Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_O10
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 336 1e-92
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 336 1e-92
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 336 1e-92
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 336 1e-92
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 187 5e-48
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 186 9e-48
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 100 1e-21
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 97 1e-20
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 3e-07
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 4e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 4e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 46 3e-05
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 4e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 4e-04
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 39 0.003
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 39 0.003
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.004
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.006
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.013
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.013
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.13
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 31 0.51
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.2
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.5
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.5
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 336 bits (825), Expect = 1e-92
Identities = 158/196 (80%), Positives = 173/196 (88%)
Frame = +3
Query: 66 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVL 245
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR I +FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 246 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 425
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 426 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKK 605
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ ++EI EVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 606 IGYNPAAVAFVPISGW 653
+GYNP + FVPISG+
Sbjct: 181 VGYNPDKIPFVPISGF 196
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 336 bits (825), Expect = 1e-92
Identities = 158/196 (80%), Positives = 173/196 (88%)
Frame = +3
Query: 66 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVL 245
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR I +FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 246 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 425
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 426 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKK 605
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ ++EI EVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 606 IGYNPAAVAFVPISGW 653
+GYNP + FVPISG+
Sbjct: 181 VGYNPDKIPFVPISGF 196
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 336 bits (825), Expect = 1e-92
Identities = 158/196 (80%), Positives = 173/196 (88%)
Frame = +3
Query: 66 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVL 245
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR I +FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 246 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 425
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 426 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKK 605
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ ++EI EVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 606 IGYNPAAVAFVPISGW 653
+GYNP + FVPISG+
Sbjct: 181 VGYNPDKIPFVPISGF 196
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 336 bits (825), Expect = 1e-92
Identities = 158/196 (80%), Positives = 173/196 (88%)
Frame = +3
Query: 66 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVL 245
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR I +FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 246 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 425
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 426 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKK 605
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ ++EI EVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 606 IGYNPAAVAFVPISGW 653
+GYNP + FVPISG+
Sbjct: 181 VGYNPDKIPFVPISGF 196
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 187 bits (456), Expect = 5e-48
Identities = 89/193 (46%), Positives = 129/193 (66%), Gaps = 1/193 (0%)
Frame = +3
Query: 75 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKL 254
+K H+N+V IGHVD+GKST G +++ G +D R I K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 255 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 434
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 435 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKKIGY 614
E G + GQTREH LA TLGV +LIV VNKMD +S+ ++EI ++ ++K GY
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGY 277
Query: 615 NPAA-VAFVPISG 650
N V F+PISG
Sbjct: 278 NTKKDVVFLPISG 290
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 186 bits (454), Expect = 9e-48
Identities = 84/190 (44%), Positives = 130/190 (68%), Gaps = 1/190 (0%)
Frame = +3
Query: 81 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKA 260
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 261 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 440
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 441 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKKIGYN 617
G GQTREHA + GV+Q+IV +NKMD YS+ F+ I V S+++ +
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFK 415
Query: 618 PAAVAFVPIS 647
+++ ++P+S
Sbjct: 416 DSSLTWIPLS 425
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 100 bits (239), Expect = 1e-21
Identities = 63/179 (35%), Positives = 90/179 (50%), Gaps = 1/179 (0%)
Frame = +3
Query: 63 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWV 242
K ++K H+NI IGHVD GK+T T L I K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 243 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 422
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 423 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFE-EIXXEVSSY 596
G QT+EH LLA +GV ++V +NK D + E E+ +SSY
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 96.7 bits (230), Expect = 1e-20
Identities = 62/177 (35%), Positives = 89/177 (50%)
Frame = +3
Query: 72 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDK 251
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 252 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 431
E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 432 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYIK 602
QT+EH LLA +GV L+ +NK+D + P E E+ S+ K
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYK 217
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.0 bits (129), Expect = 2e-08
Identities = 40/128 (31%), Positives = 59/128 (46%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
NI + H+DSGK+T T +++ G I E E+ +G +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH---------EIHEV-RGRDGVGAKMDSMDLERE 116
Query: 270 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 449
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 450 KNGQTREH 473
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.0 bits (129), Expect = 2e-08
Identities = 40/128 (31%), Positives = 59/128 (46%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
NI + H+DSGK+T T +++ G I E E+ +G +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH---------EIHEV-RGRDGVGAKMDSMDLERE 116
Query: 270 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 449
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 450 KNGQTREH 473
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.0 bits (119), Expect = 3e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 270 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 428
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 51.6 bits (118), Expect = 4e-07
Identities = 52/174 (29%), Positives = 80/174 (45%), Gaps = 4/174 (2%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 270 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 437
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190
Query: 438 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXFEEIXXEVSSYI 599
A QT + LA + ++I +NK+D P +EP E++ E+ I
Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.6 bits (118), Expect = 4e-07
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +3
Query: 78 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLK 257
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 258 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 428
E+ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 45.6 bits (103), Expect = 3e-05
Identities = 53/169 (31%), Positives = 70/169 (41%), Gaps = 9/169 (5%)
Frame = +3
Query: 54 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKY 233
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 234 AWVLDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTS 386
LDKL +RERGIT+ E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLS 157
Query: 387 QADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 533
A+L+V A G QT + LAF + ++ +NK+D
Sbjct: 158 ACQGALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKID 198
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.9 bits (94), Expect = 4e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 270 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 449
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 450 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXF 566
QTR A G ++V VNK+D P + P F
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.9 bits (94), Expect = 4e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 270 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 449
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 450 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPXF 566
QTR A G ++V VNK+D P + P F
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 39.1 bits (87), Expect = 0.003
Identities = 45/172 (26%), Positives = 76/172 (44%), Gaps = 14/172 (8%)
Frame = +3
Query: 60 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIXKFEKEAQ-EMGKGSFK- 230
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 231 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 377
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 378 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 533
G + D A+L++AA QT EH + +K +I+ NK+D
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKID 192
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 39.1 bits (87), Expect = 0.003
Identities = 45/172 (26%), Positives = 76/172 (44%), Gaps = 14/172 (8%)
Frame = +3
Query: 60 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIXKFEKEAQ-EMGKGSFK- 230
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 231 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 377
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 378 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 533
G + D A+L++AA QT EH + +K +I+ NK+D
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKID 192
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 38.7 bits (86), Expect = 0.003
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IXKFEKEAQEMGKG-SFKYAWVLDKLK 257
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 258 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 413
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 38.3 bits (85), Expect = 0.004
Identities = 43/166 (25%), Positives = 72/166 (43%), Gaps = 8/166 (4%)
Frame = +3
Query: 60 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKRTIXKFEKEAQEMGKGS 224
P++ + INI IGHV GKST +G H + +++ K ++ K
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 225 FKYAWVLDK-LKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 395
V K + +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 396 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 533
A+LI+AA QT EH + +K +I+ NK+D
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKID 184
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 37.9 bits (84), Expect = 0.006
Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 4/81 (4%)
Frame = +3
Query: 303 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 470
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 471 HALLAFTLGVKQLIVGVNKMD 533
H + +K +I+ NK+D
Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.013
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 270 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 428
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.013
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 90 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAQEMGKGSFKYAWVLDKLKAERE 269
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 270 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 428
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 33.5 bits (73), Expect = 0.13
Identities = 32/105 (30%), Positives = 49/105 (46%), Gaps = 3/105 (2%)
Frame = +3
Query: 324 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 503
+T +D PGH F + G + D VL+VAA G QT E A + V
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321
Query: 504 QLIVGVNKMDSTEPPYSEPXFEEIXXEVSS---YIKKIGYNPAAV 629
++V +NK D P + P E++ +++S ++ IG N AV
Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTSEGIELEDIGGNVQAV 361
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 31.5 bits (68), Expect = 0.51
Identities = 27/96 (28%), Positives = 41/96 (42%), Gaps = 1/96 (1%)
Frame = +3
Query: 333 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 509
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 510 IVGVNKMDSTEPPYSEPXFEEIXXEVSSYIKKIGYN 617
+ +DS E E+ E S+ K++G N
Sbjct: 286 NLSTFTLDSDE--------EDEVREESNVAKEVGLN 313
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 1.2
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +3
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 509
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 510 IVGVNKMD 533
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 30.3 bits (65), Expect = 1.2
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +3
Query: 324 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 503
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 504 QLIVGVNKMD 533
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIV 413
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIV 413
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.5 bits (63), Expect = 2.0
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +3
Query: 249 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 416
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 417 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 533
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,882,629
Number of Sequences: 28952
Number of extensions: 273571
Number of successful extensions: 772
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 731
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 757
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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