Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2136
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 171 9e-44
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 171 9e-44
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 171 9e-44
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.69
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 26 3.7
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 26 3.7
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 4.8
SPCC736.09c |||TRAX |Schizosaccharomyces pombe|chr 3|||Manual 25 6.4
SPBC887.10 |mcs4||two-component response regulator |Schizosaccha... 25 6.4
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 25 8.5
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 171 bits (415), Expect = 9e-44
Identities = 90/192 (46%), Positives = 119/192 (61%)
Frame = +3
Query: 6 RRGAGSVFGFSHXRRGKALPKLRSLDYG*TSWXHQGSC*GI*SMTLVEVHLWLLYTFRDP 185
+R +G +F +H R K +LR+LD+ +G I L + FR+P
Sbjct: 8 QRKSGGIFQ-AHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKV-AFRNP 65
Query: 186 YKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEXKMGDRGRL 365
Y ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+E K GDRG L
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 366 ARAXGNFATVIGHNPDAKRTRSKATRLEPRKVLPSSNRXMXXXXXXXXRIDKPILKAGRA 545
R+ GN+ ++GH+ D +TR K +KV+PSS R + RIDKP+LKAGRA
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPS-GAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRA 184
Query: 546 YPKXKVKRNCWP 581
+ K +VKRNCWP
Sbjct: 185 FHKYRVKRNCWP 196
Score = 34.7 bits (76), Expect = 0.010
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 110 RELLRDIIHDPGRGAPLAVVHF 175
R +++ IIHDPGRGAPLA V F
Sbjct: 41 RGVVQKIIHDPGRGAPLAKVAF 62
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 171 bits (415), Expect = 9e-44
Identities = 90/192 (46%), Positives = 119/192 (61%)
Frame = +3
Query: 6 RRGAGSVFGFSHXRRGKALPKLRSLDYG*TSWXHQGSC*GI*SMTLVEVHLWLLYTFRDP 185
+R +G +F +H R K +LR+LD+ +G I L + FR+P
Sbjct: 8 QRKSGGIFQ-AHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKV-AFRNP 65
Query: 186 YKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEXKMGDRGRL 365
Y ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+E K GDRG L
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 366 ARAXGNFATVIGHNPDAKRTRSKATRLEPRKVLPSSNRXMXXXXXXXXRIDKPILKAGRA 545
R+ GN+ ++GH+ D +TR K +KV+PSS R + RIDKP+LKAGRA
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPS-GAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRA 184
Query: 546 YPKXKVKRNCWP 581
+ K +VKRNCWP
Sbjct: 185 FHKYRVKRNCWP 196
Score = 34.7 bits (76), Expect = 0.010
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 110 RELLRDIIHDPGRGAPLAVVHF 175
R +++ IIHDPGRGAPLA V F
Sbjct: 41 RGVVQKIIHDPGRGAPLAKVAF 62
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 171 bits (415), Expect = 9e-44
Identities = 90/192 (46%), Positives = 119/192 (61%)
Frame = +3
Query: 6 RRGAGSVFGFSHXRRGKALPKLRSLDYG*TSWXHQGSC*GI*SMTLVEVHLWLLYTFRDP 185
+R +G +F +H R K +LR+LD+ +G I L + FR+P
Sbjct: 8 QRKSGGIFQ-AHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKV-AFRNP 65
Query: 186 YKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEXKMGDRGRL 365
Y ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+E K GDRG L
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 366 ARAXGNFATVIGHNPDAKRTRSKATRLEPRKVLPSSNRXMXXXXXXXXRIDKPILKAGRA 545
R+ GN+ ++GH+ D +TR K +KV+PSS R + RIDKP+LKAGRA
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPS-GAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRA 184
Query: 546 YPKXKVKRNCWP 581
+ K +VKRNCWP
Sbjct: 185 FHKYRVKRNCWP 196
Score = 34.7 bits (76), Expect = 0.010
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 110 RELLRDIIHDPGRGAPLAVVHF 175
R +++ IIHDPGRGAPLA V F
Sbjct: 41 RGVVQKIIHDPGRGAPLAKVAF 62
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 28.7 bits (61), Expect = 0.69
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2
Query: 170 HFPRSIQVQDKEGALHCSR 226
H+PRSI V ++G L C+R
Sbjct: 178 HYPRSILVDSEDGVLRCAR 196
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 26.2 bits (55), Expect = 3.7
Identities = 15/47 (31%), Positives = 25/47 (53%)
Frame = -2
Query: 287 ISNFKSCFLSTINKLACVEPFGSNEELLPCLELVWIAESVQQPKVHL 147
ISNF + +L + K+A V+P EL P L E ++ ++H+
Sbjct: 165 ISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKKLQIHV 211
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 3.7
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = -2
Query: 335 KIAHNGTLRHSSNRHHISNFKSCFLSTINKLACVEPFGSNEELLPCLELVWIAESVQQPK 156
K+ G +RH +SNF L I K+A V+P EL P L E ++
Sbjct: 154 KLLETGKVRHIG----LSNFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLG 209
Query: 155 VHL 147
+H+
Sbjct: 210 IHV 212
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 4.8
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 358 PLSPIFXSRLHTMVPSGIAPTGITFPTSRVAFF 260
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
>SPCC736.09c |||TRAX |Schizosaccharomyces pombe|chr 3|||Manual
Length = 231
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/39 (38%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
Frame = -2
Query: 452 WLQTGSFTSCTLSIRIVSNHSGEVSXGTCQTT-SITHFL 339
WLQTG+ SC S +S + + G C T I FL
Sbjct: 104 WLQTGTLLSCKDSSFRISINFIDYVLGVCDMTGEIMRFL 142
>SPBC887.10 |mcs4||two-component response regulator
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 522
Score = 25.4 bits (53), Expect = 6.4
Identities = 14/57 (24%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Frame = +2
Query: 122 RDIIHDPGRGAPLAVVHFPRSIQVQDKEGALHCS-RRALHRPICLLWKESNS*SWKC 289
+D +++ +P+ +V S + D+ AL L +P+ L+W E W C
Sbjct: 450 KDQLNENKFQSPVIIVALTASSLMADRNEALAAGCNDFLTKPVSLVWLEKKITEWGC 506
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = -2
Query: 419 LSIRIVSNHSGEVSXGTCQTTSITHFLFKIAHNGTLRHSSNRH 291
L++R + N S E + GT ++HF+F + H + R+
Sbjct: 868 LTLRRMCNES-EYTIGTSNAKDVSHFVFSCPYYTHFCHPTRRY 909
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,371,913
Number of Sequences: 5004
Number of extensions: 45359
Number of successful extensions: 126
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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