Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30600
(747 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g04160.1 68415.m00400 subtilisin-like protease (AIR3) almost ... 31 0.81
At5g18230.1 68418.m02139 transcription regulator NOT2/NOT3/NOT5 ... 31 1.1
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 31 1.1
At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase ... 31 1.1
At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase ... 31 1.1
At1g58220.1 68414.m06612 myb family transcription factor contain... 29 2.5
At3g15970.1 68416.m02019 Ran-binding protein 1 domain-containing... 29 3.3
At2g36560.1 68415.m04484 DNA-binding protein-related contains Pf... 29 3.3
At3g14090.1 68416.m01781 exocyst subunit EXO70 family protein c... 28 7.6
At5g42950.1 68418.m05236 GYF domain-containing protein contains ... 27 10.0
At3g20280.2 68416.m02570 PHD finger family protein contains Pfam... 27 10.0
At3g20280.1 68416.m02569 PHD finger family protein contains Pfam... 27 10.0
At3g18310.1 68416.m02330 expressed protein 27 10.0
At3g05610.1 68416.m00623 pectinesterase family protein contains ... 27 10.0
>At2g04160.1 68415.m00400 subtilisin-like protease (AIR3) almost
identical to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana], missing 200 aa at
N-terminus
Length = 772
Score = 31.1 bits (67), Expect = 0.81
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = -2
Query: 662 DVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESS-TGASVTEVINDEVSVGSK 486
DVI S G T ND V++GS VVV S ++V+ V +++VG+
Sbjct: 291 DVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAS 350
Query: 485 TI 480
T+
Sbjct: 351 TM 352
>At5g18230.1 68418.m02139 transcription regulator NOT2/NOT3/NOT5
family protein contains Pfam domain PF04153: NOT2 / NOT3
/ NOT5 family
Length = 843
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +1
Query: 598 PTDTSSLITSVALAPVEDSITSFTTTAGAATIVLEPTDTSS 720
P D++S+ S A+ +DS F T +G + +L+P SS
Sbjct: 602 PDDSASIAASKAIQSEDDSKVLFDTPSGMPSYMLDPVQVSS 642
Score = 29.1 bits (62), Expect = 3.3
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +1
Query: 493 PTDTSSLITSVTLAPVEDSITSFTTTAGAATIVLEPTDTSS 615
P D++S+ S + +DS F T +G + +L+P SS
Sbjct: 602 PDDSASIAASKAIQSEDDSKVLFDTPSGMPSYMLDPVQVSS 642
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 30.7 bits (66), Expect = 1.1
Identities = 25/112 (22%), Positives = 42/112 (37%)
Frame = +1
Query: 412 TSVALAPVEDSIGSFTTTAGAATIVLEPTDTSSLITSVTLAPVEDSITSFTTTAGAATIV 591
TS A +P S + + + PT S TS + +P S + + +
Sbjct: 1590 TSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPA 1649
Query: 592 LEPTDTSSLITSVALAPVEDSITSFTTTAGAATIVLEPTDTSSLITSVALAP 747
PT + TS + +P S + + + + PT S TS A +P
Sbjct: 1650 YSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSP 1701
>At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 30.7 bits (66), Expect = 1.1
Identities = 34/163 (20%), Positives = 66/163 (40%), Gaps = 2/163 (1%)
Frame = -2
Query: 710 SVGSKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESS-TG 534
S S + + A V + IE AS ++++E+ KT+VA+ + V ESS TG
