Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32162
(411 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g20970.1 68414.m02625 adhesin-related contains TIGRFAM TIGR01... 33 0.075
At5g05560.1 68418.m00604 E3 ubiquitin ligase, putative E3, ubiqu... 31 0.23
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 31 0.30
At4g21670.1 68417.m03139 double-stranded RNA-binding domain (DsR... 31 0.40
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 31 0.40
At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 30 0.53
At4g28180.1 68417.m04040 expressed protein 30 0.53
At5g44610.1 68418.m05466 DREPP plasma membrane polypeptide-relat... 30 0.70
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 29 1.6
At5g38560.1 68418.m04662 protein kinase family protein contains ... 28 2.8
At1g70340.1 68414.m08092 expressed protein 28 2.8
At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family... 27 3.7
At2g46310.1 68415.m05760 AP2 domain-containing transcription fac... 27 3.7
At4g02480.1 68417.m00335 AAA-type ATPase family protein contains... 27 4.9
At2g29870.1 68415.m03627 major intrinsic family protein / MIP fa... 27 4.9
At1g26150.1 68414.m03192 protein kinase family protein similar t... 27 4.9
At1g78650.1 68414.m09166 expressed protein weak similarity to DN... 27 6.5
At1g15030.1 68414.m01796 expressed protein 27 6.5
At1g12080.2 68414.m01397 expressed protein 27 6.5
At1g22450.1 68414.m02806 cytochrome c oxidase subunit 6b, putati... 26 8.6
At1g19680.1 68414.m02453 expressed protein 26 8.6
>At1g20970.1 68414.m02625 adhesin-related contains TIGRFAM
TIGR01612: reticulocyte binding protein; contains
TIGRFAM TIGR00864: polycystin cation channel protein;
similar to fimbriae-associated protein Fap1
[Streptococcus parasanguinis] (GI:3929312)
Length = 1498
Score = 33.1 bits (72), Expect = 0.075
Identities = 30/97 (30%), Positives = 48/97 (49%), Gaps = 4/97 (4%)
Frame = +3
Query: 57 PLDVAESAPVALDEVAPEVPSEI-KAADVIETA---SIPASESSPIVASETVLGITSEAL 224
P+ V E PV+++ P V SE+ +++DV T+ + AS+ + S TV + SE
Sbjct: 293 PVTVLE--PVSVENGHPPVESELERSSDVPFTSVAEKVNASDGEVLPDSGTVDVVVSEVS 350
Query: 225 SDVPLEIPVPVAELEQVQPTEXPTVVXSETLAKPAEE 335
SDVP E A QP+ +VV + +E+
Sbjct: 351 SDVPAETQALNAISLDSQPSGKDSVVENGNSKSESED 387
>At5g05560.1 68418.m00604 E3 ubiquitin ligase, putative E3,
ubiquitin ligase; contains similarity to Apc1/Tsg24
protein, the largest subunit of human anaphase-promoting
complex (APC/C) GI:11967711 from [Homo sapiens]
Length = 1678
Score = 31.5 bits (68), Expect = 0.23
Identities = 23/79 (29%), Positives = 34/79 (43%)
Frame = +3
Query: 126 KAADVIETASIPASESSPIVASETVLGITSEALSDVPLEIPVPVAELEQVQPTEXPTVVX 305
+A +V + +P S SPI+ S +L E + P PVA + + + P +
Sbjct: 143 QAGEVNPPSHVPFSPVSPILGSREMLRQRKEVGNSSPQNFHSPVAH-DLISKRDMPCMSS 201
Query: 306 SETLAKPAEEPSIPLVAEE 362
L P EEP P EE
Sbjct: 202 HLILRDPLEEPG-PTYVEE 219
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 31.1 bits (67), Expect = 0.30
Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 6/74 (8%)
Frame = +3
Query: 39 DAGETVPLDVAE----SAPVALDEVAP--EVPSEIKAADVIETASIPASESSPIVASETV 200
D T+P VAE +APV+ ++ P EV E +A S+ E +V +E V
Sbjct: 25 DKEVTIPTPVAEKEEVAAPVSDEKAVPEKEVTPEKEAPAAEAEKSVSVKEEETVVVAEKV 84
