BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0877 (469 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 52 2e-07 At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 52 3e-07 At3g29375.1 68416.m03690 XH domain-containing protein contains P... 31 0.39 At5g06839.1 68418.m00773 bZIP family transcription factor contai... 29 1.2 At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 29 1.2 At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 27 4.8 At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 4.8 At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 27 4.8 At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 27 4.8 At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 27 6.3 At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase fa... 27 6.3 At1g19130.1 68414.m02379 expressed protein 27 6.3 At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1) (... 27 8.4 >At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 52.0 bits (119), Expect = 2e-07 Identities = 31/65 (47%), Positives = 38/65 (58%) Frame = +2 Query: 59 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 238 MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57 Query: 239 DYRRY 253 ++ RY Sbjct: 58 NFTRY 62 >At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|P47823 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus cuniculus}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 51.6 bits (118), Expect = 3e-07 Identities = 32/65 (49%), Positives = 38/65 (58%) Frame = +2 Query: 59 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 238 MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57 Query: 239 DYRRY 253 ++ RY Sbjct: 58 NFSRY 62 >At3g29375.1 68416.m03690 XH domain-containing protein contains Pfam profile: PF03469: XH domain Length = 335 Score = 31.1 bits (67), Expect = 0.39 Identities = 22/66 (33%), Positives = 30/66 (45%) Frame = -3 Query: 410 SFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSHSVGSRVL 231 S P +F V S L+ G SPSI GSS S+++ T+H+ S Sbjct: 46 SSATPSFEFGTVPSSPSTAPSTSLLFGSSPSI-FGATGSSPSSLPSSASTTTHATSSVTT 104 Query: 230 IRPSLG 213 +PSLG Sbjct: 105 TQPSLG 110 >At5g06839.1 68418.m00773 bZIP family transcription factor contains Pfam profile: PF00170 bZIP transcription factor Length = 417 Score = 29.5 bits (63), Expect = 1.2 Identities = 17/63 (26%), Positives = 25/63 (39%), Gaps = 3/63 (4%) Frame = -3 Query: 299 GSSRPPAMSTSNMTSHSVGSRVLIRPSLGTCRL--RRDRH-RPAPDPVPERRGNRSGHTS 129 G PP+ + S + H + +RP T + + H P P + GNR G S Sbjct: 47 GHHDPPSTAPSPLHHHHTTQNLAMRPPTSTLNIFPSQPMHIEPPPSSTHNKEGNRKGLAS 106 Query: 128 LSH 120 H Sbjct: 107 SDH 109 >At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80 subunit, putative contains 5 WD-40 repeats (PF00400); similar to katanin p80 subunit (GI:3005601) [Strongylocentrotus purpuratus] Length = 1180 Score = 29.5 bits (63), Expect = 1.2 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 2/43 (4%) Frame = -3 Query: 365 SLPEKMQVLVLGDSPSI--DTEPPGSSRPPAMSTSNMTSHSVG 243 S P ++ +VLG S+ DT P S+RP S +N+TS G Sbjct: 688 STPVRVMPVVLGRDTSMATDTPPVTSTRPDRTSATNLTSDVSG 730 >At5g53870.1 68418.m06701 plastocyanin-like domain-containing protein contains similarity to SP|Q02917 Early nodulin 55-2 precursor {Glycine max}; PF02298: Plastocyanin-like domain Length = 370 Score = 27.5 bits (58), Expect = 4.8 Identities = 20/47 (42%), Positives = 26/47 (55%), Gaps = 4/47 (8%) Frame = -3 Query: 377 VSISSLPEKMQVLVLGDSPSIDTEPPGSSRP----PAMSTSNMTSHS 249 V+ +S P K Q SP+ +PP SS P PA+S S+ TSHS Sbjct: 160 VAPASAPSKSQPPRSSVSPA---QPPKSSSPISHTPALSPSHATSHS 203 >At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative similar to alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil GI:21670851; contains Pfam profile PF00128: Alpha amylase, catalytic domain Length = 423 Score = 27.5 bits (58), Expect = 4.8 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = +2 Query: 119 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 214 D K YD N R L Q +E AGG + A+ + Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269 >At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 662 Score = 27.5 bits (58), Expect = 4.8 Identities = 14/51 (27%), Positives = 27/51 (52%) Frame = -3 Query: 434 SKYLYRRISFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPP 282 S++ ++ + P S FR +S P +++ LV +PS +PP ++ P Sbjct: 326 SEWRSLKVQWDEPSSVFRPERVS--PWELEPLVANSTPSSQPQPPQRNKRP 374 >At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 665 Score = 27.5 bits (58), Expect = 4.8 Identities = 14/51 (27%), Positives = 27/51 (52%) Frame = -3 Query: 434 SKYLYRRISFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPP 282 S++ ++ + P S FR +S P +++ LV +PS +PP ++ P Sbjct: 326 SEWRSLKVQWDEPSSVFRPERVS--PWELEPLVANSTPSSQPQPPQRNKRP 374 >At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast, putative similar to SP|Q03460 Glutamate synthase [NADH], chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT) {Medicago sativa} Length = 2208 Score = 27.1 bits (57), Expect = 6.3 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -2 Query: 381 HGVHILIAGEDAGVGLGRF 325 H H+LIAG D G G R+ Sbjct: 1179 HADHVLIAGHDGGTGASRW 1197 >At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase family protein similar to SP|P31384 Glucose-repressible alcohol dehydrogenase transcriptional effector (Carbon catabolite repressor protein 4) {Saccharomyces cerevisiae}; contains Pfam profile PF03372: Endonuclease/Exonuclease/phosphatase family Length = 602 Score = 27.1 bits (57), Expect = 6.3 Identities = 21/68 (30%), Positives = 28/68 (41%) Frame = -3 Query: 212 TCRLRRDRHRPAPDPVPERRGNRSGHTSLSHLFFWS*SVDPIILVFLPSDSYNILILTGY 33 +C + R PAP P P R + SG HL + P+ + SYNIL T Sbjct: 205 SCTILTSRVIPAPSPSPRRLISISGTDVTGHL---DSNGRPLSMGTFTVLSYNILSDTYA 261 Query: 32 VGAFYXFC 9 Y +C Sbjct: 262 SSDIYSYC 269 >At1g19130.1 68414.m02379 expressed protein Length = 187 Score = 27.1 bits (57), Expect = 6.3 Identities = 19/70 (27%), Positives = 32/70 (45%) Frame = +1 Query: 235 TRLPTLCEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSGNEDMDTMRNFEQGFVKLM 414 ++LP +V V + LLP GSVS P T F E + +R+ ++ Sbjct: 40 SQLPPTFKVDRAVSTSIYFLLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTS 99 Query: 415 RRYKYLEKMF 444 ++L++MF Sbjct: 100 GLVRFLQRMF 109 >At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1) (PK6) identical to serine/threonine-protein kinase AtPK1/AtPK6 (ribosomal-protein S6 kinase ATPK6) [Arabidopsis thaliana] SWISS-PROT:P42818 Length = 465 Score = 26.6 bits (56), Expect = 8.4 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +1 Query: 316 MDGESPKTNTCIFSGNEDMDTMRNFEQGFVKL 411 ++GES K N FSGN+D D + E VK+ Sbjct: 95 LEGESLKENDD-FSGNDDSDNEKALEGDLVKV 125 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,417,762 Number of Sequences: 28952 Number of extensions: 231077 Number of successful extensions: 842 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 805 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 842 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 791932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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