Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030623sawa_A12_e89_02.seq
(1445 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 34 0.27
At2g41860.2 68415.m05174 calcium-dependent protein kinase, putat... 32 1.1
At2g41860.1 68415.m05173 calcium-dependent protein kinase, putat... 32 1.1
At4g32060.1 68417.m04563 calcium-binding EF hand family protein ... 31 1.9
At3g22760.1 68416.m02870 CXC domain containing TSO1-like protein... 31 2.5
At4g36070.1 68417.m05135 calcium-dependent protein kinase family... 25 3.0
At5g55630.2 68418.m06937 outward rectifying potassium channel (K... 30 3.3
At5g55630.1 68418.m06936 outward rectifying potassium channel (K... 30 3.3
At1g70180.2 68414.m08076 sterile alpha motif (SAM) domain-contai... 29 5.7
At1g70180.1 68414.m08075 sterile alpha motif (SAM) domain-contai... 29 5.7
At2g03000.1 68415.m00252 zinc finger (C3HC4-type RING finger) fa... 29 7.6
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 33.9 bits (74), Expect = 0.27
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 404 PNEPPRPSGDATANTTAAPKPIAGNENLIQNPIRTPTPNARDL 532
P +P P AT+ AP P + I P++ PTP++ D+
Sbjct: 819 PPKPVTPLPPATSPMANAPTPSSSESGEISTPVQAPTPDSEDI 861
>At2g41860.2 68415.m05174 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 530
Score = 31.9 bits (69), Expect = 1.1
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +1
Query: 583 DVDKDGLISFNDFVLFAERFKSLGHLDEKQAKEFS 687
DVDKDG + N+FV + + LG+ DE K F+
Sbjct: 404 DVDKDGYLDVNEFVAISVHIRKLGN-DEHLKKAFT 437
>At2g41860.1 68415.m05173 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 7 [Arabidopsis thaliana]
gi|1399277|gb|AAB03247
Length = 425
Score = 31.9 bits (69), Expect = 1.1
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +1
Query: 583 DVDKDGLISFNDFVLFAERFKSLGHLDEKQAKEFS 687
DVDKDG + N+FV + + LG+ DE K F+
Sbjct: 299 DVDKDGYLDVNEFVAISVHIRKLGN-DEHLKKAFT 332
>At4g32060.1 68417.m04563 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 498
Score = 31.1 bits (67), Expect = 1.9
Identities = 22/64 (34%), Positives = 30/64 (46%)
Frame = +1
Query: 577 ILDVDKDGLISFNDFVLFAERFKSLGHLDEKQAKEFSDIIKMTWEEQWGAIDPYNFVTVE 756
+ DVD DGLISF +++ F +L + E F+ KM + G ID F TV
Sbjct: 220 LFDVDNDGLISFKEYIFFV----TLLSIPE---SSFAVAFKMFDTDNNGEIDKEEFKTVM 272
Query: 757 QYFR 768
R
Sbjct: 273 SLMR 276
>At3g22760.1 68416.m02870 CXC domain containing TSO1-like protein 1
(SOL1) identical to CXC domain containing TSO1-like
protein 1 (SOL1) [Arabidopsis thaliana] GI:7767427;
contains Pfam profile PF03638: Tesmin/TSO1-like CXC
domain; supporting cDNA
gi|7767426|gb|AF205142.1|AF205142
Length = 609
Score = 30.7 bits (66), Expect = 2.5
Identities = 18/51 (35%), Positives = 29/51 (56%)
Frame = +2
Query: 476 NENLIQNPIRTPTPNARDLIVQNSGVVRCEPSTTSWM*TRTG*SRSMISSF 628
+ENLI +PI T +PN++ + + + P +T W R G R++I SF
Sbjct: 555 SENLIHSPITTLSPNSKRVSLSHLD----SPESTPW--RRNGGGRNLIRSF 599
>At4g36070.1 68417.m05135 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 536
Score = 25.0 bits (52), Expect(2) = 3.0
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +1
Query: 538 SEFWRRKMRTVHNILDVDKDGLIS 609
SE W+++ R + D+D DG I+
Sbjct: 448 SEKWQQRSRAAFDKFDIDGDGFIT 471
Score = 23.8 bits (49), Expect(2) = 3.0
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1
Query: 583 DVDKDGLISFNDF 621
DVD+DG IS N+F
Sbjct: 498 DVDEDGRISINEF 510
>At5g55630.2 68418.m06937 outward rectifying potassium channel
(KCO1) identical to kco1 [Arabidopsis thaliana]
gi|2230761|emb|CAA69158 of the 2 pore, 4 transmembrane
(2P/4TM) K+ channel family, PMID:11500563
Length = 363
Score = 30.3 bits (65), Expect = 3.3
Identities = 17/73 (23%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = +1
Query: 547 WRRKMRTVHNIL---DVDKDGLISFNDFVLFAERFKSLGHLDEKQAKEFSDIIKMTWEEQ 717
W R +N L D+D+DG++ +F+++ + K +G +DEK D + ++
Sbjct: 286 WVLTRRITNNDLEAADLDEDGVVGAAEFIVY--KLKEMGKIDEKDISGIMDEFEQLDYDE 343
Query: 718 WGAIDPYNFVTVE 756
G + + V +
Sbjct: 344 SGTLTTSDIVLAQ 356
>At5g55630.1 68418.m06936 outward rectifying potassium channel
(KCO1) identical to kco1 [Arabidopsis thaliana]
gi|2230761|emb|CAA69158 of the 2 pore, 4 transmembrane
(2P/4TM) K+ channel family, PMID:11500563
Length = 363
Score = 30.3 bits (65), Expect = 3.3
Identities = 17/73 (23%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = +1
Query: 547 WRRKMRTVHNIL---DVDKDGLISFNDFVLFAERFKSLGHLDEKQAKEFSDIIKMTWEEQ 717
W R +N L D+D+DG++ +F+++ + K +G +DEK D + ++
Sbjct: 286 WVLTRRITNNDLEAADLDEDGVVGAAEFIVY--KLKEMGKIDEKDISGIMDEFEQLDYDE 343
Query: 718 WGAIDPYNFVTVE 756
G + + V +
Sbjct: 344 SGTLTTSDIVLAQ 356
>At1g70180.2 68414.m08076 sterile alpha motif (SAM)
domain-containing protein contains Pfam profile PF00536:
SAM domain (Sterile alpha motif)
Length = 460
Score = 29.5 bits (63), Expect = 5.7
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = -1
Query: 653 PRDLNLSAKRTKSLNEISPSLSTSRMLWTVRILRRQNSER 534
PRD LS+ RT + S S+S W + LRR++ ER
Sbjct: 130 PRDRPLSSSRTARGSSQMISSSSSHPAWALEDLRRRSPER 169
>At1g70180.1 68414.m08075 sterile alpha motif (SAM)
domain-containing protein contains Pfam profile PF00536:
SAM domain (Sterile alpha motif)
Length = 456
Score = 29.5 bits (63), Expect = 5.7
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = -1
Query: 653 PRDLNLSAKRTKSLNEISPSLSTSRMLWTVRILRRQNSER 534
PRD LS+ RT + S S+S W + LRR++ ER
Sbjct: 130 PRDRPLSSSRTARGSSQMISSSSSHPAWALEDLRRRSPER 169
>At2g03000.1 68415.m00252 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 535
Score = 29.1 bits (62), Expect = 7.6
Identities = 32/135 (23%), Positives = 61/135 (45%), Gaps = 4/135 (2%)
Frame = +2
Query: 365 SVRGGRRANLASWPNEPPRPSGDATANTTAAPKPI-AGNENLIQNPIRTPTPNARDLIVQ 541
S R +A++++ P S + +T+ P+ + + N+I + + +Q
Sbjct: 117 STRRSVQASMSARETVPSSTSTRSMQTSTSTPEIMPTSSRNVITSSEEVANTFTQTSYIQ 176
Query: 542 NSGV--VRCEPSTTSWM*TRTG*S-RSMISSFSLRGSNPSGISTRSRRRNSVILLR*RGR 712
+ PST SW + S R + +S S RG++P STR ++S+ GR
Sbjct: 177 FGSLWETNTTPSTRSWPTVPSSNSPRVLQTSMSRRGTSPMSSSTRRNVQSSM-----SGR 231
Query: 713 NSGALSIPTTS*QSN 757
+ + S T+S Q++
Sbjct: 232 ETVSSSTSTSSMQTS 246
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,585,743
Number of Sequences: 28952
Number of extensions: 456981
Number of successful extensions: 2197
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1426
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2164
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3826521024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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