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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00015
         (568 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1...    81   4e-16
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1...    81   4e-16
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1...    81   4e-16
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim...    77   1e-14
At2g29450.1 68415.m03578 glutathione S-transferase (103-1A) iden...    36   0.025
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup...    34   0.058
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim...    34   0.058
At2g29440.1 68415.m03577 glutathione S-transferase, putative           33   0.10 
At1g59700.1 68414.m06716 glutathione S-transferase, putative sim...    31   0.54 
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim...    30   0.94 
At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-...    30   0.94 
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden...    30   0.94 
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu...    29   2.2  
At4g25690.2 68417.m03699 expressed protein                             29   2.9  
At4g25690.1 68417.m03698 expressed protein                             29   2.9  
At3g09270.1 68416.m01101 glutathione S-transferase, putative sim...    29   2.9  
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide...    29   2.9  
At2g02930.1 68415.m00241 glutathione S-transferase, putative           28   5.0  
At4g02520.1 68417.m00345 glutathione S-transferase, putative           27   6.6  
At3g63070.1 68416.m07084 PWWP domain-containing protein putative...    27   6.6  
At1g69930.1 68414.m08047 glutathione S-transferase, putative sim...    27   6.6  
At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-...    27   8.7  

>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
           (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
           S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
           (Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
           {Arabidopsis thaliana}; contains Pfam profiles PF02798:
           Glutathione S-transferase, N-terminal domain and
           PF00043:Glutathione S-transferase, C-terminal domain
          Length = 228

 Score = 81.4 bits (192), Expect = 4e-16
 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%)
 Frame = +2

Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421
           L+E  P+ PL+P+D  KRA   +   ++ SGIQP QNL V+ Y+ E    E K  W  + 
Sbjct: 87  LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 146

Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508
           +T+GF A+EKLL   AGK+  GDEI LAD
Sbjct: 147 ITKGFTALEKLLVNCAGKHATGDEIYLAD 175



 Score = 40.7 bits (91), Expect = 7e-04
 Identities = 27/70 (38%), Positives = 40/70 (57%), Gaps = 6/70 (8%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGG-----EQHCNEYXAVNPMEQVPSLCIDGHTL 227
           SC+ RVRIAL LK + Y+   V+L+KG            ++  +NPM  VP+L +DG  +
Sbjct: 18  SCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL-VDGDVV 76

Query: 228 I-ESLNIMHY 254
           I +S  I+ Y
Sbjct: 77  INDSFAIIMY 86


>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
           (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
           S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
           (Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
           {Arabidopsis thaliana}; contains Pfam profiles PF02798:
           Glutathione S-transferase, N-terminal domain and
           PF00043:Glutathione S-transferase, C-terminal domain
          Length = 191

 Score = 81.4 bits (192), Expect = 4e-16
 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%)
 Frame = +2

Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421
           L+E  P+ PL+P+D  KRA   +   ++ SGIQP QNL V+ Y+ E    E K  W  + 
Sbjct: 80  LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 139

Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508
           +T+GF A+EKLL   AGK+  GDEI LAD
Sbjct: 140 ITKGFTALEKLLVNCAGKHATGDEIYLAD 168



 Score = 48.0 bits (109), Expect = 4e-06
 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239
           SC+ RVRIAL LK + Y+   V+L+K  G+Q  +++  +NPM  VP+L +DG  +I +S 
Sbjct: 18  SCAHRVRIALALKGLDYEYIPVNLLK--GDQFDSDFKKINPMGTVPAL-VDGDVVINDSF 74

Query: 240 NIMHY 254
            I+ Y
Sbjct: 75  AIIMY 79


>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
           (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
           S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
           (Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
           {Arabidopsis thaliana}; contains Pfam profiles PF02798:
           Glutathione S-transferase, N-terminal domain and
           PF00043:Glutathione S-transferase, C-terminal domain
          Length = 221

 Score = 81.4 bits (192), Expect = 4e-16
 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%)
 Frame = +2

Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421
           L+E  P+ PL+P+D  KRA   +   ++ SGIQP QNL V+ Y+ E    E K  W  + 
Sbjct: 80  LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 139

Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508
           +T+GF A+EKLL   AGK+  GDEI LAD
Sbjct: 140 ITKGFTALEKLLVNCAGKHATGDEIYLAD 168



 Score = 48.0 bits (109), Expect = 4e-06
 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239
           SC+ RVRIAL LK + Y+   V+L+K  G+Q  +++  +NPM  VP+L +DG  +I +S 
Sbjct: 18  SCAHRVRIALALKGLDYEYIPVNLLK--GDQFDSDFKKINPMGTVPAL-VDGDVVINDSF 74

Query: 240 NIMHY 254
            I+ Y
Sbjct: 75  AIIMY 79


>At2g02380.1 68415.m00176 glutathione S-transferase, putative
           similar to gi:167970 gb:AAA72320 gb:AY052332
          Length = 223

 Score = 76.6 bits (180), Expect = 1e-14
 Identities = 38/89 (42%), Positives = 54/89 (60%), Gaps = 4/89 (4%)
 Frame = +2

Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIY----VGEETKKEWSQHW 421
           L++  P+ PL+P D  KRA   +   ++ SGIQP QN+ +  Y    +  E K  W  + 
Sbjct: 83  LDDKYPEPPLLPSDYHKRAVNYQATSIVMSGIQPHQNMALFRYLEDKINAEEKTAWITNA 142

Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508
           +T+GF A+EKLL + AGKY  GDE+ LAD
Sbjct: 143 ITKGFTALEKLLVSCAGKYATGDEVYLAD 171



 Score = 48.8 bits (111), Expect = 2e-06
 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239
           SC+ RVRIAL LK + Y+   V+L+K  G+Q  +++  +NPM  VP+L +DG  +I +S 
Sbjct: 21  SCAHRVRIALTLKGLDYEYIPVNLLK--GDQSDSDFKKINPMGTVPAL-VDGDVVINDSF 77

Query: 240 NIMHY 254
            I+ Y
Sbjct: 78  AIIMY 82


>At2g29450.1 68415.m03578 glutathione S-transferase (103-1A)
           identical to Swiss-Prot:P46421 glutathione S-transferase
           103-1A [Arabidopsis thaliana]
          Length = 224

 Score = 35.5 bits (78), Expect = 0.025
 Identities = 15/38 (39%), Positives = 26/38 (68%), Gaps = 3/38 (7%)
 Frame = +2

Query: 233 IIKHHALLE---ETRPQRPLMPQDCFKRAKVREICEMI 337
           I++ H +LE   ET PQ P++PQD ++R+K R   +++
Sbjct: 66  ILESHVILEYIDETWPQNPILPQDPYERSKARFFAKLV 103


>At2g30870.1 68415.m03762 glutathione S-transferase, putative
           supported by cDNA GI:443698 GB:D17673
          Length = 215

 Score = 34.3 bits (75), Expect = 0.058
 Identities = 21/62 (33%), Positives = 31/62 (50%)
 Frame = +3

Query: 69  SWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIM 248
           S R  + L  K + ++   V L+KG  EQ   EY A+ P  ++P L    + + ES  IM
Sbjct: 13  SKRAVVTLVEKGVSFETVNVDLMKG--EQRQPEYLAIQPFGKIPVLVDGDYKIFESRAIM 70

Query: 249 HY 254
            Y
Sbjct: 71  RY 72


>At1g02940.1 68414.m00261 glutathione S-transferase, putative
           similar to glutathione S-transferase GI:860955 from
           [Hyoscyamus muticus]
          Length = 256

 Score = 34.3 bits (75), Expect = 0.058
 Identities = 23/60 (38%), Positives = 29/60 (48%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254
           RV   L+ K + YD   V+LI   G+Q    + A+NP  QVP     G  L ES  I  Y
Sbjct: 52  RVLAVLHEKGLSYDPITVNLI--AGDQKKPSFLAINPFGQVPVFLDGGLKLTESRAISEY 109


>At2g29440.1 68415.m03577 glutathione S-transferase, putative
          Length = 223

 Score = 33.5 bits (73), Expect = 0.10
 Identities = 15/38 (39%), Positives = 25/38 (65%), Gaps = 3/38 (7%)
 Frame = +2

Query: 233 IIKHHALLE---ETRPQRPLMPQDCFKRAKVREICEMI 337
           II+ H +LE   ET    P++PQD F+R+K R + +++
Sbjct: 66  IIESHVILEYIDETWKHNPILPQDPFQRSKARVLAKLV 103


>At1g59700.1 68414.m06716 glutathione S-transferase, putative
           similar to glutathione S-transferase GB:AAF29773
           GI:6856103 from [Gossypium hirsutum]
          Length = 234

 Score = 31.1 bits (67), Expect = 0.54
 Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPM-EQVPSLCIDGHTLIESLNIMH 251
           R +IAL LK + YD    +L     E         NP+ ++VP L  +   ++ESLNI+ 
Sbjct: 20  RPKIALRLKSVDYDYVEENLFGSKSELLLKS----NPVHKKVPVLLHNNKPIVESLNIVE 75

Query: 252 Y 254
           Y
Sbjct: 76  Y 76


>At5g41240.1 68418.m05011 glutathione S-transferase, putative
           similar to glutathione S-transferase, GST 10b
           GB:CAA10662 [Arabidopsis thaliana] 37349.
          Length = 591

 Score = 30.3 bits (65), Expect = 0.94
 Identities = 22/60 (36%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
 Frame = +3

Query: 78  VRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGH-TLIESLNIMHY 254
           V I   + EI +D   +SL  G  +Q   E+  +NPM +VP++ +DG   L ES  I+ Y
Sbjct: 17  VLIFCKVNEIQFDEILISL--GKRQQLSPEFKEINPMGKVPAI-VDGRLKLFESHAILIY 73


>At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-1
           [Zea mays] EMBL:X58573
          Length = 237

 Score = 30.3 bits (65), Expect = 0.94
 Identities = 23/71 (32%), Positives = 37/71 (52%), Gaps = 2/71 (2%)
 Frame = +3

Query: 48  LILAHSCSW--RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGH 221
           L ++++C +  RV I  NLK +  +IK V +          E   VNP  +VP+L  +G 
Sbjct: 33  LYISYTCPFAQRVWITRNLKGLQDEIKLVPIDLPNRPAWLKE--KVNPANKVPALEHNGK 90

Query: 222 TLIESLNIMHY 254
              ESL+++ Y
Sbjct: 91  ITGESLDLIKY 101


>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
           identical to GB:X95295. Based on identical cDNA hits,
           the translation is now 40 AAs longer at the N-terminal,
           and start of exon2 is also corrected.
          Length = 263

 Score = 30.3 bits (65), Expect = 0.94
 Identities = 19/64 (29%), Positives = 32/64 (50%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLN 242
           + + RV   L  K++ +++  V +  G  +Q    + A+NP  Q+P+L     TL ES  
Sbjct: 61  TATMRVLATLYEKDLQFELIPVDMRAGAHKQEA--HLALNPFGQIPALEDGDLTLFESRA 118

Query: 243 IMHY 254
           I  Y
Sbjct: 119 ITQY 122


>At3g62760.1 68416.m07050 glutathione S-transferase, putative
           Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
          Length = 219

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 19/64 (29%), Positives = 31/64 (48%)
 Frame = +3

Query: 63  SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLN 242
           +C  RV + L+ K   +++  V+L           + ++NP  +VP+L  D  TL ES  
Sbjct: 12  ACVARVLLCLHEKNTEFELVPVNLF--ACHHKLPSFLSMNPFGKVPALQDDDLTLFESRA 69

Query: 243 IMHY 254
           I  Y
Sbjct: 70  ITAY 73


>At4g25690.2 68417.m03699 expressed protein 
          Length = 191

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = -2

Query: 459 DSNFSIALKPLVTQCWDHSFFVSS 388
           DSN+S++ KP +   WD++  +S+
Sbjct: 138 DSNWSVSYKPFMKDVWDNNMVISA 161


>At4g25690.1 68417.m03698 expressed protein 
          Length = 191

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = -2

Query: 459 DSNFSIALKPLVTQCWDHSFFVSS 388
           DSN+S++ KP +   WD++  +S+
Sbjct: 138 DSNWSVSYKPFMKDVWDNNMVISA 161


>At3g09270.1 68416.m01101 glutathione S-transferase, putative
           similar to glutathione transferase GB:CAA71784 [Glycine
           max]
          Length = 224

