Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0677
(604 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 137 7e-33
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 136 2e-32
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 8e-31
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 120 1e-27
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 68 7e-12
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 67 1e-11
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 53 2e-07
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 3e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.058
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.72
SB_37853| Best HMM Match : REJ (HMM E-Value=0.00025) 29 2.2
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.2
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 3.8
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.8
SB_41102| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.7
SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) 28 6.7
SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 27 8.8
SB_24762| Best HMM Match : Astacin (HMM E-Value=0) 27 8.8
SB_47707| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.8
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 137 bits (331), Expect = 7e-33
Identities = 70/119 (58%), Positives = 83/119 (69%)
Frame = +2
Query: 248 PQHTIFDXKRLIGR*FEDATVQADMKHWPFXVVSDGGKPKIXVAYKGEDKTFFPEEVSSM 427
P++TIFD KRLIGR ++DATVQ+DMK WPF ++SD KPKI V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQKMQVPSLA*TFSESFNXPTAAAI 604
VL KMKETAEAYLG+ V +AVITVPAY + + K N PTAAA+
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAAL 181
Score = 127 bits (306), Expect = 8e-30
Identities = 57/63 (90%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 243 MNP 251
+NP
Sbjct: 61 LNP 63
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 136 bits (328), Expect = 2e-32
Identities = 67/119 (56%), Positives = 81/119 (68%)
Frame = +2
Query: 248 PQHTIFDXKRLIGR*FEDATVQADMKHWPFXVVSDGGKPKIXVAYKGEDKTFFPEEVSSM 427
P++T+FD KRLIGR F+D V DM HWPF V+ +G +PKI V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQKMQVPSLA*TFSESFNXPTAAAI 604
VLTKMKETAEAYLG V +AV+TVPAY + + K N PTAAAI
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAAI 181
Score = 132 bits (319), Expect = 2e-31
Identities = 63/68 (92%), Positives = 65/68 (95%), Gaps = 1/68 (1%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 243 MNP-NTQY 263
MNP NT +
Sbjct: 61 MNPENTVF 68
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 130 bits (314), Expect = 8e-31
Identities = 59/63 (93%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 243 MNP 251
MNP
Sbjct: 61 MNP 63
Score = 105 bits (253), Expect = 2e-23
Identities = 61/112 (54%), Positives = 70/112 (62%), Gaps = 25/112 (22%)
Frame = +2
Query: 248 PQHTIFDXKRLIGR*FEDATVQADMKHWPFXVVSDGGKPKIXVAYKGEDKTFFPEEV--- 418
P +TIFD KRLIGR F+D V+ DMKHW F VV++ G+PK+ V YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 419 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
SSMVL KMKETAEAYLG V +AV+TVPAY
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAY 174
Score = 54.8 bits (126), Expect = 5e-08
Identities = 32/64 (50%), Positives = 38/64 (59%)
Frame = +2
Query: 413 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQKMQVPSLA*TFSESFNXPT 592
++SSMVL KMKETAEAYLG V +AV+TVPAY + + K N PT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 593 AAAI 604
AAAI
Sbjct: 271 AAAI 274
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/27 (55%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = +1
Query: 511 NDSQREATKDAG-TISGLNVLRIXQXT 588
NDSQR+ATKDAG ++ G+ R Q T
Sbjct: 176 NDSQRQATKDAGESVRGVCWARSGQMT 202
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 120 bits (288), Expect = 1e-27
Identities = 63/119 (52%), Positives = 78/119 (65%)
Frame = +2
Query: 248 PQHTIFDXKRLIGR*FEDATVQADMKHWPFXVVSDGGKPKIXVAYKGEDKTFFPEEVSSM 427
P++T+FD KRLIGR ++D +VQ D+K +PF VV KP + V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQKMQVPSLA*TFSESFNXPTAAAI 604
VLTKMKETAEAYLGK V +AV+TVPAY + + K T N PTAAAI
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAI 879
Score = 100 bits (239), Expect = 1e-21
Identities = 48/63 (76%), Positives = 55/63 (87%), Gaps = 2/63 (3%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNP-N 254
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 255 TQY 263
T +
Sbjct: 764 TVF 766
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 67.7 bits (158), Expect = 7e-12
Identities = 36/87 (41%), Positives = 51/87 (58%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 QHTIFDXKRLIGR*FEDATVQADMKHWPFXVVS-DGGKPKIXVAYKGEDKTFFPEEVSSM 427
++TI KR IGR F D VQ ++ H P+ VV I V Y G+ + F PE+V +M
Sbjct: 120 KNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAM 179
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
+ T++K TAE L V + VI+VP+Y
