Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e96h0625
(649 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 52 2e-07
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 52 4e-07
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 51 6e-07
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 51 8e-07
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 50 1e-06
At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma m... 33 0.12
At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma m... 33 0.12
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 32 0.29
At3g57330.1 68416.m06381 calcium-transporting ATPase, plasma mem... 29 2.7
At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 29 3.5
At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacu... 29 3.5
At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacu... 29 3.5
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 4.7
At3g59010.1 68416.m06577 pectinesterase family protein contains ... 28 6.1
At4g37390.1 68417.m05294 auxin-responsive GH3 family protein sim... 27 8.1
At1g32160.1 68414.m03956 expressed protein 27 8.1
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 52.4 bits (120), Expect = 2e-07
Identities = 29/72 (40%), Positives = 39/72 (54%), Gaps = 7/72 (9%)
Frame = +2
Query: 59 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 217
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60
Query: 218 SLLTSGGVTIAG 253
L G+ +AG
Sbjct: 61 GHLAPTGMFLAG 72
Score = 47.2 bits (107), Expect = 9e-06
Identities = 22/43 (51%), Positives = 28/43 (65%)
Frame = +3
Query: 303 GKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 431
G G+ KT Q++V LYEEP+ P Q VVE+LG+YLI G
Sbjct: 91 GAGGITIKKTGQSMVFGLYEEPVTPGQCNMVVERLGDYLIEQG 133
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 51.6 bits (118), Expect = 4e-07
Identities = 29/73 (39%), Positives = 40/73 (54%), Gaps = 7/73 (9%)
Frame = +2
Query: 59 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 217
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60
Query: 218 SLLTSGGVTIAGS 256
L G+ +AG+
Sbjct: 61 GHLAPTGLFMAGA 73
Score = 44.8 bits (101), Expect = 5e-05
Identities = 20/43 (46%), Positives = 27/43 (62%)
Frame = +3
Query: 303 GKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 431
G G+ KT Q+ V +YEEP+ P Q VVE+LG+YL+ G
Sbjct: 91 GAGGITIKKTGQSCVFGIYEEPVTPGQCNMVVERLGDYLLEQG 133
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 51.2 bits (117), Expect = 6e-07
Identities = 28/70 (40%), Positives = 38/70 (54%), Gaps = 4/70 (5%)
Frame = +2
Query: 59 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 226
MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97
Query: 227 TSGGVTIAGS 256
G+ + G+
Sbjct: 98 APTGLFLGGN 107
Score = 44.8 bits (101), Expect = 5e-05
Identities = 22/43 (51%), Positives = 26/43 (60%)
Frame = +3
Query: 303 GKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 431
G GV KT A+V +Y+EP+ P Q VVE LGEYLI G
Sbjct: 125 GAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVENLGEYLIESG 167
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 50.8 bits (116), Expect = 8e-07
Identities = 29/69 (42%), Positives = 37/69 (53%), Gaps = 4/69 (5%)
Frame = +2
Query: 59 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 226
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60
Query: 227 TSGGVTIAG 253
G+ + G
Sbjct: 61 APTGLFLGG 69
Score = 43.2 bits (97), Expect = 2e-04
Identities = 21/43 (48%), Positives = 27/43 (62%)
Frame = +3
Query: 303 GKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLITCG 431
G GV KT QA+V +Y+EP+ Q VVE+LG+YLI G
Sbjct: 88 GPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVERLGDYLIESG 130
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 50.0 bits (114), Expect = 1e-06
Identities = 28/69 (40%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
Frame = +2
Query: 59 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 226
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60
Query: 227 TSGGVTIAG 253
G+ + G
Sbjct: 61 APTGLFLGG 69
Score = 41.1 bits (92), Expect = 6e-04
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +3
Query: 303 GKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLI 422
G GV KT QA+V Y+EP+ Q VVE+LG+YLI
Sbjct: 88 GPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVERLGDYLI 127
>At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 946
Score = 33.5 bits (73), Expect = 0.12
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = +2
Query: 152 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAGSGTSTSVAQTISSA 295
+ GFEI DE+ IV G + + L GG STS+A IS++
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTS 139
>At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 1020
Score = 33.5 bits (73), Expect = 0.12
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = +2
Query: 152 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAGSGTSTSVAQTISSA 295
+ GFEI DE+ IV G + + L GG STS+A IS++
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTS 139
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 32.3 bits (70), Expect = 0.29
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +2
Query: 152 KSEGFEISKDEVAKIVAGFENESLLTSGGVTIAGSGTSTSVAQTISSA 295
K GF I DE+A +V + +SL GGV S S+++ I S+
Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGVEELAKKVSVSLSEGIRSS 136
>At3g57330.1 68416.m06381 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA11)
identical to SP|Q9M2L4|ACAB_ARATH Potential
calcium-transporting ATPase 11, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 11) {Arabidopsis
thaliana}; strong similarity to calmodulin-stimulated
calcium-ATPase [Brassica oleracea] GI:1805654
Length = 1025
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = -3
Query: 389 CGLLRLDGFFIERNDHSLLCLHAMHADLAKFARMIWSVPL 270
CG+L G IE +D L H M A L K M S+PL
Sbjct: 686 CGILTAGGVAIEGSDFRNLPPHEMRAILPKIQVMARSLPL 725
>At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar
to sodium/calcium exchanger protein [Mus musculus]
gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 559
Score = 28.7 bits (61), Expect = 3.5
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -2
Query: 285 MVCATEVDVPLPAIVTPPLVSSDSFSKPATI 193
++ A + + LP I+T P+VS D +SKP +
Sbjct: 307 VIWAITLPLNLPRILTIPVVSEDKWSKPLAV 337
>At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 28.7 bits (61), Expect = 3.5
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 515 IQMQLLISPWKKYYLIVLLDHGELRPLITT 426
IQ L PW KY + ++ D G +PL T+
Sbjct: 754 IQYNLCNEPWIKYSISIVADKGLKKPLFTS 783
>At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 28.7 bits (61), Expect = 3.5
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 515 IQMQLLISPWKKYYLIVLLDHGELRPLITT 426
IQ L PW KY + ++ D G +PL T+
Sbjct: 754 IQYNLCNEPWIKYSISIVADKGLKKPLFTS 783
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +2
Query: 152 KSEGFEISKDEVAKIVAGFENESLLTSGGV 241
K+ GF+I DE+ IV G + + L GGV
Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119
>At3g59010.1 68416.m06577 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 529
Score = 27.9 bits (59), Expect = 6.1
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = +2
Query: 215 ESLLTSGGVTIAGSGTSTSVAQTISSARTWQGRRALH 325
E L V GSGT SVA+ ++S GR +H
Sbjct: 223 EELRPHAVVAADGSGTHMSVAEALASLEKGSGRSVIH 259
>At4g37390.1 68417.m05294 auxin-responsive GH3 family protein
similar to auxin-responsive GH3 product [Glycine max]
GI:18591; contains Pfam profile PF03321: GH3
auxin-responsive promoter
Length = 603
Score = 27.5 bits (58), Expect = 8.1
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = +2
Query: 35 YF*LNLIKMSWQDYVDKQLMASRCVTKAAIAGHDGNVWAKSEGFEISKDEVAKIVAGFEN 214
YF +NL M V +M + + HDG+ A++ E S E+A + G E
Sbjct: 344 YFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEY 403
Query: 215 ESLLTS 232
E ++T+
Sbjct: 404 ELVITT 409
>At1g32160.1 68414.m03956 expressed protein
Length = 406
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -2
Query: 561 IEYQNNERSSYHFIEYTNAVTYFPVEKILSYSTLRSRRAST 439
I+ +E SSY Y VTY + + Y+T+RS+ A T
Sbjct: 301 IQRNEDESSSYGLRSY---VTYLDSDTLQRYATIRSKEAMT 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,542,025
Number of Sequences: 28952
Number of extensions: 299753
Number of successful extensions: 755
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 750
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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