BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00015 (568 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 81 4e-16 At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 81 4e-16 At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 81 4e-16 At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 77 1e-14 At2g29450.1 68415.m03578 glutathione S-transferase (103-1A) iden... 36 0.025 At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 34 0.058 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 34 0.058 At2g29440.1 68415.m03577 glutathione S-transferase, putative 33 0.10 At1g59700.1 68414.m06716 glutathione S-transferase, putative sim... 31 0.54 At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 30 0.94 At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-... 30 0.94 At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 0.94 At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 29 2.2 At4g25690.2 68417.m03699 expressed protein 29 2.9 At4g25690.1 68417.m03698 expressed protein 29 2.9 At3g09270.1 68416.m01101 glutathione S-transferase, putative sim... 29 2.9 At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 29 2.9 At2g02930.1 68415.m00241 glutathione S-transferase, putative 28 5.0 At4g02520.1 68417.m00345 glutathione S-transferase, putative 27 6.6 At3g63070.1 68416.m07084 PWWP domain-containing protein putative... 27 6.6 At1g69930.1 68414.m08047 glutathione S-transferase, putative sim... 27 6.6 At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-... 27 8.7 >At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 228 Score = 81.4 bits (192), Expect = 4e-16 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%) Frame = +2 Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421 L+E P+ PL+P+D KRA + ++ SGIQP QNL V+ Y+ E E K W + Sbjct: 87 LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 146 Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508 +T+GF A+EKLL AGK+ GDEI LAD Sbjct: 147 ITKGFTALEKLLVNCAGKHATGDEIYLAD 175 Score = 40.7 bits (91), Expect = 7e-04 Identities = 27/70 (38%), Positives = 40/70 (57%), Gaps = 6/70 (8%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGG-----EQHCNEYXAVNPMEQVPSLCIDGHTL 227 SC+ RVRIAL LK + Y+ V+L+KG ++ +NPM VP+L +DG + Sbjct: 18 SCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL-VDGDVV 76 Query: 228 I-ESLNIMHY 254 I +S I+ Y Sbjct: 77 INDSFAIIMY 86 >At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 191 Score = 81.4 bits (192), Expect = 4e-16 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%) Frame = +2 Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421 L+E P+ PL+P+D KRA + ++ SGIQP QNL V+ Y+ E E K W + Sbjct: 80 LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 139 Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508 +T+GF A+EKLL AGK+ GDEI LAD Sbjct: 140 ITKGFTALEKLLVNCAGKHATGDEIYLAD 168 Score = 48.0 bits (109), Expect = 4e-06 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239 SC+ RVRIAL LK + Y+ V+L+K G+Q +++ +NPM VP+L +DG +I +S Sbjct: 18 SCAHRVRIALALKGLDYEYIPVNLLK--GDQFDSDFKKINPMGTVPAL-VDGDVVINDSF 74 Query: 240 