SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP02_F_F12
         (652 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putativ...   108   4e-24
At3g30560.1 68416.m03867 hypothetical protein                          33   0.16 
At5g05740.2 68418.m00632 peptidase M50 family protein / sterol-r...    32   0.38 
At1g20730.1 68414.m02597 expressed protein contains TIGRFAM TIGR...    30   1.5  
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA...    28   6.2  
At4g01910.1 68417.m00251 DC1 domain-containing protein contains ...    28   6.2  
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA...    28   6.2  
At1g50410.1 68414.m05650 SNF2 domain-containing protein / helica...    27   8.2  

>At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putative
           (UBP14) similar to ubiquitin-specific protease 14
           GI:11993473 [Arabidopsis thaliana]
          Length = 797

 Score =  108 bits (259), Expect = 4e-24
 Identities = 59/140 (42%), Positives = 90/140 (64%)
 Frame = +2

Query: 233 FGRNYVEQYFQKTGNAVFLHIRREKQPIPEPEQTGDGPEKKITKLAIGVEGGFDPECGKP 412
           FG++YV   ++KTGN V+LHI++ ++ IPE     D P KK T LAIGV+GGFD    +P
Sbjct: 48  FGKDYVSWNYEKTGNPVYLHIKQTRKSIPE-----DRPLKKPTLLAIGVDGGFDNN--EP 100

Query: 413 KFTYTDHYSVVVLPGFHTFPWPDPNLPEPVKKSVQAVIDAESPFKIAELAALQGTWDGEK 592
           +  Y + YS+V+LP F + P+P   LPE V+ +V  V++A    +  ++AA    W  EK
Sbjct: 101 E--YEESYSIVILPDFVSLPFPSVELPEKVRIAVDTVVNAVGAERKEQVAA----WTAEK 154

Query: 593 REVSVHSVNLKQLDNGIRIP 652
           + +S H++ L+Q+ +GI IP
Sbjct: 155 KLISEHALTLQQIKSGIVIP 174



 Score = 54.0 bits (124), Expect = 8e-08
 Identities = 23/46 (50%), Positives = 32/46 (69%)
 Frame = +1

Query: 91  LSLLTSHLSKIKLPGPHQLVYKDECVYSFDNPESETGLYVSLVSFL 228
           + LL S+LS++++P P   +YK EC  SFD P SE GL+V + SFL
Sbjct: 1   MELLRSNLSRVQIPEPTHRIYKHECCISFDTPRSEGGLFVDMNSFL 46


>At3g30560.1 68416.m03867 hypothetical protein
          Length = 1473

 Score = 33.1 bits (72), Expect = 0.16
 Identities = 19/49 (38%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
 Frame = +2

Query: 404 GKPKF-TYTDHYSVVVLPGFHTFPWPDPNLPEPVKKSVQAVIDAESPFK 547
           GK K  TYT  +    LP  H   W DP    P+   V  +I AE P K
Sbjct: 547 GKTKLATYTIEFQKRGLPHAHIIVWMDPRYKFPIADHVDKIIFAEIPDK 595


>At5g05740.2 68418.m00632 peptidase M50 family protein /
           sterol-regulatory element binding protein (SREBP) site 2
           protease family protein contains Pfam PF02163:
           Sterol-regulatory element binding protein (SREBP) site 2
           protease
          Length = 527

 Score = 31.9 bits (69), Expect = 0.38
 Identities = 22/62 (35%), Positives = 32/62 (51%), Gaps = 4/62 (6%)
 Frame = +2

Query: 479 DPNLPEPVKKSVQAVIDAESPFKIAELAALQGTWDGEKREVSVHS----VNLKQLDNGIR 646
           DP    P + + Q+ +  E+P    E  A   + DG+K EVS  S    VN  QLD+ +R
Sbjct: 90  DPPTKIPTELNSQSTVVNEAPGNEEENKAQFSSQDGDKLEVSSGSPLPGVNPLQLDDSMR 149

Query: 647 IP 652
           +P
Sbjct: 150 LP 151


>At1g20730.1 68414.m02597 expressed protein contains TIGRFAM
           TIGR01640 : F-box protein interaction domain
          Length = 567

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 24/74 (32%), Positives = 37/74 (50%)
 Frame = +1

Query: 70  TVIDMASLSLLTSHLSKIKLPGPHQLVYKDECVYSFDNPESETGLYVSLVSFLRIRTQLR 249
           T I  +S SLLT+ +  + L G    +  D  + +FD PE+E   +V  V F R     +
Sbjct: 370 TTITTSSKSLLTTWMKPVYLDGTLHWLRNDGSIIAFD-PETEQARFVPSV-FHRGGPGTK 427

Query: 250 *TVFPENRKRRLLT 291
             +F E+ K+  LT
Sbjct: 428 -LIFTEDSKKNRLT 440


>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
           GI:3021506
          Length = 374

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 11/41 (26%), Positives = 22/41 (53%)
 Frame = +2

Query: 521 VIDAESPFKIAELAALQGTWDGEKREVSVHSVNLKQLDNGI 643
           +I  ++  ++AE A L     G +R  ++H  N+ Q  +G+
Sbjct: 182 IITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGL 222


>At4g01910.1 68417.m00251 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 651

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 1/65 (1%)
 Frame = +2

Query: 218 CHSCGFGRNYVEQYFQKTGNAVFLHIRREKQPIPEPEQTGDGPEKKITKLAIGVEG-GFD 394
           C  CG   NY+  Y +  G  V LH      P PE  +  +    K+T L   +     D
Sbjct: 80  CDLCGRRTNYLSYYCEICGFIVDLHC--AMYPPPEFIENSERHHHKLTLLKERIAAFDCD 137

Query: 395 PECGK 409
            +CGK
Sbjct: 138 AKCGK 142


>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
           [Nicotiana tabacum]
          Length = 374

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 11/41 (26%), Positives = 22/41 (53%)
 Frame = +2

Query: 521 VIDAESPFKIAELAALQGTWDGEKREVSVHSVNLKQLDNGI 643
           +I  ++  ++AE A L     G K+  ++H  N+ Q  +G+
Sbjct: 182 IITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDGL 222


>At1g50410.1 68414.m05650 SNF2 domain-containing protein / helicase
           domain-containing protein / RING finger
           domain-containing protein similar to transcription
           factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930;
           contains Pfam profiles PF00271: Helicase conserved
           C-terminal domain, PF00176: SNF2 family N-terminal
           domain, PF00097: Zinc finger, C3HC4 type (RING finger)
          Length = 981

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 13/51 (25%), Positives = 26/51 (50%)
 Frame = +1

Query: 64  YSTVIDMASLSLLTSHLSKIKLPGPHQLVYKDECVYSFDNPESETGLYVSL 216
           ++ ++D+  LSL+ + +   +L G   L+ +D  V  F N      + +SL
Sbjct: 835 WTGMLDLVELSLIENSIEFRRLDGTMSLIARDRAVKEFSNDPDVKVMIMSL 885


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,247,660
Number of Sequences: 28952
Number of extensions: 356021
Number of successful extensions: 1061
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1030
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1059
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -