Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_J20
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g26830.1 68414.m03270 cullin, putative similar to Cullin homo... 136 2e-32
At1g69670.1 68414.m08018 cullin, putative contains similarity to... 131 5e-31
At1g02980.1 68414.m00268 cullin family protein similar to cullin... 126 2e-29
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 120 7e-28
At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 C... 112 2e-25
At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3, ubiqu... 36 0.035
At5g28400.1 68418.m03448 expressed protein 33 0.24
At1g26290.1 68414.m03208 hypothetical protein 32 0.43
At1g17970.1 68414.m02223 zinc finger (C3HC4-type RING finger) fa... 31 0.56
At3g59410.1 68416.m06626 protein kinase family protein low simil... 29 3.0
At4g25450.1 68417.m03665 ABC transporter family protein similar ... 29 4.0
At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein heli... 29 4.0
At1g17680.2 68414.m02189 transcription factor-related low simila... 29 4.0
At1g17680.1 68414.m02188 transcription factor-related low simila... 29 4.0
At5g42710.1 68418.m05202 hypothetical protein 28 6.9
At5g52050.1 68418.m06460 MATE efflux protein-related contains Pf... 27 9.2
At5g19410.1 68418.m02313 ABC transporter family protein white me... 27 9.2
At3g56660.1 68416.m06301 bZIP transcription factor family protei... 27 9.2
At2g47960.1 68415.m05999 expressed protein 27 9.2
>At1g26830.1 68414.m03270 cullin, putative similar to Cullin homolog 3
(CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens];
contains Pfam profile PF00888: Cullin family
Length = 732
Score = 136 bits (328), Expect = 2e-32
Identities = 79/222 (35%), Positives = 128/222 (57%), Gaps = 5/222 (2%)
Frame = -2
Query: 691 LPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKN-RYTLQASTFQMAVLLQ 515
LP E +F ++Y H+GR+L+W NM ++ K ++ L STFQM VL+
Sbjct: 511 LPAEVSVLCEKFRSYYLGTHTGRRLSWQTNMGTADIKAIFGKGQKHELNVSTFQMCVLML 570
Query: 514 YNDNTSWTVRQLEQHTGIKGDFLIQVLQIL--LKAKLLVCQEDEA-ELGENSVVDLYSGY 344
+N++ + +++EQ T I L + LQ L +K K ++ +E + ++GE + + +
Sbjct: 571 FNNSDRLSYKEIEQATEIPAADLKRCLQSLACVKGKNVIKKEPMSKDIGEEDLFVVNDKF 630
Query: 343 KNKKLRVNIN-IPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEV 167
+K +V I + + E + E++ T + +EEDRK I+AAIVRIMK+RK L H +++ EV
Sbjct: 631 TSKFYKVKIGTVVAQKETEPEKQETRQRVEEDRKPQIEAAIVRIMKSRKILDHNNIIAEV 690
Query: 166 LNQLSSRFKPRVPVIKKCIDILIEKEYLERTEGEKDTYSYLA 41
QL RF IKK I+ LIE+++LER ++ Y YLA
Sbjct: 691 TKQLQPRFLANPTEIKKRIESLIERDFLERDSTDRKLYRYLA 732
>At1g69670.1 68414.m08018 cullin, putative contains similarity to
Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from [Homo
sapiens]; contains Pfam profile PF00888: Cullin family
Length = 732
Score = 131 bits (316), Expect = 5e-31
