Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10g17f
(601 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 120 1e-27
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 118 3e-27
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 113 6e-26
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 112 2e-25
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 112 2e-25
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 111 4e-25
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 29 1.8
At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to e... 29 3.1
At2g41590.1 68415.m05139 expressed protein similar to zinc finge... 29 3.1
At4g11540.1 68417.m01851 DC1 domain-containing protein contains ... 28 4.1
At5g49270.1 68418.m06098 phytochelatin synthetase-related contai... 28 5.4
At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 5.4
At5g19280.1 68418.m02298 kinase associated protein phosphatase (... 27 7.2
At2g13640.1 68415.m01503 Golgi GDP mannose transporter (GONST1) ... 27 7.2
At1g53180.1 68414.m06027 expressed protein 27 7.2
At1g32880.1 68414.m04051 importin alpha-1 subunit, putative simi... 27 7.2
At1g27080.1 68414.m03301 proton-dependent oligopeptide transport... 27 7.2
At3g63500.2 68416.m07153 expressed protein 27 9.5
At3g63500.1 68416.m07152 expressed protein 27 9.5
At1g18550.1 68414.m02314 kinesin motor protein-related contains ... 27 9.5
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 120 bits (288), Expect = 1e-27
Identities = 57/109 (52%), Positives = 73/109 (66%), Gaps = 2/109 (1%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 458
KI+A YIWI GSG +R K RTL P LP WN+DGSST+QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAI 77
Query: 459 YKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD--KCKDDEPWF 599
+KDPFR+GN+ILVMCD Y+ +P +NNR + +D K +EPWF
Sbjct: 78 FKDPFRKGNNILVMCDAYRPAGDPIPTNNRHKAVKIFDHPNVKAEEPWF 126
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 118 bits (284), Expect = 3e-27
Identities = 62/148 (41%), Positives = 86/148 (58%), Gaps = 3/148 (2%)
Frame = +3
Query: 165 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 341
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 342 TLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYN 521
T+ + P +LP WN+DGSST QA G +S+ L P+AI++DPFR GN+ILV+CDT+
Sbjct: 97 TIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRGGNNILVICDTWTPA 156
Query: 522 MEPTESNNRISCQEAYD--KCKDDEPWF 599
EP +N R E + K + PWF
Sbjct: 157 GEPIPTNKRAKAAEIFSNKKVSGEVPWF 184
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 113 bits (273), Expect = 6e-26
Identities = 54/110 (49%), Positives = 69/110 (62%), Gaps = 2/110 (1%)
Frame = +3
Query: 276 DKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRA 455
DKI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L P+A
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQA 76
Query: 456 IYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDK--CKDDEPWF 599
I+KDPFRRGN+ILVMCD Y EP +N R + + + + PW+
Sbjct: 77 IFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSNPDVAAEVPWY 126
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 112 bits (269), Expect = 2e-25
Identities = 55/116 (47%), Positives = 72/116 (62%), Gaps = 2/116 (1%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
DL D+I+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 438 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD--KCKDDEPWF 599
+ P+AI+KDPFRRGN+ILVMCD Y EP +N R + + ++ +E W+
Sbjct: 71 IIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFEDPSVVAEETWY 126
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 112 bits (269), Expect = 2e-25
Identities = 53/104 (50%), Positives = 68/104 (65%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
D+ ++KI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 438 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAY 569
L P+AI+KDPFRRGN+ILVMCD Y EP +N R + E +
Sbjct: 71 ILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIF 114
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 111 bits (266), Expect = 4e-25
Identities = 53/109 (48%), Positives = 70/109 (64%), Gaps = 2/109 (1%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 458
KI+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAI 77
Query: 459 YKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQE--AYDKCKDDEPWF 599
+KDPFR+GN+ILVMCD Y +P +N R + + ++ +EPW+
Sbjct: 78 FKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNAAKIFSHPDVAKEEPWY 126
>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 638
Score = 29.5 bits (63), Expect = 1.8
Identities = 17/80 (21%), Positives = 39/80 (48%)
Frame = -1
Query: 253 YRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYVLKCSLDASVEYCAK 74
+ + +L+ + + +KT PD+ ++ L C V +L++ R + L S + +K
Sbjct: 16 WSMEDILNKDLYKQKIKTIPDKFSSVNEYLKCFVPHLLEETRTE------LSTSFKSLSK 69
Query: 73 TYLRKVLSERKMFRSPHGAV 14
+L ++ S M + G++
Sbjct: 70 AHLFEISSLETMTKESSGSL 89
>At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to
expansin GI:11191999 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 252
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = -3
Query: 479 STEWILVNSTRYEVGIRIVPISLVG*AAIEVPYRQIFGSFRYEIQGAIF 333
S WI ++T Y G+ P SL G + PY FG+ + G +F
Sbjct: 24 SNGWIRAHATYY--GVNDSPASLGGACGYDNPYHAGFGAHTAALSGELF 70
>At2g41590.1 68415.m05139 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 367
Score = 28.7 bits (61), Expect = 3.1
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 249 RYNDLPLPADKILATYIWID-GSGEHLRCKDRTLNF 353
R + PL D+ +A Y +D G +H+RC+ + NF
Sbjct: 263 RISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNF 298
>At4g11540.1 68417.m01851 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 525
Score = 28.3 bits (60), Expect = 4.1
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = -2
Query: 432 QNCAHQLGWLSCHRSSI*ADLWEL*V*NSRCDLCTSGVRQSRRSKCRWRESCQPAVEDHC 253
Q+ +H+ +L H + + LWE N RC CT VR C+ C + HC
Sbjct: 286 QHFSHKEHYLRFHVNGL---LWEE---NKRCSACTHPVRLQSFYACK---DCDFILHQHC 336
Query: 252 IDS 244
+S
Sbjct: 337 AES 339
>At5g49270.1 68418.m06098 phytochelatin synthetase-related contains
Pfam PF04833: Phytochelatin synthetase-like conserved
region
Length = 663
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -3
Query: 518 VFIRITHNEDVISSTEWILVNST 450
VF+ HNE +IS+T+ ++VN T
Sbjct: 91 VFVGFQHNEILISATDALIVNGT 113
>At4g32880.1 68417.m04679 homeobox-leucine zipper transcription
factor (HB-8) identical to HD-zip transcription factor
(athb-8) (GI:7270235) [Arabidopsis thaliana]
Length = 833
Score = 27.9 bits (59), Expect = 5.4
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +2
Query: 296 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 397
H D W PEV RS E T +QR+ LR+
Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365
>At5g19280.1 68418.m02298 kinase associated protein phosphatase
(KAPP) identical to Kinase associated protein
phosphatase (SP:P46014) [Arabidopsis thaliana]; contains
Pfam PF00481: Protein phosphatase 2C domain; contains
Pfam PF00498: FHA domain
Length = 581
Score = 27.5 bits (58), Expect = 7.2
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Frame = +3
Query: 261 LPLPADK----ILATYIWIDGSGEHLRC---KDRTLNFIPKAPKDLPIWNF 392
L LPA+K + TY W + E+L+ KDR +N P+ +D W F
Sbjct: 133 LKLPAEKGSLAEVQTYDWQNNRNENLQYNLEKDRLINLSPRLVEDQRSWLF 183
>At2g13640.1 68415.m01503 Golgi GDP mannose transporter (GONST1)
identical to cDNA GDP-Mannose transporter (GONST1)
GI:15487236; PMID 11595802
Length = 384
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +3
Query: 423 HNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDDE 590
H SD A+ KD + H+L MC K EP +++ K K+DE
Sbjct: 98 HGSDNSFTA-ALTKDRLKMKKHVLKMCSELKKKEEPGTMARQVTAFSVL-KNKEDE 151
>At1g53180.1 68414.m06027 expressed protein
Length = 358
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 319 SPEPSIQM*VARILSAGSGRSLYRLSSVLDSTAFGELVK 203
SPE + ++L A +GRSL R SS+ DS G K
Sbjct: 49 SPEKMGDSDIGKVLEASTGRSLIRASSI-DSREGGSQTK 86
>At1g32880.1 68414.m04051 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 183
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = -1
Query: 292 VARILSAGSGRSLYRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYV 116
V R++ S +L SV+D+ LVK + F + VCA+S HD +
Sbjct: 26 VHRLIQFLLDESFPKLQSVIDANLIPTLVKLTQNAEFDMKKESVCAISNATLLGSHDQI 84
>At1g27080.1 68414.m03301 proton-dependent oligopeptide transport
(POT) family protein similar to nitrate transporter
NRT1-5 [Glycine max] GI:11933414; contains Pfam profile
PF00854: POT family
Length = 525
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +1
Query: 463 RIHSVEEITSSLCVIRINTTWSQQRATIASVVKKLTTNARMM 588
R+ S++E+ C+IR+ WS A I S+V +TT A M
Sbjct: 259 RLCSIQEVEEVKCLIRVVPVWS---AGIISIV-AMTTQATFM 296
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -2
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 911 LNFAREWKFERFCKELEYVNKIFSSSKD 938
>At3g63500.1 68416.m07152 expressed protein
Length = 887
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -2
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 636 LNFAREWKFERFCKELEYVNKIFSSSKD 663
>At1g18550.1 68414.m02314 kinesin motor protein-related contains
similarity to kinesin-related protein GI:4493964 from
[Plasmodium falciparum]
Length = 799
Score = 27.1 bits (57), Expect = 9.5
Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = -1
Query: 349 FKVRSLHLRCSPEPSIQM*VARIL-SAGSGRSLYRLSSVLDSTAFGELVKTG----PDRI 185
F +R+ + SP+P Q ++L S+ G +RL DST E K DRI
Sbjct: 52 FSLRNPNHNQSPKPEDQRFKTQLLDSSKKGDRFHRLDGK-DSTFVEEDTKENNVFEADRI 110
Query: 184 FGLSSILVCAVSAILK 137
FG+SS+ V I K
Sbjct: 111 FGVSSVPVKPSGVIRK 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,836,527
Number of Sequences: 28952
Number of extensions: 307169
Number of successful extensions: 814
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 814
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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