Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20912
(738 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g14920.1 68415.m01697 sulfotransferase family protein similar... 32 0.46
At5g32590.1 68418.m03867 myosin heavy chain-related similar to M... 31 0.60
At3g02120.1 68416.m00178 hydroxyproline-rich glycoprotein family... 31 1.1
At2g17200.1 68415.m01986 ubiquitin family protein weak similarit... 31 1.1
At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family... 30 1.4
At5g40950.1 68418.m04975 50S ribosomal protein L27, chloroplast,... 29 2.4
At5g13940.1 68418.m01630 hypothetical protein 29 2.4
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 29 4.3
At5g13260.1 68418.m01523 expressed protein 29 4.3
At5g12430.1 68418.m01461 DNAJ heat shock N-terminal domain-conta... 29 4.3
At5g10650.1 68418.m01233 zinc finger (C3HC4-type RING finger) fa... 29 4.3
At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger) fa... 29 4.3
At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger) fa... 29 4.3
At2g17190.1 68415.m01985 ubiquitin family protein contains INTER... 28 5.6
At2g37010.1 68415.m04539 ABC transporter family protein contains... 28 7.4
At1g64050.1 68414.m07255 expressed protein 27 9.8
>At2g14920.1 68415.m01697 sulfotransferase family protein similar to
steroid sulfotransferase from [Brassica napus]
GI:3420008, GI:3420004; contains Pfam profile PF00685:
Sulfotransferase domain
Length = 333
Score = 31.9 bits (69), Expect = 0.46
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 524 QPAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPD 637
Q +K+ ++ + + L HHNPH P + +L L+ PD
Sbjct: 91 QRSKHSLEDHQHPLLHHNPHEIVPNLELDLYLKSSKPD 128
>At5g32590.1 68418.m03867 myosin heavy chain-related similar to
Myosin heavy chain, non-muscle (Zipper protein) (Myosin
II)(SP:Q99323) {Drosophila melanogaster}
Length = 761
Score = 31.5 bits (68), Expect = 0.60
Identities = 27/83 (32%), Positives = 40/83 (48%)
Frame = +3
Query: 489 GRVSWRY*SKSSNQPRTA*SSTLMT*RITTLMPKRRNRNPNSSSKYETRIP*DERTNLST 668
GR+S R S + PR A + L + R+ L+ K+R+R+ SS P ++T T
Sbjct: 333 GRLSHRERSPHRDSPRPASRTGLSSERLADLIQKKRDRSTGDSSS-----PKRDKTRAQT 387
Query: 669 *RT*RPEPHSRIKSQGATTPKTI 737
R+ R SR S G P T+
Sbjct: 388 DRSPRLSAPSR--SMGPPPPVTV 408
>At3g02120.1 68416.m00178 hydroxyproline-rich glycoprotein family
protein
Length = 126
Score = 30.7 bits (66), Expect = 1.1
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 563 LTHHNPHAEAPKPKPELKLEIRNPDPLRRTNQPL 664
+T NP + P+P PE + +P P TN+P+
Sbjct: 21 ITTSNPESSPPRPFPESSRKHDSPPPRASTNEPM 54
>At2g17200.1 68415.m01986 ubiquitin family protein weak similarity
to PLIC-2 (ubiquitin-like type II) [Homo sapiens]
GI:9937505; contains Pfam profiles PF00240: Ubiquitin
family, PF00627: UBA/TS-N domain
Length = 551
Score = 30.7 bits (66), Expect = 1.1
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = +2
Query: 548 QYPNDLTHHNPHAEAP-KPKPELKLEIRNPDPLRRTNQPLNVKDLETRTSQSDKKSRRNH 724
QY N L NP + P+L+ ++NPD LR+ + P ++ + T Q RN
Sbjct: 405 QYMNQLMSLNPQLRSMLDSNPQLREMMQNPDFLRQFSSPEMMQQM--MTLQQSLSQNRNT 462
Query: 725 AKDD 736
A D
Sbjct: 463 ASQD 466
>At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family
protein Common family members: At5g26070, At5g19800,
At1g72790 [Arabidopsis thaliana]
Length = 575
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/81 (25%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +2
Query: 485 QRKSLLEILKQKFQPAKNRVKQY---PNDLTHHNPHAEAPKPKPELKLEIRNPDPLRRTN 655
Q S EI +++ +P + ++ + P+ P A P P P +E+ P RRT+
Sbjct: 215 QNLSKTEIEEEESEPKEIQIDTFVVKPSS-PPQQPPATPPPPPPPPPVEV--PQKPRRTH 271
Query: 656 QPLNVKDLETRTSQSDKKSRR 718
+ + +DL+ +S+ K +R
Sbjct: 272 RSVRNRDLQENAKRSETKFKR 292
>At5g40950.1 68418.m04975 50S ribosomal protein L27, chloroplast,
putative (RPL27) identical to SP|Q9FLN4 ribosomal
protein L27, chloroplast precursor {Arabidopsis
thaliana}; similar to SP|P30155 50S ribosomal protein
L27, chloroplast precursor (CL27) {Nicotiana tabacum}
Length = 198
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = +2
Query: 515 QKFQPAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDPLRRTN 655
+KF P + ++ YP ++ NP++ + + +L+ R RR N
