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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P04_F_J03
         (643 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g52600.1 68414.m05938 signal peptidase, putative similar to S...   206   1e-53
At3g15710.1 68416.m01991 signal peptidase, putative similar to S...   198   3e-51
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica...    28   6.1  

>At1g52600.1 68414.m05938 signal peptidase, putative similar to
           SP|P13679 Microsomal signal peptidase 21 kDa subunit (EC
           3.4.-.-) {Canis familiaris}; contains Pfam profile
           PF00461: Signal peptidase I
          Length = 180

 Score =  206 bits (503), Expect = 1e-53
 Identities = 100/173 (57%), Positives = 126/173 (72%), Gaps = 1/173 (0%)
 Frame = +3

Query: 123 DDVRRMNKRQFMYQVLSFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSMEPAFHRGDLL 302
           D ++ +  RQ   Q +S GMIV+SAL+IWK LM VTGSESP+VVVLSGSMEP F RGD+L
Sbjct: 9   DSIKSIQIRQLFTQAISLGMIVTSALIIWKALMCVTGSESPVVVVLSGSMEPGFKRGDIL 68

Query: 303 FLTNYPEEPVRVGEIVVFKVXGRDIPIVHRVLKLHEK-NNGTVKFLTKGDNNSVDDRGLY 479
           FL +  ++P+R GEIVVF V GRDIPIVHRV+K+HE+ N G V  LTKGDNN  DDR LY
Sbjct: 69  FL-HMSKDPIRAGEIVVFNVDGRDIPIVHRVIKVHERENTGEVDVLTKGDNNYGDDRLLY 127

Query: 480 AQXQLWLTKKDVVGRAXXFXPYVGMVTIYMNEYPKFKFAVLACLAIYVLXHRE 638
           A+ QLWL +  ++GRA  F PYVG VTI M E P  K+ ++  L + V+  ++
Sbjct: 128 AEGQLWLHRHHIMGRAVGFLPYVGWVTIIMTEKPIIKYILIGALGLLVITSKD 180


>At3g15710.1 68416.m01991 signal peptidase, putative similar to
           SP|P13679 Microsomal signal peptidase 21 kDa subunit (EC
           3.4.-.-) {Canis familiaris}; contains Pfam profile:
           PF00461 signal peptidase I
          Length = 180

 Score =  198 bits (483), Expect = 3e-51
 Identities = 94/173 (54%), Positives = 124/173 (71%), Gaps = 1/173 (0%)
 Frame = +3

Query: 123 DDVRRMNKRQFMYQVLSFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSMEPAFHRGDLL 302
           D ++ +  RQ + Q ++ GMIV+SAL+IWK L+ VTGSESP+VVVLS SMEP F RGD+L
Sbjct: 9   DSIKSIKIRQVLTQAITLGMIVTSALIIWKALICVTGSESPVVVVLSESMEPGFQRGDIL 68

Query: 303 FLTNYPEEPVRVGEIVVFKVXGRDIPIVHRVLKLHEK-NNGTVKFLTKGDNNSVDDRGLY 479
           FL    +EP+R GEIVVF V GR+IPIVHR +K+HE+ +   V  LTKGDNN +DD GLY
Sbjct: 69  FL-RMTDEPIRAGEIVVFSVDGREIPIVHRAIKVHERGDTKAVDVLTKGDNNDIDDIGLY 127

Query: 480 AQXQLWLTKKDVVGRAXXFXPYVGMVTIYMNEYPKFKFAVLACLAIYVLXHRE 638
           A+ QLWL +  ++GRA  F PYVG VTI M E P  K+ ++  L + V+  ++
Sbjct: 128 AEGQLWLHRHHIMGRAVGFLPYVGWVTIIMTEKPIIKYILIGALGLLVITSKD 180


>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
            domain-containing protein / F-box family protein similar
            to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
            pombe}; contains Pfam profiles PF00271: Helicase
            conserved C-terminal domain, PF00176: SNF2 family
            N-terminal domain, PF00646: F-box domain
          Length = 1378

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 11/29 (37%), Positives = 19/29 (65%)
 Frame = +3

Query: 381  IVHRVLKLHEKNNGTVKFLTKGDNNSVDD 467
            +V R+ KLHE N  ++    K DN++++D
Sbjct: 1139 LVDRLRKLHEGNKKSILSFNKTDNDNLED 1167


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,708,047
Number of Sequences: 28952
Number of extensions: 270021
Number of successful extensions: 588
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 578
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 584
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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