Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--1461
(760 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 78 7e-15
At5g02880.1 68418.m00231 HECT-domain-containing protein / ubiqui... 75 5e-14
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui... 73 3e-13
At3g17205.1 68416.m02196 HECT-domain-containing protein / ubiqui... 45 6e-05
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 42 3e-04
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 42 4e-04
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 39 0.004
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 33 0.27
At5g57500.1 68418.m07185 expressed protein 30 1.5
At5g18880.1 68418.m02243 expressed protein ; expression supporte... 29 4.4
At3g59770.1 68416.m06670 sacI homology domain-containing protein... 28 7.7
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 77.8 bits (183), Expect = 7e-15
Identities = 37/73 (50%), Positives = 49/73 (67%)
Frame = +2
Query: 23 GRDQRRDPRMLAE*MRPDHGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
GR + + LAE ++ DHGYNA+S I L++I+ ++QR F QFVTG+PRLP GG
Sbjct: 1755 GRRELWEVETLAEHIKFDHGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGG 1814
Query: 203 FKALNPPLTVVRK 241
LNP LT+VRK
Sbjct: 1815 LAVLNPKLTIVRK 1827
Score = 63.3 bits (147), Expect = 2e-10
Identities = 33/59 (55%), Positives = 39/59 (66%)
Frame = +1
Query: 217 STTNCGA*IAGVLLDPDEYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
ST++ A AG D+ LPSVMTC NYLKLP YS+ E+M KL A +EGQ SF LS
Sbjct: 1830 STSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1888
>At5g02880.1 68418.m00231 HECT-domain-containing protein /
ubiquitin-transferase family protein /
armadillo/beta-catenin-like repeat-containing protein
similar to SP|Q14669 Thyroid receptor interacting protein
12 (TRIP12) {Homo sapiens}; contains Pfam profiles
PF00632: HECT-domain (ubiquitin-transferase), PF00514:
Armadillo/beta-catenin-like repeat
Length = 1502
Score = 74.9 bits (176), Expect = 5e-14
Identities = 33/56 (58%), Positives = 42/56 (75%)
Frame = +2
Query: 74 DHGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTVVRK 241
DHGY + S + L+ IL ++RE+QR FLQFVTGSPRLP GG +L+P LT+VRK
Sbjct: 1398 DHGYTSSSPPVEYLLQILHEFDREQQRAFLQFVTGSPRLPHGGLASLSPKLTIVRK 1453
Score = 62.1 bits (144), Expect = 4e-10
Identities = 30/43 (69%), Positives = 32/43 (74%)
Frame = +1
Query: 265 DEYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
D LPSVMTC NYLKLP YSS E M+ KL A +EGQ SFHLS
Sbjct: 1460 DTDLPSVMTCANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502
>At4g38600.2 68417.m05463 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
{Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1794
Score = 72.5 bits (170), Expect = 3e-13
Identities = 37/73 (50%), Positives = 48/73 (65%)
Frame = +2
Query: 23 GRDQRRDPRMLAE*MRPDHGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGG 202
GR + + LAE ++ DHGYNA+S I I+I+ ++QR F QFVTG+PRLP GG
Sbjct: 1664 GRRELWEVETLAEHIKFDHGYNAKSPAI---INIMGELTADQQRAFCQFVTGAPRLPPGG 1720
Query: 203 FKALNPPLTVVRK 241
LNP LT+VRK
Sbjct: 1721 LAVLNPKLTIVRK 1733
Score = 63.3 bits (147), Expect = 2e-10
Identities = 33/59 (55%), Positives = 39/59 (66%)
Frame = +1
Query: 217 STTNCGA*IAGVLLDPDEYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
ST++ A AG D+ LPSVMTC NYLKLP YS+ E+M KL A +EGQ SF LS
Sbjct: 1736 STSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 1794
>At3g17205.1 68416.m02196 HECT-domain-containing protein /
ubiquitin-transferase family protein weak similarity to
ubiquitin-protein ligase 2 [Arabidopsis thaliana]
GI:7108523; contains Pfam profile PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 44.8 bits (101), Expect = 6e-05
Identities = 22/53 (41%), Positives = 31/53 (58%)
Frame = +2
Query: 80 GYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTVVR 238
GY+A I M +++ S++ E Q+ FL+FVTG R P GFK L P + R
Sbjct: 770 GYHAGHYVIDMFWEVMKSFSTENQKKFLKFVTGCSRGPLLGFKYLEPAFCIQR 822