Sbjct: 127 EKFGPDRKKISVYPREIVPENPNSYRARKRENFRLQ-REKKKARREN 172
>At5g13940.1 68418.m01630 hypothetical protein
Length = 395
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 219 DMSARRILLPGDNRCAPCNVVCNKTHHN 302
D S +LLP NR PC + THH+
Sbjct: 63 DRSYESLLLPSQNRYNPCPYLALSTHHH 90
>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related low similarity to clathrin assembly
protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
profile PF01417: ENTH domain
Length = 591
Score = 28.7 bits (61), Expect = 4.3
Identities = 17/79 (21%), Positives = 40/79 (50%), Gaps = 3/79 (3%)
Frame = +2
Query: 485 QRKSLLEILKQKFQPAKNRVKQYPNDLTHHNP---HAEAPKPKPELKLEIRNPDPLRRTN 655
Q K E +++ QP + ++ N+ T ++ E +PK E+++E P PL T+
Sbjct: 322 QEKEEEEQEQEEEQPEEPAEEENQNENTENDQPLIEEEEEEPKEEIEVEEAKPSPLIDTD 381
Query: 656 QPLNVKDLETRTSQSDKKS 712
L + ++ + ++ ++ +
Sbjct: 382 DLLGLHEINPKAAEIEQNN 400
>At5g13260.1 68418.m01523 expressed protein
Length = 537
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +2
Query: 575 NPHAEAPKPKPELKLEIRNPDPLRRTNQPLNVKDLETRTSQ 697
NP + KPKP + P + R++ P N + R SQ
Sbjct: 105 NPSSTIAKPKPVASSAVVPPPKISRSSSPANSPAVSVRASQ 145
>At5g12430.1 68418.m01461 DNAJ heat shock N-terminal
domain-containing protein similarity to
TETRATRICOPEPTIDE REPEAT PROTEIN 2 , human,
SWISSPROT:TTC2_HUMAN; contains Pfam profiles PF00226:
DnaJ domain, PF00515: TPR Domain
Length = 1165
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/53 (35%), Positives = 25/53 (47%)
Frame = -2
Query: 521 TFALISPRDSSSAYYDEN*NQYCQSHTADDQSSLSFTVYASQIVGWRGRLRTS 363
TFA S + +S Y + S + D +S SFT AS G +G L TS
Sbjct: 474 TFATASESEVTSRYKSDRKENDDHSLSNTDAASSSFTFSASSFSGVQGPLSTS 526
>At5g10650.1 68418.m01233 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 525
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/74 (25%), Positives = 30/74 (40%), Gaps = 3/74 (4%)
Frame = +1
Query: 481 YAEEESLGDIKAKVPTSQEPREAVP**LDASQPSCRSAETETRTQARNTKPGSPKTNEPT 660
+ E LG + + Q P P + S C S + + + PGSP +P+
Sbjct: 297 FGRSERLGATASSATSRQMPHPTTPTDPNPSLSFCPSNIYSSTGRVHSNMPGSPTEADPS 356
Query: 661 S---QREGLRDQNL 693
S R+GL N+
Sbjct: 357 SSLVNRDGLSHYNM 370
>At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 341
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/38 (36%), Positives = 16/38 (42%)
Frame = +2
Query: 527 PAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDP 640
P N V QYP HH P P P + L+ P P
Sbjct: 59 PNPNPVYQYPASYYHHPPPGAMPLPPYDHHLQHHPPHP 96
>At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 388
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/38 (36%), Positives = 16/38 (42%)
Frame = +2
Query: 527 PAKNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDP 640
P N V QYP HH P P P + L+ P P
Sbjct: 59 PNPNPVYQYPASYYHHPPPGAMPLPPYDHHLQHHPPHP 96
>At2g17190.1 68415.m01985 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 538
Score = 28.3 bits (60), Expect = 5.6
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 548 QYPNDLTHHNPHAEAPKP-KPELKLEIRNPDPLRRTNQPLNVKDLETRTSQSDKKSRRNH 724
QY N L NP + P+L+ ++NPD LR+ + P ++ + + QS RN
Sbjct: 389 QYMNQLMSLNPQLRSMLDMNPQLREMMQNPDFLRQFSSPEMMQQMMS-LQQSLFSQNRNT 447
Query: 725 AKDD 736
A D
Sbjct: 448 AGQD 451
>At2g37010.1 68415.m04539 ABC transporter family protein contains
ABC transporter domain, Pfam:PF00005
Length = 1063
Score = 27.9 bits (59), Expect = 7.4
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = -1
Query: 564 KSLGYCFTRFLAGWNFCFNISKRL 493
+ LGYC WN FN K L
Sbjct: 68 RDLGYCIKNLKGDWNEAFNFDKNL 91
>At1g64050.1 68414.m07255 expressed protein
Length = 668
Score = 27.5 bits (58), Expect = 9.8
Identities = 16/66 (24%), Positives = 29/66 (43%)
Frame = +2
Query: 533 KNRVKQYPNDLTHHNPHAEAPKPKPELKLEIRNPDPLRRTNQPLNVKDLETRTSQSDKKS 712
KN V++ +H+ H PKP LKL ++ + P + +E+ S
Sbjct: 580 KNHVRKDSAAEHNHHHHHHHPKPSKRLKLSTMEN---KKRSFPSSSSPIESDRKHSSSSK 636
Query: 713 RRNHAK 730
+NH++
Sbjct: 637 FKNHSR 642
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,272,983
Number of Sequences: 28952
Number of extensions: 297687
Number of successful extensions: 959
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 956
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -