Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0730
         (789 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD...    87   2e-17
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy...    55   5e-08
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy...    34   0.094
At5g63910.1 68418.m08025 expressed protein                             32   0.38 
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy...    32   0.38 
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy...    32   0.38 
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein...    31   1.2  
At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera...    29   2.7  
At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera...    29   2.7  
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr...    29   3.5  
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit...    28   8.1  

>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
           / GPDH strong similarity to  SP|P52425
           Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
           {Cuphea lanceolata}; contains Pfam profile PF01210:
           NAD-dependent glycerol-3-phosphate dehydrogenase
          Length = 400

 Score = 86.6 bits (205), Expect = 2e-17
 Identities = 43/83 (51%), Positives = 58/83 (69%)
 Frame = +2

Query: 257 THENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAAL 436
           T+ENVKYLPG KL  NVVA PD+  A KDA++L+FV PHQF+  IC  L GKI     A+
Sbjct: 109 TNENVKYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAI 168

Query: 437 SLIKGFDIAEGGGIDLISHIITR 505
           SL+KG ++ + G   +IS +I++
Sbjct: 169 SLVKGMEVKKEGPC-MISSLISK 190



 Score = 83.4 bits (197), Expect = 2e-16
 Identities = 43/88 (48%), Positives = 52/88 (59%), Gaps = 1/88 (1%)
 Frame = +1

Query: 502 KMLKIPCAVLMGANIASEVAEEKFCETTIGCRDVM-LAPLMRDIIQTDYFRXXXXXXXXX 678
           K L I C VLMGANIA+E+A EKF E T+G R    +A     +  T YF          
Sbjct: 190 KQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQLFSTPYFMVTPVHDVEG 249

Query: 679 XXICGALKNIVAVGAGFVDGLGYGDNTK 762
             +CG LKN+VA+ AGFVDGL  G+NTK
Sbjct: 250 VELCGTLKNVVAIAAGFVDGLEMGNNTK 277



 Score = 71.3 bits (167), Expect = 7e-13
 Identities = 30/55 (54%), Positives = 41/55 (74%), Gaps = 1/55 (1%)
 Frame = +3

Query: 96  KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII-EGKKLTEIINK 257
           K+KV +VGSGNWGS  AK++  NA  L +F D V MWV+EE++  G+KL ++INK
Sbjct: 54  KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVLPNGEKLNDVINK 108


>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein low similarity to SP|Q26756
           Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
           (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
           Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 420

 Score = 55.2 bits (127), Expect = 5e-08
 Identities = 26/67 (38%), Positives = 34/67 (50%)
 Frame = +2

Query: 260 HENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALS 439
           H N KY P HKLP NV+A  D   A  DAD  +  VP QF  +    +   + P    +S
Sbjct: 132 HHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFIS 191

Query: 440 LIKGFDI 460
           L KG ++
Sbjct: 192 LSKGLEL 198



 Score = 49.2 bits (112), Expect = 3e-06
 Identities = 24/81 (29%), Positives = 39/81 (48%)
 Frame = +1

Query: 517 PCAVLMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYFRXXXXXXXXXXXICGA 696
           P   L G + A E+         +  +D  LA  ++ ++ + Y R           I GA
Sbjct: 218 PFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSYLRINTSSDVTGVEIAGA 277

Query: 697 LKNIVAVGAGFVDGLGYGDNT 759
           LKN++A+ AG VDG+  G+N+
Sbjct: 278 LKNVLAIAAGIVDGMNLGNNS 298


>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 455

 Score = 34.3 bits (75), Expect = 0.094
 Identities = 22/68 (32%), Positives = 33/68 (48%)
 Frame = +1

Query: 529 LMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYFRXXXXXXXXXXXICGALKNI 708
           L G NIASEV  +++    I   +    PL + + Q+ +F            + G LKN+
Sbjct: 221 LGGPNIASEVYNKEYANARICGSEKWRKPLGKFLRQS-HFIVWDNSDLITHEVMGGLKNV 279

Query: 709 VAVGAGFV 732
            A+GAG V
Sbjct: 280 YAIGAGMV 287


>At5g63910.1 68418.m08025 expressed protein
          Length = 500

 Score = 32.3 bits (70), Expect = 0.38
 Identities = 16/46 (34%), Positives = 26/46 (56%)
 Frame = +3

Query: 105 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKLT 242
           VCIVGSG  GS++A  +   + S    + ++ M+   EI+ G+  T
Sbjct: 38  VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGGRMRT 83


>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 462

 Score = 32.3 bits (70), Expect = 0.38
 Identities = 20/71 (28%), Positives = 32/71 (45%)
 Frame = +1

Query: 529 LMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYFRXXXXXXXXXXXICGALKNI 708
           L G NIA+E+  +++    I        PL +  ++  +F            + G LKN+
Sbjct: 229 LGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHFIVWDNSDLVTHEVMGGLKNV 287

Query: 709 VAVGAGFVDGL 741
            A+GAG V  L
Sbjct: 288 YAIGAGMVAAL 298


>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein weak similarity to
           SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
           (EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
           PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 462

 Score = 32.3 bits (70), Expect = 0.38
 Identities = 20/71 (28%), Positives = 32/71 (45%)
 Frame = +1

Query: 529 LMGANIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYFRXXXXXXXXXXXICGALKNI 708
           L G NIA+E+  +++    I        PL +  ++  +F            + G LKN+
Sbjct: 229 LGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHFIVWDNSDLVTHEVMGGLKNV 287

Query: 709 VAVGAGFVDGL 741
            A+GAG V  L
Sbjct: 288 YAIGAGMVAAL 298


>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
           contains Pfam domain PF00098: Zinc knuckle
          Length = 474

 Score = 30.7 bits (66), Expect = 1.2
 Identities = 12/39 (30%), Positives = 17/39 (43%)
 Frame = +2

Query: 647 SGSWWWTMRTQSKYVER*RTLWQWVRVSWMASATVITQS 763
           S  WWW     +++ ER   +  W R+ W   A    QS
Sbjct: 151 SALWWWDQDEYNRWYERRAPIRTWERLKWNMCAKYSPQS 189


>At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 481

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 17/65 (26%), Positives = 30/65 (46%)
 Frame = +3

Query: 60  DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEGKKL 239
           D  +LD  ++QP   V  +  G+ G   AK +   A  L   + R  +WV    ++G   
Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDGSCC 308

Query: 240 TEIIN 254
           +E ++
Sbjct: 309 SEYVS 313


>At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 487

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = +3

Query: 69  ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 230
           +LD  +KQP   V  +  G+ GS  AK +   A  L   + R  +WV    ++G
Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDG 310


>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
           protein contains Pfam PF00646: F-box domain; contains
           Pfam PF01344 : Kelch motif; similar to SKP1 interacting
           partner 6 (GI:10716957) [Arabidopsis thaliana]
          Length = 387

 Score = 29.1 bits (62), Expect = 3.5
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +3

Query: 528 INGSQYCIGGC*GKILRNDHWL 593
           I+G  Y IGGC   + RNDHW+
Sbjct: 181 IDGRIYVIGGC---VKRNDHWI 199


>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
           to SP|Q10567 Adapter-related protein complex 1 beta 1
           subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
           [Drosophila melanogaster] GI:434902; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 893

 Score = 27.9 bits (59), Expect = 8.1
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -2

Query: 614 GASITSRQPMVVSQNFSSATSDAIL 540
           GAS  +  PMV+SQN S+ ++ ++L
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVL 757


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,022,634
Number of Sequences: 28952
Number of extensions: 310890
Number of successful extensions: 999
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 960
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 997
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)