Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP02_F_H05
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga... 198 3e-51
At5g11490.1 68418.m01341 adaptin family protein similar to SP|Q9... 37 0.013
At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to SP... 35 0.041
At2g33330.1 68415.m04085 33 kDa secretory protein-related contai... 31 0.51
At1g04520.1 68414.m00443 33 kDa secretory protein-related contai... 29 2.0
At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing... 29 2.7
At2g20140.1 68415.m02353 26S protease regulatory complex subunit... 29 3.6
At4g28820.1 68417.m04120 zinc finger (HIT type) family protein c... 28 4.7
At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138... 28 4.7
At3g22990.1 68416.m02899 expressed protein 28 6.2
>At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative /
gamma-2 coat protein, putative / gamma-2 COP, putative
similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2
coat protein) (Gamma-2 COP) {Homo sapiens}; contains
Pfam profile: PF01602 Adaptin N terminal region
Length = 886
Score = 198 bits (482), Expect = 3e-51
Identities = 98/182 (53%), Positives = 123/182 (67%)
Frame = +1
Query: 100 RDVKDEDDNSSNPYQNLDKTIVLQETREFNQTLVIPRKCSLILTKILYLLNQGENFTTQE 279
+D +D+ +P+ ++K VLQE R FN V PR+CS ++TK+LYLLNQGE+FT E
Sbjct: 8 KDDDHDDELEYSPFMGIEKGAVLQEARVFNDPQVDPRRCSQVITKLLYLLNQGESFTKVE 67
Query: 280 ATDAFFATTKLFQSKEIMLRRMVYLCIKELSKLAQDVIIVTSSLTKDMTGKEDLYRAAAI 459
AT+ FF+ TKLFQSK+ LRRMVYL IKELS + +VIIVTSSL KDM K D+YRA AI
Sbjct: 68 ATEVFFSVTKLFQSKDTGLRRMVYLIIKELSPSSDEVIIVTSSLMKDMNSKIDMYRANAI 127
Query: 460 RALCSITDASMLQAIERYMKQAIVDKNPXXXXXXXXXXXXXXXXXPDLVRRWANEAXETI 639
R LC I D ++L IERY+KQAIVDKNP P++V+RW+NE E I
Sbjct: 128 RVLCRIIDGTLLTQIERYLKQAIVDKNPVVSSAALVSGLHLLKTNPEIVKRWSNEVQEGI 187
Query: 640 NS 645
S
Sbjct: 188 QS 189
>At5g11490.1 68418.m01341 adaptin family protein similar to
SP|Q9WV76 Adapter-related protein complex 4 beta 1
subunit (Beta subunit of AP- 4) {Mus musculus},
beta-adaptin Drosophila 1 {Drosophila melanogaster}
GI:434902; contains Pfam profile: PF01602 Adaptin N
terminal region
Length = 841
Score = 36.7 bits (81), Expect = 0.013
Identities = 18/75 (24%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +1
Query: 319 SKEIMLRRMVYLCIKELSKLAQDVIIVTSS-LTKDMTGKEDLYRAAAIRALCSITDASML 495
+ +I+L++M YL + +K D+ ++T + L +D ++ + R A+R+LCS+ +++
Sbjct: 74 TSDIVLKKMCYLYVGNYAKGNPDLSLLTINFLQRDCKDEDPMIRGLALRSLCSLRVPNLV 133
Query: 496 QAIERYMKQAIVDKN 540
+ + + + D N
Sbjct: 134 EYLVGPLGSGLKDNN 148
>At1g31730.1 68414.m03893 epsilon-adaptin, putative similar to
SP|Q9UPM8 Adapter-related protein complex 4 epsilon 1
subunit (Epsilon subunit of AP-4) (AP-4 adapter complex
epsilon subunit) {Homo sapiens}; contains Pfam profile:
PF01602 Adaptin N terminal region
Length = 938
Score = 35.1 bits (77), Expect = 0.041
Identities = 27/120 (22%), Positives = 54/120 (45%), Gaps = 5/120 (4%)
Frame = +1
Query: 160 IVLQETREFNQTLVIP----RKCSLILTKILYLLNQGENFTTQEATDAFFATTKLFQSKE 327
IVL E + L+ P RK + +++Y+ G +A+ + K+
Sbjct: 44 IVLSEVDILKRRLLEPDIPKRKMKEYIIRLVYIEMLGH-----DASFGYIYAVKMTHDDN 98
Query: 328 IMLRRMVYLCIKELSKLAQDVII-VTSSLTKDMTGKEDLYRAAAIRALCSITDASMLQAI 504
