Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= maV30516
(552 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 123 7e-29
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 36 0.018
At2g44040.1 68415.m05475 dihydrodipicolinate reductase family pr... 32 0.22
At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase fam... 31 0.68
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 29 1.6
At3g59890.2 68416.m06683 dihydrodipicolinate reductase family pr... 29 1.6
At3g59890.1 68416.m06684 dihydrodipicolinate reductase family pr... 29 1.6
At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SD... 29 2.1
At2g34070.1 68415.m04171 expressed protein 29 2.7
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 3.6
At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger) fa... 28 3.6
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 3.6
At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase fam... 28 4.8
At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong... 27 8.3
At5g50310.1 68418.m06229 kelch repeat-containing protein similar... 27 8.3
At5g44400.1 68418.m05436 FAD-binding domain-containing protein s... 27 8.3
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 27 8.3
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 123 bits (297), Expect = 7e-29
Identities = 62/141 (43%), Positives = 96/141 (68%)
Frame = +3
Query: 126 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLG 305
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG TGF+GRY+ +L
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGATGFLGRYLVQQLA 89
Query: 306 KIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAXRYSNVVINLVGRDY 485
K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI +NVVINL+GR+Y
Sbjct: 90 KMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKAVMAKANVVINLIGREY 149
Query: 486 ETKNFKYNDVHVDGVRRIARI 548
ET+NF + D + ++A +
Sbjct: 150 ETRNFSFEDANHHIAEKLALV 170
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 35.9 bits (79), Expect = 0.018
Identities = 28/102 (27%), Positives = 49/102 (48%), Gaps = 4/102 (3%)
Frame = +3
Query: 255 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 422
V G TG +G+ + + K G R D AQ ++ DLG + L D+
Sbjct: 7 VIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDK 65
Query: 423 ESIAKAXRYSNVVINLVGRDYETKNFKYNDVHVDGVRRIARI 548
ES+ KA + +VVI+ VGR ++T+ ++ +D ++ +
Sbjct: 66 ESLVKAIKQVDVVISAVGR-FQTEILNQTNI-IDAIKESGNV 105
>At2g44040.1 68415.m05475 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae} ;
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 347
Score = 32.3 bits (70), Expect = 0.22
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYDAQRLKVCG 377
NGI V GC+G +G+ V G ++ + + D QR++VCG
Sbjct: 71 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCG 118
>At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 437
Score = 30.7 bits (66), Expect = 0.68
Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +3
Query: 228 SSFNGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 353
S+ NGI V G GFVG +V L + G +I L D+YD
Sbjct: 92 STNNGITVLVTGAAGFVGTHVSAALKRRGDGVIGLDNFNDYYD 134
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 29.5 bits (63), Expect = 1.6
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Frame = +3
Query: 135 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV---ATVFGCTGFVGRYVCNKLG 305
G+ S++++ + +Y+ R N + + GG FNG+V G G Y +
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLVDVYKKTLKSDGIAGLYRGFNIS 259
Query: 306 KIGTQLILPYRG---DFYDAQR-LKVCGDLGQVLFTPYHL 413
G I+ YRG YD+ + + + GDL F + L
Sbjct: 260 CAG---IIVYRGLYFGLYDSVKPVLLTGDLQDSFFASFAL 296
>At3g59890.2 68416.m06683 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 343
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 377
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 67 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 114
>At3g59890.1 68416.m06684 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 349
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 377
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 73 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 120
>At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SDR)
family protein similar to short-chain type
dehydrogenase/reductase SP:Q08632 [Picea abies]
Length = 270
Score = 29.1 bits (62), Expect = 2.1
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +3
Query: 240 GIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRL 365
G VA V G + +GR + L ++G ++++ Y DA+R+
Sbjct: 16 GRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 57
>At2g34070.1 68415.m04171 expressed protein
Length = 385
Score = 28.7 bits (61), Expect = 2.7
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 129 LNGS-MSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYV 290
L+G+ ++ Y+K + S K LAA GG+ G T GC F GR+V
Sbjct: 20 LSGADQALAYVKKPHVSQRNKTALAAVAGRGGGKEMLKGRKQT-SGCNLFQGRWV 73
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 3.6
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -1
Query: 246 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 160
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger)
family protein strong similarity to RING-H2 finger
protein RHA1a [Arabidopsis thaliana] GI:3790554;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 159
Score = 28.3 bits (60), Expect = 3.6
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = -1
Query: 411 DGKE*TGPGLNLRTLSTFGHHRNRLC-MVKLTGYQFFPI-CCTHSVRQIRCNRKLWLRCR 238
D E + P L LST N L +V+ + P CCT + + K+ +
Sbjct: 45 DHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPK 104
Query: 237 *SYCGHQYLAYMLQGWVYDPN 175
CGH + Y L W+ D N
Sbjct: 105 ---CGHVFHHYCLDRWIVDYN 122
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 28.3 bits (60), Expect = 3.6
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +3
Query: 270 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAXRY 449
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 450 SNVVIN 467
+++ N
Sbjct: 386 HDLIFN 391
>At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 434
Score = 27.9 bits (59), Expect = 4.8
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +3
Query: 237 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 353
NG+ V G GFVG +V L + G ++ L D+YD
Sbjct: 90 NGVSVLVTGAAGFVGTHVSAALKRRGDGVLGLDNFNDYYD 129
>At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong
similarity to pyruvate decarboxylase 1 [Vitis vinifera]
GI:10732644; contains InterPro entry IPR000399: Pyruvate
decarboxylase
Length = 607
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 252 TVFGCTGFVGRYVCNKLGKIGTQLILPYRGDF 347
TV C +GRY+ +L +IG + GDF
Sbjct: 39 TVSPCDATLGRYLARRLVEIGVTDVFSVPGDF 70
>At5g50310.1 68418.m06229 kelch repeat-containing protein similar to
Kelch repeats protein 3 (SP:Q08979) [Saccharomyces
cerevisiae]; contains Pfam PF01344: Kelch motif (6
repeats)
Length = 666
Score = 27.1 bits (57), Expect = 8.3
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +3
Query: 315 TQLILPYRGDFYDAQRLKVCGDL 383
T+LIL Y G+FY+ Q+ V GDL
Sbjct: 82 TELIL-YGGEFYNGQKTYVYGDL 103
>At5g44400.1 68418.m05436 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 537
Score = 27.1 bits (57), Expect = 8.3
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -1
Query: 318 GYQFFPICCTHSVRQIRCNRKLWLRCR*SYCGHQY--LAYMLQ 196
G+ F PI +H I C++KL + R GH Y L+Y+ Q
Sbjct: 85 GFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 127
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 27.1 bits (57), Expect = 8.3
Identities = 16/68 (23%), Positives = 29/68 (42%)
Frame = +3
Query: 135 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLGKIG 314
G+M++ K + N A + G G + S N + TGF G ++G G
Sbjct: 506 GTMTMKRKKPCDAGERTSANCANNETGNGKKVSVNNVGEAEPADTGFTGLTDNLRIGSFG 565
Query: 315 TQLILPYR 338
++++ R
Sbjct: 566 NEVVIELR 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,245,108
Number of Sequences: 28952
Number of extensions: 220664
Number of successful extensions: 528
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 528
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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