Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2008
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 248 5e-67
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 150 2e-37
SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit Prp31|S... 46 4e-06
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 31 0.23
SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomy... 29 0.94
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 27 2.2
SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce... 27 2.2
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 27 3.8
SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr 1|||... 27 3.8
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 5.0
SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5 |Schizos... 26 5.0
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 6.6
SPAC22G7.09c |nup45||nucleoporin Nup45|Schizosaccharomyces pombe... 22 6.6
SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 25 8.7
>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 248 bits (608), Expect = 5e-67
Identities = 122/249 (48%), Positives = 166/249 (66%), Gaps = 2/249 (0%)
Frame = +2
Query: 8 TPEGASTVVKLKNFVKFEDXXXXXXXXXXXIEGXXXXXXXXXXXXXXXXEVQDQLLVGDS 187
+PEGAS ++KL++F KFE +EG + L+V D
Sbjct: 33 SPEGASNLLKLQSFAKFESTVDALDNVSALVEGKVSSKLSSLLEGLSDSK-SSTLVVADP 91
Query: 188 KLGSAIREK--FDLQCVSNTNVQELLRCIRSQMDSLLAGLPKKEMTAMALGLAHSLSRYK 361
KLG+AI + + + +S+++VQ+L R IR + SL++GL ++ AM+LGL+HSLSR+K
Sbjct: 92 KLGNAINKLPGLEFEIISDSSVQDLYRGIREHLSSLISGLAPSDLNAMSLGLSHSLSRHK 151
Query: 362 LKFSPDKIDTMIVQAQCLLDDLDKELNNYIMRCREWYGWHFPELGKIITDNTLFVKVVKL 541
LKFSPDK+DTMIVQA LLDDLDKELN Y MR REWYGWHFPE+GKII DN + +V+K
Sbjct: 152 LKFSPDKVDTMIVQAIALLDDLDKELNTYAMRVREWYGWHFPEMGKIIQDNLAYARVIKA 211
Query: 542 IGTRDYASKTDLSDILPEDLXEKVXGAAEISMGTEISEDDIMNIQNLXDEIIXITDYRTH 721
+G R S+TD SDILPE++ + AAEISMGTEI+E+D+ NI L D+++ + YR
Sbjct: 212 MGMRTKCSETDFSDILPEEIEATLKSAAEISMGTEITEEDLDNIVMLADQVLELASYRAQ 271
Query: 722 LTDYLXARM 748
L++YL RM
Sbjct: 272 LSEYLRNRM 280
>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 497
Score = 150 bits (363), Expect = 2e-37
Identities = 75/197 (38%), Positives = 118/197 (59%), Gaps = 6/197 (3%)
Frame = +2
Query: 176 VGDSKLGSAIREKFD-LQCVSNTNVQELLRCIRSQMDSLLAGLPKKEMTAMALGLAHSLS 352
V D L ++I+ + D ++C ++ Q+LLR IR D LL L + LGL HS S
Sbjct: 96 VQDKNLATSIKSEIDAIECDTSELTQDLLRGIRFHGDKLLKQLSPGDFERAQLGLGHSYS 155
Query: 353 RYKLKFSPDKIDTMIVQAQCLLDDLDKELNNYIMRCREWYGWHFPELGKIITDNTLFVKV 532
R K+KF+ ++ D MI+QA +LD LDK++N + MR +EWY WHFPEL KI+ DN + +
Sbjct: 156 RAKVKFNVNRNDNMIIQAIAILDQLDKDINTFAMRMKEWYSWHFPELSKIVGDNYKYAVI 215
Query: 533 VKLIGTRDYASKTDLSDIL-----PEDLXEKVXGAAEISMGTEISEDDIMNIQNLXDEII 697
V L+G + + L D+ +D+ + + A ++SMG +ISE D+ NI + + +I
Sbjct: 216 VTLVGDKTTINDEMLHDLAAVVDDDKDIAQSIINAGKVSMGQDISEIDLENILSFAERVI 275
Query: 698 XITDYRTHLTDYLXARM 748
+++YR L +YL +M
Sbjct: 276 KLSNYRKQLHNYLVQKM 292
>SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit
Prp31|Schizosaccharomyces pombe|chr 2|||Manual
Length = 518
Score = 46.4 bits (105), Expect = 4e-06
Identities = 28/119 (23%), Positives = 57/119 (47%)
Frame = +2
Query: 392 MIVQAQCLLDDLDKELNNYIMRCREWYGWHFPELGKIITDNTLFVKVVKLIGTRDYASKT 571
+IV + + ++D E+ +EWY FPEL ++ + + K V + SKT
Sbjct: 119 LIVDSNSIAMEIDDEILRLHRLVKEWYHDRFPELSSLVLNAFDYCKTVSSLLNDLDNSKT 178
Query: 572 DLSDILPEDLXEKVXGAAEISMGTEISEDDIMNIQNLXDEIIXITDYRTHLTDYLXARM 748
LS LP + A ++G + ++ I N++N + I + + + + +Y+ +R+
