BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2255
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces pom... 31 0.097
SPAC19A8.14 |||aminoacyl-tRNA hydrolase |Schizosaccharomyces pom... 28 1.2
SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 3.7
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 26 4.8
SPBC17A3.09c |||lipoate-protein ligase A |Schizosaccharomyces po... 25 6.4
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 25 8.5
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 25 8.5
>SPAC1565.04c |ste4||adaptor protein Ste4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 264
Score = 31.5 bits (68), Expect = 0.097
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +2
Query: 275 DVTQLAWEDLEDMGIVRLGHQKKILLAIKRVK 370
D+ L+ DL DMGI +GH+ IL AI+ +K
Sbjct: 40 DIDLLSSNDLRDMGIESVGHRIDILSAIQSMK 71
>SPAC19A8.14 |||aminoacyl-tRNA hydrolase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 205
Score = 27.9 bits (59), Expect = 1.2
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 105 PTAVRSSGVILVMVGRSYPASMKSRAYSS 19
P AV V+ M+GR Y + SR YSS
Sbjct: 14 PAAVLVGAVVGFMIGRKYSVADASRGYSS 42
>SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 141
Score = 26.2 bits (55), Expect = 3.7
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 198 KEPGM*SGKLSGTLRLASSAFNLLRWL 118
K+P + SG+++G L L SS F W+
Sbjct: 56 KDPRLISGRMTGALILYSSVFMRYAWM 82
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 25.8 bits (54), Expect = 4.8
Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
Frame = +1
Query: 22 GVRAALHRGWVRSADHNKDDSRGPHGCRHQETQP--PQEVKGRARQSQR 162
G R A +R W S+ + DDS G C+ P Q K + R+S +
Sbjct: 250 GGRNANYRKWYFSSSMDIDDSVGLQNCKFSSNGPKIKQAKKKQKRKSNK 298
>SPBC17A3.09c |||lipoate-protein ligase A |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 25.4 bits (53), Expect = 6.4
Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 3/45 (6%)
Frame = -3
Query: 373 VFHSFNGKQYFLLVTKPHYSHV--LKILPSELSDV-VHGXIALRH 248
VFH F Y +L+ + +SH I+ L ++ VH + RH
Sbjct: 98 VFHDFGNLNYSVLMNREEFSHTENASIMIQALRNLGVHARLNQRH 142
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 25.0 bits (52), Expect = 8.5
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = -1
Query: 186 M*SGKLSGTLRLASSAFNLLRWLGFLMPTAVRSSGV 79
M S +L +RL S+++NL RW+ AVR+ G+
Sbjct: 1098 MFSERLDNLVRLQSTSYNLNRWV------AVRTDGI 1127
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 482 YHKPPDLNFDAPXDXIMXX*SWDPIXIPXAXXQI 583
YH+P ++ F P D + WDPI P Q+
Sbjct: 351 YHQPSNV-FVPPEDPDLPAFFWDPIINPITSRQL 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,252,832
Number of Sequences: 5004
Number of extensions: 42324
Number of successful extensions: 108
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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