Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0714
(680 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 140 1e-33
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 139 2e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 1e-30
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 118 3e-27
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 73 3e-13
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 67 1e-11
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 53 2e-07
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 3e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.053
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.86
SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 30 2.0
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.6
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.6
SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.1
SB_33568| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.1
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.1
SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) 28 8.0
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 140 bits (339), Expect = 1e-33
Identities = 64/87 (73%), Positives = 77/87 (88%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 427
P++TIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VL KMKETAEAYLG+ V +AVITVPAY
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAY 149
Score = 127 bits (306), Expect = 9e-30
Identities = 57/63 (90%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 243 MNP 251
+NP
Sbjct: 61 LNP 63
Score = 47.6 bits (108), Expect = 9e-06
Identities = 21/27 (77%), Positives = 25/27 (92%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKKGTGERNVLIFDL 659
+EPTAAA+AYGLDK +GE+NVLIFDL
Sbjct: 174 NEPTAAALAYGLDKSLSGEKNVLIFDL 200
Score = 35.1 bits (77), Expect = 0.053
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +1
Query: 511 NDSQRQATKDAGTISG 558
NDSQRQATKDAGTI+G
Sbjct: 151 NDSQRQATKDAGTIAG 166
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 139 bits (336), Expect = 2e-33
Identities = 61/87 (70%), Positives = 74/87 (85%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 427
P++T+FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VLTKMKETAEAYLG V +AV+TVPAY
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAY 149
Score = 132 bits (319), Expect = 3e-31
Identities = 63/68 (92%), Positives = 65/68 (95%), Gaps = 1/68 (1%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 243 MNP-NTQY 263
MNP NT +
Sbjct: 61 MNPENTVF 68
Score = 46.0 bits (104), Expect = 3e-05
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKKGTGERNVLIFDL 659
+EPTAAAIAYGLDKK ERNVLI+DL
Sbjct: 174 NEPTAAAIAYGLDKKVGVERNVLIYDL 200
Score = 33.1 bits (72), Expect = 0.21
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = +1
Query: 511 NDSQRQATKDAGTISG 558
+DSQRQATKDAGTI+G
Sbjct: 151 HDSQRQATKDAGTIAG 166
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 130 bits (314), Expect = 1e-30
Identities = 59/63 (93%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 243 MNP 251
MNP
Sbjct: 61 MNP 63
Score = 115 bits (276), Expect = 4e-26
Identities = 64/112 (57%), Positives = 74/112 (66%), Gaps = 25/112 (22%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 418
P +TIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 419 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
SSMVL KMKETAEAYLG V +AV+TVPAY
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAY 174
Score = 52.0 bits (119), Expect = 4e-07
Identities = 24/32 (75%), Positives = 28/32 (87%)
Frame = +2
Query: 413 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
++SSMVL KMKETAEAYLG V +AV+TVPAY
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAY 242
Score = 47.6 bits (108), Expect = 9e-06
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKKGTGERNVLIFDL 659
+EPTAAAIAYGLDKK ERNVLIFDL
Sbjct: 267 NEPTAAAIAYGLDKKVGQERNVLIFDL 293
Score = 34.3 bits (75), Expect = 0.093
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +1
Query: 511 NDSQRQATKDAGTISG 558
NDSQRQATKDAG ISG
Sbjct: 244 NDSQRQATKDAGVISG 259
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +1
Query: 511 NDSQRQATKDAG 546
NDSQRQATKDAG
Sbjct: 176 NDSQRQATKDAG 187
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 118 bits (285), Expect = 3e-27
Identities = 55/87 (63%), Positives = 70/87 (80%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 427
P++T+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VLTKMKETAEAYLGK V +AV+TVPAY
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAY 847
Score = 100 bits (239), Expect = 1e-21
Identities = 48/63 (76%), Positives = 55/63 (87%), Gaps = 2/63 (3%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNP-N 254
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 255 TQY 263
T +
Sbjct: 764 TVF 766
Score = 45.2 bits (102), Expect = 5e-05
Identities = 20/27 (74%), Positives = 25/27 (92%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKKGTGERNVLIFDL 659
+EPTAAAIAYG+DKK GE+N+L+FDL
Sbjct: 872 NEPTAAAIAYGMDKK-EGEKNILVFDL 897
Score = 31.9 bits (69), Expect = 0.49
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +1
Query: 511 NDSQRQATKDAGTISG 558
ND+QRQATKDAG I+G
Sbjct: 849 NDAQRQATKDAGAIAG 864
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 72.5 bits (170), Expect = 3e-13
Identities = 37/87 (42%), Positives = 54/87 (62%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 QHTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSM 427
++TI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M
Sbjct: 120 KNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAM 179
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
+ T++K TAE L V + VI+VP+Y
Sbjct: 180 LFTRLKTTAEIALKTKVTDCVISVPSY 206
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 236
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 66.9 bits (156), Expect = 1e-11
Identities = 32/68 (47%), Positives = 46/68 (67%), Gaps = 3/68 (4%)
Frame = +3
Query: 69 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 243 MNP-NTQY 263
+NP NT Y
Sbjct: 90 INPKNTIY 97
Score = 59.7 bits (138), Expect = 2e-09
Identities = 37/105 (35%), Positives = 54/105 (51%), Gaps = 1/105 (0%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSS 424
P++TI+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 425 MVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKPQKMQVPSLA 559
V+ K++ AE L TV AV++VPA +K + ++ SLA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPA-EFDIKQRNATVKAASLA 195
Score = 35.1 bits (77), Expect = 0.053
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKKGTGERNVLIFDL 659
+EPTAAA+AYGL KK G NV++ DL
Sbjct: 204 NEPTAAALAYGLHKK-EGVANVMVVDL 229
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/47 (51%), Positives = 36/47 (76%)
Frame = +2
Query: 368 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAH 48
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = +1
Query: 511 NDSQRQATKDAGTISG 558
ND+QRQATKDAGTI+G
Sbjct: 50 NDAQRQATKDAGTIAG 65
Score = 33.5 bits (73), Expect = 0.16
Identities = 17/23 (73%), Positives = 18/23 (78%), Gaps = 2/23 (8%)
Frame = +3
Query: 579 HEPTAAAIAYGLDKK--GTGERN 641
+EPTAAAIAYGLDK G ERN
Sbjct: 97 NEPTAAAIAYGLDKARGGKAERN 119
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/61 (44%), Positives = 34/61 (55%), Gaps = 3/61 (4%)
Frame = +3
Query: 132 GKVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNP-NTQYSMPNVSSDVSSKM 302
G IIAND G+R TP+ VAF+DTE+LI +V N N Q S V + + KM
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQVSPKEVINMIYKKM 142
Query: 303 L 305
L
Sbjct: 143 L 143
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 35.1 bits (77), Expect = 0.053
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = +2
Query: 362 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPF 115
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.86
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +3
Query: 141 EIIANDQGNRTTPSYVAFT-DTERL 212
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08)
Length = 1011
Score = 29.9 bits (64), Expect = 2.0
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +2
Query: 359 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKDKPQK 538
+PK K + E+ EE +M ++K LGK V+ + T ++TL + Q
Sbjct: 654 QPKFKKTKEEEEFDKLLEEADAMTQQEVKNVKNIKLGKNVKTQLYTCEPCNITLNSQKQM 713
Query: 539 MQ 544
Q
Sbjct: 714 SQ 715
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 249 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 151
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.6
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 9 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 95
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 665
Score = 28.3 bits (60), Expect = 6.1
Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +2
Query: 50 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 226
L++ N + ++ + R GY L R C R + +R + H++L R +RC
Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282
Query: 227 QEPGGDEPQHTIFDA 271
G +P++T F+A
Sbjct: 283 GTASGMDPKNTPFNA 297
>SB_33568| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1555
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/66 (25%), Positives = 32/66 (48%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 427
P H I A R++GRK +++++D +V KP + K F P+ ++S+
Sbjct: 572 PSHEIAQAWRILGRKSSRSSLESDTLE--IKVDPKDSKPTKRNILKQVASVFDPQGLASL 629
Query: 428 VLTKMK 445
+ + K
Sbjct: 630 FVLRAK 635
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -3
Query: 456 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRP 283
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
>SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14)
Length = 856
Score = 27.9 bits (59), Expect = 8.0
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = -2
Query: 259 CVLGFIATWFLAASPMRRSVSVNAT*DGVVLLPWSLAMISTFPCWKTPTQEYVVPRSIPT 80
C+L I WF P+R S + VV+ S+ ++ F C P + P +IP+
Sbjct: 107 CLLLTIERWFAVVQPLRYQASFSKR--RVVVSLISVYCVACFLCCTLPFEVSFSPENIPS 164
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,184,384
Number of Sequences: 59808
Number of extensions: 536893
Number of successful extensions: 1662
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1530
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1654
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1757375282
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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