Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_B10_e74_04.seq
(1430 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66560.1 68418.m08391 phototropic-responsive NPH3 family prot... 31 1.4
At3g26280.1 68416.m03279 cytochrome P450 family protein identica... 31 1.4
At4g32470.1 68417.m04622 ubiquinol-cytochrome C reductase comple... 31 1.9
At5g25450.1 68418.m03023 ubiquinol-cytochrome C reductase comple... 30 3.2
At3g61570.1 68416.m06896 intracellular protein transport protein... 30 4.3
At2g36580.1 68415.m04486 pyruvate kinase, putative similar to py... 29 5.7
At1g33560.1 68414.m04153 disease resistance protein (CC-NBS-LRR ... 29 5.7
At1g03780.2 68414.m00358 targeting protein-related similar to mi... 26 6.2
At1g03780.1 68414.m00359 targeting protein-related similar to mi... 26 6.2
At4g32470.2 68417.m04623 ubiquinol-cytochrome C reductase comple... 29 7.5
At3g52990.1 68416.m05841 pyruvate kinase, putative similar to py... 29 7.5
At2g25660.1 68415.m03075 expressed protein 29 7.5
At1g75530.1 68414.m08778 forkhead-associated domain-containing p... 29 9.9
>At5g66560.1 68418.m08391 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 668
Score = 31.5 bits (68), Expect = 1.4
Identities = 20/57 (35%), Positives = 31/57 (54%)
Frame = +1
Query: 442 LKEFRNDNRKVSVSDQTREYMDAVIDLVDAYLKAVCKIHSELKALRVKRALDIRKLS 612
LK + N +S+ DQ R Y D + VD YLKA + SE + ++ +D +KL+
Sbjct: 447 LKSDKFYNLAISLPDQARLYDDGLYRAVDVYLKAHPWV-SEAEREKICGVMDCQKLT 502
>At3g26280.1 68416.m03279 cytochrome P450 family protein identical
to cytochrome P450 monooxygenase (CYP71B4) GB:D78603
[Arabidopsis thaliana] (Plant Mol. Biol. 37 (1), 39-52
(1998))
Length = 504
Score = 31.5 bits (68), Expect = 1.4
Identities = 31/140 (22%), Positives = 64/140 (45%), Gaps = 12/140 (8%)
Frame = +1
Query: 316 LLEHRDRYLSF--FNNESENDMSELDRDQIDTGAQRIINTCSHL------LKEFRNDNRK 471
++++ +Y SF + + +S + IDT A +I + L +K+ +++ R
Sbjct: 278 MIDNEQQYASFKLTVDHLKGVLSNIYHAGIDTSAITLIWAMAELVRNPRVMKKAQDEIRT 337
Query: 472 VSVSDQTREYMDAVIDLVDAYLKAVCK----IHSELKALRVKRALDIRKLSRLELPQSKS 639
Q M+ +D + YLK V K +H L + + K+ ++PQ ++
Sbjct: 338 CIGIKQEGRIMEEDLDKLQ-YLKLVVKETLRLHPAAPLLLPRETMADIKIQGYDIPQKRA 396
Query: 640 IILNPFVSDKDPEKRKTLQE 699
+++N + +DPE K +E
Sbjct: 397 LLVNAWSIGRDPESWKNPEE 416
>At4g32470.1 68417.m04622 ubiquinol-cytochrome C reductase complex
14 kDa protein, putative similar to SP|P48502
Ubiquinol-cytochrome C reductase complex 14 kDa protein
(EC 1.10.2.2) (CR14) {Solanum tuberosum}; contains Pfam
profile PF02271: Ubiquinol-cytochrome C reductase
complex 14kD subunit
Length = 122
Score = 31.1 bits (67), Expect = 1.9
Identities = 19/77 (24%), Positives = 36/77 (46%)
Frame = +1
Query: 298 TRLRDFLLEHRDRYLSFFNNESENDMSELDRDQIDTGAQRIINTCSHLLKEFRNDNRKVS 477
TRLR + L + D Y +++ + + M+ L R+ +D QR+ +K +
Sbjct: 26 TRLRRYGLRYDDLYDQYYSMDIKEAMNRLPREVVDARNQRLKRAMDLSMKHEYLPKDLQA 85
Query: 478 VSDQTREYMDAVIDLVD 528
V R Y+ ++ LV+