Identities = 77/222 (34%), Positives = 127/222 (57%), Gaps = 5/222 (2%)
Frame = -2
Query: 691 LPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKN-RYTLQASTFQMAVLLQ 515
LP E +F ++Y H+GR+L+W NM ++ K ++ L STFQM VL+
Sbjct: 511 LPAEVSVLCEKFRSYYLGTHTGRRLSWQTNMGTADIKAVFGKGQKHELNVSTFQMCVLML 570
Query: 514 YNDNTSWTVRQLEQHTGIKGDFLIQVLQIL--LKAKLLVCQEDEA-ELGENSVVDLYSGY 344
+N++ + +++EQ T I L + LQ + +K K ++ +E + E+ E + +
Sbjct: 571 FNNSDRLSYKEIEQATEIPTPDLKRCLQSMACVKGKNVLRKEPMSKEIAEEDWFVVNDRF 630
Query: 343 KNKKLRVNIN-IPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEV 167
+K +V I + + E + E++ T + +EEDRK I+AAIVRIMK+R+ L H +++ EV
Sbjct: 631 ASKFYKVKIGTVVAQKETEPEKQETRQRVEEDRKPQIEAAIVRIMKSRRVLDHNNIIAEV 690
Query: 166 LNQLSSRFKPRVPVIKKCIDILIEKEYLERTEGEKDTYSYLA 41
QL +RF IKK I+ LIE+++LER ++ Y YLA
Sbjct: 691 TKQLQTRFLANPTEIKKRIESLIERDFLERDNTDRKLYRYLA 732
>At1g02980.1 68414.m00268 cullin family protein similar to cullin 1
[Homo sapiens] GI:3139077; contains Pfam profile PF00888:
Cullin family
Length = 742
Score = 126 bits (303), Expect = 2e-29
Identities = 71/223 (31%), Positives = 118/223 (52%), Gaps = 2/223 (0%)
Frame = -2
Query: 703 TSFQLPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKNRYTLQASTFQMAV 524
T LP E V F +Y ++ + R+L+W+Y++ +L K + +T+Q AV
Sbjct: 524 TDLNLPIEMVNCVEAFKAYYGTKTNSRRLSWIYSLGTCQLAGKFDKKTIEIVVTTYQAAV 583
Query: 523 LLQYNDNTSWTVRQLEQHTGIKGDFLIQVLQIL--LKAKLLVCQEDEAELGENSVVDLYS 350
LL +N+ + ++ + + + L ++L L LK K+L+ + + + S
Sbjct: 584 LLLFNNTERLSYTEILEQLNLGHEDLARLLHSLSCLKYKILIKEPMSRNISNTDTFEFNS 643
Query: 349 GYKNKKLRVNINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVE 170
+ +K R+ + +P E K E +++DR+ I AA+VRIMK+RK L HQ LV E
Sbjct: 644 KFTDKMRRIRVPLPPMDERKKIVE----DVDKDRRYAIDAALVRIMKSRKVLGHQQLVSE 699
Query: 169 VLNQLSSRFKPRVPVIKKCIDILIEKEYLERTEGEKDTYSYLA 41
+ LS FKP + +IKK I+ LI ++YLER +T+ YLA
Sbjct: 700 CVEHLSKMFKPDIKMIKKRIEDLISRDYLERDTDNPNTFKYLA 742
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile PF00888:
Cullin family
Length = 738
Score = 120 bits (290), Expect = 7e-28
Identities = 72/219 (32%), Positives = 117/219 (53%), Gaps = 2/219 (0%)
Frame = -2
Query: 691 LPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKNRYTLQASTFQMAVLLQY 512
LP+E + V F FY ++ RKL W+Y++ + + L ST+Q AVLL +
Sbjct: 524 LPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLF 583
Query: 511 NDNTSWTVRQLEQHTGIKGDFLIQVLQIL--LKAKLLVCQEDEAELGENSVVDLYSGYKN 338
N + ++ + + L+++L L K K+L+ + + + +N + S + +
Sbjct: 584 NTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTD 643
Query: 337 KKLRVNINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEVLNQ 158
+ R+ I +P E K E +++DR+ I AAIVRIMK+RK L HQ LV E + Q
Sbjct: 644 RMRRIKIPLPPVDERKKVVE----DVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQ 699