Sbjct: 16 SSSSSSSSSGRASVKIEEIEGGAAASGVVIVSEELETNPKTVVAS--IADETVAESSGTG 73
Query: 533 ASVTEVINDEVSVGSKTIVAAPAVVVNDP-IESSTGASATEVINDEALVSSKXXXXXXXX 357
+ GS + A + N P ++ S +V + A ++K
Sbjct: 74 NKSFSRVWTMPLEGSSSSDKAESSSTNQPRLDKSKTERQQKVTHILAEDAAKIFDDKISA 133
Query: 356 XXXXXXXXXVLNTEAPQDLSTKILADAVPEAVIVTAPEAETAI 228
+ + + ++ ADA+P+ ++V E++ +
Sbjct: 134 GKKLKLLNRIATVKHDGTVEFEVPADAIPQPIVVDRGESKNGV 176
>At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 30.7 bits (66), Expect = 1.1
Identities = 34/163 (20%), Positives = 66/163 (40%), Gaps = 2/163 (1%)
Frame = -2
Query: 710 SVGSKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESS-TG 534
S S + + A V + IE AS ++++E+ KT+VA+ + V ESS TG
Sbjct: 16 SSSSSSSSSGRASVKIEEIEGGAAASGVVIVSEELETNPKTVVAS--IADETVAESSGTG 73
Query: 533 ASVTEVINDEVSVGSKTIVAAPAVVVNDP-IESSTGASATEVINDEALVSSKXXXXXXXX 357
+ GS + A + N P ++ S +V + A ++K
Sbjct: 74 NKSFSRVWTMPLEGSSSSDKAESSSTNQPRLDKSKTERQQKVTHILAEDAAKIFDDKISA 133
Query: 356 XXXXXXXXXVLNTEAPQDLSTKILADAVPEAVIVTAPEAETAI 228
+ + + ++ ADA+P+ ++V E++ +
Sbjct: 134 GKKLKLLNRIATVKHDGTVEFEVPADAIPQPIVVDRGESKNGV 176
>At1g58220.1 68414.m06612 myb family transcription factor contains
Pfam profile: PF00249: Myb-like DNA-binding domain
Length = 834
Score = 29.5 bits (63), Expect = 2.5
Identities = 24/72 (33%), Positives = 31/72 (43%)
Frame = -2
Query: 683 APAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESSTGASVTEVINDE 504
APA V + S A+AT V + SVG+ V P + S GA+ V +
Sbjct: 651 APAATVVTPQKPSVVAAATVVTPQKPSVGAAATVVTPQ-------KPSVGAAANVVTPQK 703
Query: 503 VSVGSKTIVAAP 468
SVGS V P
Sbjct: 704 PSVGSAATVVTP 715
>At3g15970.1 68416.m02019 Ran-binding protein 1 domain-containing
protein / RanBP1 domain-containing protein similar to
Ran binding protein [Homo sapiens] GI:624232; contains
Pfam profile PF00638: RanBP1 domain
Length = 465
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/73 (23%), Positives = 29/73 (39%)
Frame = -2
Query: 626 EVINDEVSVGSKTIVAAPAVVVNDVIESSTGASVTEVINDEVSVGSKTIVAAPAVVVNDP 447
E DE+S +K I+ +++ + S G DE+ T VAA V D
Sbjct: 135 ETQGDEISAKTKDIIDGGEKEMSEAVNSVEGGGAVNKNEDEIKTTMVTEVAAGEETVKDD 194
Query: 446 IESSTGASATEVI 408
+S ++ +
Sbjct: 195 NNNSNTVEGSDCV 207
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/59 (28%), Positives = 25/59 (42%)
Frame = -2
Query: 731 EVINDEVSVGSKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVND 555
E DE+S +K I+ +++ + S G A DE+ T VAA V D
Sbjct: 135 ETQGDEISAKTKDIIDGGEKEMSEAVNSVEGGGAVNKNEDEIKTTMVTEVAAGEETVKD 193
>At2g36560.1 68415.m04484 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 574
Score = 29.1 bits (62), Expect = 3.3
Identities = 23/54 (42%), Positives = 30/54 (55%)
Frame = -2
Query: 701 SKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESS 540
+KT VAAPA VV E A++T + V +KT AAPA VV V ++S
Sbjct: 14 TKTAVAAPATVV----EGVNDANSTPISPFPV---TKTAEAAPATVVEGVNDAS 60
>At3g14090.1 68416.m01781 exocyst subunit EXO70 family protein
contains Pfam domain PF03081: Exo70 exocyst complex
subunit;
Length = 623
Score = 27.9 bits (59), Expect = 7.6
Identities = 21/72 (29%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = +1
