Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_N06
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31740.1 68415.m03876 expressed protein 140 1e-33
At3g60910.1 68416.m06814 expressed protein low similarity to PIR... 94 6e-20
At3g17365.1 68416.m02219 expressed protein low similarity to PIR... 93 2e-19
At4g34360.1 68417.m04882 protease-related similar to PIR|I46078 ... 88 4e-18
At1g66680.1 68414.m07579 S locus-linked protein, putative simila... 44 9e-05
At1g73600.2 68414.m08520 phosphoethanolamine N-methyltransferase... 36 0.023
At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase... 36 0.023
At2g45300.1 68415.m05638 3-phosphoshikimate 1-carboxyvinyltransf... 32 0.29
At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family prot... 31 0.88
At1g48860.2 68414.m05470 3-phosphoshikimate 1-carboxyvinyltransf... 30 1.2
At1g48860.1 68414.m05471 3-phosphoshikimate 1-carboxyvinyltransf... 30 1.2
At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase... 30 1.5
At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase... 29 2.7
At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase... 29 2.7
At4g20470.1 68417.m02986 hypothetical protein 29 3.5
At5g43890.1 68418.m05367 flavin-containing monooxygenase family ... 28 6.2
At4g19440.1 68417.m02860 pentatricopeptide (PPR) repeat-containi... 28 6.2
At3g48290.1 68416.m05270 cytochrome P450, putative very strong s... 28 6.2
>At2g31740.1 68415.m03876 expressed protein
Length = 760
Score = 140 bits (338), Expect = 1e-33
Identities = 76/174 (43%), Positives = 106/174 (60%), Gaps = 9/174 (5%)
Frame = +3
Query: 129 KEFSEKDYWNKFFXKRGNK-AFEWYGEYLELCGQLNKYIKTKDS-------ILITGCGNS 284
++F+ K+ W+KFF RGN +FEWY E+ +L L ++ S IL+ GCGNS
Sbjct: 21 EDFTSKENWDKFFTLRGNDDSFEWYAEWPQLHDSLLPLLQDSSSSSSDSLQILVPGCGNS 80
Query: 285 SLSADLYDVGYVNITNVDVSEVVIKQMKNVN-KARTNMKFMCMDATRMSFEXEAFNVVLX 461
L+ LYD G+ +ITNVD S+VVI M N + R +++ MD T+M E+F+ VL
Sbjct: 81 RLTEHLYDAGFRDITNVDFSKVVISDMLRRNIRTRPELRWRVMDITKMQLADESFDTVLD 140
Query: 462 KGTLDALMPDATEXTNAIIXKYFSEIKRVLKLGGRFVCISLLQSHILAKLMEGF 623
KG LDALM E + +Y SE KRVLK GG+F+C++L +SH+LA L F
Sbjct: 141 KGALDALM--EPEVGTKLGNQYLSEAKRVLKPGGKFICLTLAESHVLALLFSRF 192
>At3g60910.1 68416.m06814 expressed protein low similarity to
PIR|I46078 endothelin converting enzyme from Bos
primigenius taurus
Length = 252
Score = 94.3 bits (224), Expect = 6e-20
Identities = 49/145 (33%), Positives = 82/145 (56%), Gaps = 1/145 (0%)
Frame = +3
Query: 150 YWNKFFXKRGNKAFEWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNIT 329
YW+ + + +F+WY Y L + ++ T +L+ GCGNS +S D+ GY +I
Sbjct: 18 YWDARYVQDA-LSFDWYQCYSSLRPFVRSFVSTSSRVLMVGCGNSLMSEDMVKDGYEDIM 76
Query: 330 NVDVSEVVIKQMKNVNKARTNMKFMCMDATRMS-FEXEAFNVVLXKGTLDALMPDATEXT 506
NVD+S V I+ M+ + +K+M MD MS FE ++F+ ++ KGTLD+LM +
Sbjct: 77 NVDISSVAIEMMQTKYASVPQLKYMQMDVRDMSYFEDDSFDTIIDKGTLDSLMCGSDALL 136
Query: 507 NAIIXKYFSEIKRVLKLGGRFVCIS 581
+A + E+ R++K GG + I+
Sbjct: 137 SA--SRMLGEVSRLIKPGGTYFLIT 159
>At3g17365.1 68416.m02219 expressed protein low similarity to
PIR|I46078 endothelin converting enzyme from Bos