Query: 201 LGITSEALSDVPLE 242
+ +T+E + LE
Sbjct: 85 VVLTAEEVQKKALE 98
Score = 27.1 bits (57), Expect = 4.9
Identities = 20/73 (27%), Positives = 30/73 (41%), Gaps = 6/73 (8%)
Frame = +3
Query: 207 ITSEALSDVPLEIPVPVAELEQV-QPTEXPTVVXSETLAKPAEEPSIPL-----VAEEPA 368
+ + ++D + IP PVAE E+V P V + + E P+ V EE
Sbjct: 18 VKEKPITDKEVTIPTPVAEKEEVAAPVSDEKAVPEKEVTPEKEAPAAEAEKSVSVKEEET 77
Query: 369 SVIPXSVEALATE 407
V+ V L E
Sbjct: 78 VVVAEKVVVLTAE 90
>At4g21670.1 68417.m03139 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 981
Score = 30.7 bits (66), Expect = 0.40
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 3/99 (3%)
Frame = +3
Query: 81 PVALDEVAP-EVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSDVPLEIPVPV 257
P++ D +A EV +K A +A +PA+ P +A+ + S + VP +PV V
Sbjct: 472 PLSFDGMADTEVERRLKEAISASSAVLPAANIDPRIAAPVQFPMASASSVSVP--VPVQV 529
Query: 258 AELEQVQPTEXPTVVXSETLAKPAEEPSIP--LVAEEPA 368
+ + +QP+ S +P + SI LV EP+
Sbjct: 530 VQ-QAIQPS--AMAFPSIPFQQPQQPTSIAKHLVPSEPS 565
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 30.7 bits (66), Expect = 0.40
Identities = 32/110 (29%), Positives = 52/110 (47%), Gaps = 4/110 (3%)
Frame = +3
Query: 90 LDEVAPEVPSEIKAADVIETASI-PASESSPIVASETVLGITSEALSDVPLEIPVPVAEL 266
+ E A + EI ++D + + PAS+S+P+V E + T + E P V E+
Sbjct: 21 VSEEAKLMEKEIISSDSADVVNDKPASDSNPVVTKEEEIDQT--PAGEPEKESPAVVEEV 78
Query: 267 EQVQPTEXPT-VVXSETLAKPAEEPSI--PLVAEEPASVIPXSVEALATE 407
V E T V E K AE+ + P++ +E +V +VE + TE
Sbjct: 79 GAVVKAEESTETVKHENGEKGAEQVELKEPILVKE--TVAEVNVETVDTE 126
>At4g29060.1 68417.m04157 elongation factor Ts family protein
similar to SP|P35019 Elongation factor Ts (EF-Ts)
{Galdieria sulphuraria}; contains Pfam profiles PF00627:
UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
S1 RNA binding domain
Length = 953
Score = 30.3 bits (65), Expect = 0.53
Identities = 34/117 (29%), Positives = 47/117 (40%), Gaps = 4/117 (3%)
Frame = +3
Query: 69 AESAPVALDEVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSDVPLEIP 248
AE PV V PE + + +A+V E+ +PA +S V S + E + E
Sbjct: 362 AEKPPVETP-VEPEAEASVTSAEVEESVCVPAEVTSEEVPSSETPKVVEEEVIATKAEDD 420
Query: 249 VPVAELEQVQ----PTEXPTVVXSETLAKPAEEPSIPLVAEEPASVIPXSVEALATE 407
P E EQ + E VV K EE + A+ S EALA+E
Sbjct: 421 SPEKE-EQTETLAAAAEAEEVVPPIPETKSEEEIVENSIPPNSATDEVSSPEALASE 476
>At4g28180.1 68417.m04040 expressed protein
Length = 256
Score = 30.3 bits (65), Expect = 0.53
Identities = 21/90 (23%), Positives = 40/90 (44%), Gaps = 3/90 (3%)
Frame = +3
Query: 129 AADVIETASIPASESSPIVASETVLGITSEALS---DVPLEIPVPVAELEQVQPTEXPTV 299
+A + + A++ P + E+V + +++S +P+ +PVPV +L PTV
Sbjct: 6 SAGKVTAPPVAAAQIPPPLEEESVKEVVVQSVSVSVPIPISVPVPVPDL----VPSAPTV 61
Query: 300 VXSETLAKPAEEPSIPLVAEEPASVIPXSV 389
SE P P ++ + + SV
Sbjct: 62 SDSEAQVPLPLSPPAPEISHSKSDICSVSV 91
>At5g44610.1 68418.m05466 DREPP plasma membrane polypeptide-related
contains Pfam profile: PF05558 DREPP plasma membrane
polypeptide
Length = 168
Score = 29.9 bits (64), Expect = 0.70
Identities = 29/105 (27%), Positives = 41/105 (39%), Gaps = 8/105 (7%)
Frame = +3
Query: 72 ESAPVALDEVAPEVPSEIKAAD-VIETASIPASESSP-----IVASETVLGITSEALSDV 233
E +P + V E P E++ + V+ PA E I E + I E +
Sbjct: 17 EKSPAKKEVVEEEKPREVEVVEEVVVKTEEPAKEGETKPEEIIATGEKEIEIVEEKKEEA 76
Query: 234 -PLEIPVPVAELEQVQPTEXPTVVXSETLAKPA-EEPSIPLVAEE 362
P+E+PV A E+ E KPA EE P V E+
Sbjct: 77 KPVEVPVLAAAEEKKPAVEEEKKTAPVEEKKPAVEEEKKPAVEEK 121
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 28.7 bits (61), Expect = 1.6
Identities = 15/56 (26%), Positives = 22/56 (39%)
Frame = +3
Query: 240 EIPVPVAELEQVQPTEXPTVVXSETLAKPAEEPSIPLVAEEPASVIPXSVEALATE 407
E+PV + V TE SE + KP +I + A E + + L E
Sbjct: 415 ELPVQYGGFKTVDDTEFSNETVSEVVVKPGSSETIEIPAPETEGTLVWDIAVLGWE 470
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 27.9 bits (59), Expect = 2.8
Identities = 29/117 (24%), Positives = 38/117 (32%), Gaps = 2/117 (1%)
Frame = +3
Query: 57 PLDVAESAP--VALDEVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSD 230
P VA S P V + P P+ A + P ++SP + T +
Sbjct: 81 PPTVASSPPPPVVIASPPPSTPATTPPAPPQTVSPPPPPDASPSPPAPTTTNPPPKPSPS 140
Query: 231 VPLEIPVPVAELEQVQPTEXPTVVXSETLAKPAEEPSIPLVAEEPASVIPXSVEALA 401
P E P P E P P+ T P P P + P S P LA
Sbjct: 141 PPGETPSPPGETPS-PPKPSPSTPTPTTTTSP---PPPPATSASPPSSNPTDPSTLA 193
>At1g70340.1 68414.m08092 expressed protein
Length = 510
Score = 27.9 bits (59), Expect = 2.8
Identities = 25/95 (26%), Positives = 44/95 (46%), Gaps = 4/95 (4%)
Frame = +3
Query: 60 LDVAESAPVALDE--VAPEVPSEIKAADVIET--ASIPASESSPIVASETVLGITSEALS 227
L V + P+ LDE + P+ IK +D + S+P+ + +++++ LG +
Sbjct: 31 LQVTDIVPIDLDEKSLEPKQGFLIKISDSSHSIYVSLPSDQDDVVLSNKLQLG-QFIYVD 89
Query: 228 DVPLEIPVPVAELEQVQPTEXPTVVXSETLAKPAE 332
+ PVPV + + P P + ETL P E
Sbjct: 90 RLEPGSPVPVIKGAKPIPGRHPLLGTPETLVVPKE 124
>At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family
protein
Length = 466
Score = 27.5 bits (58), Expect = 3.7
Identities = 25/97 (25%), Positives = 38/97 (39%)
Frame = +3
Query: 81 PVALDEVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSDVPLEIPVPVA 260
P + A P+ + A TA+ + P+ ETV T+ D P+ P PV
Sbjct: 356 PQSTSNKALPAPATVTAEAESATATTTTVDK-PVPEPETVAATTTTV--DKPVPEPEPVP 412
Query: 261 ELEQVQPTEXPTVVXSETLAKPAEEPSIPLVAEEPAS 371
E V E V ++ + +P E + P P S
Sbjct: 413 EPVPVPAIE--AAVAAQVITEPTETEAKPKPHSRPLS 447
>At2g46310.1 68415.m05760 AP2 domain-containing transcription
factor, putative
Length = 294
Score = 27.5 bits (58), Expect = 3.7
Identities = 20/79 (25%), Positives = 29/79 (36%), Gaps = 3/79 (3%)
Frame = +3
Query: 69 AESAPVALDEVAPEVPSEIKAADVIETASIPASE---SSPIVASETVLGITSEALSDVPL 239
AE A + D A + + + P +E +P++ ETV G S S + L
Sbjct: 131 AEEAAIGYDRAAIRIKGHNAQTNFLTPPPSPTTEVLPETPVIDLETVSGCDSARESQISL 190
Query: 240 EIPVPVAELEQVQPTEXPT 296
P V TE T
Sbjct: 191 CSPTSVLRFSHNDETEYRT 209
>At4g02480.1 68417.m00335 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family; similar to
Spastin (Swiss-Prot:Q9UBP0) [Homo sapiens] and Spastin
(Fragment) (Swiss-Prot:Q9QYY8) [Mus musculus]; similar
to mitochondrial sorting protein 1 (MSP1) protein
(TAT-binding homolog 4) (Swiss-Prot:P28737)
[Saccharomyces cerevisiae]
Length = 1265
Score = 27.1 bits (57), Expect = 4.9
Identities = 18/68 (26%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +3
Query: 213 SEALSDVPLEIPVPVAE--LEQVQPTEXPTVVXSETLAKPAEEPSIPLVAEEPASVIPXS 386
S + S+VP+E P ++ E +P + + KP +P++ P +
Sbjct: 54 SSSASEVPIENQGPASDPGSESGEPELGSSDPQAMDAEKPVVTTDVPVMENSPETDANPE 113