Query: 526 TLAPVEDSITSFTTTAGAATIVLEPTDTSSLITSVALAPVEDS----ITSFTTTAGAATI 693
+L+ V S +S + AAT+V E ++ +A+A +ED + S TTT ++
Sbjct: 59 SLSSVSFSSSSSSAATSAATVVDEHEVKANSAIQIAMARLEDEFRNILLSHTTTFEPDSL 118
Query: 694 VLEPTDTSSLIT 729
LE S +T
Sbjct: 119 FLEEPSVSPSLT 130
>At5g42950.1 68418.m05236 GYF domain-containing protein contains Pfam
profile: PF02213 GYF domain
Length = 1714
Score = 27.5 bits (58), Expect = 10.0
Identities = 32/99 (32%), Positives = 47/99 (47%), Gaps = 13/99 (13%)
Frame = -2
Query: 656 IESSTGASATEVINDEVSVGSKTIVAAP----AVVVNDVIESSTGASVTEVIN------D 507
IE S ATEV N++VSV KT A D+ +S++ A + E D
Sbjct: 1081 IELSGETPATEVKNNDVSVARKTSEKKSRKQRAKQAADLAKSTSRAPLQETKKPQPGSAD 1140
Query: 506 EVSVGSKTIVAAPAVVVNDP--IESSTG-ASATEVINDE 399
+ + KT +A ++ ND I+SST AS T ++ E
Sbjct: 1141 DSEIKGKTKKSADTLIDNDTHLIKSSTATASNTSQMSSE 1179
>At3g20280.2 68416.m02570 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 482
Score = 27.5 bits (58), Expect = 10.0
Identities = 24/86 (27%), Positives = 39/86 (45%)
Frame = -2
Query: 707 VGSKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESSTGAS 528
V T AAP V++ + S S + +ND+ + + A V ++ ++ AS
Sbjct: 326 VAKATEDAAPLENVSECEKPSESTSHPDSLNDKTISENVQESSKDAKVDSEACQNHPTAS 385
Query: 527 VTEVINDEVSVGSKTIVAAPAVVVND 450
V+ D+ S TI AAP+V D
Sbjct: 386 PATVVPDQDS----TITAAPSVTQED 407
>At3g20280.1 68416.m02569 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 743
Score = 27.5 bits (58), Expect = 10.0
Identities = 24/86 (27%), Positives = 39/86 (45%)
Frame = -2
Query: 707 VGSKTIVAAPAVVVNDVIESSTGASATEVINDEVSVGSKTIVAAPAVVVNDVIESSTGAS 528
V T AAP V++ + S S + +ND+ + + A V ++ ++ AS
Sbjct: 587 VAKATEDAAPLENVSECEKPSESTSHPDSLNDKTISENVQESSKDAKVDSEACQNHPTAS 646
Query: 527 VTEVINDEVSVGSKTIVAAPAVVVND 450
V+ D+ S TI AAP+V D
Sbjct: 647 PATVVPDQDS----TITAAPSVTQED 668
>At3g18310.1 68416.m02330 expressed protein
Length = 873
Score = 27.5 bits (58), Expect = 10.0
Identities = 17/57 (29%), Positives = 35/57 (61%), Gaps = 2/57 (3%)
Frame = +1
Query: 493 PTDTSSLITSVTLAPVEDSITSFTTTAGAATIV-LEPTDTSSLITSVALA-PVEDSI 657
P+D+ L +S +L+P SIT T A ++ + P+D+S++ +S ++ P +D++
Sbjct: 41 PSDSQLLFSSPSLSPPILSITPHLTPARFLSVSGVPPSDSSAINSSFKISNPHDDTV 97
>At3g05610.1 68416.m00623 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 669
Score = 27.5 bits (58), Expect = 10.0
Identities = 25/114 (21%), Positives = 53/114 (46%)
Frame = +1
Query: 394 NASSLITSVALAPVEDSIGSFTTTAGAATIVLEPTDTSSLITSVTLAPVEDSITSFTTTA 573
N ++ T+ +++ +FT T+GA +I + +S +S+++A +T TA
Sbjct: 569 NPAAATTTPSVSAAAPGFSTFTDTSGADSIAPTASPAASPESSISMA--------YTGTA 620
Query: 574 GAATIVLEPTDTSSLITSVALAPVEDSITSFTTTAGAATIVLEPTDTSSLITSV 735
P + + +S A E S T +T + ++I++ T++S S+
Sbjct: 621 -------SPESSIKVSSSTETASPESSFTEASTASPESSIMVASTESSGSFFSM 667
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,810,662
Number of Sequences: 28952
Number of extensions: 229426
Number of successful extensions: 711
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 479
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 691
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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