Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030623sawa_A11_e81_01.seq
         (1565 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g30630.1 68414.m03746 coatomer protein epsilon subunit family...    94   2e-19
At2g34840.1 68415.m04278 coatomer protein epsilon subunit family...    87   3e-17
At5g01270.1 68418.m00036 double-stranded RNA-binding domain (DsR...    31   1.6  
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t...    31   2.1  

>At1g30630.1 68414.m03746 coatomer protein epsilon subunit family
           protein / COPE family protein similar to SP|O14579
           Coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP) from Homo sapiens, SP|Q60445 from
           Cricetulus griseus; ESTs gb|Z17908, gb|AA728673,
           gb|N96555, gb|H76335, gb|AA712463, gb|W43247, gb|T45611,
           gb|T21160, gb|T14119 and AI100483 come from this gene
          Length = 292

 Score = 94.3 bits (224), Expect = 2e-19
 Identities = 50/153 (32%), Positives = 85/153 (55%), Gaps = 1/153 (0%)
 Frame = +1

Query: 61  RXFGTRGNHRIVLQEL-KTADPILQPLKSLVDYLSPGANKPAIVADIDARVAKGTELSNE 237
           R +   G++++V+ E+ + A   LQ +K L  YLS   NK + ++ +   +A  T  +N 
Sbjct: 48  RAYIALGSYQLVISEIDEAAATPLQAVKLLAMYLSSPENKESTISSLREWLADPTVGNNA 107

Query: 238 VFHVVAATIYYYEDNYEAALKIFHDIELVELRAYSIQCLLAMNRPDLARXXXXXXXXXXD 417
           +  ++A TI+ +E++Y  ALK  H    ++L A ++Q  + M+R D A           +
Sbjct: 108 IIRLIAGTIFMHEEDYNEALKHTHSGGTMDLHALNVQIFIKMHRSDFAEKQLRVMQQIDE 167

Query: 418 DGTLTQLAQAWINLSQGGPGVQDAHYSIMELSE 516
           D TLTQLA AW+NL+ GG  +Q+A+    + SE
Sbjct: 168 DHTLTQLASAWLNLAVGGSKIQEAYLIFQDFSE 200


>At2g34840.1 68415.m04278 coatomer protein epsilon subunit family
           protein / COPE family protein similar to SP|O14579
           Coatomer epsilon subunit (Epsilon-coat protein)
           (Epsilon-COP) from Homo sapiens, SP|Q60445 from
           Cricetulus griseus
          Length = 293

 Score = 87.0 bits (206), Expect = 3e-17
 Identities = 47/153 (30%), Positives = 84/153 (54%), Gaps = 1/153 (0%)
 Frame = +1

Query: 61  RXFGTRGNHRIVLQEL-KTADPILQPLKSLVDYLSPGANKPAIVADIDARVAKGTELSNE 237
           R +   G++++V+ E+ ++A   LQ +K L  YLS   NK + ++ +   +A  T  +N+
Sbjct: 49  RSYIALGSYQLVISEIDESAATPLQAVKLLAMYLSTPQNKESTISSLKEWLADSTIGNND 108

Query: 238 VFHVVAATIYYYEDNYEAALKIFHDIELVELRAYSIQCLLAMNRPDLARXXXXXXXXXXD 417
              ++A  I+ +E++Y   LK  H    ++L A ++Q  + M+R + A           +
Sbjct: 109 TLRLIAGIIFMHEEDYNETLKHTHAGGTMDLYALNVQIFIKMHRAEYAEKQLRVMQQIDE 168

Query: 418 DGTLTQLAQAWINLSQGGPGVQDAHYSIMELSE 516
           D TLTQLA AW+NL+ GG  +Q+A+    + SE
Sbjct: 169 DHTLTQLASAWLNLAVGGSKIQEAYLIFEDFSE 201


>At5g01270.1 68418.m00036 double-stranded RNA-binding domain
           (DsRBD)-containing protein contains Pfam profile
           PF00035: Double-stranded RNA binding motif
          Length = 771

 Score = 31.5 bits (68), Expect = 1.6
 Identities = 25/78 (32%), Positives = 37/78 (47%), Gaps = 3/78 (3%)
 Frame = +1

Query: 85  HRIVLQELKTADPILQP--LKSLVDYLSPGANKPAIVADIDARVAKGTELSNEVFHVVAA 258
           H I L+EL+     ++P   KSL+   + G   P +   ID R+    +      HVV+A
Sbjct: 302 HLISLKELRDRIVCVKPDAKKSLLSVFNGGICHPKMAMVIDDRMKVWEDKDQPRVHVVSA 361

Query: 259 TIYYYEDNYEA-ALKIFH 309
            + YY    EA AL + H
Sbjct: 362 YLPYYAPQAEACALVVPH 379


>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
           AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam profile: PF00501 AMP-binding enzyme;
           identical to cDNA adenosine monophosphate binding
           protein 8 AMPBP8 (AMPBP8)  GI:20799724
          Length = 544

 Score = 31.1 bits (67), Expect = 2.1
 Identities = 16/33 (48%), Positives = 22/33 (66%)
 Frame = +1

Query: 91  IVLQELKTADPILQPLKSLVDYLSPGANKPAIV 189
           IVLQ L  +    QPLKS V++L+ G++ PA V
Sbjct: 281 IVLQILSASQESDQPLKSPVNFLTAGSSPPATV 313


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,180,508
Number of Sequences: 28952
Number of extensions: 272354
Number of successful extensions: 614
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4212064704
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)