Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1872
(800 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 397 e-113
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 397 e-113
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 378 e-107
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 266 2e-73
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 73 3e-15
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 397 bits (978), Expect = e-113
Identities = 187/200 (93%), Positives = 191/200 (95%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKE
Sbjct: 57 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 116
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
VSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD
Sbjct: 117 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 176
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
AILPP RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA +TTEVKSVE
Sbjct: 177 AILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVE 236
Query: 541 MHHEALQEAVPGDNVGFNVK 600
MHHEALQEAVPGDNVGFNVK
Sbjct: 237 MHHEALQEAVPGDNVGFNVK 256
Score = 40.7 bits (91), Expect = 2e-05
Identities = 18/19 (94%), Positives = 19/19 (100%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQN 655
KNVSVKELRRGYVAGDS+N
Sbjct: 256 KNVSVKELRRGYVAGDSKN 274
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 397 bits (978), Expect = e-113
Identities = 187/200 (93%), Positives = 191/200 (95%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKE
Sbjct: 114 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 173
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
VSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD
Sbjct: 174 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 233
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
AILPP RPTDK LRLPLQDVYKIGGIGTVPVGRVETGVLKPG +V FAPA +TTEVKSVE
Sbjct: 234 AILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVE 293
Query: 541 MHHEALQEAVPGDNVGFNVK 600
MHHEALQEAVPGDNVGFNVK
Sbjct: 294 MHHEALQEAVPGDNVGFNVK 313
Score = 77.0 bits (181), Expect = 2e-16
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KNVSVKELRRGYVAGDS+NNPPKGAADFTAQVI LNH
Sbjct: 313 KNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 349
Score = 47.6 bits (108), Expect = 1e-07
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGC 773
PGQISNGY PVLDCHT HI C
Sbjct: 350 PGQISNGYTPVLDCHTAHIAC 370
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 378 bits (931), Expect = e-107
Identities = 177/200 (88%), Positives = 187/200 (93%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKE
Sbjct: 114 LIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKE 173
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
VSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VERK+G ADGK LIEALD
Sbjct: 174 VSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALD 233
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
AILPP+RPTDK LRLPLQDVYKIGGIGTVPVGRVETG+LKPG +V FAPA +TTEVKSVE
Sbjct: 234 AILPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVE 293
Query: 541 MHHEALQEAVPGDNVGFNVK 600
MHHEAL EA+PGDNVGFNVK
Sbjct: 294 MHHEALTEALPGDNVGFNVK 313
Score = 72.9 bits (171), Expect = 3e-15
Identities = 32/37 (86%), Positives = 35/37 (94%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KN+SVKELRRGYVAGDS+N PP+GAADFTAQVI LNH
Sbjct: 313 KNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNH 349
Score = 47.6 bits (108), Expect = 1e-07
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGC 773
PGQISNGY PVLDCHT HI C
Sbjct: 350 PGQISNGYTPVLDCHTAHIAC 370
Score = 21.8 bits (44), Expect = 7.6
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = -1
Query: 581 LSPGTASWRASWCISTDLTSVVMLAGAKTTMVPGFN 474
L PG A ++T++ SV M A T +PG N
Sbjct: 272 LKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDN 307
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 266 bits (651), Expect = 2e-73
Identities = 124/131 (94%), Positives = 126/131 (96%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKE
Sbjct: 41 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 100
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
VSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD
Sbjct: 101 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 160
Query: 361 AILPPARPTDK 393
AILPP RPTDK
Sbjct: 161 AILPPTRPTDK 171
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor
1-alpha protein.
Length = 119
Score = 72.9 bits (171), Expect = 3e-15
Identities = 32/37 (86%), Positives = 35/37 (94%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KN+SVKELRRGYVAGDS+N PP+GAADFTAQVI LNH
Sbjct: 24 KNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNH 60
Score = 51.2 bits (117), Expect = 1e-08
Identities = 22/24 (91%), Positives = 23/24 (95%)
Frame = +1
Query: 529 KSVEMHHEALQEAVPGDNVGFNVK 600
KSVEMHHEAL EA+PGDNVGFNVK
Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVK 24
Score = 47.6 bits (108), Expect = 1e-07
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGC 773
PGQISNGY PVLDCHT HI C
Sbjct: 61 PGQISNGYTPVLDCHTAHIAC 81
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 241,609
Number of Sequences: 438
Number of extensions: 5303
Number of successful extensions: 21
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 25367793
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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