Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P04_F_B12
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 35 0.041
At5g61390.1 68418.m07702 exonuclease family protein contains exo... 33 0.22
At5g07710.1 68418.m00884 exonuclease family protein contains exo... 31 0.51
At1g12760.1 68414.m01481 zinc finger (C3HC4-type RING finger) fa... 29 3.6
At2g47470.2 68415.m05924 thioredoxin family protein similar to p... 28 4.7
At2g47470.1 68415.m05925 thioredoxin family protein similar to p... 28 4.7
At3g05480.1 68416.m00600 cell cycle checkpoint control protein f... 28 6.2
At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR... 28 6.2
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains
Pfam profiles PF00632: HECT-domain
(ubiquitin-transferase), PF00612: IQ calmodulin-binding
motif
Length = 1142
Score = 35.1 bits (77), Expect = 0.041
Identities = 25/73 (34%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = +1
Query: 385 TETEMNXETCLQVNSTQDQLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPVGD-KL 561
TE + E V L I VL D +K I E +DA+TAK ++Q +G K
Sbjct: 165 TECNYSQERIKDVIGVNALLLRILIVLTDPKSWKIITNENFEDAETAKKIIIQFIGSCKS 224
Query: 562 NKYTMWVKYXKTL 600
Y+ +Y KTL
Sbjct: 225 GYYSAVRRYIKTL 237
>At5g61390.1 68418.m07702 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 487
Score = 32.7 bits (71), Expect = 0.22
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +1
Query: 166 VDKGSPPQWSP-VYTVKGLLNIPYAELHEP 252
VD GS W+P V +KG LN P A +H P
Sbjct: 383 VDSGSGSDWNPLVIPMKGFLNCPTARIHIP 412
>At5g07710.1 68418.m00884 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 468
Score = 31.5 bits (68), Expect = 0.51
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +1
Query: 166 VDKGSPPQWSPVYT-VKGLLNIPYAELH 246
VD GS +W+PV +KG +N P A +H
Sbjct: 365 VDSGSTSEWNPVVNPMKGFVNYPNARIH 392
>At1g12760.1 68414.m01481 zinc finger (C3HC4-type RING finger)
family protein low similarity to SP|Q9NVW2 RING finger
protein 12 (LIM domain interacting RING finger protein)
{Homo sapiens}; contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 368
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 223 LGVPLLCILDSIVVATLYRHIQMRRPRKNTTT 128
LG L C+L + V YR RR + TTT
Sbjct: 136 LGYALQCVLHMVCVCVEYRRRNRRRTNRTTTT 167
>At2g47470.2 68415.m05924 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 266
Score = 28.3 bits (60), Expect = 4.7
Identities = 14/25 (56%), Positives = 19/25 (76%), Gaps = 3/25 (12%)
Frame = +3
Query: 333 VSGLPTLRYFYKD-RSGH--NGNRD 398
VSG PTL++F KD ++GH +G RD
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRD 237
>At2g47470.1 68415.m05925 thioredoxin family protein similar to
protein disulfide isomerase [Dictyostelium discoideum]
GI:2627440; contains Pfam profile: PF00085 Thioredoxin
Length = 361
Score = 28.3 bits (60), Expect = 4.7
Identities = 14/25 (56%), Positives = 19/25 (76%), Gaps = 3/25 (12%)
Frame = +3
Query: 333 VSGLPTLRYFYKD-RSGH--NGNRD 398
VSG PTL++F KD ++GH +G RD
Sbjct: 213 VSGFPTLKFFPKDNKAGHDYDGGRD 237
>At3g05480.1 68416.m00600 cell cycle checkpoint control protein
family contains Pfam profile PF04139: Rad9; contains
Prosite PS00976: Myristoyl-CoA:protein
N-myristoyltransferase signature 2; similar to
radio-resistance/chemo-resistance/cell cycle checkpoint
control protein (GI:3869274) [Mus musculus]
Length = 439
Score = 27.9 bits (59), Expect = 6.2
Identities = 26/110 (23%), Positives = 40/110 (36%), Gaps = 4/110 (3%)
Frame = +1
Query: 265 YDSKNSKSRIDYYGGMVKTYQFTSAVYPP-YGTSIKIAPVTTETEMNXETCLQ-VNSTQD 438
+D+ K + Y GM KTY T V P S+ + M+ + + + Q
Sbjct: 98 HDASKVKWTLQCYSGMKKTYWITCNVEPDIQHLSLDRGRFPSTLVMHPRNLSKLLGNFQS 157
Query: 439 QLQDIQSVLPDMTDFKYIGTETMQDADTAKWQMVQPV--GDKLNKYTMWV 582
LQ+I + D T F + V P GD L +W+
Sbjct: 158 SLQEITIIATDQTSFPSDAASEIGGKAVEFRSYVDPTKDGDSLLHTQLWI 207
>At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1109
Score = 27.9 bits (59), Expect = 6.2
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +2
Query: 233 MLNCMSHSMLGTTVRTANQESITMEVWSRLTSSRQRSTHPTVLL 364
M NC+S ++ T + A+ E ITM SRL + ST+ LL
Sbjct: 677 MANCISLEVIPTHINLASLEHITMTGCSRLKTFPDFSTNIERLL 720
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,175,639
Number of Sequences: 28952
Number of extensions: 298526
Number of successful extensions: 750
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 750
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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