NIMHY 254 I+ Y Sbjct: 75 AIIMY 79 >At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 221 Score = 81.4 bits (192), Expect = 4e-16 Identities = 42/89 (47%), Positives = 56/89 (62%), Gaps = 4/89 (4%) Frame = +2 Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIYVGE----ETKKEWSQHW 421 L+E P+ PL+P+D KRA + ++ SGIQP QNL V+ Y+ E E K W + Sbjct: 80 LDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRYIEEKINVEEKTAWVNNA 139 Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508 +T+GF A+EKLL AGK+ GDEI LAD Sbjct: 140 ITKGFTALEKLLVNCAGKHATGDEIYLAD 168 Score = 48.0 bits (109), Expect = 4e-06 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239 SC+ RVRIAL LK + Y+ V+L+K G+Q +++ +NPM VP+L +DG +I +S Sbjct: 18 SCAHRVRIALALKGLDYEYIPVNLLK--GDQFDSDFKKINPMGTVPAL-VDGDVVINDSF 74 Query: 240 NIMHY 254 I+ Y Sbjct: 75 AIIMY 79 >At2g02380.1 68415.m00176 glutathione S-transferase, putative similar to gi:167970 gb:AAA72320 gb:AY052332 Length = 223 Score = 76.6 bits (180), Expect = 1e-14 Identities = 38/89 (42%), Positives = 54/89 (60%), Gaps = 4/89 (4%) Frame = +2 Query: 254 LEETRPQRPLMPQDCFKRAKVREICEMIASGIQPLQNLIVLIY----VGEETKKEWSQHW 421 L++ P+ PL+P D KRA + ++ SGIQP QN+ + Y + E K W + Sbjct: 83 LDDKYPEPPLLPSDYHKRAVNYQATSIVMSGIQPHQNMALFRYLEDKINAEEKTAWITNA 142 Query: 422 VTRGFRAIEKLLSTTAGKYCVGDEITLAD 508 +T+GF A+EKLL + AGKY GDE+ LAD Sbjct: 143 ITKGFTALEKLLVSCAGKYATGDEVYLAD 171 Score = 48.8 bits (111), Expect = 2e-06 Identities = 28/65 (43%), Positives = 43/65 (66%), Gaps = 1/65 (1%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLI-ESL 239 SC+ RVRIAL LK + Y+ V+L+K G+Q +++ +NPM VP+L +DG +I +S Sbjct: 21 SCAHRVRIALTLKGLDYEYIPVNLLK--GDQSDSDFKKINPMGTVPAL-VDGDVVINDSF 77 Query: 240 NIMHY 254 I+ Y Sbjct: 78 AIIMY 82 >At2g29450.1 68415.m03578 glutathione S-transferase (103-1A) identical to Swiss-Prot:P46421 glutathione S-transferase 103-1A [Arabidopsis thaliana] Length = 224 Score = 35.5 bits (78), Expect = 0.025 Identities = 15/38 (39%), Positives = 26/38 (68%), Gaps = 3/38 (7%) Frame = +2 Query: 233 IIKHHALLE---ETRPQRPLMPQDCFKRAKVREICEMI 337 I++ H +LE ET PQ P++PQD ++R+K R +++ Sbjct: 66 ILESHVILEYIDETWPQNPILPQDPYERSKARFFAKLV 103 >At2g30870.1 68415.m03762 glutathione S-transferase, putative supported by cDNA GI:443698 GB:D17673 Length = 215 Score = 34.3 bits (75), Expect = 0.058 Identities = 21/62 (33%), Positives = 31/62 (50%) Frame = +3 Query: 69 SWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIM 248 S R + L K + ++ V L+KG EQ EY A+ P ++P L + + ES IM Sbjct: 13 SKRAVVTLVEKGVSFETVNVDLMKG--EQRQPEYLAIQPFGKIPVLVDGDYKIFESRAIM 70 Query: 249 HY 254 Y Sbjct: 71 RY 72 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 34.3 bits (75), Expect = 0.058 Identities = 23/60 (38%), Positives = 29/60 (48%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254 RV L+ K + YD V+LI G+Q + A+NP QVP G L ES I Y Sbjct: 52 RVLAVLHEKGLSYDPITVNLI--AGDQKKPSFLAINPFGQVPVFLDGGLKLTESRAISEY 109 >At2g29440.1 68415.m03577 glutathione S-transferase, putative Length = 223 Score = 33.5 bits (73), Expect = 0.10 Identities = 15/38 (39%), Positives = 25/38 (65%), Gaps = 3/38 (7%) Frame = +2 Query: 233 IIKHHALLE---ETRPQRPLMPQDCFKRAKVREICEMI 337 II+ H +LE ET P++PQD F+R+K R + +++ Sbjct: 66 IIESHVILEYIDETWKHNPILPQDPFQRSKARVLAKLV 103 >At1g59700.1 68414.m06716 glutathione S-transferase, putative similar to glutathione S-transferase GB:AAF29773 GI:6856103 from [Gossypium hirsutum] Length = 234 Score = 31.1 bits (67), Expect = 0.54 Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPM-EQVPSLCIDGHTLIESLNIMH 251 R +IAL LK + YD +L E NP+ ++VP L + ++ESLNI+ Sbjct: 20 RPKIALRLKSVDYDYVEENLFGSKSELLLKS----NPVHKKVPVLLHNNKPIVESLNIVE 75 Query: 252 Y 254 Y Sbjct: 76 Y 76 >At5g41240.1 68418.m05011 glutathione S-transferase, putative similar to glutathione S-transferase, GST 10b GB:CAA10662 [Arabidopsis thaliana] 37349. Length = 591 Score = 30.3 bits (65), Expect = 0.94 Identities = 22/60 (36%), Positives = 33/60 (55%), Gaps = 1/60 (1%) Frame = +3 Query: 78 VRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGH-TLIESLNIMHY 254 V I + EI +D +SL G +Q E+ +NPM +VP++ +DG L ES I+ Y Sbjct: 17 VLIFCKVNEIQFDEILISL--GKRQQLSPEFKEINPMGKVPAI-VDGRLKLFESHAILIY 73 >At5g02780.1 68418.m00220 In2-1 protein, putative similar to In2-1 [Zea mays] EMBL:X58573 Length = 237 Score = 30.3 bits (65), Expect = 0.94 Identities = 23/71 (32%), Positives = 37/71 (52%), Gaps = 2/71 (2%) Frame = +3 Query: 48 LILAHSCSW--RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGH 221 L ++++C + RV I NLK + +IK V + E VNP +VP+L +G Sbjct: 33 LYISYTCPFAQRVWITRNLKGLQDEIKLVPIDLPNRPAWLKE--KVNPANKVPALEHNGK 90 Query: 222 TLIESLNIMHY 254 ESL+++ Y Sbjct: 91 ITGESLDLIKY 101 >At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) identical to GB:X95295. Based on identical cDNA hits, the translation is now 40 AAs longer at the N-terminal, and start of exon2 is also corrected. Length = 263 Score = 30.3 bits (65), Expect = 0.94 Identities = 19/64 (29%), Positives = 32/64 (50%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLN 242 + + RV L K++ +++ V + G +Q + A+NP Q+P+L TL ES Sbjct: 61 TATMRVLATLYEKDLQFELIPVDMRAGAHKQEA--HLALNPFGQIPALEDGDLTLFESRA 118 Query: 243 IMHY 254 I Y Sbjct: 119 ITQY 122 >At3g62760.1 68416.m07050 glutathione S-transferase, putative Glutathione transferase III(b) - Zea mays, EMBL:AJ010296 Length = 219 Score = 29.1 bits (62), Expect = 2.2 Identities = 19/64 (29%), Positives = 31/64 (48%) Frame = +3 Query: 63 SCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLN 242 +C RV + L+ K +++ V+L + ++NP +VP+L D TL ES Sbjct: 12 ACVARVLLCLHEKNTEFELVPVNLF--ACHHKLPSFLSMNPFGKVPALQDDDLTLFESRA 69 Query: 243 IMHY 254 I Y Sbjct: 70 ITAY 73 >At4g25690.2 68417.m03699 expressed protein Length = 191 Score = 28.7 bits (61), Expect = 2.9 Identities = 9/24 (37%), Positives = 17/24 (70%) Frame = -2 Query: 459 DSNFSIALKPLVTQCWDHSFFVSS 388 DSN+S++ KP + WD++ +S+ Sbjct: 138 DSNWSVSYKPFMKDVWDNNMVISA 161 >At4g25690.1 68417.m03698 expressed protein Length = 191 Score = 28.7 bits (61), Expect = 2.9 Identities = 9/24 (37%), Positives = 17/24 (70%) Frame = -2 Query: 459 DSNFSIALKPLVTQCWDHSFFVSS 388 DSN+S++ KP + WD++ +S+ Sbjct: 138 DSNWSVSYKPFMKDVWDNNMVISA 161 >At3g09270.1 68416.m01101 