Sbjct: 180 LFTRLKTTAEIALKTKVTDCVISVPSY 206
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 236
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 66.9 bits (156), Expect = 1e-11
Identities = 32/68 (47%), Positives = 46/68 (67%), Gaps = 3/68 (4%)
Frame = +3
Query: 69 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 243 MNP-NTQY 263
+NP NT Y
Sbjct: 90 INPKNTIY 97
Score = 62.1 bits (144), Expect = 3e-10
Identities = 43/121 (35%), Positives = 61/121 (50%), Gaps = 2/121 (1%)
Frame = +2
Query: 248 PQHTIFDXKRLIGR*FEDATVQADMKHWPFXVVSDG-GKPKIXVAYKGEDKTFFPEEVSS 424
P++TI+D KR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 425 MVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQKMQVPSLA-*TFSESFNXPTAAA 601
V+ K++ AE L TV AV++VPA +K + ++ SLA T N PTAAA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPA-EFDIKQRNATVKAASLAGITVLRVINEPTAAA 210
Query: 602 I 604
+
Sbjct: 211 L 211
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/47 (51%), Positives = 36/47 (76%)
Frame = +2
Query: 368 IXVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAH 48
Score = 37.9 bits (84), Expect = 0.006
Identities = 16/20 (80%), Positives = 20/20 (100%)
Frame = +1
Query: 511 NDSQREATKDAGTISGLNVL 570
ND+QR+ATKDAGTI+GL+VL
Sbjct: 50 NDAQRQATKDAGTIAGLDVL 69
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 42.3 bits (95), Expect = 3e-04
Identities = 27/61 (44%), Positives = 34/61 (55%), Gaps = 3/61 (4%)
Frame = +3
Query: 132 GKVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNP-NTQYSMXNVSSDVSSKM 302
G IIAND G+R TP+ VAF+DTE+LI +V N N Q S V + + KM
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQVSPKEVINMIYKKM 142
Query: 303 L 305
L
Sbjct: 143 L 143
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 34.7 bits (76), Expect = 0.058
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 380 YKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
Y+ D TF PEE+ M+L + E + +++ V+TVP +
Sbjct: 73 YQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPF 115
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.72
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +3
Query: 141 EIIANDQGNRTTPSYVAFT-DTERL 212
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_37853| Best HMM Match : REJ (HMM E-Value=0.00025)
Length = 2182
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = +1
Query: 481 ECSYHGSRVLNDSQREATKDAG-TISGLNVLRIXQXT 588
ECS+ GS+V+N S+R DAG ++G++ RI T
Sbjct: 678 ECSF-GSKVINSSKRITIYDAGFQVTGISPSRIRMNT 713
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -2
Query: 249 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 151
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 9 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 95
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = -2
Query: 456 SAVSFIFVSTMELTSSGKKVLSSPLYATXILGLPPSLTTXKGQCFMSACTV 304
S F+F ST + +S +KV+ SPL + + P+ G S C V
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNV 1158
>SB_41102| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 178
Score = 27.9 bits (59), Expect = 6.7
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = -1
Query: 571 GERSSQRWYLHLLWLPFES 515
G+RS + WY+++ W P +S
Sbjct: 144 GQRSPREWYVNITWTPSQS 162
>SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14)
Length = 856
Score = 27.9 bits (59), Expect = 6.7
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = -1
Query: 259 CVLGFIATWFLAASPMRRSVSVNAT*DGVVLLPWSLAMISTFPCWKTPTQEYVVPRSIPT 80
C+L I WF P+R S + VV+ S+ ++ F C P + P +IP+
Sbjct: 107 CLLLTIERWFAVVQPLRYQASFSKR--RVVVSLISVYCVACFLCCTLPFEVSFSPENIPS 164
>SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08)
Length = 1011
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/62 (25%), Positives = 27/62 (43%)
Frame = +2
Query: 359 KPKIXVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYSMTLKGKPQK 538
+PK + E+ EE +M ++K LGK V+ + T ++TL + Q
Sbjct: 654 QPKFKKTKEEEEFDKLLEEADAMTQQEVKNVKNIKLGKNVKTQLYTCEPCNITLNSQKQM 713
Query: 539 MQ 544
Q
Sbjct: 714 SQ 715
>SB_24762| Best HMM Match : Astacin (HMM E-Value=0)
Length = 733
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -1
Query: 544 LHLLWLPFESH*VRGNRDNCILHSFAKIS 458
++ LWL F + + D C+LHS A ++
Sbjct: 415 IYTLWLDFSRYYSSASSDICVLHSIAHLA 443
>SB_47707| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 627
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +1
Query: 427 GAYENEG-NCRSLSWQNCAECSYHGSRVLNDSQREAT--KDAGTISGLNVLR 573
GA +N + SL W C + YHG +V+ + A AG S NV+R
Sbjct: 512 GATQNANESLNSLVWARCPKHKYHGYKVIRCAVAAAVCHFHAGASSRENVMR 563
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,885,332
Number of Sequences: 59808
Number of extensions: 452075
Number of successful extensions: 1237
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1226
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1463691625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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