BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0811 (720 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 29 3.1 >At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) identical to acyl-activating enzyme 14 [Arabidopsis thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}; contains Pfam profile PF00501: AMP-binding enzyme; identical to cDNA acyl-activating enzyme 14 (At1g30520) GI:29893263 Length = 560 Score = 29.1 bits (62), Expect = 3.1 Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = +1 Query: 457 RTGRYHHPAYFCREA-VMRFGLKGGAAVVTILETSELISQDGWRIYVVDVYGLQ*PLNTR 633 RTGR A ++R GL+ G V S+L + W + V V G+ PLN R Sbjct: 33 RTGREFVDGVLSLAAGLIRLGLRNGDVVSIAAFNSDLFLE--WLLAVALVGGVVAPLNYR 90 Query: 634 WAV 642 W++ Sbjct: 91 WSL 93 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,271,440 Number of Sequences: 28952 Number of extensions: 324007 Number of successful extensions: 532 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 440 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 532 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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