glutathione S-transferase, putative similar to glutathione transferase GB:CAA71784 [Glycine max] Length = 224 Score = 28.7 bits (61), Expect = 2.9 Identities = 20/62 (32%), Positives = 32/62 (51%) Frame = +3 Query: 69 SWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIM 248 S RV + L LK IPY+ + G +Y ++ ++VP L +G ++ ESL I+ Sbjct: 18 SKRVEMVLKLKGIPYEYIEED-VYGNRSPMLLKYNPIH--KKVPVLIHNGRSIAESLVIV 74 Query: 249 HY 254 Y Sbjct: 75 EY 76 >At2g30860.1 68415.m03761 glutathione S-transferase, putative identical to GB:Y12295 Length = 215 Score = 28.7 bits (61), Expect = 2.9 Identities = 17/60 (28%), Positives = 29/60 (48%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254 R + L K + ++ V L+KG +Q Y A+ P VP++ + + ES +M Y Sbjct: 15 RALVTLIEKGVAFETIPVDLMKGEHKQPA--YLALQPFGTVPAVVDGDYKIFESRAVMRY 72 >At2g02930.1 68415.m00241 glutathione S-transferase, putative Length = 212 Score = 27.9 bits (59), Expect = 5.0 Identities = 21/66 (31%), Positives = 31/66 (46%) Frame = +3 Query: 57 AHSCSWRVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIES 236 A + + RV IAL+ K + +++ V L GE + + NP QVP+ L ES Sbjct: 11 ASTSTRRVLIALHEKNLDFELVHVEL--KDGEHKKEPFLSRNPFGQVPAFEDGDLKLFES 68 Query: 237 LNIMHY 254 I Y Sbjct: 69 RAITQY 74 >At4g02520.1 68417.m00345 glutathione S-transferase, putative Length = 212 Score = 27.5 bits (58), Expect = 6.6 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254 RV IAL+ K + +++ V L GE + + NP QVP+ L ES I Y Sbjct: 17 RVLIALHEKNLDFELVHVEL--KDGEHKKEPFLSRNPFGQVPAFEDGDLKLFESRAITQY 74 >At3g63070.1 68416.m07084 PWWP domain-containing protein putative transcription factor HUA2, Arabidopsis thaliana, EMBL:AF116556 Length = 1347 Score = 27.5 bits (58), Expect = 6.6 Identities = 9/17 (52%), Positives = 14/17 (82%) Frame = -3 Query: 407 IPSLSPHQHKLAQSDSA 357 +P+ SPHQHK+ + DS+ Sbjct: 615 VPAQSPHQHKIQEYDSS 631 >At1g69930.1 68414.m08047 glutathione S-transferase, putative similar to glutathione transferase GB:CAA09188 [Alopecurus myosuroides] Length = 234 Score = 27.5 bits (58), Expect = 6.6 Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPM-EQVPSLCIDGHTLIESLNIMH 251 R RIALNLK + Y+ ++ NP+ +Q+P L + ESLNI+ Sbjct: 26 RTRIALNLKNVAYE-----YLEEEDTLSSESVLNYNPVHKQIPILIHGNKPIRESLNIVM 80 Query: 252 Y 254 Y Sbjct: 81 Y 81 >At5g02790.1 68418.m00221 In2-1 protein, putative similar to In2-1, Zea mays, EMBL:X58573 Length = 235 Score = 27.1 bits (57), Expect = 8.7 Identities = 21/60 (35%), Positives = 29/60 (48%) Frame = +3 Query: 75 RVRIALNLKEIPYDIKAVSLIKGGGEQHCNEYXAVNPMEQVPSLCIDGHTLIESLNIMHY 254 RV I N K + IK V L G E V P +VP+L +G + ESL+++ Y Sbjct: 42 RVWITRNFKGLQEKIKLVPLDLGNRPAWYKE--KVYPENKVPALEHNGKIIGESLDLIKY 99 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,299,801 Number of Sequences: 28952 Number of extensions: 243375 Number of successful extensions: 503 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 484 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 494 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1092379416 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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