primigenius taurus
Length = 239
Score = 92.7 bits (220), Expect = 2e-19
Identities = 49/153 (32%), Positives = 87/153 (56%), Gaps = 2/153 (1%)
Frame = +3
Query: 129 KEFSEKDYWNKFFXKRGNKAFEWYGEYLELCGQLNKYIKTKDS-ILITGCGNSSLSADLY 305
+ +SE+ YW+ + K ++ F+WY +Y L +N Y+ ++ +L+ GCGNS+ S +
Sbjct: 8 QSYSEQWYWDDRY-KNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAFSEGMV 66
Query: 306 DVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDATRM-SFEXEAFNVVLXKGTLDAL 482
D GY ++ ++D+S VVI M R +K++ MD M +FE +F+ V+ KGTLD++
Sbjct: 67 DDGYEDVVSIDISSVVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKGTLDSI 126
Query: 483 MPDATEXTNAIIXKYFSEIKRVLKLGGRFVCIS 581
+ + + E+ RVLK G ++ I+
Sbjct: 127 L--CGSNSRQYSTQMLEEVWRVLKDKGVYILIT 157
>At4g34360.1 68417.m04882 protease-related similar to PIR|I46078
endothelin converting enzyme, Bos primigenius taurus
Length = 248
Score = 88.2 bits (209), Expect = 4e-18
Identities = 54/175 (30%), Positives = 89/175 (50%), Gaps = 8/175 (4%)
Frame = +3
Query: 117 PRSHKEFSEKDYWNKFFXKRGNKAFEWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSA 296
P S + + YW++ F + +EW+ +Y + IK S+L GCGNS L
Sbjct: 9 PPSALTYLDPHYWDERFSSEEH--YEWFKDYSHFQHLIISNIKPSSSVLELGCGNSQLCE 66
Query: 297 DLYDVGYVNITNVDVSEVVIKQMKN--VNKARTNMKFMCMDATRMSFEXEAFNVVLXKGT 470
+LY G V+IT +D+S V +++M++ + K +K + D + F+ E+F+VV+ KGT
Sbjct: 67 ELYKDGIVDITCIDLSSVAVEKMQSRLLPKGYKEIKVVQADMLDLPFDSESFDVVIEKGT 126
Query: 471 LDALMPDATEXTN------AIIXKYFSEIKRVLKLGGRFVCISLLQSHILAKLME 617
+D L DA + N + + + RVLK G F+ I+ Q H L +
Sbjct: 127 MDVLFVDAGDPWNPRPETVSKVMATLDGVHRVLKPDGIFISITFGQPHFRRPLFK 181
>At1g66680.1 68414.m07579 S locus-linked protein, putative similar
to S locus-linked protein SLL2 [Brassica napus]
GI:1518113
Length = 358
Score = 44.0 bits (99), Expect = 9e-05
Identities = 35/137 (25%), Positives = 68/137 (49%), Gaps = 2/137 (1%)
Frame = +3
Query: 213 ELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKAR-- 386
E+ Q +KY+ + + +L G GN L L G+ ++T D S+ ++ +++++
Sbjct: 159 EVNDQADKYLSSWN-VLDLGTGNGLLLHQLAKEGFSDLTGTDYSDGAVELAQHLSQRDGF 217
Query: 387 TNMKFMCMDATRMSFEXEAFNVVLXKGTLDALMPDATEXTNAIIXKYFSEIKRVLKLGGR 566
N++FM D E + F +V+ KGTLDA+ ++ Y+ + +++ GG
Sbjct: 218 PNIRFMVDDILDTKLEQQ-FKLVMDKGTLDAIGLHPDGPVKRVM--YWDSVSKLVAPGGI 274
Query: 567 FVCISLLQSHILAKLME 617
V S +H +L+E
Sbjct: 275 LVITSC--NHTKDELVE 289
>At1g73600.2 68414.m08520 phosphoethanolamine N-methyltransferase 3,
putative (NMT3) strong similarity to SP|Q9FR44
Phosphoethanolamine N-methyltransferase 1 (EC 2.1.1.103)
(PEAMT 1) (AtNMT1) {Arabidopsis thaliana}; identical to
SP|Q9C6B9 Putative phosphoethanolamine
N-methyltransferase 3 {Arabidopsis thaliana}
Length = 376
Score = 35.9 bits (79), Expect = 0.023
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +3
Query: 333 VDVSEVVIKQMKNVNKARTNMKFMCMDAT--RMSFEXEAFNVVLXKGTLDALMPDATE 500
VD E VIK+ +N+N N+KF+C D T M+F E+ +++ L L E
Sbjct: 94 VDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSNWLLMYLSDQEVE 151
>At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase 3,
putative (NMT3) strong similarity to SP|Q9FR44
