Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-2398
         (700 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1al...   452   e-129
X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alp...   437   e-125
AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor 1-a...   334   5e-94
EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor 1-a...   301   4e-84
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    35   6e-04
AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9 methylt...    24   1.2  
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...    23   3.7  
D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.    22   4.9  
AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic ac...    21   8.5  

>AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1alpha
           F2 protein.
          Length = 461

 Score =  452 bits (1115), Expect = e-129
 Identities = 214/231 (92%), Positives = 216/231 (93%)
 Frame = +3

Query: 6   MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 185
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 186 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGT 365
           DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAGT
Sbjct: 61  DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120

Query: 366 GEFEAGISKNGQTREHALLAFTLGVKQLIVRVNKMDSTEPPYSEPRFXEIKXEVSSYIKK 545
           GEFEAGISKNGQTREHALLAFTLGVKQLIV VNKMDSTEPPYSE RF EIK EVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180

Query: 546 IGYNPXAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADXKCLIXA 698
           IGYNP AVAFVPISGWHGDNMLE S+KMPWF GW VER E K + KCLI A
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 231


>X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alpha
           protein.
          Length = 461

 Score =  437 bits (1077), Expect = e-125
 Identities = 206/231 (89%), Positives = 212/231 (91%)
 Frame = +3

Query: 6   MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 185
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 186 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGT 365
           DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAG 
Sbjct: 61  DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120

Query: 366 GEFEAGISKNGQTREHALLAFTLGVKQLIVRVNKMDSTEPPYSEPRFXEIKXEVSSYIKK 545
           GEFEAGISKNGQTREHALLAFTLGVKQLIV VNKMD T+PPYSE RF EIK EVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180

Query: 546 IGYNPXAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADXKCLIXA 698
           IGYN  +VAFVPISGWHGDNMLEPS K PW+ GW+VER +  AD K LI A
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEA 231


>AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor
           1-alpha protein.
          Length = 274

 Score =  334 bits (821), Expect = 5e-94
 Identities = 158/174 (90%), Positives = 160/174 (91%)
 Frame = +3

Query: 177 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVA 356
           WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVA
Sbjct: 1   WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60

Query: 357 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVRVNKMDSTEPPYSEPRFXEIKXEVSSY 536
           AGTGEFEAGISKNGQTREHALLAFTLGVKQLIV VNKMDSTEPPYSE RF EIK EVSSY
Sbjct: 61  AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120

Query: 537 IKKIGYNPXAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADXKCLIXA 698
           IKKIGYNP AVAFVPISGWHGDNMLE S+KMPWF GW VER E K + KCLI A
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 174


>EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor
           1-alpha protein.
          Length = 172

 Score =  301 bits (739), Expect = 4e-84
 Identities = 142/158 (89%), Positives = 144/158 (91%)
 Frame = +3

Query: 225 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGEFEAGISKNGQT 404
           DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1   DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60

Query: 405 REHALLAFTLGVKQLIVRVNKMDSTEPPYSEPRFXEIKXEVSSYIKKIGYNPXAVAFVPI 584
           REHALLAFTLGVKQLIV VNKMDSTEPPYSE RF EIK EVSSYIKKIGYNP AVAFVPI
Sbjct: 61  REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120

Query: 585 SGWHGDNMLEPSTKMPWFXGWQVERXESKADXKCLIXA 698
           SGWHGDNMLE S+KMPWF GW VER E K + KCLI A
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 158


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 35.1 bits (77), Expect = 6e-04
 Identities = 26/70 (37%), Positives = 33/70 (47%)
 Frame = +3

Query: 264 VTIIDAPGHRDFIKNMITGTSXADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 443
           VT +D PGH  FI     G    D  VL+VAA  G  E       QT +   +A    V 
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246

Query: 444 QLIVRVNKMD 473
            +IV +NK+D
Sbjct: 247 PIIVAINKID 256



 Score = 25.4 bits (53), Expect = 0.52
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = +3

Query: 18  KTHINIVVIGHVDSGKST 71
           K H  + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160


>AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9
          methyltransferase protein.
          Length = 683

 Score = 24.2 bits (50), Expect = 1.2
 Identities = 11/15 (73%), Positives = 11/15 (73%)
 Frame = +3

Query: 27 INIVVIGHVDSGKST 71
          INI  IGHV  GKST
Sbjct: 43 INIGTIGHVAHGKST 57


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score = 22.6 bits (46), Expect = 3.7
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +3

Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLI 350
           T KYY    D P +  FIKN+   ++ +D   LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327


>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score = 22.2 bits (45), Expect = 4.9
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSXADCAVLI 350
           T KYY    D P +  FIKN+   ++ +D   L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327


>AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic
           acetylcholine Apisa7-2 subunit protein.
          Length = 461

 Score = 21.4 bits (43), Expect = 8.5
 Identities = 8/24 (33%), Positives = 13/24 (54%)
 Frame = -3

Query: 626 FG*RLQHVVSVPSRNGHESDSXWV 555
           FG  L H++ V  +N   + + WV
Sbjct: 35  FGLSLHHIIDVDEKNQILTTNCWV 58


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 186,665
Number of Sequences: 438
Number of extensions: 3624
Number of successful extensions: 14
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21439440
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)