Sbjct: 86 VQTPFRGYLQDMLALVE 102
>At5g25450.1 68418.m03023 ubiquinol-cytochrome C reductase complex
14 kDa protein, putative similar to SP|P48502
Ubiquinol-cytochrome C reductase complex 14 kDa protein
(EC 1.10.2.2) (CR14) {Solanum tuberosum}; contains Pfam
profile PF02271: Ubiquinol-cytochrome C reductase
complex 14kD subunit
Length = 122
Score = 30.3 bits (65), Expect = 3.2
Identities = 20/76 (26%), Positives = 39/76 (51%), Gaps = 1/76 (1%)
Frame = +1
Query: 301 RLRDFLLEHRDRYLSFFNNESENDMSELDRDQIDTGAQRIINTCSHLLK-EFRNDNRKVS 477
RLR + L + D Y ++ + + ++ L R+ +D QR++ +K E+ DN + +
Sbjct: 27 RLRRYGLRYDDLYDPLYDLDIKEALNRLPREIVDARNQRLMRAMDLSMKHEYLPDNLQ-A 85
Query: 478 VSDQTREYMDAVIDLV 525
V R Y+ ++ LV
Sbjct: 86 VQTPFRSYLQDMLALV 101
>At3g61570.1 68416.m06896 intracellular protein transport protein
USO1-related contains weak similarity to intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]
Length = 712
Score = 29.9 bits (64), Expect = 4.3
Identities = 40/162 (24%), Positives = 79/162 (48%), Gaps = 9/162 (5%)
Frame = +1
Query: 220 EDKQRLLRSKTKNGFITTAKDICSQITRLRDFLL---EHRDRYLSFFNNESENDMSELDR 390
+D +L +TK+ A ++ + +LRDF L E R R SF + E + LD+
Sbjct: 180 KDLADMLEDRTKSMAAVQATELAKEREKLRDFQLSLQEERKRSESF---KEELESMRLDK 236
Query: 391 DQIDTGAQRIINTCSHLLKEFRNDNRKVSVSDQTREYMDAVIDLVDAYLKAVCKIHSELK 570
++ ++ + L E + + ++ ++ Q + ++ + KA+ K ++ELK
Sbjct: 237 NKTSMEISKMRSELDAKLLEIK--HLQMKLTGQESHAIGPGMEHLKEVNKALEKENNELK 294
Query: 571 ALR--VKRAL-DIRKL--SRLELPQSKSIILNPFVSDKD-PE 678
R ++ AL + RKL S++ ++S+ +P DK+ PE
Sbjct: 295 LKRSELEAALEESRKLTNSKVFPDATESLTRHPSTLDKEKPE 336
>At2g36580.1 68415.m04486 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 527
Score = 29.5 bits (63), Expect = 5.7
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 377 PSWTAIK*TLGPKESSIHVLTY*KSSGMIIAKF 475
P+ T I TLGPK S+ V+ +GM +A+F
Sbjct: 27 PALTKIVGTLGPKSRSVEVIAGCLKAGMSVARF 59
>At1g33560.1 68414.m04153 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 787
Score = 29.5 bits (63), Expect = 5.7
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 3/54 (5%)
Frame = +1
Query: 226 KQRLLRSKTKNGFITTAKDICSQITRLRDFLLEHRDRYL---SFFNNESENDMS 378
K L+ K KN F+ T+ DI L D ++H D L S F S N +S
Sbjct: 604 KIHLIFCKVKNSFVQTSFDISKIFPSLSDLTIDHCDDLLELKSIFGITSLNSLS 657
>At1g03780.2 68414.m00358 targeting protein-related similar to
microtubule-associated protein / targeting protein for
Xklp2 ((TPX2) GI:8926138) {Homo sapiens}; similar to
Restricted expression proliferation associated protein
100 (p100) (Differentially expressed in lung cells 2)
(DIL-2) (Targeting protein for Xklp2) (C20orf1 protein)
(C20orf2 protein) (Protein FLS353)(SP:Q9ULW0) {Homo
sapiens}
Length = 725
Score = 25.8 bits (54), Expect(2) = 6.2
Identities = 10/35 (28%), Positives = 17/35 (48%)
Frame = +1
Query: 199 GIQSPVSEDKQRLLRSKTKNGFITTAKDICSQITR 303
G + K L KT+NG + T ++C +T+
Sbjct: 238 GKSRQILNPKPATLLHKTRNGLVNTGFNLCPSVTK 272
