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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0877
         (469 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    52   2e-07
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    52   3e-07
At3g29375.1 68416.m03690 XH domain-containing protein contains P...    31   0.39 
At5g06839.1 68418.m00773 bZIP family transcription factor contai...    29   1.2  
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p...    29   1.2  
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro...    27   4.8  
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g...    27   4.8  
At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica...    27   4.8  
At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica...    27   4.8  
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,...    27   6.3  
At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase fa...    27   6.3  
At1g19130.1 68414.m02379 expressed protein                             27   6.3  
At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1) (...    27   8.4  

>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 31/65 (47%), Positives = 38/65 (58%)
 Frame = +2

Query: 59  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 238
           MS K EKP L G RIKTRKR+     DP  F DA+VQ      GDL+   K ++S  S L
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57

Query: 239 DYRRY 253
           ++ RY
Sbjct: 58  NFTRY 62


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 51.6 bits (118), Expect = 3e-07
 Identities = 32/65 (49%), Positives = 38/65 (58%)
 Frame = +2

Query: 59  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 238
           MS K EKP L G RIKTRKR+     DP  F DALVQ      GDL+   + L+S  S L
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57

Query: 239 DYRRY 253
           ++ RY
Sbjct: 58  NFSRY 62


>At3g29375.1 68416.m03690 XH domain-containing protein contains Pfam
           profile: PF03469: XH domain
          Length = 335

 Score = 31.1 bits (67), Expect = 0.39
 Identities = 22/66 (33%), Positives = 30/66 (45%)
 Frame = -3

Query: 410 SFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSHSVGSRVL 231
           S   P  +F  V  S        L+ G SPSI     GSS     S+++ T+H+  S   
Sbjct: 46  SSATPSFEFGTVPSSPSTAPSTSLLFGSSPSI-FGATGSSPSSLPSSASTTTHATSSVTT 104

Query: 230 IRPSLG 213
            +PSLG
Sbjct: 105 TQPSLG 110


>At5g06839.1 68418.m00773 bZIP family transcription factor contains
           Pfam profile: PF00170 bZIP transcription factor
          Length = 417

 Score = 29.5 bits (63), Expect = 1.2
 Identities = 17/63 (26%), Positives = 25/63 (39%), Gaps = 3/63 (4%)
 Frame = -3

Query: 299 GSSRPPAMSTSNMTSHSVGSRVLIRPSLGTCRL--RRDRH-RPAPDPVPERRGNRSGHTS 129
           G   PP+ + S +  H     + +RP   T  +   +  H  P P     + GNR G  S
Sbjct: 47  GHHDPPSTAPSPLHHHHTTQNLAMRPPTSTLNIFPSQPMHIEPPPSSTHNKEGNRKGLAS 106

Query: 128 LSH 120
             H
Sbjct: 107 SDH 109


>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
           subunit, putative contains 5 WD-40 repeats (PF00400);
           similar to katanin p80 subunit (GI:3005601)
           [Strongylocentrotus purpuratus]
          Length = 1180

 Score = 29.5 bits (63), Expect = 1.2
 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
 Frame = -3

Query: 365 SLPEKMQVLVLGDSPSI--DTEPPGSSRPPAMSTSNMTSHSVG 243
           S P ++  +VLG   S+  DT P  S+RP   S +N+TS   G
Sbjct: 688 STPVRVMPVVLGRDTSMATDTPPVTSTRPDRTSATNLTSDVSG 730


>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
           protein contains similarity to SP|Q02917 Early nodulin
           55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
           domain
          Length = 370

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 20/47 (42%), Positives = 26/47 (55%), Gaps = 4/47 (8%)
 Frame = -3

Query: 377 VSISSLPEKMQVLVLGDSPSIDTEPPGSSRP----PAMSTSNMTSHS 249
           V+ +S P K Q      SP+   +PP SS P    PA+S S+ TSHS
Sbjct: 160 VAPASAPSKSQPPRSSVSPA---QPPKSSSPISHTPALSPSHATSHS 203


>At4g25000.1 68417.m03587 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
           GI:21670851; contains Pfam profile PF00128: Alpha
           amylase, catalytic domain
          Length = 423

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 13/32 (40%), Positives = 17/32 (53%)
 Frame = +2

Query: 119 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 214
           D K  YD N  R  L Q +E AGG +  A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269


>At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical
           to auxin response factor 1 GI:2245378 from [Arabidopsis
           thaliana]
          Length = 662

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 14/51 (27%), Positives = 27/51 (52%)
 Frame = -3

Query: 434 SKYLYRRISFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPP 282
           S++   ++ +  P S FR   +S  P +++ LV   +PS   +PP  ++ P
Sbjct: 326 SEWRSLKVQWDEPSSVFRPERVS--PWELEPLVANSTPSSQPQPPQRNKRP 374


>At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical
           to auxin response factor 1 GI:2245378 from [Arabidopsis
           thaliana]
          Length = 665

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 14/51 (27%), Positives = 27/51 (52%)
 Frame = -3

Query: 434 SKYLYRRISFTNPCSKFRMVSISSLPEKMQVLVLGDSPSIDTEPPGSSRPP 282
           S++   ++ +  P S FR   +S  P +++ LV   +PS   +PP  ++ P
Sbjct: 326 SEWRSLKVQWDEPSSVFRPERVS--PWELEPLVANSTPSSQPQPPQRNKRP 374


>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
            putative similar to SP|Q03460 Glutamate synthase [NADH],
            chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
            {Medicago sativa}
          Length = 2208

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = -2

Query: 381  HGVHILIAGEDAGVGLGRF 325
            H  H+LIAG D G G  R+
Sbjct: 1179 HADHVLIAGHDGGTGASRW 1197


>At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase
           family protein similar to SP|P31384 Glucose-repressible
           alcohol dehydrogenase transcriptional effector (Carbon
           catabolite repressor protein 4) {Saccharomyces
           cerevisiae}; contains Pfam profile PF03372:
           Endonuclease/Exonuclease/phosphatase family
          Length = 602

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 21/68 (30%), Positives = 28/68 (41%)
 Frame = -3

Query: 212 TCRLRRDRHRPAPDPVPERRGNRSGHTSLSHLFFWS*SVDPIILVFLPSDSYNILILTGY 33
           +C +   R  PAP P P R  + SG     HL     +  P+ +      SYNIL  T  
Sbjct: 205 SCTILTSRVIPAPSPSPRRLISISGTDVTGHL---DSNGRPLSMGTFTVLSYNILSDTYA 261

Query: 32  VGAFYXFC 9
               Y +C
Sbjct: 262 SSDIYSYC 269


>At1g19130.1 68414.m02379 expressed protein
          Length = 187

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 19/70 (27%), Positives = 32/70 (45%)
 Frame = +1

Query: 235 TRLPTLCEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSGNEDMDTMRNFEQGFVKLM 414
           ++LP   +V   V  +   LLP GSVS     P   T  F   E +  +R+     ++  
Sbjct: 40  SQLPPTFKVDRAVSTSIYFLLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTS 99

Query: 415 RRYKYLEKMF 444
              ++L++MF
Sbjct: 100 GLVRFLQRMF 109


>At3g08730.1 68416.m01015 serine/threonine protein kinase (PK1)
           (PK6) identical to serine/threonine-protein kinase
           AtPK1/AtPK6 (ribosomal-protein S6 kinase ATPK6)
           [Arabidopsis thaliana] SWISS-PROT:P42818
          Length = 465

 Score = 26.6 bits (56), Expect = 8.4
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +1

Query: 316 MDGESPKTNTCIFSGNEDMDTMRNFEQGFVKL 411
           ++GES K N   FSGN+D D  +  E   VK+
Sbjct: 95  LEGESLKENDD-FSGNDDSDNEKALEGDLVKV 125


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,417,762
Number of Sequences: 28952
Number of extensions: 231077
Number of successful extensions: 842
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 842
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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