Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1466
(734 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21810.1 68417.m03155 Der1-like family protein / degradation ... 52 4e-07
At4g04860.1 68417.m00708 Der1-like family protein / degradation ... 50 2e-06
At4g29330.1 68417.m04191 Der1-like family protein / degradation ... 44 8e-05
At5g22940.1 68418.m02682 exostosin family protein contains Pfam ... 33 0.26
At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOA... 30 1.4
At4g27020.1 68417.m03886 expressed protein gene F20P5.12 of BAC ... 30 1.8
At4g23160.1 68417.m03342 protein kinase family protein contains ... 29 4.2
At5g54870.1 68418.m06835 expressed protein strong similarity to ... 28 5.6
At5g42620.1 68418.m05188 expressed protein 28 7.4
At2g13650.2 68415.m01505 GDP-mannose transporter (GONST1) identi... 28 7.4
At2g13650.1 68415.m01504 GDP-mannose transporter (GONST1) identi... 28 7.4
At1g73590.1 68414.m08519 auxin efflux carrier protein, putative ... 28 7.4
At1g61670.1 68414.m06956 expressed protein similar to membrane p... 28 7.4
At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 27 9.8
At3g58460.1 68416.m06516 rhomboid family protein / ubiquitin-ass... 27 9.8
At1g28170.1 68414.m03458 sulfotransferase family protein similar... 27 9.8
>At4g21810.1 68417.m03155 Der1-like family protein / degradation in
the ER-like family protein contains Pfam profile:
PF04511 Der1-like family
Length = 244
Score = 52.0 bits (119), Expect = 4e-07
Identities = 30/108 (27%), Positives = 49/108 (45%)
Frame = +2
Query: 248 IPNWYNGVPFFTRYWMTFTIVLSLFGKFGLVSPYYFILDFYYFFNQFQIWRPLTALFYYP 427
+ WY +P TR ++T +V ++ ++SPY L+ Q+Q WR +T Y+
Sbjct: 5 VEEWYKQMPIITRSYLTAAVVTTVGCSLEIISPYNLYLNPTLVVKQYQFWRLVTNFLYF- 63
Query: 428 INPGTGFHFLINCYFLYNYSQRLETGMLQGSQLTISTCFCLIGCAVLS 571
FL + +FL Y + LE +G + T L G VL+
Sbjct: 64 --RKMDLDFLFHMFFLARYCKLLEENSFRG-KTTDFLYMLLFGATVLT 108
Score = 45.2 bits (102), Expect = 5e-05
Identities = 29/81 (35%), Positives = 41/81 (50%), Gaps = 11/81 (13%)
Frame = +1
Query: 514 GKPADYFYMLLFNWVCC---VIIGLLV--------KLPVLMDPMVLSVLYVWCQLNKDVI 660
GK D+ YMLLF V+IG ++ K+ L + + ++YVW + N +
Sbjct: 91 GKTTDFLYMLLFGATVLTGIVLIGGMIPYLSVSFSKIIFLSNSLTFMMVYVWSKQNPYIH 150
Query: 661 VSFWFGTRFKAMYLPWVLLAF 723
+SF F A YLPWVLL F
Sbjct: 151 MSFLGLFTFTAAYLPWVLLGF 171
>At4g04860.1 68417.m00708 Der1-like family protein / degradation in
the ER-like family protein contains Pfam profile:
PF04511 Der1-like family
Length = 244
Score = 50.0 bits (114), Expect = 2e-06
Identities = 27/108 (25%), Positives = 48/108 (44%)
Frame = +2
Query: 248 IPNWYNGVPFFTRYWMTFTIVLSLFGKFGLVSPYYFILDFYYFFNQFQIWRPLTALFYYP 427
+ WY +P TR ++T ++ ++ ++SPY L+ Q+Q WR +T Y+
Sbjct: 5 VEEWYKQMPIITRSYLTAAVITTVGCSLDIISPYNLYLNPTLVVKQYQYWRLVTNFLYF- 63
Query: 428 INPGTGFHFLINCYFLYNYSQRLETGMLQGSQLTISTCFCLIGCAVLS 571
F+ + +FL Y + LE +G L G +VL+
Sbjct: 64 --RKMDLDFMFHMFFLARYCKLLEENSFRGKTADF-LYMLLFGASVLT 108
Score = 46.4 bits (105), Expect = 2e-05
Identities = 30/81 (37%), Positives = 42/81 (51%), Gaps = 11/81 (13%)
Frame = +1
Query: 514 GKPADYFYMLLFN---WVCCVIIGLLV--------KLPVLMDPMVLSVLYVWCQLNKDVI 660
GK AD+ YMLLF V+IG ++ K+ L + + ++YVW + N +
Sbjct: 91 GKTADFLYMLLFGASVLTGIVLIGGMIPYLSASFAKIIFLSNSLTFMMVYVWSKQNPYIH 150
Query: 661 VSFWFGTRFKAMYLPWVLLAF 723
+SF F A YLPWVLL F
Sbjct: 151 MSFLGLFTFTAAYLPWVLLGF 171
>At4g29330.1 68417.m04191 Der1-like family protein / degradation in
the ER-like family protein contains Pfam profile:
PF04511 Der1-like family
Length = 266
Score = 44.4 bits (100), Expect = 8e-05
Identities = 26/83 (31%), Positives = 40/83 (48%)
Frame = +2
Query: 257 WYNGVPFFTRYWMTFTIVLSLFGKFGLVSPYYFILDFYYFFNQFQIWRPLTALFYYPINP 436
+YN +P T+ + T ++ + GLV+P + L QFQIWR +T LF+
Sbjct: 7 FYNSLPPITKAYGTLCFFTTVATQLGLVAPVHIALIPELVLKQFQIWRLITNLFFL---G 63
Query: 437 GTGFHFLINCYFLYNYSQRLETG 505
G +F I + Y +LE G
Sbjct: 64 GFSINFGIRLLMIARYGVQLEKG 86
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 2/70 (2%)
Frame = +1
Query: 517 KPADYFYMLLFNWVCCVIIGLLVKL--PVLMDPMVLSVLYVWCQLNKDVIVSFWFGTRFK 690
+ AD+ +M++F +++ ++ P L +V +LY+W + + +S + K
Sbjct: 91 RTADFLWMMIFGSFTLLVLSVIPFFWTPFLGVSLVFMLLYLWSREFPNANISLYGLVTLK 150
Query: 691 AMYLPWVLLA 720
A YLPW +LA
Sbjct: 151 AFYLPWAMLA 160
>At5g22940.1 68418.m02682 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 469
Score = 32.7 bits (71), Expect = 0.26
Identities = 18/58 (31%), Positives = 30/58 (51%)
Frame = +1
Query: 298 FYYCIEFVW*IWACEPLLFYTGFLLFLQSISDMASINSFVLLSNKSRHRISFPDKLLL 471
F++ + W +W C L F+T + S+ D +S +S LLSN + S P + L+
Sbjct: 41 FFFRNYYTWILWFCLSLYFFTSYF----SVEDQSSPSSIRLLSN-HKTSSSLPSRALI 93
>At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOAT)
family protein low similarity to porcupine from [Xenopus
laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516;
contains Pfam profile PF03062: MBOAT family
Length = 465
Score = 30.3 bits (65), Expect = 1.4
Identities = 17/79 (21%), Positives = 34/79 (43%)
Frame = +2
Query: 329 FGLVSPYYFILDFYYFFNQFQIWRPLTALFYYPINPGTGFHFLINCYFLYNYSQRLETGM 508
FG S +F++ + I+RPL+ + + GF +LI C+ Y + G
Sbjct: 61 FGFSSNLHFLVPMTIGYASMAIYRPLSGFITFFL----GFAYLIGCHVFYMSGDAWKEGG 116
Query: 509 LQGSQLTISTCFCLIGCAV 565
+ + + +I C++
Sbjct: 117 IDSTGALMVLTLKVISCSI 135
>At4g27020.1 68417.m03886 expressed protein gene F20P5.12 of BAC
F20P5 from Arabidopsis thalianachromosome 1,
PID:g2194125
Length = 523
Score = 29.9 bits (64), Expect = 1.8
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = +1
Query: 502 RHVAGKPADYFYMLLFNWVCCVIIGLLVKLPVLMDPMVLSVLYVWCQLNKDVIVSFW 672
R + GKP Y + L+F+W+ I G P L +V SV+ VW ++ D + W
Sbjct: 322 RSMDGKPYGY-HNLIFSWID-TISGNYP--PPLDAQLVASVMTVWSKIQPDYAANMW 374
>At4g23160.1 68417.m03342 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 1262
Score = 28.7 bits (61), Expect = 4.2
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = +3
Query: 108 HNDHTIFMXXXXXXXXXXXTDIYDIVLCNNKSVCANEIIRKSWKMSEFRI 257
H+DHT F+ + DI++C+N +E+ KS S F++
Sbjct: 263 HSDHTYFLKITATLFLCVLVYVDDIIICSNNDAAVDEL--KSQLKSCFKL 310
>At5g54870.1 68418.m06835 expressed protein strong similarity to
unknown protein (pir||T04825)
Length = 531
Score = 28.3 bits (60), Expect = 5.6
Identities = 17/57 (29%), Positives = 27/57 (47%)
Frame = +1
Query: 502 RHVAGKPADYFYMLLFNWVCCVIIGLLVKLPVLMDPMVLSVLYVWCQLNKDVIVSFW 672
R + GKP Y + L+F+W+ V P L +V S + VW Q+ + + W
Sbjct: 330 RSMEGKPYGY-HNLIFSWIDTVSENYP---PPLDAHLVASFMTVWSQMQPEYAANMW 382
>At5g42620.1 68418.m05188 expressed protein
Length = 841
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +2
Query: 476 YNYSQRLETGMLQGSQLTISTCFCLIGCAVLSLDYLSNCQY 598
Y+ + RL+ G QG+Q S C G + LS C Y
Sbjct: 412 YSMADRLDWGRNQGTQFVTSPCNMWKGAYHCNTTQLSGCTY 452
>At2g13650.2 68415.m01505 GDP-mannose transporter (GONST1) identical
to GDP-mannose transporter SP:Q941R4 from [Arabidopsis
thaliana]
Length = 284
Score = 27.9 bits (59), Expect = 7.4
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +1
Query: 523 ADYFYMLLFNWVCCVII-GLLVKLPVLMDPMVLSVLYVWCQLNKDVIVSFWFGTRFKAMY 699
A F ML N+V +I+ GL + + +P+ L ++ VW +N + V + F Y
Sbjct: 62 AGIFLMLYQNFVSVIIVVGLSLMGLITTEPLTLRLMKVWFPVNV-IFVGMLITSMFSLKY 120
Query: 700 L 702
+
Sbjct: 121 I 121
>At2g13650.1 68415.m01504 GDP-mannose transporter (GONST1) identical
to GDP-mannose transporter SP:Q941R4 from [Arabidopsis
thaliana]
Length = 333
Score = 27.9 bits (59), Expect = 7.4
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +1
Query: 523 ADYFYMLLFNWVCCVII-GLLVKLPVLMDPMVLSVLYVWCQLNKDVIVSFWFGTRFKAMY 699
A F ML N+V +I+ GL + + +P+ L ++ VW +N + V + F Y
Sbjct: 62 AGIFLMLYQNFVSVIIVVGLSLMGLITTEPLTLRLMKVWFPVNV-IFVGMLITSMFSLKY 120
Query: 700 L 702
+
Sbjct: 121 I 121
>At1g73590.1 68414.m08519 auxin efflux carrier protein, putative
(PIN1) identical to putative auxin efflux carrier
protein; AtPIN1 [Arabidopsis thaliana] GI:4151319;
contains Pfam profile PF03547: Auxin Efflux Carrier
Length = 622
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +1
Query: 598 LMDPMVLSVLYVWCQLNKDVIVSFWFGTRFKAMYLPWVLL 717
L +VLS+L++WC+L+++ + W T F LP L+
Sbjct: 77 LQKVIVLSLLFLWCKLSRNGSLD-WTITLFSLSTLPNTLV 115
>At1g61670.1 68414.m06956 expressed protein similar to membrane
protein PTM1 precursor isolog GI:1931644 from
[Arabidopsis thaliana]
Length = 513
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +2
Query: 308 VLSLFGKFGLVSPYYFILDFYYFFNQFQIWRPLTALFYY 424
V L FG +S Y +L +F Q W+ + L Y+
Sbjct: 216 VAPLMNVFGFMSLAYILLGLVWFVRFVQFWKDIIQLHYH 254
>At3g61670.1 68416.m06911 expressed protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 790
Score = 27.5 bits (58), Expect = 9.8
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = +2
Query: 425 PINPGTGFHFLINCYFLYNYSQRLETGMLQGSQLTISTCFCLIGCAVLS 571
P+ G F NC+ L ++ E G + ++ C CLI +V++
Sbjct: 415 PLAGGAPFITCQNCFELLQLPKKPEAGTKKQQKVRCGACSCLIDLSVVN 463
>At3g58460.1 68416.m06516 rhomboid family protein /
ubiquitin-associated (UBA)/TS-N domain-containing
protein contains Pfam profiles PF01694: Rhomboid family,
PF00627: UBA/TS-N domain
Length = 403
Score = 27.5 bits (58), Expect = 9.8
Identities = 20/85 (23%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
Frame = +2
Query: 248 IPNWYNGVPFFTRYWMTFTIVLSLFGKFGLVSPYYFILDF-YYFFNQFQIWRPLTALFYY 424
+ W+N +PF T + V+ L +Y + ++FQ++R TA+ ++
Sbjct: 15 VGQWWNAIPFLTSSVVVVCGVIYLICLLTGYDTFYEVCFLPSAIISRFQVYRFYTAIIFH 74
Query: 425 PINPGTGFHFLINCYFLYNYSQRLE 499
G+ H L N L LE
Sbjct: 75 ----GSLLHVLFNMMALVPMGSELE 95
>At1g28170.1 68414.m03458 sulfotransferase family protein similar to
steroid sulfotransferase 3 GI:3420008 from [Brassica
napus]; contains Pfam profile PF00685: Sulfotransferase
domain
Length = 326
Score = 27.5 bits (58), Expect = 9.8
Identities = 10/18 (55%), Positives = 15/18 (83%)
Frame = +1
Query: 622 VLYVWCQLNKDVIVSFWF 675
++YVW + KDV+VSFW+
Sbjct: 155 IVYVWRNV-KDVLVSFWY 171
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,825,811
Number of Sequences: 28952
Number of extensions: 337779
Number of successful extensions: 811
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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