Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0676
(691 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g02680.1 68414.m00218 transcription initiation factor IID (TF... 39 0.003
At2g43010.2 68415.m05338 phytochrome-interacting factor 4 (PIF4)... 31 0.95
At2g43010.1 68415.m05337 phytochrome-interacting factor 4 (PIF4)... 31 0.95
At1g16630.1 68414.m01992 expressed protein 29 2.9
At1g14780.1 68414.m01767 expressed protein 29 3.8
At5g17720.1 68418.m02077 hydrolase, alpha/beta fold family prote... 28 5.1
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati... 28 5.1
At5g46410.1 68418.m05712 NLI interacting factor (NIF) family pro... 28 6.7
At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) fami... 27 8.9
At3g62370.1 68416.m07006 expressed protein 27 8.9
>At1g02680.1 68414.m00218 transcription initiation factor IID
(TFIID) 18 kDa subunit (TAFII-18) family protein similar
to transcription factor TFIID (SP:Q15543) [Homo
sapiens]; contains Pfam PF02269: Transcription
initiation factor IID, 18kD subunit
Length = 126
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +1
Query: 166 TNFQKEISNMMHGFGDNPNPNAATVLLVEKL 258
T FQKE+ +MM+GFGD NP +V LVE +
Sbjct: 29 TLFQKELQHMMYGFGDEQNPLPESVALVEDI 59
>At2g43010.2 68415.m05338 phytochrome-interacting factor 4 (PIF4) /
basic helix-loop-helix protein 9 (bHLH9) / short under
red-light 2 (SRL2) identical to SP|Q8W2F3
Phytochrome-interacting factor 4 (Basic helix-loop-helix
protein 9) (bHLH9) (Short under red-light 2)
{Arabidopsis thaliana}; supporting cDNA
gi|18026965|gb|AF251694.1|AF251694
Length = 428
Score = 30.7 bits (66), Expect = 0.95
Identities = 21/68 (30%), Positives = 37/68 (54%)
Frame = +2
Query: 401 KTGSNRQQITSKRHNISRNYIL*YYTRRREGSDNEQKRTHIDVIKLIDEADEVSQIKFDA 580
++GSNR+ ++ HN+S RRR NE+ + ++I + D+ S I +A
Sbjct: 249 RSGSNRRSRAAEVHNLSE--------RRRRDRINERMKALQELIPHCSKTDKAS-ILDEA 299
Query: 581 IDYLREVR 604
IDYL+ ++
Sbjct: 300 IDYLKSLQ 307
>At2g43010.1 68415.m05337 phytochrome-interacting factor 4 (PIF4) /
basic helix-loop-helix protein 9 (bHLH9) / short under
red-light 2 (SRL2) identical to SP|Q8W2F3
Phytochrome-interacting factor 4 (Basic helix-loop-helix
protein 9) (bHLH9) (Short under red-light 2)
{Arabidopsis thaliana}; supporting cDNA
gi|18026965|gb|AF251694.1|AF251694
Length = 430
Score = 30.7 bits (66), Expect = 0.95
Identities = 21/68 (30%), Positives = 37/68 (54%)
Frame = +2
Query: 401 KTGSNRQQITSKRHNISRNYIL*YYTRRREGSDNEQKRTHIDVIKLIDEADEVSQIKFDA 580
++GSNR+ ++ HN+S RRR NE+ + ++I + D+ S I +A
Sbjct: 249 RSGSNRRSRAAEVHNLSE--------RRRRDRINERMKALQELIPHCSKTDKAS-ILDEA 299
Query: 581 IDYLREVR 604
IDYL+ ++
Sbjct: 300 IDYLKSLQ 307
>At1g16630.1 68414.m01992 expressed protein
Length = 845
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/72 (29%), Positives = 38/72 (52%)
Frame = +2
Query: 410 SNRQQITSKRHNISRNYIL*YYTRRREGSDNEQKRTHIDVIKLIDEADEVSQIKFDAIDY 589
S R++I S ++ +SR++ ++ + + S + DVI +I E +V QI D
Sbjct: 111 SPRKKILSDKNEVSRSFDKSHHQVQVKSSVSFS-----DVISIIGEDKDVDQICIDETKQ 165
Query: 590 LREVRASELTES 625
LRE + ++T S
Sbjct: 166 LREEESHDITVS 177
>At1g14780.1 68414.m01767 expressed protein
Length = 627
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/21 (57%), Positives = 16/21 (76%), Gaps = 3/21 (14%)
Frame = +1
Query: 379 NVCTITSK---NWIKSTTNHK 432
+VCT+ K NWIK+T+NHK
Sbjct: 454 HVCTVPVKYDPNWIKTTSNHK 474
>At5g17720.1 68418.m02077 hydrolase, alpha/beta fold family protein
low similarity to SP|P27747 Dihydrolipoamide
acetyltransferase component of acetoin cleaving system
(EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes
eutrophus}, SP|P24640 Lipase 3 precursor (EC 3.1.1.3)
(Triacylglycerol lipase) {Moraxella sp}; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 443
Score = 28.3 bits (60), Expect = 5.1
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +1
Query: 139 YTMETCGEGTNFQKEISNMMHGFGDNPNPNAATVLLVE 252
Y ET EGTN++ +++ GFGD+P P A+ L E
Sbjct: 183 YLPETT-EGTNYRFFAIDLL-GFGDSPKPRASQYSLKE 218
>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 235
Score = 28.3 bits (60), Expect = 5.1
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +3
Query: 252 KIVLQQLRSILQEGIFNSNLRGSKTITNYDIIYTMRNNTVKLKRLHDYQQKLDQIDNKSQ 431
K + Q +R I QE +N ++I + K K DY +KL Q+D + +
Sbjct: 8 KQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRKLKQVDIRKR 67
Query: 432 VNVTT 446
++ +T
Sbjct: 68 IDYST 72
>At5g46410.1 68418.m05712 NLI interacting factor (NIF) family
protein contains Pfam profile PF03031: NLI interacting
factor
Length = 453
Score = 27.9 bits (59), Expect = 6.7
Identities = 17/73 (23%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +3
Query: 282 LQEGIFNSNLRGSKTIT-NYDIIYTMRNNTVKLKRLHDYQQKLDQIDNKSQVNVTTSAET 458
L E + +S L ++ + + M+ NTV +++ + L+++ V + T++ +
Sbjct: 287 LDETLVHSTLESCNVADFSFRVFFNMQENTVYVRQRPHLYRFLERVGELFHVVIFTASHS 346
Query: 459 IYSNIILEDEKDP 497
IY++ +L D DP
Sbjct: 347 IYASQLL-DILDP 358
>At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) family
protein similar to TAR RNA loop binding protein [Homo
sapiens] GI:1184692; contains Pfam profile PF00588: SpoU
rRNA Methylase (RNA methyltransferase, TrmH) family
Length = 1850
Score = 27.5 bits (58), Expect = 8.9
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +3
Query: 405 LDQIDNKSQVNVTTSAETIYSNIILEDEK 491
L D+K+QVN+ + +++N+IL EK
Sbjct: 1439 LKNYDSKAQVNLRKTKMIVFANVILHAEK 1467
>At3g62370.1 68416.m07006 expressed protein
Length = 361
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +1
Query: 106 IFLRIGDKMTYYTMETCGEGTN 171
+ +IGD+ TY+ M C EGT+
Sbjct: 118 LMFQIGDQATYHNMGGCKEGTD 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,096,455
Number of Sequences: 28952
Number of extensions: 243776
Number of successful extensions: 643
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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