++F+G W   F+ S T++K FH+ +  
Sbjct: 167 VNTWASRHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAWEKAFDKSMTRDKPFHLLNGK 226

Query: 216 IVKKPTMSLLQPLFYTENKELEAQLVELPYKES------GFRMLVLLPDKLDGLSSVLDK 377
            V  P M   +  F         +++ LPY++        F M + LPDK   L ++L++
Sbjct: 227 SVSVPFMRSYEKQFIEAYDGF--KVLRLPYRQGRDDTNREFSMYLYLPDKKGELDNLLER 284


>At1g63280.1 68414.m07154 serpin-related / serine protease
           inhibitor-related similar to protein zx [Hordeum vulgare
           subsp. vulgare] GI:19071, serpin [Triticum aestivum]
           GI:1885346
          Length = 120

 Score = 41.9 bits (94), Expect = 3e-04
 Identities = 16/55 (29%), Positives = 29/55 (52%)
 Frame = +3

Query: 36  INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFH 200
           +N+WA++ T G I   +   ++         N ++F+G W   F+ S TK+ +FH
Sbjct: 16  LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFH 70


>At1g51330.1 68414.m05772 serpin-related / serine protease
           inhibitor-related similar to serpin [Hordeum vulgare
           subsp. vulgare] CAA64599.1  GI:1197577
          Length = 193

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 3/96 (3%)
 Frame = +3

Query: 36  INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
           +N WA   T G IK+ +   ++         N ++F+G W   F  S T  K FH+ +  
Sbjct: 39  VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98

Query: 216 IVKKPTMSLLQPLF---YTENKELEAQLVELPYKES 314
            V  P M   +  +   Y   K L      + YK++
Sbjct: 99  QVLVPFMKSYERKYMKAYNGFKVLRILQYRVDYKDT 134


>At1g64010.1 68414.m07250 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 185

 Score = 35.9 bits (79), Expect = 0.019
 Identities = 32/120 (26%), Positives = 60/120 (50%), Gaps = 7/120 (5%)
 Frame = +3

Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKESG-----FRMLVLLPD 344
           TK++DFH+ + T V    MS  +   Y E  +   ++++LP+++       F M   LPD
Sbjct: 2   TKDRDFHLINGTSVSVSLMSSYKDQ-YIEAYD-GFKVLKLPFRQGNDTSRNFSMHFYLPD 59

Query: 345 KLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKV-KLEMPKFEIKSKLDLKPLMPKFGVSKI 518
           + DGL ++++K A   G L+    +     KV +  +PKF+I+          + G+ ++
Sbjct: 60  EKDGLDNLVEKMASSVGFLDS--HIPSQKVKVGEFGIPKFKIEFGFSASRAFNRLGLDEM 117


>At5g19600.1 68418.m02333 sulfate transporter, putative similar to
           sulfate transporter [Arabidopsis thaliana] GI:2285885;
           contains Pfam profiles PF00916: Sulfate transporter
           family, PF01740: STAS domain; supporting cDNA
           gi|14141683|dbj|AB061739.1|
          Length = 634

 Score = 30.7 bits (66), Expect = 0.72
 Identities = 25/96 (26%), Positives = 45/96 (46%)
 Frame = +3

Query: 234 MSLLQPLFYTENKELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLLEDVFR 413
           + L  P+F+  +  +  +++     E      +LL   L G+S++ D  G + LLE    
Sbjct: 521 LQLGSPVFFANSTYVRERILRWIRDEPEAIEFLLLD--LSGVSTI-DMTGMETLLEIQRI 577

Query: 414 LRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
           L   N K+ +  P+FE+  K+ L   + K G   +F
Sbjct: 578 LGSKNIKMVIINPRFEVLEKMMLSHFVEKIGKEYMF 613


>At4g00510.1 68417.m00070 cyclic nucleotide-binding
           domain-containing protein contains Pfam profile: PF00027
           cyclic nucleotide-binding domain
          Length = 175

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 17/49 (34%), Positives = 24/49 (48%)
 Frame = +3

Query: 357 LSSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKF 503
           + SV D   E+ L+ED+  L P + +V    P F   S L +K L   F
Sbjct: 118 IKSVSDSDKERCLVEDILYLEPLDVRVSPNCPSFSSISVLMIKVLTIHF 166


>At3g11910.1 68416.m01460 ubiquitin-specific protease, putative
           strong similarity to ubiquitin-specific protease 12
           (UBP12) [Arabidopsis thaliana] GI:11993471; contains
           Pfam profiles PF00443: Ubiquitin carboxyl-terminal
           hydrolase, PF00917: MATH domain
          Length = 1115

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 23/69 (33%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
 Frame = +3

Query: 252 LFYTENKEL-EAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLLEDVFRLRPTN 428
           L Y +N+EL   + +E P KE  F M +    KL     V+++  EK  L+D  +LR T+
Sbjct: 794 LEYVQNRELVRFRTLEKP-KEDEFTMEL---SKLHTYDDVVERVAEKLGLDDPSKLRLTS 849

Query: 429 RKVKLEMPK 455
                + PK
Sbjct: 850 HNCYSQQPK 858


>At5g50780.1 68418.m06291 ATP-binding region, ATPase-like
           domain-containing protein low similarity to microrchidia
           [Homo sapiens] GI:5410257; contains Pfam profile
           PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
           HSP90-like domain protein
          Length = 823

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
 Frame = +3

Query: 273 ELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLL--EDVFRLRPTNRKVKLE 446
           EL  +  ELP  +SG R L  L  + + L   LDK  E  LL  +D+ R R   + ++ E
Sbjct: 702 ELPGKSSELPKPQSGPRTLSQLEQENNELRERLDKKEEVFLLLQKDLRRERELRKTLEAE 761

Query: 447 MPKFEIKSK 473
           +   + K K
Sbjct: 762 VETLKNKLK 770


>At2g19710.1 68415.m02303 expressed protein   contains Pfam profile:
           PF03398 eukaryotic protein of unknown function, DUF292
          Length = 937

 Score = 27.1 bits (57), Expect = 8.9
 Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
 Frame = -1

Query: 463 ISNFGISNFTFLLVGLSLNTSSRRPFSPA-LSNTDDKPSSLSGSRTNIRKPDSLYGNS 293
           I +FG S+   L    + NT +R  F P   S++D+  S +        K DSLY +S
Sbjct: 722 IDDFGQSSRKDLYSKKASNTETRPSFMPPHPSSSDEDDSDMQHPGRTETKSDSLYSHS 779


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,882,374
Number of Sequences: 28952
Number of extensions: 275185
Number of successful extensions: 968
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 926
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 959
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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