Query: 387 VEALATET 410
VE LAT T
Sbjct: 114 VEVLATPT 121
>At2g29870.1 68415.m03627 major intrinsic family protein / MIP
family protein contains Pfam profile: MIP PF00230
Length = 139
Score = 27.1 bits (57), Expect = 4.9
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 230 CASRNTCSSSGTRASATD 283
CA+RNT SSSG+ S +D
Sbjct: 3 CAARNTMSSSGSSPSGSD 20
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 27.1 bits (57), Expect = 4.9
Identities = 28/97 (28%), Positives = 33/97 (34%), Gaps = 2/97 (2%)
Frame = +3
Query: 96 EVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSDVPLEIPVPVAELEQV 275
E +P P +A S P ESSP T T+ S P P P E
Sbjct: 97 EPSPPPPLPTEAPPPANPVSSPPPESSPPPPPPTEAPPTTPITSPSPPTNPPPPPESPPS 156
Query: 276 QPTEXP--TVVXSETLAKPAEEPSIPLVAEEPASVIP 380
P P + L P+ P L PAS IP
Sbjct: 157 LPAPDPPSNPLPPPKLVPPSHSPPRHL-PSPPASEIP 192
>At1g78650.1 68414.m09166 expressed protein weak similarity to DNA
polymerase delta subunit 3 (DNA polymerase delta subunit
p66) (Swiss-Prot:Q15054) [Homo sapiens]
Length = 509
Score = 26.6 bits (56), Expect = 6.5
Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +3
Query: 54 VPLDVAESAPVALD-EVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEA 221
V L +S P D +V+PE+ SE A + + + ++S +++E + I SEA
Sbjct: 340 VELSSEDSGPEKPDADVSPEIKSEEPEASKEDRQNTASVDASTTLSTEKIQAIGSEA 396
>At1g15030.1 68414.m01796 expressed protein
Length = 360
Score = 26.6 bits (56), Expect = 6.5
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -2
Query: 128 FNFTWHLRSNFI*SYGCRFSH 66
FNFT R+ SYGCR SH
Sbjct: 6 FNFTQLQRAQIDVSYGCRSSH 26
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 26.6 bits (56), Expect = 6.5
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = +3
Query: 219 ALSDVPLEIPVPVAELEQVQPTEXPTVVXSETLAKPAEEPSIPLVAEE--PASVIPXSVE 392
A +V PV V +E T TVV +E +P E S+P V E+ A ++ + E
Sbjct: 2 ATVEVEQVTPVAVENVEVPTKTVEETVVETEVTQQPEE--SVPAVTEQKSEAPIVETNEE 59
Query: 393 ALATE 407
+ E
Sbjct: 60 VVVEE 64
>At1g22450.1 68414.m02806 cytochrome c oxidase subunit 6b, putative
(COX6b) nearly identical to subunit 6b of cytochrome c
oxidase [Arabidopsis thaliana] GI:6518353
Length = 191
Score = 26.2 bits (55), Expect = 8.6
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 3/70 (4%)
Frame = +3
Query: 81 PVALDEVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEAL---SDVPLEIPV 251
PV + EVAPEV ++ A+ ++T A E SP+ + +V+ SE+ ++V E P
Sbjct: 30 PVEVKEVAPEVTTQ---AEEVKTEQ--AKEESPVEEAVSVVEEKSESAPESTEVASEAPA 84
Query: 252 PVAELEQVQP 281
+ + P
Sbjct: 85 AAEDNAEETP 94
>At1g19680.1 68414.m02453 expressed protein
Length = 444
Score = 26.2 bits (55), Expect = 8.6
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = +3
Query: 75 SAPVALDEVAPEVPSEIKAADVIETASIPASESSPIVASETVLGITSEALSDVP 236
+AP+A +A PS++ A + +SS IV+S +S ++SD+P
Sbjct: 96 TAPLAGTSLAIRSPSDVSLASPVRVEVKNIVDSSDIVSSVLPNPSSSTSVSDLP 149
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,099,588
Number of Sequences: 28952
Number of extensions: 108014
Number of successful extensions: 412
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 409
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 615542944
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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