Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0840
(745 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 63 2e-10
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 59 4e-09
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 56 2e-08
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 55 6e-08
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 54 1e-07
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 53 2e-07
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 52 5e-07
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 48 5e-06
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 48 7e-06
At4g32290.1 68417.m04594 expressed protein contains Pfam profile... 30 1.4
At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containi... 30 1.4
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ... 30 1.9
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 29 3.3
At3g55950.1 68416.m06217 protein kinase family protein contains ... 29 4.3
At3g10630.1 68416.m01278 glycosyl transferase family 1 protein c... 28 5.7
At2g20060.1 68415.m02344 ribosomal protein L4 family protein con... 28 5.7
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative... 28 5.7
At4g28050.1 68417.m04024 senescence-associated protein, putative... 28 7.5
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 7.5
At4g30430.1 68417.m04322 senescence-associated family protein si... 27 9.9
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 63.3 bits (147), Expect = 2e-10
Identities = 33/93 (35%), Positives = 47/93 (50%)
Frame = +1
Query: 277 YLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDA 456
+ F + A+W T +F Y GLGIT HR AH+S+K L LL +A Q
Sbjct: 59 FYFTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGD 114
Query: 457 VVDWARDHRMHHKYSETVRTPTTQPEDSSFPTL 555
+DW HR HH+++++ R P + E F L
Sbjct: 115 PIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 34.7 bits (76), Expect = 0.065
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 656
DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 134 DPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 58.8 bits (136), Expect = 4e-09
Identities = 29/89 (32%), Positives = 41/89 (46%)
Frame = +1
Query: 289 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 468
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 469 ARDHRMHHKYSETVRTPTTQPEDSSFPTL 555
HR HH+++++ R P + E F L
Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
Score = 33.1 bits (72), Expect = 0.20
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQIL 671
DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y IL
Sbjct: 132 DPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYHIL 185
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 56.0 bits (129), Expect = 2e-08
Identities = 30/93 (32%), Positives = 41/93 (44%)
Frame = +1
Query: 289 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 468
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 469 ARDHRMHHKYSETVRTPTTQPEDSSFPTLAGCC 567
HR HH+++++ R P + E F + C
Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
Score = 33.5 bits (73), Expect = 0.15
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 626
DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 126 DPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 54.8 bits (126), Expect = 6e-08
Identities = 28/78 (35%), Positives = 39/78 (50%), Gaps = 1/78 (1%)
Frame = +1
Query: 289 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 465
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 466 WARDHRMHHKYSETVRTP 519
W HR HH++ ++ R P
Sbjct: 181 WVSTHRYHHQFCDSDRDP 198
Score = 40.3 bits (90), Expect = 0.001
Identities = 22/57 (38%), Positives = 30/57 (52%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQILMPLA 683
DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL PLA
Sbjct: 197 DPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-ILHPLA 251
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 53.6 bits (123), Expect = 1e-07
Identities = 31/98 (31%), Positives = 43/98 (43%), Gaps = 1/98 (1%)
Frame = +1
Query: 229 KCDSVLYSAVGGVYGGYLFL-FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARL 405
+ D SAVG V+ L F W F + L + + L IT HR HKS+K
Sbjct: 30 RIDIARASAVGAVHLLCLLAPFNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPK 89
Query: 406 PLRILLTIFNTIAFQDAVVDWARDHRMHHKYSETVRTP 519
L A Q +DW HR HH+++++ R P
Sbjct: 90 WLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDP 127
Score = 33.5 bits (73), Expect = 0.15
Identities = 16/45 (35%), Positives = 23/45 (51%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 647
DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 126 DPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 53.2 bits (122), Expect = 2e-07
Identities = 29/96 (30%), Positives = 47/96 (48%)
Frame = +1
Query: 271 GGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQ 450
G L +A W +F +++ +G+ IT HR +H+S+ L L +AFQ
Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQ 98
Query: 451 DAVVDWARDHRMHHKYSETVRTPTTQPEDSSFPTLA 558
++W +HR HHK+ ET R P + + F +A
Sbjct: 99 GDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 134
Score = 37.9 bits (84), Expect = 0.007
Identities = 19/60 (31%), Positives = 32/60 (53%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQILMPLACFI 692
DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++ + M F+
Sbjct: 120 DPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFL 178
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 51.6 bits (118), Expect = 5e-07
Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 1/98 (1%)
Frame = +1
Query: 229 KCDSVLYSAVGGVYGGYLFL-FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARL 405
+ D + SAVG V+ L F W FA + + + L IT HR H+S+K
Sbjct: 30 RVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPK 89
Query: 406 PLRILLTIFNTIAFQDAVVDWARDHRMHHKYSETVRTP 519
L A Q +DW HR HH+++++ R P
Sbjct: 90 WLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDP 127
Score = 33.5 bits (73), Expect = 0.15
Identities = 16/45 (35%), Positives = 23/45 (51%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 647
DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 126 DPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 48.4 bits (110), Expect = 5e-06
Identities = 25/86 (29%), Positives = 38/86 (44%)
Frame = +1
Query: 289 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 468
F W F + L+ + L IT HR +H+S+K L A Q +DW
Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111
Query: 469 ARDHRMHHKYSETVRTPTTQPEDSSF 546
HR HH+++++ R P + E F
Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137
Score = 31.5 bits (68), Expect = 0.61
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 656
DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 127 DPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 48.0 bits (109), Expect = 7e-06
Identities = 24/77 (31%), Positives = 35/77 (45%)
Frame = +1
Query: 289 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 468
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 469 ARDHRMHHKYSETVRTP 519
HR HH+++++ R P
Sbjct: 111 VSIHRFHHQFTDSDRDP 127
Score = 35.9 bits (79), Expect = 0.028
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +3
Query: 513 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQILMPLACFI 692
DPH+ GF+FSH+ W+ + K G +V DL+ RF KK +++ + F
Sbjct: 126 DPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK--TLVLHILAFW 182
Query: 693 MPTYVPTLWG 722
Y LWG
Sbjct: 183 TLIY---LWG 189
>At4g32290.1 68417.m04594 expressed protein contains Pfam profile
PF03267: Arabidopsis protein of unknown function, DUF266
Length = 384
Score = 30.3 bits (65), Expect = 1.4
Identities = 15/30 (50%), Positives = 21/30 (70%), Gaps = 1/30 (3%)
Frame = +1
Query: 469 ARDHRMHHKYSET-VRTPTTQPEDSSFPTL 555
ARD R+ K+++T VR + PE+S FPTL
Sbjct: 256 ARDRRIWVKFNQTCVREDSCYPEESYFPTL 285
>At3g16890.1 68416.m02159 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 659
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +1
Query: 82 VHCFSENASPKETGGFVGIVRSRRQ*P-ARRCTAPCTSLSREETLEDCVEKCDSVLYSAV 258
++C S N+ KETG F+ + R P + A + L + L + D + V
Sbjct: 327 LYCLSNNSMAKETGQFLRKIGERGYIPDSSTFNAAMSCLLKGHDLVETCRIFDGFVSRGV 386
Query: 259 GGVYGGYLFLFKAMWRTSIFA 321
+ GYL L +A+ F+
Sbjct: 387 KPGFNGYLVLVQALLNAQRFS 407
>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
identical to metal transporter Nramp4 [Arabidopsis
thaliana] gi|6468014|gb|AAF13279; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 512
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 262 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 369
G GY ++ MW T+I + L + LG+ G H
Sbjct: 79 GAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRH 114
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 262 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 369
G GY L+ MW T++ + L + LG+ G H
Sbjct: 83 GAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRH 118
>At3g55950.1 68416.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
cytokinin-regulated kinase 1 [Nicotiana tabacum]
gi|10998537|gb|AAG25966
Length = 814
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/41 (36%), Positives = 19/41 (46%)
Frame = +2
Query: 164 PEDAPPHVPPSAEKRPWKIVWRNVILFFILLSVVFMEGTCS 286
P PP PS P K + R ++ F I+ SV G CS
Sbjct: 372 PPPPPPPPSPSTSSPPSKALTRGLLAFAIVGSVGAFAGICS 412
>At3g10630.1 68416.m01278 glycosyl transferase family 1 protein
contains Pfam glycosyl transferase, group 1 family
protein domain PF00534; C-terminal portion similar to
mannosyltransferase GB:BAA28328 [Escherichia coli]
Length = 487
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/31 (48%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = +2
Query: 188 PPSAEKRPWK---IVWRNVILFFILLSVVFM 271
PP KRPWK IV+ + IL F+LLS+ +
Sbjct: 4 PPDRSKRPWKFSTIVYSSSIL-FLLLSIFLL 33
>At2g20060.1 68415.m02344 ribosomal protein L4 family protein
contains Pfam profile PF00573: ribosomal protein L4/L1
family
Length = 300
Score = 28.3 bits (60), Expect = 5.7
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = -1
Query: 625 RRSFTSTVWPLALISGCFLNNNQPMWEKKNPRVALWGSARSLNICGACDDHGPNPLRHPE 446
R+ T + L+ +SG +P W +K A G+ R G C HGP P H
Sbjct: 131 RQQGTHSTKTLSEVSG---TGRKP-WNQKGTGRARHGTLRGPQFRGGCVMHGPKPRSHAI 186
Query: 445 K 443
K
Sbjct: 187 K 187
>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
(NRAMP2) similar to metal transporter Nramp3
[Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
the natural resistance-associated macrophage protein
(NRAMP) metal transporter family, PMID:11500563
Length = 530
Score = 28.3 bits (60), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +1
Query: 262 GVYGGYLFLFKAMWRTSIFAIFLYLCSGLGITAGAH 369
G GY L+ MW T++ + L + +G+ G H
Sbjct: 95 GAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130
>At4g28050.1 68417.m04024 senescence-associated protein, putative
similar to senescence-associated protein 5 [Hemerocallis
hybrid cultivar] gi|3551954|gb|AAC34855
Length = 263
Score = 27.9 bits (59), Expect = 7.5
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 203 KRPWKIVWRNVILFFILLSVVFMEGTCSF 289
K WK V + I+F I L +V+ G C+F
Sbjct: 227 KNSWKKVAKVNIVFLIFLIIVYSVGCCAF 255
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 27.9 bits (59), Expect = 7.5
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = +2
Query: 128 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWR 229
S+ ++ D +P D P P S KR W +R
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 157
>At4g30430.1 68417.m04322 senescence-associated family protein
similar to senescence-associated protein 5 [Hemerocallis
hybrid cultivar] gi|3551954|gb|AAC34855
Length = 272
Score = 27.5 bits (58), Expect = 9.9
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 203 KRPWKIVWRNVILFFILLSVVFMEGTCSF 289
K WK V I+F +LL VV+ G C+F
Sbjct: 227 KAAWKRVAIVNIIFLVLLVVVYAMGCCAF 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,872,091
Number of Sequences: 28952
Number of extensions: 451375
Number of successful extensions: 1344
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1277
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1343
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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