Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-1292
         (648 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g19900.1 68415.m02326 malate oxidoreductase, putative similar...   186   8e-48
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar...   182   1e-46
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar...   179   1e-45
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar...   177   7e-45
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar...   145   2e-35
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar...   145   3e-35
At5g59080.1 68418.m07402 expressed protein                             28   4.7  
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal...    27   8.1  
At3g25910.1 68416.m03230 expressed protein                             27   8.1  
At1g40115.1 68414.m04755 hypothetical protein                          27   8.1  

>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P51615) {Vitis vinifera}
          Length = 581

 Score =  186 bits (454), Expect = 8e-48
 Identities = 90/159 (56%), Positives = 114/159 (71%)
 Frame = +1

Query: 88  SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 267
           SG   L+ P  NKG+AFT +ER    + GLLPP V  Q+ Q +    +I +Y+ PL KY+
Sbjct: 41  SGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYM 100

Query: 268 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 447
            L  L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+GLFI++ DKG +
Sbjct: 101 ALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKI 160

Query: 448 YDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCG 564
            DVLKNWPE +++ IVVTDGERILGLGDLG    G P G
Sbjct: 161 LDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVG 199



 Score = 53.2 bits (122), Expect = 1e-07
 Identities = 26/42 (61%), Positives = 30/42 (71%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG   G     GKLALY+ALGG++P  CLP+TIDVGTN
Sbjct: 185 GLGDLG-CQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTN 225


>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP:P12628) {Phaseolus vulgaris}
          Length = 588

 Score =  182 bits (444), Expect = 1e-46
 Identities = 88/169 (52%), Positives = 117/169 (69%)
 Frame = +1

Query: 76  TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 255
           TS  SG   ++ P  NKG+AFT +ER    I GLLPP V +Q+ Q      ++ +Y  PL
Sbjct: 44  TSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPL 103

Query: 256 NKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 435
            +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +
Sbjct: 104 QRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKE 163

Query: 436 KGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCGANSRST 582
           KG + +VLKNWP+  ++ IVVTDGERILGLGDLG    G P G  S  T
Sbjct: 164 KGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 212



 Score = 54.4 bits (125), Expect = 6e-08
 Identities = 28/42 (66%), Positives = 30/42 (71%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG   G     GKL+LYTALGGI+P  CLPITIDVGTN
Sbjct: 192 GLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTN 232


>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
           NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
           (SP|P12628) {Phaseolus vulgaris}
          Length = 588

 Score =  179 bits (436), Expect = 1e-45
 Identities = 84/169 (49%), Positives = 118/169 (69%)
 Frame = +1

Query: 76  TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 255
           TS  SG   ++ P  NKG+AFT +ER    + GLLPP + +Q+ Q      ++ +Y  PL
Sbjct: 44  TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPL 103

Query: 256 NKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 435
            +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++
Sbjct: 104 QRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNE 163

Query: 436 KGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCGANSRST 582
           KG + +VLKNWP+  ++ IVVTDGERILGLGDLG    G P G  S  T
Sbjct: 164 KGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 212



 Score = 54.4 bits (125), Expect = 6e-08
 Identities = 28/42 (66%), Positives = 30/42 (71%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG   G     GKL+LYTALGGI+P  CLPITIDVGTN
Sbjct: 192 GLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTN 232


>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
           malate oxidoreductase (NADP-dependent malic enzyme)
           GB:P34105 (Populus balsamifera subsp. trichocarpa)
          Length = 646

 Score =  177 bits (430), Expect = 7e-45
 Identities = 82/165 (49%), Positives = 117/165 (70%)
 Frame = +1

Query: 88  SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 267
           SG   L+ P  NKG+AF+  ER    + GLLPP V +Q+ QV+    ++ +Y+ PL KY+
Sbjct: 106 SGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYM 165

Query: 268 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 447
            +M L + NE LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+GLFI++ +KG +
Sbjct: 166 AMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKI 225

Query: 448 YDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCGANSRST 582
           ++VL+NWPE +++ IVVTDGERILGLGDLG    G P G  S  T
Sbjct: 226 HEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 270



 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 26/42 (61%), Positives = 30/42 (71%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG   G     GKL+LYTALGG++P  CLP+TIDVGTN
Sbjct: 250 GLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTN 290


>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
           tuberosum}
          Length = 607

 Score =  145 bits (352), Expect = 2e-35
 Identities = 78/168 (46%), Positives = 96/168 (57%), Gaps = 10/168 (5%)
 Frame = +1

Query: 91  GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN------- 249
           G D L  P  NK   F + ER  LGI GLLPPRV T  +Q +    S    EN       
Sbjct: 39  GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPE 98

Query: 250 ---PLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLF 420
               L K+  L  L DRNE L+YR + DN+ +  PI+YTPTVGL CQ +  +YRRPRG++
Sbjct: 99  NVVALAKWRMLNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMY 158

Query: 421 ITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCG 564
            +  DKG +  ++ NWP   V  IV+TDG RILGLGDLG    G P G
Sbjct: 159 FSAKDKGEMMSMIYNWPAPQVDMIVITDGSRILGLGDLGVQGIGIPIG 206



 Score = 39.1 bits (87), Expect = 0.002
 Identities = 21/42 (50%), Positives = 25/42 (59%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG + G     GKL +Y A  GI P + LPI +DVGTN
Sbjct: 192 GLGDLG-VQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTN 232


>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
           NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
           precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
           tuberosum}
          Length = 623

 Score =  145 bits (351), Expect = 3e-35
 Identities = 74/159 (46%), Positives = 96/159 (60%), Gaps = 10/159 (6%)
 Frame = +1

Query: 91  GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYE-------- 246
           GLD L  P  NKG AFT+ ER  L + GLLPP V   E+Q+      + R E        
Sbjct: 46  GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPS 105

Query: 247 --NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLF 420
             N L K+  L  L DRNE ++Y+ + +N+ E  PIVYTPTVGL CQ +  ++RRPRG++
Sbjct: 106 DPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMY 165

Query: 421 ITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLG 537
            +  D+G +  ++ NWP   V  IVVTDG RILGLGDLG
Sbjct: 166 FSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLG 204



 Score = 40.7 bits (91), Expect = 8e-04
 Identities = 22/42 (52%), Positives = 25/42 (59%)
 Frame = +3

Query: 519 GSGRLGRMPGWASLWGKLALYTALGGIKPHQCLPITIDVGTN 644
           G G LG + G     GKL LY A  GI P + LP+ IDVGTN
Sbjct: 199 GLGDLG-VHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTN 239


>At5g59080.1 68418.m07402 expressed protein
          Length = 135

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 21/65 (32%), Positives = 27/65 (41%)
 Frame = +1

Query: 49  LWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKL 228
           L+GS D  P S  SG+     P  +KG A         G        +  QE++VE C L
Sbjct: 21  LFGSKDPSPPSSSSGIFSTMFPHPSKGSA-RDGSNSKHGSQAQRRESLNAQEDRVEPCHL 79

Query: 229 SIDRY 243
           S   Y
Sbjct: 80  SSSLY 84


>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha
           catalytic subunit, putative similar to SP|O48653 DNA
           polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza
           sativa}; contains Pfam profiles: PF03175 DNA polymerase
           type B, organellar and viral, PF00136 DNA polymerase
           family B, PF03104 DNA polymerase family B, exonuclease
           domain
          Length = 1492

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 23/72 (31%), Positives = 31/72 (43%)
 Frame = +1

Query: 388 GLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGERILGLGDLGACRDGHPCGA 567
           G   R  + +F T+ D+   YD L +    + R  VV DGE     GDLG   +G     
Sbjct: 44  GYDIRLQKPIFDTVDDEE--YDALVSRRREEARGFVVEDGEG----GDLGYLDEGEEEDW 97

Query: 568 NSRSTPRSEASS 603
           +  S P S   S
Sbjct: 98  SKPSGPESTDES 109


>At3g25910.1 68416.m03230 expressed protein
          Length = 372

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = -3

Query: 550 HPGMRPSRPDPEYVLRRLQQWR 485
           HPG+RPS  DPE    R + WR
Sbjct: 225 HPGVRPSEADPE----RQRSWR 242


>At1g40115.1 68414.m04755 hypothetical protein
          Length = 150

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 19/58 (32%), Positives = 31/58 (53%)
 Frame = -2

Query: 221 HSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLVGCPSPEPHN 48
           HS+T SS    RG ++   P + RSS +  + + RP    ++RP   +   PSP+ H+
Sbjct: 19  HSTTRSS----RGHHHFTSPLAPRSSAI--ISITRPSLDHFTRPRGRVPSSPSPDRHS 70


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,874,699
Number of Sequences: 28952
Number of extensions: 369600
Number of successful extensions: 1060
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1021
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1058
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)