Sbjct: 179 KLS-FLPSATVMVIATTATTTVGKPLPDEMIKNVKNCCEAIQQLGEEKQKIIEYVQSRI 236
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 30.7 bits (66), Expect = 0.23
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = +2
Query: 428 DKELNNYIMRCREWYGWHFPELGKIITDNTLFVKVVKLIGTRDYASKTDLSDILPEDLXE 607
D++ +NY+ + E HF ITD+ V + L G+ D+ ++ SD+ + L
Sbjct: 192 DEKRHNYVRKVAEGAVQHF------ITDDKPNVAGIVLAGSADFKTELGQSDLFDQRLQS 245
Query: 608 KVXGAAEISMG 640
++ ++S G
Sbjct: 246 RIIKTVDVSYG 256
>SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 756
Score = 28.7 bits (61), Expect = 0.94
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = +2
Query: 509 DNTLFVKVVKLIGTRDYASKTDLSDILPEDLXEKVXGAAEISMGTEISE 655
DN+L ++K IG ++ L ++LPED+ G+ +S+ + E
Sbjct: 6 DNSL---IIKNIGNNEFKFVETLHELLPEDITYDDLGSLRLSLSERLQE 51
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +2
Query: 197 SAIREKFDLQCVSNTNVQELLRCIRSQMDSLLAGLPKKEMTAMALGLAHSLSRYKLK 367
SA+ + L+C++ + L +C ++ SL+ G+P T L SL K K
Sbjct: 922 SALIDPEFLKCLNEDQITALKKCHAAEHYSLILGMPGTGKTTTISSLIRSLLAKKKK 978
>SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1418
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
Frame = -1
Query: 270 RIHRSSSWTLVFDTHCKS--NFSLMALPSLESPTRS 169
R HRSS V+ HC + ++ LPSL TR+
Sbjct: 221 REHRSSGKNFVYCDHCSNVYHYDCSPLPSLNKETRN 256
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2
Query: 497 KIITDNTLFVKVVKLIGTRDYASKTDLSDILPEDLXEKVXGAAEI 631
K + N LF K K+ RD+ ++D + ++P DL E V A ++
Sbjct: 757 KRVGPNILFDKTKKM---RDFRRQSDETKLIPSDLSEYVVTAFQL 798
>SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 331
Score = 26.6 bits (56), Expect = 3.8
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -3
Query: 400 NNHSVYFVRREL*FVPRKRVSQAQSHSCHFFFR 302
+ HS Y +RREL KR+ + H C FF R
Sbjct: 119 DQHSSY-LRRELNSHREKRLQDTRVHCCLFFIR 150
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/48 (29%), Positives = 25/48 (52%)
Frame = -2
Query: 656 LQIFQFPWRSQLLX*LSLXSLQVKCQINQSWMHSLVCQ*VLPLLQTKC 513
L + Q ++LL +L ++ C + +M S + Q +LP L+T C
Sbjct: 876 LVVIQLDTDNELLVECALETIYQVCALADDFMTSRIRQDLLPRLETLC 923
>SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +3
Query: 240 LMSRNCSGVFAHRWTVS 290
+ SR C VFAHRW S
Sbjct: 8 IYSRKCECVFAHRWKPS 24
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = -1
Query: 312 SFLGSPARRLSICERIHRSSSWTLVFDT 229
S+L +P RLSI + + SSS T +T
Sbjct: 436 SYLAAPKERLSISDNMSSSSSQTATVNT 463
>SPAC22G7.09c |nup45||nucleoporin Nup45|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 22.2 bits (45), Expect(2) = 6.6
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 380 KIDTMIVQAQCLLDDLDKELNNYIMRCRE 466
+ ++ Q LDDLDKE+ + I E
Sbjct: 234 RYSSLNANTQKFLDDLDKEIFSQIQLAEE 262
Score = 21.8 bits (44), Expect(2) = 6.6
Identities = 10/41 (24%), Positives = 20/41 (48%)
Frame = +2
Query: 569 TDLSDILPEDLXEKVXGAAEISMGTEISEDDIMNIQNLXDE 691
++L + +P D+ E + +S I D+I + + DE
Sbjct: 271 SELVESVPNDVAEVQRRLSSVSTALLIDSDEIETTKRVVDE 311
>SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1150
Score = 25.4 bits (53), Expect = 8.7
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = -3
Query: 517 SVISDDFPKLWEMPTIPFTAPHD 449
++I D P LW P+ P HD
Sbjct: 408 NLIDDSSPLLWREPSFPLIEHHD 430
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,749,885
Number of Sequences: 5004
Number of extensions: 53516
Number of successful extensions: 130
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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