Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP02_F_E05
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing ...    55   4e-08
At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing ...    47   1e-05
At2g28530.1 68415.m03466 RNA recognition motif (RRM)-containing ...    46   2e-05
At1g74870.1 68414.m08677 expressed protein contains similarity t...    38   0.004
At3g48070.1 68416.m05241 expressed protein                             34   0.095
At5g62910.1 68418.m07894 expressed protein predicted proteins, A...    33   0.17 
At2g20300.1 68415.m02371 protein kinase family protein contains ...    31   0.51 
At4g30790.1 68417.m04362 expressed protein                             30   1.2  

>At3g45630.1 68416.m04928 RNA recognition motif (RRM)-containing
           protein similar to SP|P34909 General negative regulator
           of transcription subunit 4 {Saccharomyces cerevisiae};
           contains InterPro entry IPR000504: RNA-binding region
           RNP-1 (RNA recognition motif) (RRM)
          Length = 989

 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 26/73 (35%), Positives = 45/73 (61%)
 Frame = +3

Query: 435 ACRKAYPENPADFIPLSQEQVAAIXTDXKAREXKRRXKTLESRRALANVRVVQNNLXFVV 614
           ACR  Y +     + + QE++A+     + +  K + K+ + R+ L +VRVVQ NL ++V
Sbjct: 55  ACRTPYDKEKIVGMTVDQERLASEGNMDRKKIQKSKPKSSDGRKPLTSVRVVQRNLVYIV 114

Query: 615 GLPVRLADPEILK 653
           GLP+ LAD ++L+
Sbjct: 115 GLPLNLADEDLLQ 127



 Score = 32.3 bits (70), Expect = 0.29
 Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 5/35 (14%)
 Frame = +2

Query: 344 DLHFYPCTCGYQICRFRWNRI-----REGXNGLCP 433
           D    PC CGYQIC + W+ I     ++   G CP
Sbjct: 20  DQQLKPCKCGYQICVWCWHHIMDMAEKDQSEGRCP 54


>At5g60170.1 68418.m07543 RNA recognition motif (RRM)-containing
           protein low similarity to transcriptional repressor
           Not4-Np [Homo sapiens] GI:6856207; contains
           INTERPRO:IPR000504 RNA-binding region RNP-1 (RNA
           recognition motif)
          Length = 944

 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 24/74 (32%), Positives = 44/74 (59%), Gaps = 1/74 (1%)
 Frame = +3

Query: 435 ACRKAYPENPADFIPLSQEQVAAIXTDXKAREXKRRXKTLESRRA-LANVRVVQNNLXFV 611
           ACR  Y +     + ++ + +A+     + +  K + K+ E R+  L +VRV+Q NL ++
Sbjct: 13  ACRTPYDKEKIVGMTVNCDSLASEGNMERKKIQKSKSKSSEGRKQQLTSVRVIQRNLVYI 72

Query: 612 VGLPVRLADPEILK 653
           VGLP+ LAD ++L+
Sbjct: 73  VGLPLNLADEDLLQ 86


>At2g28530.1 68415.m03466 RNA recognition motif (RRM)-containing
           protein similar to SP|P34909 General negative regulator
           of transcription subunit 4 {Saccharomyces cerevisiae};
           contains InterPro entry IPR000504: RNA-binding region
           RNP-1 (RNA recognition motif) (RRM)
          Length = 236

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 24/73 (32%), Positives = 39/73 (53%)
 Frame = +3

Query: 435 ACRKAYPENPADFIPLSQEQVAAIXTDXKAREXKRRXKTLESRRALANVRVVQNNLXFVV 614
           ACR  Y +     + +S E++ A     + +  K + K  E R+ L  VRV+Q NL +V+
Sbjct: 55  ACRTRYDKEKIVGMTVSCERLVAEFYIDRKKSQKAKPKPAEGRKDLTGVRVIQRNLVYVM 114

Query: 615 GLPVRLADPEILK 653
            LP  LAD ++ +
Sbjct: 115 SLPFDLADEDMFQ 127



 Score = 35.5 bits (78), Expect = 0.031
 Identities = 13/23 (56%), Positives = 15/23 (65%)
 Frame = +2

Query: 344 DLHFYPCTCGYQICRFRWNRIRE 412
           D H  PC CGYQIC + W+ I E
Sbjct: 20  DQHLKPCKCGYQICVWCWHHIIE 42


>At1g74870.1 68414.m08677 expressed protein contains similarity to
           hypothetical proteins
          Length = 289

 Score = 38.3 bits (85), Expect = 0.004
 Identities = 16/30 (53%), Positives = 19/30 (63%)
 Frame = +2

Query: 344 DLHFYPCTCGYQICRFRWNRIREGXNGLCP 433
           DL F PCTCG++IC F  N+I E     CP
Sbjct: 223 DLEFEPCTCGFRICLFCHNKISEN-EARCP 251


>At3g48070.1 68416.m05241 expressed protein
          Length = 319

 Score = 33.9 bits (74), Expect = 0.095
 Identities = 16/51 (31%), Positives = 25/51 (49%)
 Frame = +2

Query: 344 DLHFYPCTCGYQICRFRWNRIREGXNGLCPRLSEGIPRKPGRLHSSQSRAG 496
           D +F PC CG+++C F    I +G +G CP   +   R   +  +S    G
Sbjct: 255 DSNFLPCPCGFRLCLFCHKTICDG-DGRCPGCRKPYERNTIKFEASVQGGG 304


>At5g62910.1 68418.m07894 expressed protein predicted proteins,
           Arabidopsis thaliana
          Length = 327

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 344 DLHFYPCTCGYQICRFRWNRIREGXNGLCP 433
           D +F PC CG+++C F    I +G +G CP
Sbjct: 263 DSNFLPCPCGFRLCLFCHKTICDG-DGRCP 291


>At2g20300.1 68415.m02371 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 744

 Score = 31.5 bits (68), Expect = 0.51
 Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 2/40 (5%)
 Frame = +2

Query: 392 RWNRIREGXNGLCPRLSEGIPRKP--GRLHSSQSRAGGSD 505
           +W +I +  N + P L+  I ++P  G + SS +R+ GSD
Sbjct: 282 KWKKIGKSSNAVGPALAPSINKRPGAGSMFSSSARSSGSD 321


>At4g30790.1 68417.m04362 expressed protein
          Length = 1148

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 10/27 (37%), Positives = 19/27 (70%)
 Frame = -2

Query: 412  FSNTIPAKPTYLVSTCTRVEVEVVHLP 332
            F++ +P++PTY+V     +E ++V LP
Sbjct: 1052 FTDHLPSRPTYIVGQIVHIERQIVKLP 1078


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,683,775
Number of Sequences: 28952
Number of extensions: 238613
Number of successful extensions: 718
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)