Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdV40090
(846 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 142 6e-35
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 136 5e-33
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 85 2e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 2e-09
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.029
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 30 0.36
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 27 3.3
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 142 bits (344), Expect = 6e-35
Identities = 74/163 (45%), Positives = 99/163 (60%), Gaps = 1/163 (0%)
Frame = +2
Query: 254 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 433
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186
Query: 434 EPYNSILTTHTTLEHSDCAFMVDNEPSMTSAAVISTLSAQPTPT*IVXXXXXXXXXXXXX 613
EPYNS+LTTH TL++SDC FMVDNE + +PT +
Sbjct: 187 EPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIE-RPTYENLNRLIAQVVSSITAS 245
Query: 614 XXTAL*MWTSPSS-RLNLVP*PRIHFPLVTYAPVHLCREGYHE 739
A + + + NLVP PRIHFPLVTY+P+ + +HE
Sbjct: 246 LRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHE 288
Score = 134 bits (323), Expect = 2e-32
Identities = 55/77 (71%), Positives = 69/77 (89%)
Frame = +3
Query: 24 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 203
+D F TFFSETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYAR
Sbjct: 50 NDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109
Query: 204 GHYTIGKEIVDLVLDRI 254
GHYT+GKE++D VL+RI
Sbjct: 110 GHYTVGKEMIDSVLERI 126
Score = 93.9 bits (223), Expect = 3e-20
Identities = 46/57 (80%), Positives = 48/57 (84%)
Frame = +1
Query: 514 YDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQT*LGALTPYP 684
YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQT L PYP
Sbjct: 214 YDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQT---NLVPYP 267
Score = 41.9 bits (94), Expect = 1e-04
Identities = 22/34 (64%), Positives = 23/34 (67%)
Frame = +3
Query: 714 ISAEKATMNSFSVAEIPNACFEARPNQMVKCDPR 815
+SA KA S SV EI N CFE NQMVKCDPR
Sbjct: 280 VSAAKAFHESNSVQEITNQCFEPY-NQMVKCDPR 312
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 136 bits (328), Expect = 5e-33
Identities = 58/77 (75%), Positives = 69/77 (89%)
Frame = +3
Query: 24 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 203
D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYAR
Sbjct: 46 DGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105
Query: 204 GHYTIGKEIVDLVLDRI 254
GHYT+GKE+VD V D+I
Sbjct: 106 GHYTVGKELVDEVTDKI 122
Score = 122 bits (295), Expect = 5e-29
Identities = 53/85 (62%), Positives = 68/85 (80%)
Frame = +2
Query: 254 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 433
R++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VV
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVV 182
Query: 434 EPYNSILTTHTTLEHSDCAFMVDNE 508
EPYNS+LTTH TL+ +DC FMVDNE
Sbjct: 183 EPYNSVLTTHATLDLADCTFMVDNE 207
Score = 93.9 bits (223), Expect = 3e-20
Identities = 45/57 (78%), Positives = 49/57 (85%)
Frame = +1
Query: 514 YDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQT*LGALTPYP 684
YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQT L PYP
Sbjct: 210 YDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQT---NLVPYP 263
Score = 44.0 bits (99), Expect = 3e-05
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +2
Query: 659 NLVP*PRIHFPLVTYAPVHLCREGYHE 739
NLVP PRIHFPLVTYAP+ + +HE
Sbjct: 258 NLVPYPRIHFPLVTYAPIVSAAKAFHE 284
Score = 41.9 bits (94), Expect = 1e-04
Identities = 22/34 (64%), Positives = 23/34 (67%)
Frame = +3
Query: 714 ISAEKATMNSFSVAEIPNACFEARPNQMVKCDPR 815
+SA KA S SV EI N CFE NQMVKCDPR
Sbjct: 276 VSAAKAFHESNSVQEITNQCFEPY-NQMVKCDPR 308
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 84.6 bits (200), Expect = 2e-17
Identities = 36/74 (48%), Positives = 50/74 (67%)
Frame = +3
Query: 27 DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 206
+ N +F+E GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+G
Sbjct: 45 ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104
Query: 207 HYTIGKEIVDLVLD 248
HYT G E+ D VLD
Sbjct: 105 HYTEGAELADAVLD 118
Score = 70.1 bits (164), Expect = 4e-13
Identities = 46/155 (29%), Positives = 72/155 (46%), Gaps = 2/155 (1%)
Frame = +2
Query: 254 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 433
R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S VV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 434 EPYNSILTTHTTLEHSDCAFMVDNEPSMTSAAVISTLSAQPTPT*IVXXXXXXXXXXXXX 613
EPYN+ L+ H +E+SD F +DNE S+ +TL + +
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNE--ALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 614 XXTAL*MWTSPSSRL--NLVP*PRIHFPLVTYAPV 712
S +L N+VP PR+HF +V +AP+
Sbjct: 239 SFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPL 273
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.0 bits (134), Expect = 2e-09
Identities = 28/80 (35%), Positives = 49/80 (61%), Gaps = 2/80 (2%)
Frame = +3
Query: 21 GDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANN 194
G D + FF ++ +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN
Sbjct: 44 GVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNN 103
Query: 195 YARGHYTIGKEIVDLVLDRI 254
+A G Y+ + I + ++D I
Sbjct: 104 WANG-YSHAERIFEDIMDMI 122
Score = 48.4 bits (110), Expect = 1e-06
Identities = 27/82 (32%), Positives = 41/82 (50%), Gaps = 1/82 (1%)
Frame = +2
Query: 263 ADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEP 439
AD L+GF + H L+ERL+ Y KK ++++P Q VS VV+P
Sbjct: 126 ADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQP 185
Query: 440 YNSILTTHTTLEHSDCAFMVDN 505
YNS+L ++D ++DN
Sbjct: 186 YNSLLALKRLTLNADSVVVLDN 207
Score = 34.3 bits (75), Expect = 0.022
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = +1
Query: 520 ICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQT*LGALTPYP 684
I L + PT+ N+L+ ++S+ T +LR+ G +N DL + +L P P
Sbjct: 213 IAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSI---IASLIPSP 264
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.029
Identities = 18/51 (35%), Positives = 29/51 (56%)
Frame = -1
Query: 660 LSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 508
L E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 168 LQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 30.3 bits (65), Expect = 0.36
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +2
Query: 368 YGKKSKLEFAIYPAPQVSTAVVE-PYNSILTTHTTLEHSDCAFMVDNEPSM 517
Y + K+E YP ++ V + PYN +L ++LE ++DN S+
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKLLCPLSSLEWVSKLILMDNANSI 160
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 27.1 bits (57), Expect = 3.3
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +3
Query: 177 EDAANNYARGHYTIGKEIVDLVLDRIASSLTSVPVCKDS*SSTPSVEV 320
ED HY+ E++DL SS S P C D +ST ++EV
Sbjct: 223 EDVIYTCPSCHYSTNSEMLDLSK---TSSDISCPNCNDQLTSTTAIEV 267
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,509,335
Number of Sequences: 5004
Number of extensions: 71457
Number of successful extensions: 216
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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