Score = 37.1 bits (82), Expect = 0.013
Identities = 19/40 (47%), Positives = 24/40 (60%)
Frame = +1
Query: 274 LPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
LP+ TC+N LKLP Y S E++ KL A S + F LS
Sbjct: 835 LPTSATCMNLLKLPPYQSKELLETKLMYAIS-AEAGFDLS 873
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 42.3 bits (95), Expect = 3e-04
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +2
Query: 83 YNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
Y S IR +++ ++++E+ FLQFVTG+ ++P GFKAL
Sbjct: 3555 YTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3598
Score = 38.3 bits (85), Expect = 0.005
Identities = 18/39 (46%), Positives = 22/39 (56%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSF 384
E LPS TC N L LP+Y S E ++ +L LA E F
Sbjct: 3617 ERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGF 3655
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 41.9 bits (94), Expect = 4e-04
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +2
Query: 83 YNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKAL 214
Y A S I +++ ++++E+ FLQFVTG+ ++P GFKAL
Sbjct: 3788 YTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3831
Score = 38.7 bits (86), Expect = 0.004
Identities = 18/39 (46%), Positives = 22/39 (56%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSF 384
E LPS TC N L LP+Y S E ++ +L LA E F
Sbjct: 3850 ERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGF 3888
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains Pfam
profiles PF00632: HECT-domain (ubiquitin-transferase),
PF00612: IQ calmodulin-binding motif
Length = 1142
Score = 38.7 bits (86), Expect = 0.004
Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = +2
Query: 80 GYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTVVRKSLE-SY 256
GY+ SR I++ +++ + E+ L+FVT R P GFK L P + + S + S
Sbjct: 1033 GYSDSSRTIKIFWEVMKGFEPSERCLLLKFVTSCSRAPLLGFKYLQPTFIIHKVSCDTSL 1092
Query: 257 W 259
W
Sbjct: 1093 W 1093
Score = 38.3 bits (85), Expect = 0.005
Identities = 22/42 (52%), Positives = 22/42 (52%)
Frame = +1
Query: 268 EYLPSVMTCVNYLKLPDYSSAEVMRAKLRLAASEGQHSFHLS 393
E LPS TC N LKLP Y A MR KL L A F LS
Sbjct: 1102 ERLPSASTCYNTLKLPTYKRASTMREKL-LYAITSNAGFELS 1142
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 32.7 bits (71), Expect = 0.27
Identities = 18/54 (33%), Positives = 25/54 (46%)
Frame = +2
Query: 77 HGYNAESRGIRMLIDILASYNREEQRHFLQFVTGSPRLPTGGFKALNPPLTVVR 238
+G+ R I IL EEQR L F T + +P GF+ L+ L + R
Sbjct: 772 NGFKETDRQIDWFWKILKKMTEEEQRSILFFWTSNKFVPVEGFRGLSSKLYIYR 825
>At5g57500.1 68418.m07185 expressed protein
Length = 318
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +2
Query: 104 IRMLIDILASYNREEQRHFLQFVTGSPRLPTG 199
IR+LI IL ++ +RHFL+ + G+ +P G
Sbjct: 69 IRILIGILTLPDQYSRRHFLRMIYGTQNVPDG 100
>At5g18880.1 68418.m02243 expressed protein ; expression supported
by MPSS
Length = 295
Score = 28.7 bits (61), Expect = 4.4
Identities = 12/32 (37%), Positives = 15/32 (46%)
Frame = +1
Query: 31 PTTGSTYAGGMNASGSWIQCRIAGYTHAY*YF 126
PT GMN SW+ C THA+ +F
Sbjct: 153 PTRDRLRGWGMNIPSSWVLCSNGDETHAHLFF 184
>At3g59770.1 68416.m06670 sacI homology domain-containing protein / WW
domain-containing protein contains Pfam profiles PF00397:
WW domain, PF02383: SacI homology domain; identical to
cDNA SAC domain protein 9 (SAC9) GI:31415734
Length = 1630
Score = 27.9 bits (59), Expect = 7.7
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = -2
Query: 237 RTTVSGGFNALNPPVGSLGLPVTNCKKCRCSSRLYEAKISISMRI 103
R T +GG + + S+G CKKC CSS + EA I +R+
Sbjct: 1136 RDTANGGGSLADVSATSIGSDHYICKKC-CSSIVLEALIVDYVRV 1179
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,224,415
Number of Sequences: 28952
Number of extensions: 358559
Number of successful extensions: 749
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 719
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 748
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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