BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2209
(550 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 40 2e-05
DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chlor... 21 6.2
>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
protein.
Length = 1370
Score = 39.5 bits (88), Expect = 2e-05
Identities = 29/104 (27%), Positives = 47/104 (45%), Gaps = 1/104 (0%)
Frame = +1
Query: 190 LLNVELQDNPIGNVEG-PFLVSPTLQYLDLSNCNITSINSQFFDNITTLMTVDLSNNPLK 366
L + L N I +++ F L+ LDLS +TS+ D + L T+DL N +
Sbjct: 409 LNRLTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVPDALRD-LALLKTLDLGENRIS 467
Query: 367 NLATGVFDILTSIETLKLNDCKLSNISEVLFXNAAHLQEFGIGR 498
N G F L + L+L + N+S + + +LQ + R
Sbjct: 468 NFYNGSFRNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNLAR 511
Score = 36.7 bits (81), Expect = 2e-04
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +3
Query: 45 MRRLQLADNRLTRVEREAFKGLEYLIDIDLSGNNISYVDPI 167
+ L+L+DN+L V + F GL L + LSGN I+ +DP+
Sbjct: 385 LHTLELSDNKLRTVGAQLFNGLFVLNRLTLSGNAIASIDPL 425
Score = 32.3 bits (70), Expect = 0.003
Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Frame = +3
Query: 45 MRRLQLADNRLTRVEREAFKGLEYLIDIDLSGNNISYV-DPIGVLGFPRFAKRGASRQSN 221
+ RL L+ N + ++ AF+ L ++DLSGN ++ V D + L + G +R SN
Sbjct: 409 LNRLTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVPDALRDLALLKTLDLGENRISN 468
Score = 28.7 bits (61), Expect = 0.041
Identities = 14/50 (28%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +3
Query: 3 NNITTLKPFPNDIKM-RRLQLADNRLTRVEREAFKGLEYLIDIDLSGNNI 149
N +T++ D+ + + L L +NR++ +F+ L+ L + L GN+I
Sbjct: 441 NELTSVPDALRDLALLKTLDLGENRISNFYNGSFRNLDQLTGLRLIGNDI 490
Score = 27.1 bits (57), Expect = 0.13
Identities = 23/102 (22%), Positives = 48/102 (47%)
Frame = +1
Query: 172 FLDSRGLLNVELQDNPIGNVEGPFLVSPTLQYLDLSNCNITSINSQFFDNITTLMTVDLS 351
F LL + L +N I + F+ L++LD+ I S+ + + + + T+D S
Sbjct: 545 FTSIASLLLLNLSENHIEWFDYAFIPG-NLKWLDIHGNFIESLGNYYKIRDSKVKTLDAS 603
Query: 352 NNPLKNLATGVFDILTSIETLKLNDCKLSNISEVLFXNAAHL 477
+N + L+ + S+E L +N+ ++ + F + +L
Sbjct: 604 HNRITELSP--LSVPDSVELLFINNNYINLVRPNTFTDKVNL 643
Score = 25.8 bits (54), Expect = 0.29
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +3
Query: 54 LQLADNRLTRVEREAFKGLEYLIDIDLSGNNISYVDPI 167
L LA N++ VER AF+ L I L GN +S ++ +
Sbjct: 507 LNLARNKVQHVERYAFERNMRLEAIRLDGNFLSDINGV 544
Score = 21.4 bits (43), Expect = 6.2
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +1
Query: 394 LTSIETLKLNDCKLSNISEVLFXNAAHLQEFGI 492
LTS+ L ++ CK+ I E F L++ +
Sbjct: 91 LTSLHELHVHGCKVLRIPEGAFQPLLELKKLTV 123
>DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chloride
channel protein.
Length = 383
Score = 21.4 bits (43), Expect = 6.2
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +1
Query: 241 FLVSPTLQYLDLSNCNITSINSQ 309
FL PT+ Y ++ +I SIN +
Sbjct: 25 FLGQPTIVYFHVTVLSIDSINEE 47
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 146,226
Number of Sequences: 438
Number of extensions: 3026
Number of successful extensions: 9
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9
length of database: 146,343
effective HSP length: 54
effective length of database: 122,691
effective search space used: 15704448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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