++L+R YL + D+II + +++ KD+ L AA+ A+C + + + A+
Sbjct: 99 LLLKRTGYLAVTLFLNEDHDLIILIVNTIQKDLRSDNYLVVCAALNAICRLINEETIPAV 158
>At2g33330.1 68415.m04085 33 kDa secretory protein-related contains
Pfam PF01657: Domain of unknown function, duplicated in
33 KDa secretory proteins
Length = 304
Score = 31.5 bits (68), Expect = 0.51
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +3
Query: 447 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 557
G VVQ HG YAT+Y ++Y G C + G C
Sbjct: 170 GVMQNGVVQGHG-FYATTYESVYVLGQCEGDIGDSDC 205
>At1g04520.1 68414.m00443 33 kDa secretory protein-related contains
Pfam PF01657: Domain of unknown function, duplicated in
33 KDa secretory proteins
Length = 307
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = +3
Query: 447 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 557
G VV HG YAT+Y ++Y G C + G C
Sbjct: 168 GVMQNGVVSGHG-FYATTYESVYVLGQCEGDVGDTDC 203
>At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing
protein similar to GCN4-complementing protein (GCP1)
GI:6465806 from [Arabidopsis thaliana]
Length = 850
Score = 29.1 bits (62), Expect = 2.7
Identities = 18/41 (43%), Positives = 24/41 (58%)
Frame = -1
Query: 515 IYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTM 393
+YR I CS EA V NAL A Y SLP+ +++ E T+
Sbjct: 686 VYRHIVCS-EADV---NALRGQASYTVSLPLSKMMQMEETL 722
>At2g20140.1 68415.m02353 26S protease regulatory complex subunit 4,
putative similar to Swiss-Prot:P48601 26S protease
regulatory subunit 4 (P26S4) [Drosophila melanogaster]
Length = 443
Score = 28.7 bits (61), Expect = 3.6
Identities = 24/74 (32%), Positives = 34/74 (45%)
Frame = +1
Query: 313 FQSKEIMLRRMVYLCIKELSKLAQDVIIVTSSLTKDMTGKEDLYRAAAIRALCSITDASM 492
F +I RR ++ LA+DV + +TKD D I+A+C T+A +
Sbjct: 356 FPLPDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGAD------IKAIC--TEAGL 407
Query: 493 LQAIERYMKQAIVD 534
L ER MK VD
Sbjct: 408 LALRERRMKVTHVD 421
>At4g28820.1 68417.m04120 zinc finger (HIT type) family protein
contains Pfam profile: PF04438 HIT zinc finger
Length = 173
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 121 DNSSNPYQNLDKTIVLQETREFNQTLV 201
D+SSNP Q LD+ + ++ REF ++
Sbjct: 137 DSSSNPLQELDEAMGIEAFREFTDKIL 163
>At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 562
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/59 (25%), Positives = 32/59 (54%)
Frame = -1
Query: 527 IACFIYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTMITSCANLLSSLMQR 351
I+C ++ A S +AS ++H + YRS V + ++ E+ + A+ +S++ +R
Sbjct: 77 ISCMLFHLHAASFDASPIIHRRPAPGSVYRSP-QVYARLRGEIEADNTTADAISTIWKR 134
>At3g22990.1 68416.m02899 expressed protein
Length = 460
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = -1
Query: 482 SVMLHNALIAAARYRSSLPVMSLVKEEVTMITSCANLLSSLMQR*TIRRSMISL 321
S+++HN+ + R L + S +K EVT + LL S ++ IRR ++ L
Sbjct: 56 SLLVHNSFVEQNNRRIVLALQSGLKSEVTWALNTLTLL-SFKEKEDIRRDVMPL 108
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,364,974
Number of Sequences: 28952
Number of extensions: 224043
Number of successful extensions: 540
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 539
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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