Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0870
(610 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 286 9e-78
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 285 1e-77
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 285 1e-77
At1g29760.1 68414.m03639 expressed protein 31 0.60
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 30 1.4
At2g38570.1 68415.m04738 expressed protein ; expression supporte... 30 1.4
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.4
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 4.2
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 4.2
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 4.2
At4g38440.1 68417.m05432 expressed protein 28 5.6
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 5.6
At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein... 27 9.7
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 286 bits (701), Expect = 9e-78
Identities = 130/185 (70%), Positives = 154/185 (83%)
Frame = +2
Query: 2 LNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVAT 361
VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQF 131
Query: 362 GPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGR 541
G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GR
Sbjct: 132 GQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGR 191
Query: 542 VGTIR 556
VG I+
Sbjct: 192 VGVIK 196
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 285 bits (700), Expect = 1e-77
Identities = 130/185 (70%), Positives = 154/185 (83%)
Frame = +2
Query: 2 LNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVAT 361
VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQF 131
Query: 362 GPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGR 541
G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GR
Sbjct: 132 GQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGR 191
Query: 542 VGTIR 556
VG I+
Sbjct: 192 VGVIK 196
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 285 bits (700), Expect = 1e-77
Identities = 130/185 (70%), Positives = 154/185 (83%)
Frame = +2
Query: 2 LNAPKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVAT 361
VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ +
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQF 131
Query: 362 GPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGR 541
G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GR
Sbjct: 132 GQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGR 191
Query: 542 VGTIR 556
VG I+
Sbjct: 192 VGVIK 196
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.60
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 204 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 112
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 29.9 bits (64), Expect = 1.4
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 93 KHSRNLWGPVDGLGAYTPPS 34
KHS +LWG D L TPP+
Sbjct: 44 KHSADLWGSADALAIATPPA 63
>At2g38570.1 68415.m04738 expressed protein ; expression supported
by MPSS
Length = 302
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +2
Query: 467 TKIMDFIKFESGNLCMITGGRNLGRVGTIRVPRE 568
T I+ +IK G + GGRN+G+V T R+ E
Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVSTYRLDVE 229
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.4
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 183 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 67
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 4.2
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 27.9 bits (59), Expect = 5.6
Identities = 24/99 (24%), Positives = 42/99 (42%), Gaps = 4/99 (4%)
Frame = +2
Query: 11 PKHGMLDKLGGVYAPRPSTGPHKLRECLPL----VIFLRNRLKYALTGNEVLKIVKQRLI 178
P H L + V++ + STGP + E L + V FL + G+ +V L+
Sbjct: 1145 PPHWFLSAISAVHSGKTSTGPPESTELLEVAKAGVFFLAGLESSSGFGSLPSPVVSVPLV 1204
Query: 179 KVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRF 295
K T MD++ + T L+ + ++ G+F
Sbjct: 1205 ---WKFHALSTVLLVGMDIIEDKNTRNLYNYLQELYGQF 1240
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.9 bits (59), Expect = 5.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 392 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 523
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
>At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein
contains Pfam profile: PF01068 ATP dependent DNA ligase
domain
Length = 1417
Score = 27.1 bits (57), Expect = 9.7
Identities = 33/138 (23%), Positives = 59/138 (42%), Gaps = 6/138 (4%)
Frame = +2
Query: 110 LRNRLKYALTGNEVLKIVKQRLIKVDGK-VRTDPTYPAGFMDVVSIEKTNEL--FRLIYD 280
LR +K+ L V+ V + K D K V + +G +D ++ +K L +R Y
Sbjct: 361 LREFIKF-LKPKRVIPTVGVDIEKFDCKEVNKMQKHFSGLVDEMANKKDFLLGFYRQSYQ 419
Query: 281 VKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYL---VTHDGRTIRYPDPLIKVNDSIQ 451
+ + ++ Y+ + G +NVP + HD LIK+ DS+
Sbjct: 420 KNEKSDVDVVSHSAEVYEEEEKNACEDGGENVPSSRGPILHDTTPSSDSRLLIKLRDSLP 479
Query: 452 LDIATTKIMDFIKFESGN 505
+ +++D IK +GN
Sbjct: 480 AWVTEEQMLDLIKKHAGN 497
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,257,752
Number of Sequences: 28952
Number of extensions: 305386
Number of successful extensions: 878
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 848
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -