Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0612
(737 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 134 8e-32
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 50 1e-06
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 50 1e-06
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 35 0.065
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 35 0.065
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 29 2.4
At5g24680.1 68418.m02917 expressed protein similar to unknown pr... 29 3.2
At1g30570.1 68414.m03740 protein kinase family protein contains ... 29 3.2
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 29 4.3
At5g45720.1 68418.m05621 hypothetical protein 28 5.6
At3g09710.1 68416.m01150 calmodulin-binding family protein low s... 28 5.6
At2g39980.1 68415.m04913 transferase family protein contains Pfa... 28 5.6
At5g40740.1 68418.m04944 expressed protein 28 7.4
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 28 7.4
At4g11420.1 68417.m01840 eukaryotic translation initiation facto... 27 9.8
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 27 9.8
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 27 9.8
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 9.8
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 9.8
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 9.8
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 134 bits (323), Expect = 8e-32
Identities = 58/74 (78%), Positives = 65/74 (87%)
Frame = +1
Query: 514 MVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHP 693
MVIVS ILERD H ++LWNTAV+I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHP
Sbjct: 176 MVIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHP 235
Query: 694 VFATRYGKIAVNIC 735
VF T +GKIAVNIC
Sbjct: 236 VFETVFGKIAVNIC 249
Score = 112 bits (269), Expect = 3e-25
Identities = 52/89 (58%), Positives = 66/89 (74%)
Frame = +2
Query: 242 PENCEVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFC 421
P VG++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W MPFAFC
Sbjct: 87 PRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTMPFAFC 146
Query: 422 TREKQPWCEFAESAEDGPTTTFLRELAIK 508
TRE++ WCEFAE DG +T FL+ELA K
Sbjct: 147 TRERR-WCEFAEPV-DGESTKFLQELAKK 173
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 50.4 bits (115), Expect = 1e-06
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +1
Query: 568 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNIC 735
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC
Sbjct: 131 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAIC 186
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.4 bits (115), Expect = 1e-06
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +1
Query: 568 WNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNIC 735
+N+ +I G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC
Sbjct: 104 YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAIC 159
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 34.7 bits (76), Expect = 0.065
Identities = 24/68 (35%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 711
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 712 GKIAVNIC 735
G+I + IC
Sbjct: 235 GRIGIGIC 242
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +2
Query: 302 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 475
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 476 TTTFLRELA 502
+T L E++
Sbjct: 154 STAMLSEVS 162
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 34.7 bits (76), Expect = 0.065
Identities = 24/68 (35%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 711
E+ D L+NT V G + KHRK H IP F ES G T + T
Sbjct: 176 ERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDTDV 234
Query: 712 GKIAVNIC 735
G+I + IC
Sbjct: 235 GRIGIGIC 242
Score = 33.5 bits (73), Expect = 0.15
Identities = 19/69 (27%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +2
Query: 302 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 475
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 476 TTTFLRELA 502
+T L E++
Sbjct: 154 STAMLSEVS 162
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.5 bits (63), Expect = 2.4
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 726
EK L+ TA+ S G +GKHRK +P + + PV+ T GKI
Sbjct: 127 EKDGYTLYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGA 183
Query: 727 NIC 735
IC
Sbjct: 184 AIC 186
>At5g24680.1 68418.m02917 expressed protein similar to unknown
protein (pir||T38384)
Length = 399
Score = 29.1 bits (62), Expect = 3.2
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = -3
Query: 522 DHHCVLMASSRRKVVVGPSSADSANSHHGCFSLVQ-NAKGMFHN 394
DHH L +S PSS+ SA+S + SLVQ K F++
Sbjct: 37 DHHLALQLASDDHSSDDPSSSSSASSSNNVASLVQLQTKSQFYS 80
>At1g30570.1 68414.m03740 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 849
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +3
Query: 18 NVRHHSQSWRKTHSLESIINNNLTG 92
N+ + SW+K +LESII++NL G
Sbjct: 731 NLAEWALSWQKQRNLESIIDSNLRG 755
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 28.7 bits (61), Expect = 4.3
Identities = 20/63 (31%), Positives = 28/63 (44%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 726
EK L+ TA+ S G +GKHRK +P + + PV+ T GK+
Sbjct: 120 EKDGYTLYCTALFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 176
Query: 727 NIC 735
IC
Sbjct: 177 AIC 179
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 5.6
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +1
Query: 583 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 708
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At3g09710.1 68416.m01150 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 454
Score = 28.3 bits (60), Expect = 5.6
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +1
Query: 103 KSSTEFILGGGITLKSN*KNPRSLQLRRLTSTSPHTLSR-PRTSRPDPREL*GRNSSAFH 279
K+ST GG T KS+ +N + + T ++ T +R PR RP P + ++S
Sbjct: 290 KNSTNRNSQGGETAKSSNRNKLNSSTKPNTPSASSTATRNPRKKRPIPSSIKSKSSDDEA 349
Query: 280 RGAHRS 297
+ + R+
Sbjct: 350 KSSERN 355
>At2g39980.1 68415.m04913 transferase family protein contains Pfam
profile PF02458 transferase family
Length = 482
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = -2
Query: 595 CH*LQPQCSKVCPNASRPFLISRTRSPLRL 506
CH +Q C CPN P LIS + L +
Sbjct: 39 CHYIQKGCLFTCPNLPLPALISHLKHSLSI 68
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 538 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 663
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor [Homo
sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1706
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 493 GTRHQDAMVIVSSILERDEKHSDILWNTAVVISD 594
G D++ I+S ++ R +KH D+L V+SD
Sbjct: 1478 GDLDDDSLHIMSYVVSRTKKHIDVLSQIVEVVSD 1511
>At4g11420.1 68417.m01840 eukaryotic translation initiation factor 3
subunit 10 / eIF-3 theta / eIF3a (TIF3A1) identical to
eukaryotic translation initiation factor 3 subunit 10
(eIF-3 theta) (Eukaryotic translation initiation factor
3 large subunit) (eIF3a) (p114). [Arabidopsis thaliana]
SWISS-PROT:Q9LD55
Length = 987
Score = 27.5 bits (58), Expect = 9.8
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +3
Query: 27 HHSQSWRKTHSLESIINNNLTGRDLE 104
+H+ +W K SL+ N NL+ +DL+
Sbjct: 292 YHAYAWFKLFSLQKNFNKNLSQKDLQ 317
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 726
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 5 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 61
Query: 727 NIC 735
IC
Sbjct: 62 AIC 64
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = +1
Query: 547 EKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAV 726
EK L+ T + S G +GKHRK +P + + PV+ T GK+
Sbjct: 127 EKEGYTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGA 183
Query: 727 NIC 735
IC
Sbjct: 184 AIC 186
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -2
Query: 643 RLSECGSCDVSRSRFRCH*LQPQCSKVCPNASRPFLISRTRSP 515
RL G C + R CH ++ SK R LI + R+P
Sbjct: 332 RLQGVGQCLIREYRMVCHVMKGDISKDFVEGCRAVLIDKDRNP 374
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 9.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +1
Query: 586 ISDTGNVIGKHRKNHIP 636
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 9.8
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +1
Query: 586 ISDTGNVIGKHRKNHIP 636
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,771,232
Number of Sequences: 28952
Number of extensions: 361950
Number of successful extensions: 1139
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1099
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1137
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -