Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0710
(718 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 140 8e-34
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 140 1e-33
At2g37160.1 68415.m04559 transducin family protein / WD-40 repea... 30 1.8
At1g20880.1 68414.m02615 RNA recognition motif (RRM)-containing ... 30 1.8
At1g06190.1 68414.m00651 expressed protein 29 2.3
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 28 5.4
At4g13650.1 68417.m02123 pentatricopeptide (PPR) repeat-containi... 28 7.1
At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, put... 28 7.1
At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase / homogen... 27 9.4
At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase / homogen... 27 9.4
At5g17970.1 68418.m02108 disease resistance protein (TIR-NBS-LRR... 27 9.4
At4g33510.1 68417.m04759 2-dehydro-3-deoxyphosphoheptonate aldol... 27 9.4
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 9.4
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
profiles PF02779: Transketolase, pyridine binding domain,
PF00676: Dehydrogenase E1 component
Length = 1017
Score = 140 bits (339), Expect = 8e-34
Identities = 68/123 (55%), Positives = 83/123 (67%)
Frame = +1
Query: 244 GFRGGYSVTNPNALVLWEAQFGDFNNVAQCIIDQFISSGQAKWVRQSGIVLLQPHGMEGM 423
GF GYS+ +PN+LVLWEAQFGDF N AQ I DQFISSG+AKW+RQ+G+V+L PHG +G
Sbjct: 712 GFELGYSMESPNSLVLWEAQFGDFANGAQVIFDQFISSGEAKWLRQTGLVMLLPHGYDGQ 771
Query: 424 GPEHSSARLERFLQMCSDDPDYMPPESPAMKCVXFTTATGS*RTVRLRRSYFXILRRQIA 603
GPEHSSARLER+LQM D+P +P P M+ +YF +LRRQI
Sbjct: 772 GPEHSSARLERYLQMSDDNPYVIPDMEPTMR-KQIQECNWQIVNATTPANYFHVLRRQIH 830
Query: 604 LPF 612
F
Sbjct: 831 RDF 833
Score = 109 bits (262), Expect = 2e-24
Identities = 54/81 (66%), Positives = 64/81 (79%), Gaps = 2/81 (2%)
Frame = +2
Query: 17 VDWALAEAMAYGSLLKEGIHVRLSGEDVERGTFSHRHHVLHHQKVDKATYCALAHLYPDQ 196
VDWALAEA+A+ +L+ EG HVRLSG+DVERGTFSHRH VLH Q+ + YC L HL +Q
Sbjct: 635 VDWALAEALAFATLVVEGNHVRLSGQDVERGTFSHRHSVLHDQETGE-EYCPLDHLIMNQ 693
Query: 197 AP--YTVCNSSLSEYGVLGFE 253
P +TV NSSLSE+GVLGFE
Sbjct: 694 DPEMFTVSNSSLSEFGVLGFE 714
Score = 43.6 bits (98), Expect = 1e-04
Identities = 20/37 (54%), Positives = 27/37 (72%), Gaps = 3/37 (8%)
Frame = +2
Query: 611 FRKPLILMTPKSLLRHPECKSS---FDDMKEGXNFQE 712
FRKPLI+M PK+LLRH +CKS+ FDD++ F +
Sbjct: 833 FRKPLIVMAPKNLLRHKDCKSNLSEFDDVQGHPGFDK 869
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
profiles PF02779: Transketolase, pyridine binding domain,
PF00676: Dehydrogenase E1 component
Length = 1025
Score = 140 bits (338), Expect = 1e-33
Identities = 67/123 (54%), Positives = 83/123 (67%)
Frame = +1
Query: 244 GFRGGYSVTNPNALVLWEAQFGDFNNVAQCIIDQFISSGQAKWVRQSGIVLLQPHGMEGM 423
GF GYS+ NPN+LV+WEAQFGDF N AQ + DQFISSG+AKW+RQ+G+V+L PHG +G
Sbjct: 716 GFELGYSMENPNSLVIWEAQFGDFANGAQVMFDQFISSGEAKWLRQTGLVVLLPHGYDGQ 775
Query: 424 GPEHSSARLERFLQMCSDDPDYMPPESPAMKCVXFTTATGS*RTVRLRRSYFXILRRQIA 603
GPEHSS RLERFLQM D+P +P P ++ V +YF +LRRQI
Sbjct: 776 GPEHSSGRLERFLQMSDDNPYVIPEMDPTLR-KQIQECNWQVVNVTTPANYFHVLRRQIH 834
Query: 604 LPF 612
F
Sbjct: 835 RDF 837
Score = 106 bits (254), Expect = 2e-23
Identities = 51/81 (62%), Positives = 62/81 (76%), Gaps = 2/81 (2%)
Frame = +2
Query: 17 VDWALAEAMAYGSLLKEGIHVRLSGEDVERGTFSHRHHVLHHQKVDKATYCALAHLYPDQ 196
+DW L EA+A+ +L+ EG HVRLSG+DVERGTFSHRH VLH Q+ + YC L HL +Q
Sbjct: 639 IDWGLGEALAFATLVVEGNHVRLSGQDVERGTFSHRHSVLHDQETGE-EYCPLDHLIKNQ 697
Query: 197 AP--YTVCNSSLSEYGVLGFE 253
P +TV NSSLSE+GVLGFE
Sbjct: 698 DPEMFTVSNSSLSEFGVLGFE 718
Score = 42.3 bits (95), Expect = 3e-04
Identities = 20/37 (54%), Positives = 26/37 (70%), Gaps = 3/37 (8%)
Frame = +2
Query: 611 FRKPLILMTPKSLLRHPECKSS---FDDMKEGXNFQE 712
FRKPLI+M PK+LLRH +C S+ FDD+K F +
Sbjct: 837 FRKPLIVMAPKNLLRHKQCVSNLSEFDDVKGHPGFDK 873
>At2g37160.1 68415.m04559 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to Dystrophia myotonica-containing WD repeat
motif protein DMR-N9 protein (DMWD) (DM9) (SP:Q08274)
[Mus musculus]; simlar to DMR protein GI:18028289 [Homo
sapiens];
Length = 544
Score = 29.9 bits (64), Expect = 1.8
Identities = 17/49 (34%), Positives = 22/49 (44%)
Frame = +2
Query: 116 SHRHHVLHHQKVDKATYCALAHLYPDQAPYTVCNSSLSEYGVLGFEAAT 262
+H +LH+ T LAHL AP T +S S GF +AT
Sbjct: 45 THPSGLLHYTHGKTVTQVTLAHLKDKPAPSTPTGTSSSSTASSGFRSAT 93
>At1g20880.1 68414.m02615 RNA recognition motif (RRM)-containing
protein similar to RRM-containing protein SEB-4 [Xenopus
laevis] GI:8895698; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM);
is the location of EST 197B1T7 , gb|AA597386
Length = 274
Score = 29.9 bits (64), Expect = 1.8
Identities = 26/93 (27%), Positives = 39/93 (41%), Gaps = 3/93 (3%)
Frame = -2
Query: 453 LQTSRGV-LGAHPLHAVRLQQYNPGLAHPLGLS--AGDELVDDALGHVVEVSELGLPQXQ 283
+Q+ RG+ G+HP H Y G+ +P G++ D + + G G Q
Sbjct: 130 VQSPRGLHFGSHPYHQPPTYNYQQGVVYPYGVTPYGPDYMYSQSQGF------YGPYMGQ 183
Query: 282 GVRVRYGVAASKPRTPYSDSELLHTV*GAWSGY 184
YGV + Y +L HT+ GA GY
Sbjct: 184 QYLQVYGVPGAVNSPGYQYGQLSHTIPGA-HGY 215
>At1g06190.1 68414.m00651 expressed protein
Length = 401
Score = 29.5 bits (63), Expect = 2.3
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = -3
Query: 233 TQTVSCCTQC--REPGRGTDVPRRSKWPCRPSGGGAR 129
T + C+ C + GR VP RS + CR S GG R
Sbjct: 34 TLAILPCSSCLDHKNGRLKSVPNRSSFVCRASSGGYR 70
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 28.3 bits (60), Expect = 5.4
Identities = 19/52 (36%), Positives = 23/52 (44%), Gaps = 5/52 (9%)
Frame = -1
Query: 508 RGFPGACSPGRRSTSARTSPNE-----PRSARGPSPPCREAAAVQSRTGAPT 368
R G+ SP R STS R + P SAR PSP + R +PT
Sbjct: 143 RSSKGSASPNRLSTSPRADNMQQIRGRPSSARHPSPASGRRSGTPVRRISPT 194
>At4g13650.1 68417.m02123 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 1064
Score = 27.9 bits (59), Expect = 7.1
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 92 EDVERGTFSHRHHVLHHQKVDKATYCALAHLY 187
+++E G F+ HH+L + D ATY L++LY
Sbjct: 875 KNMEIGEFA-AHHLLELEPEDSATYVLLSNLY 905
>At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase,
putative similar to glycerol-3-phosphate dehydrogenase
GB:BAA08926 from Mus musculus [SP|Q64521], Homo sapiens
[GI:1020315], Rattus norvegicus [SP|P35571]; contains
Pfam profile PF01266 FAD dependent oxidoreductase
Length = 629
Score = 27.9 bits (59), Expect = 7.1
Identities = 15/66 (22%), Positives = 31/66 (46%)
Frame = +1
Query: 271 NPNALVLWEAQFGDFNNVAQCIIDQFISSGQAKWVRQSGIVLLQPHGMEGMGPEHSSARL 450
NP + + ++ + ++A+ +D I SGQ K + LQ G G P +
Sbjct: 428 NPGLVTITGGKWTTYRSMAEDAVDAAIKSGQLKPTNECVTQKLQLLGSYGWEPSSFTTLA 487
Query: 451 ERFLQM 468
+++++M
Sbjct: 488 QQYVRM 493
>At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 27.5 bits (58), Expect = 9.4
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = -2
Query: 516 LHSGAFRGHVVRVVGAHLQEPLQTSRGVLGAHPLHAVR 403
L G RG+V + GAH Q P G +GA+ L A R
Sbjct: 198 LPDGKSRGYVAEIYGAHFQLP---DLGPIGANGLAASR 232
>At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 27.5 bits (58), Expect = 9.4
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = -2
Query: 516 LHSGAFRGHVVRVVGAHLQEPLQTSRGVLGAHPLHAVR 403
L G RG+V + GAH Q P G +GA+ L A R
Sbjct: 198 LPDGKSRGYVAEIYGAHFQLP---DLGPIGANGLAASR 232
>At5g17970.1 68418.m02108 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 780
Score = 27.5 bits (58), Expect = 9.4
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +2
Query: 89 GEDVERGTFSHRHHVLHHQKVD 154
GEDV R SH H L H +D
Sbjct: 24 GEDVRRNFLSHLHKELQHNGID 45
>At4g33510.1 68417.m04759 2-dehydro-3-deoxyphosphoheptonate aldolase
2 / 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
2 / DAHP synthetase 2 (DHS2) nearly identical to
SP|Q00218
Length = 507
Score = 27.5 bits (58), Expect = 9.4
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -2
Query: 384 GLAHPLGLSAGDELVDDALGHVVEV-SELGLPQXQGVRVRYGVAASKPRTP 235
G+A+PLG+ D++V L ++E+ + P V VR G + + P
Sbjct: 329 GIANPLGIKVSDKMVPSELVKLIEILNPQNKPGRITVIVRMGAENMRVKLP 379
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.5 bits (58), Expect = 9.4
Identities = 21/52 (40%), Positives = 25/52 (48%), Gaps = 8/52 (15%)
Frame = -1
Query: 481 GRRSTSA----RTSPNEP-RSARGPSPPCREAAAVQS---RTGAPTWPVRWR 350
GRR + A R SP+ P R R PSPP R + R +PT P R R
Sbjct: 299 GRRQSPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPARRHRSPTPPARQR 350
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,831,845
Number of Sequences: 28952
Number of extensions: 374641
Number of successful extensions: 1304
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1300
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -