BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--1454 (756 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g20010.1 68415.m02339 expressed protein 31 1.1 At5g52180.1 68418.m06477 expressed protein 30 1.4 At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 famil... 28 5.8 >At2g20010.1 68415.m02339 expressed protein Length = 834 Score = 30.7 bits (66), Expect = 1.1 Identities = 14/31 (45%), Positives = 20/31 (64%) Frame = -3 Query: 751 RCLKMFSASVHDTTQNRVSQLLIRYRKDRFL 659 RCL++ S+SVHD + RV ++R D FL Sbjct: 677 RCLEIISSSVHDRVRTRVISDIMRASFDGFL 707 >At5g52180.1 68418.m06477 expressed protein Length = 458 Score = 30.3 bits (65), Expect = 1.4 Identities = 17/37 (45%), Positives = 24/37 (64%) Frame = +3 Query: 639 LNLLASYKNLSFLYLISSWLTLF*VVSWTEAENIFKH 749 L LL SY+NL+ L+S +LTL V+S+ + IF H Sbjct: 2 LELLGSYRNLTLQILLSLFLTL--VLSFVKISIIFLH 36 >At2g03820.1 68415.m00343 nonsense-mediated mRNA decay NMD3 family protein contains Pfam profile: PF04981 NMD3 family Length = 516 Score = 28.3 bits (60), Expect = 5.8 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = -1 Query: 90 QQHLSTSAGSNLFNSKYHYNIRGCPL 13 QQ +S S+L+N KY Y+++ CP+ Sbjct: 228 QQLVSHDVKSSLYNYKYTYSVKICPV 253 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,949,187 Number of Sequences: 28952 Number of extensions: 246627 Number of successful extensions: 412 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 403 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 412 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1682736544 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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