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 20/62 (32%), Positives = 32/62 (51%)
 Frame = +3

Query: 69  SWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIM 248
           S RV + L LK IPY+      + G       +Y  ++  ++VP L  +G ++ ESL I+
Sbjct: 18  SKRVEMVLKLKGIPYEYIEED-VYGNRSPMLLKYNPIH--KKVPVLIHNGRSIAESLVIV 74

Query: 249 HY 254
            Y
Sbjct: 75  EY 76


>At2g30860.1 68415.m03761 glutathione S-transferase, putative
           identical to GB:Y12295
          Length = 215

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 17/60 (28%), Positives = 29/60 (48%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254
           R  + L  K + ++   V L+KG  +Q    Y A+ P   VP++    + + ES  +M Y
Sbjct: 15  RALVTLIEKGVAFETIPVDLMKGEHKQPA--YLALQPFGTVPAVVDGDYKIFESRAVMRY 72


>At2g02930.1 68415.m00241 glutathione S-transferase, putative
          Length = 212

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 21/66 (31%), Positives = 31/66 (46%)
 Frame = +3

Query: 57  AHSCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIES 236
           A + + RV IAL+ K + +++  V L    GE     + + NP  QVP+       L ES
Sbjct: 11  ASTSTRRVLIALHEKNLDFELVHVEL--KDGEHKKEPFLSRNPFGQVPAFEDGDLKLFES 68

Query: 237 LNIMHY 254
             I  Y
Sbjct: 69  RAITQY 74


>At4g02520.1 68417.m00345 glutathione S-transferase, putative
          Length = 212

 Score = 27.5 bits (58), Expect = 6.6
 Identities = 20/60 (33%), Positives = 28/60 (46%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254
           RV IAL+ K + +++  V L    GE     + + NP  QVP+       L ES  I  Y
Sbjct: 17  RVLIALHEKNLDFELVHVEL--KDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQY 74


>At3g63070.1 68416.m07084 PWWP domain-containing protein putative
           transcription factor HUA2, Arabidopsis thaliana,
           EMBL:AF116556
          Length = 1347

 Score = 27.5 bits (58), Expect = 6.6
 Identities = 9/17 (52%), Positives = 14/17 (82%)
 Frame = -3

Query: 407 IPSLSPHQHKLAQSDSA 357
           +P+ SPHQHK+ + DS+
Sbjct: 615 VPAQSPHQHKIQEYDSS 631


>At1g69930.1 68414.m08047 glutathione S-transferase, putative
           similar to glutathione transferase GB:CAA09188
           [Alopecurus myosuroides]
          Length = 234

 Score = 27.5 bits (58), Expect = 6.6
 Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPM-EQVPSLCIDGHTLIESLNIMH 251
           R RIALNLK + Y+      ++             NP+ +Q+P L      + ESLNI+ 
Sbjct: 26  RTRIALNLKNVAYE-----YLEEEDTLSSESVLNYNPVHKQIPILIHGNKPIRESLNIVM 80

Query: 252 Y 254
           Y
Sbjct: 81  Y 81


>At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-1,
           Zea mays, EMBL:X58573
          Length = 235

 Score = 27.1 bits (57), Expect = 8.7
 Identities = 21/60 (35%), Positives = 29/60 (48%)
 Frame = +3

Query: 75  RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254
           RV I  N K +   IK V L  G       E   V P  +VP+L  +G  + ESL+++ Y
Sbjct: 42  RVWITRNFKGLQEKIKLVPLDLGNRPAWYKE--KVYPENKVPALEHNGKIIGESLDLIKY 99


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,299,801
Number of Sequences: 28952
Number of extensions: 243375
Number of successful extensions: 503
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 484
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 494
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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