Phosphoethanolamine N-methyltransferase 1 (EC 2.1.1.103)
(PEAMT 1) (AtNMT1) {Arabidopsis thaliana}; identical to
SP|Q9C6B9 Putative phosphoethanolamine
N-methyltransferase 3 {Arabidopsis thaliana}
Length = 555
Score = 35.9 bits (79), Expect = 0.023
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +3
Query: 333 VDVSEVVIKQMKNVNKARTNMKFMCMDAT--RMSFEXEAFNVVLXKGTLDALMPDATE 500
VD E VIK+ +N+N N+KF+C D T M+F E+ +++ L L E
Sbjct: 145 VDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSNWLLMYLSDQEVE 202
>At2g45300.1 68415.m05638 3-phosphoshikimate
1-carboxyvinyltransferase /
5-enolpyruvylshikimate-3-phosphate / EPSP synthase
nearly identical to SP|P05466
Length = 520
Score = 32.3 bits (70), Expect = 0.29
Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 5/71 (7%)
Frame = +3
Query: 105 MNLLPRSHKEFSEKDYWNKFFXKRGNKAFEWYGEYLELCGQLNKYIK-----TKDSILIT 269
+ L+ R D W++FF K G K Y+E Y T +++ +
Sbjct: 290 LKLMERFGVSVEHSDSWDRFFVKGGQKYKSPGNAYVEGDASSASYFLAGAAITGETVTVE 349
Query: 270 GCGNSSLSADL 302
GCG +SL D+
Sbjct: 350 GCGTTSLQGDV 360
>At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family protein
similar to ubiquinone biosynthesis methyltransferase
COQ5 [Saccharomyces cerevisiae][SP|P49017],
ubiquinone/menaquinone biosynthesis methyltransferase
ubiE [Escherichia coli][SP|P27851]; contains Pfam
profile PF01209: methlytransferase, UbiE/COQ5 family
Length = 288
Score = 30.7 bits (66), Expect = 0.88
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +3
Query: 516 IXKYFSEIKRVLKLGGRFVCISLLQSHI 599
I K +E RVLK GGRF+C+ L SH+
Sbjct: 186 IEKALAEAYRVLKRGGRFLCLEL--SHV 211
>At1g48860.2 68414.m05470 3-phosphoshikimate
1-carboxyvinyltransferase, putative /
5-enolpyruvylshikimate-3-phosphate, putative / EPSP
synthase, putative strong similarity to
5-enolpyruvylshikimate-3-phosphate (EPSP) synthase
SP|P05466 from (Arabidopsis thaliana)
Length = 489
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/71 (25%), Positives = 30/71 (42%), Gaps = 5/71 (7%)
Frame = +3
Query: 105 MNLLPRSHKEFSEKDYWNKFFXKRGNKAFEWYGEYLELCGQLNKYIK-----TKDSILIT 269
+ L+ R + W++FF K G K Y+E Y T +++ +
Sbjct: 234 LKLMERFGVSAEHSESWDRFFVKGGQKYKSPGNAYVEGDASSASYFLAGAAITGETVTVE 293
Query: 270 GCGNSSLSADL 302
GCG +SL D+
Sbjct: 294 GCGTTSLQGDV 304
>At1g48860.1 68414.m05471 3-phosphoshikimate
1-carboxyvinyltransferase, putative /
5-enolpyruvylshikimate-3-phosphate, putative / EPSP
synthase, putative strong similarity to
5-enolpyruvylshikimate-3-phosphate (EPSP) synthase
SP|P05466 from (Arabidopsis thaliana)
Length = 521
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/71 (25%), Positives = 30/71 (42%), Gaps = 5/71 (7%)
Frame = +3
Query: 105 MNLLPRSHKEFSEKDYWNKFFXKRGNKAFEWYGEYLELCGQLNKYIK-----TKDSILIT 269
+ L+ R + W++FF K G K Y+E Y T +++ +
Sbjct: 291 LKLMERFGVSAEHSESWDRFFVKGGQKYKSPGNAYVEGDASSASYFLAGAAITGETVTVE 350
Query: 270 GCGNSSLSADL 302
GCG +SL D+
Sbjct: 351 GCGTTSLQGDV 361
>At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase 1
/ PEAMT 1 (NMT1) identical to Phosphoethanolamine
N-methyltransferase 1 (EC 2.1.1.103) (PEAMT 1) (AtNMT1)
(SP:Q9FR44){Arabidopsis thaliana}; strong similarity to
phosphoethanolamine N-methyltransferase from [Spinacia
oleracea] GI:7407189, [Triticum aestivum] GI:17887465;
contains Pfam profile PF01209: methlytransferase,
UbiE/COQ5 family
Length = 491
Score = 29.9 bits (64), Expect = 1.5
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +3
Query: 255 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 419
S+L G G + +L I +D + VIK+ +++N N+KFMC D T
Sbjct: 56 SVLELGAGIGRFTGELAQKAGELIA-LDFIDNVIKKNESINGHYKNVKFMCADVT 109
>At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 491
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 255 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 419
S+L G G + +L + +D E I++ ++VN N+KFMC D T
Sbjct: 56 SVLELGAGIGRFTGELAQKAG-EVIALDFIESAIQKNESVNGHYKNIKFMCADVT 109
>At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 475
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 255 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 419
S+L G G + +L + +D E I++ ++VN N+KFMC D T
Sbjct: 40 SVLELGAGIGRFTGELAQKAG-EVIALDFIESAIQKNESVNGHYKNIKFMCADVT 93
>At4g20470.1 68417.m02986 hypothetical protein
Length = 140
Score = 28.7 bits (61), Expect = 3.5
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = -3
Query: 409 MHINFILVLALFTFFICLITTS--ETSTFVIFTYPTSYRSALKLELPQPVISIESLVFIY 236
+H F+L+ +L TFF +S S F F+ + RS L L P SI ++ ++
Sbjct: 50 LHCTFLLLSSLLTFFFFFRFSSIFSLSLFFSFSQKSLLRSCLSLNNVAP-FSISTITQLF 108
Query: 235 LF 230
F
Sbjct: 109 FF 110
>At5g43890.1 68418.m05367 flavin-containing monooxygenase family
protein / FMO family protein similar to
flavin-containing monooxygenases YUCCA [gi:16555352],
YUCCA2 [gi:16555354], and YUCCA3 [gi:16555356] from
Arabidopsis thaliana; contains Pfam profile PF00743
Length = 424
Score = 27.9 bits (59), Expect = 6.2
Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Frame = +3
Query: 192 EWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGY-VNITNVDVSEVVIKQMK 368
E+ GE + C + S+L+ GCGNS + L + N + V S V + +
Sbjct: 173 EFEGEVIHSCEYKSGEKYRGKSVLVVGCGNSGMEVSLDLANHNANASMVVRSSVHVLPRE 232
Query: 369 NVNKARTNMKFMCM 410
+ K+ + M M
Sbjct: 233 ILGKSSFEISMMLM 246
>At4g19440.1 68417.m02860 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 825
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = +3
Query: 222 GQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNV 374
G LNK I+ KD ++ G +S + + GY D +E ++K+M ++
Sbjct: 379 GSLNKAIEIKDLMVSKGLSLTSSTYNTLIKGYCKNGQADNAERLLKEMLSI 429
>At3g48290.1 68416.m05270 cytochrome P450, putative very strong
similarity to Cytochrome P450 71A24
(SP:Q9STK9)[Arabidopsis thaliana];
Length = 488
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -1
Query: 351 QLPRHPHLLYLHIQHRIGPRSNLNYHS 271
QL RHPH + HR GP L++ S
Sbjct: 48 QLGRHPHRSLCSLSHRYGPLMLLHFGS 74
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,758,549
Number of Sequences: 28952
Number of extensions: 210661
Number of successful extensions: 621
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 597
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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