Query: 157 LSSRFKPRVPVIKKCIDILIEKEYLERTEGEKDTYSYLA 41
LS FKP + IKK ++ LI ++YLER + + + YLA
Sbjct: 700 LSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738
>At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 Cullin
homolog 4A (CUL-4A) {Homo sapiens}; contains Pfam profile
PF00888: Cullin family
Length = 792
Score = 112 bits (270), Expect = 2e-25
Identities = 72/220 (32%), Positives = 110/220 (50%), Gaps = 2/220 (0%)
Frame = -2
Query: 694 QLPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKNRYTLQASTFQMAVLLQ 515
+LP E F FY S++SGR+L W ++ L + K + L S FQ VL+
Sbjct: 575 KLPHELNVYQDIFKEFYLSKYSGRRLMWQNSLGHCVLKADFSKGKKELAVSLFQAVVLML 634
Query: 514 YNDNTSWTVRQLEQHTGIKGDFLIQVLQILLKAKLLVCQEDEA--ELGENSVVDLYSGYK 341
+ND + ++ T I+ L + LQ L K+ V Q++ ++ + + +
Sbjct: 635 FNDAMKLSFEDIKDSTSIEDKELRRTLQSLACGKVRVLQKNPKGRDVEDGDEFEFNDEFA 694
Query: 340 NKKLRVNINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEVLN 161
R+ +N E E +T + + +DR+ I AAIVRIMKTRK L H L+ E+
Sbjct: 695 APLYRIKVNAIQMKETVEENTSTTERVFQDRQYQIDAAIVRIMKTRKVLSHTLLITELFQ 754
Query: 160 QLSSRFKPRVPVIKKCIDILIEKEYLERTEGEKDTYSYLA 41
QL KP +KK I+ LI++EYLER + Y+YLA
Sbjct: 755 QLKFPIKP--ADLKKRIESLIDREYLEREKSNPQIYNYLA 792
>At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3,
ubiquitin ligase; contains similarity to
anaphase-promoting complex subunit 2 GI:6180009 from
[Homo sapiens]
Length = 865
Score = 35.5 bits (78), Expect = 0.035
Identities = 20/83 (24%), Positives = 35/83 (42%)
Frame = -2
Query: 694 QLPTEXERSVHRFTTFYSSQHSGRKLNWLYNMSKGELLTNCFKNRYTLQASTFQMAVLLQ 515
+LP ++ + + Y + RKL W N+ +L S A+++Q
Sbjct: 651 ELPGPVDKLLSDYANRYHEIKTPRKLLWKKNLGTVKLELQFEDRAMQFTVSPTHAAIIMQ 710
Query: 514 YNDNTSWTVRQLEQHTGIKGDFL 446
+ + SWT + L + GI D L
Sbjct: 711 FQEKKSWTYKDLAEVIGIPIDAL 733
>At5g28400.1 68418.m03448 expressed protein
Length = 996
Score = 32.7 bits (71), Expect = 0.24
Identities = 23/85 (27%), Positives = 41/85 (48%)
Frame = -2
Query: 394 DEAELGENSVVDLYSGYKNKKLRVNINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRI 215
D AEL + D K++KLR + EL ++ +H ED+ + +Q+ ++
Sbjct: 600 DIAELYRSEYNDELEWMKDEKLRDIVFCVRDNEL-AGRDPSHLIDAEDKAIFLQSLEKKV 658
Query: 214 MKTRKTLKHQHLVVEVLNQLSSRFK 140
K + L H H V + L ++ R+K
Sbjct: 659 EKENEKLSHLHHVYDPLEKIIPRWK 683
>At1g26290.1 68414.m03208 hypothetical protein
Length = 142
Score = 31.9 bits (69), Expect = 0.43
Identities = 19/79 (24%), Positives = 38/79 (48%)
Frame = -2
Query: 484 QLEQHTGIKGDFLIQVLQILLKAKLLVCQEDEAELGENSVVDLYSGYKNKKLRVNINIPL 305
++E ++G+F + + LK + L Q E E + D+ + L VNINI +
Sbjct: 29 EMEIERNLEGEFKDGIYNLALKLRRLYEQRRERE----ELFDVSMRKSKRVLEVNINIKM 84
Query: 304 KTELKVEQEATHKHIEEDR 248
+ + K+E K ++ ++
Sbjct: 85 EGDTKIEITEKKKEVDNEK 103
>At1g17970.1 68414.m02223 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 368
Score = 31.5 bits (68), Expect = 0.56
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +2
Query: 521 QHRHLERARLQRVPVLEAVGEEFPLAHVVXPV 616
+H L RAR Q + L A ++ PL+HVV P+
Sbjct: 8 EHIRLRRARNQTIRHLHAADDDPPLSHVVLPI 39
>At3g59410.1 68416.m06626 protein kinase family protein low
similarity to GCN2 eIF2alpha kinase [Mus musculus]
GI:6066585; contains Pfam profiles PF03129: Anticodon
binding domain, PF00069: Protein kinase domain
Length = 1241
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/43 (34%), Positives = 25/43 (58%)
Frame = -2
Query: 304 KTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLV 176
K +L Q A K +D+++ + + IVR + T L+HQH+V
Sbjct: 443 KNKLDGRQYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVV 485
>At4g25450.1 68417.m03665 ABC transporter family protein similar to
multidrug resistance protein 2 SP:P21440 from [Mus
musculus]
Length = 714
Score = 28.7 bits (61), Expect = 4.0
Identities = 19/71 (26%), Positives = 39/71 (54%), Gaps = 2/71 (2%)
Frame = -2
Query: 358 LYSGYKNKKLRVNINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTL--KHQ 185
L SG + +++ + ++ + + EAT ++ + L+Q+A+ R+MK R TL H+
Sbjct: 610 LLSGGQRQRVAIARSLLKNAPILILDEATSA-LDAVSERLVQSALNRLMKDRTTLVIAHR 668
Query: 184 HLVVEVLNQLS 152
V+ NQ++
Sbjct: 669 LSTVQSANQIA 679
>At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein helicase,
putative
Length = 2172
Score = 28.7 bits (61), Expect = 4.0
Identities = 21/86 (24%), Positives = 37/86 (43%)
Frame = +2
Query: 416 RLQ*YLKHLYEEVPFDAGVLLQLADRPRGVVVVLQQHRHLERARLQRVPVLEAVGEEFPL 595
+LQ YL+ + E++P ++ + +LAD+ +V+ E L P
Sbjct: 900 KLQYYLRLMNEQLPIESQFISKLADQLNAEIVLGTIQNAREACHWLGYTYLYVRMVRNPT 959
Query: 596 AHVVXPVQLAPGVLRRVERGEPVHGA 673
+ V P LA +L R + +H A
Sbjct: 960 LYGVSPDALAKDLLLEERRADLIHSA 985
>At1g17680.2 68414.m02189 transcription factor-related low
similarity to SP|P33339 Transcription factor tau 131 kDa
subunit (TFIIIC 131 kDa subunit) Saccharomyces
cerevisiae, transcription factor IIIC102 short isoform
[Homo sapiens] GI:18481637
Length = 896
Score = 28.7 bits (61), Expect = 4.0
Identities = 14/58 (24%), Positives = 29/58 (50%)
Frame = -2
Query: 322 NINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEVLNQLSS 149
N+ L + LK+ Q H H+EE M +++ I+ +H L+ + ++L++
Sbjct: 356 NVGKELSSSLKIRQAICHVHLEE---MEQAESVLSILPQEAVSEHPELITNLADELTN 410
>At1g17680.1 68414.m02188 transcription factor-related low
similarity to SP|P33339 Transcription factor tau 131 kDa
subunit (TFIIIC 131 kDa subunit) Saccharomyces
cerevisiae, transcription factor IIIC102 short isoform
[Homo sapiens] GI:18481637
Length = 896
Score = 28.7 bits (61), Expect = 4.0
Identities = 14/58 (24%), Positives = 29/58 (50%)
Frame = -2
Query: 322 NINIPLKTELKVEQEATHKHIEEDRKMLIQAAIVRIMKTRKTLKHQHLVVEVLNQLSS 149
N+ L + LK+ Q H H+EE M +++ I+ +H L+ + ++L++
Sbjct: 356 NVGKELSSSLKIRQAICHVHLEE---MEQAESVLSILPQEAVSEHPELITNLADELTN 410
>At5g42710.1 68418.m05202 hypothetical protein
Length = 807
Score = 27.9 bits (59), Expect = 6.9
Identities = 35/137 (25%), Positives = 60/137 (43%), Gaps = 1/137 (0%)
Frame = -2
Query: 415 KLLVCQEDEAELGENSVV-DLYSGYKNKKLRVNINIPLKTELKVEQEATHKHIEEDRKML 239
K++ E + E G++ ++ Y+ KK V+ I VE + K I++D +L
Sbjct: 503 KVMKRPEIKKEDGKHDLLLKSYNDSNEKKAEVDTCIKSSQVSGVEHK---KEIKDDSILL 559
Query: 238 IQAAIVRIMKTRKTLKHQHLVVEVLNQLSSRFKPRVPVIKKCIDILIEKEYLERTEGEKD 59
I A V + H + ++Q + +P DIL + Y E T+GE +
Sbjct: 560 IAAERVPCQAPSENQHHGRMFTNGMDQQAP-----IPKSDGNSDILSKTVYKE-TKGEIE 613
Query: 58 TYSYLA*SRQRRRKKDS 8
L RQ RRK+++
Sbjct: 614 AGLPLLEKRQERRKRET 630
>At5g52050.1 68418.m06460 MATE efflux protein-related contains Pfam
profile PF01554: Uncharacterized membrane protein family
Length = 505
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/43 (27%), Positives = 26/43 (60%)
Frame = +3
Query: 216 IRTMAACISIFLSSSMCLCVASCSTLSSVFNGMFIFTRSFLFL 344
++ ++ +S+FL+ ++ +C S V G+F++ RSF+ L
Sbjct: 22 LKNHSSVLSVFLNEAISICKISYPL---VLTGLFLYVRSFVSL 61
>At5g19410.1 68418.m02313 ABC transporter family protein white
membrane transporter, Bactrocera tryoni, EMBL:U97104
Length = 624
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/49 (30%), Positives = 28/49 (57%)
Frame = +3
Query: 192 FRVLRVFIIRTMAACISIFLSSSMCLCVASCSTLSSVFNGMFIFTRSFL 338
F VL V++I MA+ + +FLS+ ++ S + +V F+F+ F+
Sbjct: 482 FFVLCVWLIILMASSLVLFLSAVSPDFISGNSLICTVLGAFFLFSGYFI 530
>At3g56660.1 68416.m06301 bZIP transcription factor family protein
similar to AtbZIP transcription factor GI:17065880 from
[Arabidopsis thaliana]; contains Pfam profile: PF00170
bZIP transcription factor
Length = 620
Score = 27.5 bits (58), Expect = 9.2
Identities = 22/70 (31%), Positives = 30/70 (42%), Gaps = 7/70 (10%)
Frame = -1
Query: 371 LGCRSLLGVQEQKAAREYKHT-VEDGA*S*TRGY------TQTH*GGQKDAYTGRHSTDN 213
L C L G + EYK V DG +RG ++ H GG D GR+ ++
Sbjct: 296 LFCMFLFGALVNISYGEYKSNYVTDGVYDQSRGRVLVVDSSRVHCGGDSDQGVGRNVSET 355
Query: 212 ENAQDPEASA 183
EN P S+
Sbjct: 356 ENLGPPRNSS 365
>At2g47960.1 68415.m05999 expressed protein
Length = 442
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = -1
Query: 218 DNENAQDPEASALSGGSPQPVVVTVQAQSSCHQEMYR 108
+ ++ +DP S LSG P+P V+ +++ H +Y+
Sbjct: 245 NEDSTEDPPTSGLSGLIPKPPVI-IRSGGGIHNYLYK 280
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,655,471
Number of Sequences: 28952
Number of extensions: 309143
Number of successful extensions: 1113
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1060
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1102
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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