BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS312G12f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g50010.1 68414.m05612 tubulin alpha-2/alpha-4 chain (TUA2) id... 27 7.7 At1g04820.1 68414.m00478 tubulin alpha-2/alpha-4 chain (TUA4) ne... 27 7.7 >At1g50010.1 68414.m05612 tubulin alpha-2/alpha-4 chain (TUA2) identical to tubulin alpha-2/alpha-4 chain SP|P29510 GB:P29510 from [Arabidopsis thaliana] Length = 450 Score = 27.1 bits (57), Expect = 7.7 Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 2/62 (3%) Frame = -2 Query: 184 LSLTVTRDPSY-VRKRSLTI*FPVYLHLINVKISTIKLITAAFQYETHTDQPV-PLQTSL 11 +S+ + + Y + +RSL+I P Y +L + I +TA+ +++ + V QT+L Sbjct: 200 VSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNL 259 Query: 10 VP 5 VP Sbjct: 260 VP 261 >At1g04820.1 68414.m00478 tubulin alpha-2/alpha-4 chain (TUA4) nearly identical to SP:P29510 Tubulin alpha-2/alpha-4 chain from [Arabidopsis thaliana] Length = 450 Score = 27.1 bits (57), Expect = 7.7 Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 2/62 (3%) Frame = -2 Query: 184 LSLTVTRDPSY-VRKRSLTI*FPVYLHLINVKISTIKLITAAFQYETHTDQPV-PLQTSL 11 +S+ + + Y + +RSL+I P Y +L + I +TA+ +++ + V QT+L Sbjct: 200 VSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNL 259 Query: 10 VP 5 VP Sbjct: 260 VP 261 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,881,398 Number of Sequences: 28952 Number of extensions: 176262 Number of successful extensions: 337 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 333 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 337 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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