Score = 21.8 bits (44), Expect(2) = 6.2
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +1
Query: 424 NTCSHLLKEFRNDNRKVSVSDQTREYM 504
N C + K +NRKV V +Q ++
Sbjct: 265 NLCPSVTKHTPKENRKVYVREQIAPFV 291
>At1g03780.1 68414.m00359 targeting protein-related similar to
microtubule-associated protein / targeting protein for
Xklp2 ((TPX2) GI:8926138) {Homo sapiens}; similar to
Restricted expression proliferation associated protein
100 (p100) (Differentially expressed in lung cells 2)
(DIL-2) (Targeting protein for Xklp2) (C20orf1 protein)
(C20orf2 protein) (Protein FLS353)(SP:Q9ULW0) {Homo
sapiens}
Length = 687
Score = 25.8 bits (54), Expect(2) = 6.2
Identities = 10/35 (28%), Positives = 17/35 (48%)
Frame = +1
Query: 199 GIQSPVSEDKQRLLRSKTKNGFITTAKDICSQITR 303
G + K L KT+NG + T ++C +T+
Sbjct: 238 GKSRQILNPKPATLLHKTRNGLVNTGFNLCPSVTK 272
Score = 21.8 bits (44), Expect(2) = 6.2
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +1
Query: 424 NTCSHLLKEFRNDNRKVSVSDQTREYM 504
N C + K +NRKV V +Q ++
Sbjct: 265 NLCPSVTKHTPKENRKVYVREQIAPFV 291
>At4g32470.2 68417.m04623 ubiquinol-cytochrome C reductase complex
14 kDa protein, putative similar to SP|P48502
Ubiquinol-cytochrome C reductase complex 14 kDa protein
(EC 1.10.2.2) (CR14) {Solanum tuberosum}; contains Pfam
profile PF02271: Ubiquinol-cytochrome C reductase
complex 14kD subunit
Length = 101
Score = 29.1 bits (62), Expect = 7.5
Identities = 13/41 (31%), Positives = 24/41 (58%)
Frame = +1
Query: 298 TRLRDFLLEHRDRYLSFFNNESENDMSELDRDQIDTGAQRI 420
TRLR + L + D Y +++ + + M+ L R+ +D QR+
Sbjct: 26 TRLRRYGLRYDDLYDQYYSMDIKEAMNRLPREVVDARNQRL 66
>At3g52990.1 68416.m05841 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 527
Score = 29.1 bits (62), Expect = 7.5
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 377 PSWTAIK*TLGPKESSIHVLTY*KSSGMIIAKF 475
P+ T I TLGPK S+ L+ +GM +A+F
Sbjct: 27 PALTKIVGTLGPKSRSVEALSGCLKAGMSVARF 59
>At2g25660.1 68415.m03075 expressed protein
Length = 2146
Score = 29.1 bits (62), Expect = 7.5
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = +1
Query: 214 VSEDKQRLLRSKTKNGFITTAKDICSQITRLRDFLLEHRDRY 339
+S + S++K+ FI + +++C Q LRD L E R Y
Sbjct: 1336 LSRSTDPAVHSRSKDLFIQSVQNLCLQAENLRDLLEEIRGYY 1377
>At1g75530.1 68414.m08778 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 555
Score = 28.7 bits (61), Expect = 9.9
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -2
Query: 415 FGPQCLFDRGPTRTCHFQTHC*RNSNTDPGVQVRNLSVSLFVN 287
F PQ LFD P RT ++ T R + ++V N ++ +N
Sbjct: 72 FVPQSLFDSVPVRTHYYTTRKRRRLELEESLKVNNNAIDEHLN 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,083,698
Number of Sequences: 28952
Number of extensions: 343042
Number of successful extensions: 757
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 757
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3778328064
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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